scientific ltq orbitrap mass spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher scientific ltq orbitrap mass spectrometer
    Scientific Ltq Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scientific ltq orbitrap mass spectrometer/product/Thermo Fisher
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    scientific ltq orbitrap mass spectrometer - by Bioz Stars, 2020-05
    90/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Selenium species-dependent toxicity, bioavailability and metabolic transformations in Caenorhabditis elegans
    Article Snippet: .. For identification of selenium metabolites the HPLC system was coupled to a Q-Exactive Orbitrap Mass Spectrometer (Thermo Scientific, Waltham, USA) equipped with a heated electrospray ionization (HESI2) source from the same manufacturer. .. MS was performed in the positive mode and source settings were: gas temp 400°C, flow rates 47 (sheath) and 11 (aux) instrument units, spray voltage 3500 V and capillary temp 250°C.

    Liquid Chromatography:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Mass Spectrometry:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Article Title: Serial-omics of P53−/−, Brca1−/− Mouse Breast Tumor and Normal Mammary Gland
    Article Snippet: .. Lipids were analyzed using a high resolution hybrid QExactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific) in DDA mode (Top 8) using positive/negative ion polarity switching. ..

    Article Title: Selenium species-dependent toxicity, bioavailability and metabolic transformations in Caenorhabditis elegans
    Article Snippet: .. For identification of selenium metabolites the HPLC system was coupled to a Q-Exactive Orbitrap Mass Spectrometer (Thermo Scientific, Waltham, USA) equipped with a heated electrospray ionization (HESI2) source from the same manufacturer. .. MS was performed in the positive mode and source settings were: gas temp 400°C, flow rates 47 (sheath) and 11 (aux) instrument units, spray voltage 3500 V and capillary temp 250°C.

    Article Title: Quantitative Paper Spray Mass Spectrometry Analysis of Drugs of Abuse
    Article Snippet: .. The MS spectra were recorded using an Exactive Orbitrap mass spectrometer (Exactive, Thermo Scientific, CA). .. Quantitation of drugs in blood was carried out by tandem mass spectrometry (MS/MS) using a TSQ Quantum Access Max mass spectrometer (Thermo Scientific, CA) in the multiple reaction monitoring (MRM) mode with an isolation widow width of m/z 0.4.

    Article Title: Purification and Characterization of Latent Polyphenol Oxidase from Apricot (Prunus armeniaca L.)
    Article Snippet: .. Tryptic digestions of Pa PPO were analyzed by nanoUHPLC–ESI–MS/MS using a high-resolution Orbitrap mass spectrometer (Dionex Ultimate 3000 RSLCnano, Q Exactive Orbitrap, Thermo Scientific). .. The data analysis was performed with Proteome Discoverer 1.4 by searching against the P. armeniaca entries from the UniProt database (containing all listed proteins from P. mume , P. persica , and P. armeniaca ).

    Article Title: The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes
    Article Snippet: .. In 2012, Heck et al . first demonstrated the use of an Orbitrap mass spectrometer (Exactive Plus™, Thermo Fisher Scientific) for the analysis of intact protein complexes ranging from 150 kDa to 800 kDa. .. – They subsequently extended this range to include virus-like nanoparticles with a mass up to 4.5 MDa ( ).

    Article Title: Application of high-resolution mass spectrometry to measure low abundance isotope enrichment in individual muscle proteins
    Article Snippet: .. Analysis of [ring-13 C6 ]-phenylalanine enrichment was performed with a high-resolution orbitrap mass spectrometer (Thermo Q Exactive Plus) using a 14 min targeted MS/MS method. .. The orbitrap MS was operated at 70,000 resolution, with an AGC target of 5e6 and maximum injection time of 100 ms. An isolation width of 1.0 m/z was applied for isolation of the m+3 and m+6 precursor species at m/z 225.15 and 228.17, respectively, and MS/MS was performed in the HCD cell at 40 V. All resulting fragments within the m/z 50-250 window were detected in the orbitrap.

    Article Title: Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery
    Article Snippet: .. These fractions were desalted using C18 OMIX® tips (Agilent) and analysed on a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) coupled to an EASY-nLC1000 (Thermo Scientific). ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: AZA-MS: a novel multiparameter mass spectrometry method to determine the intracellular dynamics of azacitidine therapy in vivo
    Article Snippet: .. Mass spectrometry and data analysis LC–MS analysis was performed utilising an ultra-high-performance liquid chromatography system (Dionex u3000 system, Thermo Fisher Scientific) interfaced to an Orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific) using a heated electrospray interface operated in the positive ion mode. .. The chromatographic separation was performed on a 100 mm × 2.1 mm i.d., 3 μM , C30 column (Acclaim, Thermo Fisher Scientific) kept at 40 ºC.

    Tandem Mass Spectroscopy:

    Article Title: Application of high-resolution mass spectrometry to measure low abundance isotope enrichment in individual muscle proteins
    Article Snippet: .. Analysis of [ring-13 C6 ]-phenylalanine enrichment was performed with a high-resolution orbitrap mass spectrometer (Thermo Q Exactive Plus) using a 14 min targeted MS/MS method. .. The orbitrap MS was operated at 70,000 resolution, with an AGC target of 5e6 and maximum injection time of 100 ms. An isolation width of 1.0 m/z was applied for isolation of the m+3 and m+6 precursor species at m/z 225.15 and 228.17, respectively, and MS/MS was performed in the HCD cell at 40 V. All resulting fragments within the m/z 50-250 window were detected in the orbitrap.

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    Thermo Fisher ltq orbitrap velos mass spectrometer
    A schematic workflow illustrating the steps involved in the differential analysis of RA and OA synovial fluid proteome. Proteins from RA and OA synovial fluid were extracted and depleted to remove the 14 most abundant proteins using multiple affinity removal system, Human-14. The depleted protein from RA and OA were then digested with trypsin and labeled with iTRAQ reagents, 117 and 116 respectively. The labeled samples were pooled and subjected to fractionation using strong cation exchange chromatography. The fractions were then analyzed on a <t>LTQ-Orbitrap</t> <t>Velos</t> mass spectrometer. The MS/MS data obtained was searched against Human RefSeq 50 database using Sequest and Mascot search algorithms. Validation of the iTRAQ quantitation data was carried out using multiple reaction monitoring and Western blot.
    Ltq Orbitrap Velos Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap velos mass spectrometer/product/Thermo Fisher
    Average 93 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap velos mass spectrometer - by Bioz Stars, 2020-05
    93/100 stars
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    93
    Thermo Fisher ltq orbitrap xl mass spectrometer
    (A) Scheme of the methods used to analyze BMSCs Proteins identification was done on an <t>orbitrap</t> <t>LTQ</t> XL with a protein threshold of 99% (FDR 0.1%) and peptide threshold of 97% (FDR 0.1%) and contained at least 2 identified peptides as parameters ( Supplementary data 1 2 ). (B) Label free quantification of immune modulators, neurotrophic and growth factors and apoptotic molecules identified in conditioned media obtained from BMSCs, spinal cord lesion (SCI) or control (non injured) using Scaffold_4.0.6.1. Label-free quantification was done on top3 of the total ion current (TIC). (BMP 1: Bone morphogenic protein 1, Cdh13: T-Cadherin, CLEC11a: C-type lectin domain family 11 member A CTGF: connective tissue growth factor, Epha 3: Ephrin A isoform 3, Epdr1: Ependymin related protein 1, FAM3C: family with sequence similarity 3, member C Gal 1: galectin 1; GAP-43: Growth associated protein 43, Ltbp: Latent TGF-beta binding protein, IgFbp 7: Insulin growth factor binding protein 7, MIF: macrophage inhibiting factor, PDGF: Platelet derived growth factor, PGF: placenta growth factor, spondin 2, SPARC, TIMP-1: Tissue inhibitor metalloproteinase 1 2).
    Ltq Orbitrap Xl Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap xl mass spectrometer/product/Thermo Fisher
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap xl mass spectrometer - by Bioz Stars, 2020-05
    93/100 stars
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    85
    Thermo Fisher high mass accuracy high resolution mass spectrometer
    Validation of very low abundance cardiolipin isomeric species by product ion analysis using an Orbitrap-LTQ <t>mass</t> <t>spectrometer.</t> Product ion mass spectra of representative myocardial cardiolipin species were acquired using a <t>high</t> mass <t>accuracy/resolution</t>
    High Mass Accuracy High Resolution Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high mass accuracy high resolution mass spectrometer/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high mass accuracy high resolution mass spectrometer - by Bioz Stars, 2020-05
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    88
    Thermo Fisher ltq orbitrap classic mass spectrometer
    SILAC workflow. A combined strategy involving RNAi against Cdc7, SDS-PAGE protein fractionation, LC–MS/MS data acquisition with a <t>LTQ-Orbitrap</t> (Thermo Fisher Scientific), and downstream bioinformatics for identification, quantification, and functional annotation was applied in this study.
    Ltq Orbitrap Classic Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq orbitrap classic mass spectrometer/product/Thermo Fisher
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ltq orbitrap classic mass spectrometer - by Bioz Stars, 2020-05
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    Image Search Results


    A schematic workflow illustrating the steps involved in the differential analysis of RA and OA synovial fluid proteome. Proteins from RA and OA synovial fluid were extracted and depleted to remove the 14 most abundant proteins using multiple affinity removal system, Human-14. The depleted protein from RA and OA were then digested with trypsin and labeled with iTRAQ reagents, 117 and 116 respectively. The labeled samples were pooled and subjected to fractionation using strong cation exchange chromatography. The fractions were then analyzed on a LTQ-Orbitrap Velos mass spectrometer. The MS/MS data obtained was searched against Human RefSeq 50 database using Sequest and Mascot search algorithms. Validation of the iTRAQ quantitation data was carried out using multiple reaction monitoring and Western blot.

    Journal: Clinical proteomics

    Article Title: Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients

    doi: 10.1186/1559-0275-11-1

    Figure Lengend Snippet: A schematic workflow illustrating the steps involved in the differential analysis of RA and OA synovial fluid proteome. Proteins from RA and OA synovial fluid were extracted and depleted to remove the 14 most abundant proteins using multiple affinity removal system, Human-14. The depleted protein from RA and OA were then digested with trypsin and labeled with iTRAQ reagents, 117 and 116 respectively. The labeled samples were pooled and subjected to fractionation using strong cation exchange chromatography. The fractions were then analyzed on a LTQ-Orbitrap Velos mass spectrometer. The MS/MS data obtained was searched against Human RefSeq 50 database using Sequest and Mascot search algorithms. Validation of the iTRAQ quantitation data was carried out using multiple reaction monitoring and Western blot.

    Article Snippet: LC-MS/MS analysis Tandem mass spectrometric analysis of the iTRAQ labeled peptides were carried out using LTQ-Orbitrap Velos mass spectrometer (Thermo Scientific, Bremen, Germany) interfaced with Easy nanoLC II (previously Proxeon, Thermo Scientific, Bremen, Germany).

    Techniques: Labeling, Fractionation, Chromatography, Mass Spectrometry, Quantitation Assay, Western Blot

    (A) Scheme of the methods used to analyze BMSCs Proteins identification was done on an orbitrap LTQ XL with a protein threshold of 99% (FDR 0.1%) and peptide threshold of 97% (FDR 0.1%) and contained at least 2 identified peptides as parameters ( Supplementary data 1 2 ). (B) Label free quantification of immune modulators, neurotrophic and growth factors and apoptotic molecules identified in conditioned media obtained from BMSCs, spinal cord lesion (SCI) or control (non injured) using Scaffold_4.0.6.1. Label-free quantification was done on top3 of the total ion current (TIC). (BMP 1: Bone morphogenic protein 1, Cdh13: T-Cadherin, CLEC11a: C-type lectin domain family 11 member A CTGF: connective tissue growth factor, Epha 3: Ephrin A isoform 3, Epdr1: Ependymin related protein 1, FAM3C: family with sequence similarity 3, member C Gal 1: galectin 1; GAP-43: Growth associated protein 43, Ltbp: Latent TGF-beta binding protein, IgFbp 7: Insulin growth factor binding protein 7, MIF: macrophage inhibiting factor, PDGF: Platelet derived growth factor, PGF: placenta growth factor, spondin 2, SPARC, TIMP-1: Tissue inhibitor metalloproteinase 1 2).

    Journal: Scientific Reports

    Article Title: Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study

    doi: 10.1038/srep07514

    Figure Lengend Snippet: (A) Scheme of the methods used to analyze BMSCs Proteins identification was done on an orbitrap LTQ XL with a protein threshold of 99% (FDR 0.1%) and peptide threshold of 97% (FDR 0.1%) and contained at least 2 identified peptides as parameters ( Supplementary data 1 2 ). (B) Label free quantification of immune modulators, neurotrophic and growth factors and apoptotic molecules identified in conditioned media obtained from BMSCs, spinal cord lesion (SCI) or control (non injured) using Scaffold_4.0.6.1. Label-free quantification was done on top3 of the total ion current (TIC). (BMP 1: Bone morphogenic protein 1, Cdh13: T-Cadherin, CLEC11a: C-type lectin domain family 11 member A CTGF: connective tissue growth factor, Epha 3: Ephrin A isoform 3, Epdr1: Ependymin related protein 1, FAM3C: family with sequence similarity 3, member C Gal 1: galectin 1; GAP-43: Growth associated protein 43, Ltbp: Latent TGF-beta binding protein, IgFbp 7: Insulin growth factor binding protein 7, MIF: macrophage inhibiting factor, PDGF: Platelet derived growth factor, PGF: placenta growth factor, spondin 2, SPARC, TIMP-1: Tissue inhibitor metalloproteinase 1 2).

    Article Snippet: The chromatography system was coupled to a Thermo Scientific LTQ-Orbitrap XL mass spectrometer programmed to acquire in data-dependent mode.

    Techniques: Sequencing, Binding Assay, Derivative Assay

    Validation of very low abundance cardiolipin isomeric species by product ion analysis using an Orbitrap-LTQ mass spectrometer. Product ion mass spectra of representative myocardial cardiolipin species were acquired using a high mass accuracy/resolution

    Journal: Journal of Lipid Research

    Article Title: Dynamic simulation of cardiolipin remodeling: greasing the wheels for an interpretative approach to lipidomics [S]

    doi: 10.1194/jlr.M004796

    Figure Lengend Snippet: Validation of very low abundance cardiolipin isomeric species by product ion analysis using an Orbitrap-LTQ mass spectrometer. Product ion mass spectra of representative myocardial cardiolipin species were acquired using a high mass accuracy/resolution

    Article Snippet: Product-ion analyses of cardiolipin molecular species by using a high mass accuracy/high resolution mass spectrometer [i.e., Orbitrap-LTQ mass spectrometer (Thermo Scientific San Jose, CA)] were also preformed as previously described ( ) to verify the presence of very low abundance cardiolipin isomers.

    Techniques: Mass Spectrometry

    SILAC workflow. A combined strategy involving RNAi against Cdc7, SDS-PAGE protein fractionation, LC–MS/MS data acquisition with a LTQ-Orbitrap (Thermo Fisher Scientific), and downstream bioinformatics for identification, quantification, and functional annotation was applied in this study.

    Journal: Journal of proteome research

    Article Title: Quantitative Proteomics Reveals a “Poised Quiescence” Cellular State after Triggering the DNA Replication Origin Activation Checkpoint

    doi: 10.1021/pr100678k

    Figure Lengend Snippet: SILAC workflow. A combined strategy involving RNAi against Cdc7, SDS-PAGE protein fractionation, LC–MS/MS data acquisition with a LTQ-Orbitrap (Thermo Fisher Scientific), and downstream bioinformatics for identification, quantification, and functional annotation was applied in this study.

    Article Snippet: LC–MS/MS was performed with an LTQ-Orbitrap Classic mass spectrometer (Thermo Fisher Scientific, U.K.).

    Techniques: SDS Page, Fractionation, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Functional Assay