Journal: Bioengineering
Article Title: The Nitrogen Content in the Fruiting Body and Mycelium of Pleurotus Ostreatus and Its Utilization as a Medium Component in Thraustochytrid Fermentation
doi: 10.3390/bioengineering11030284
Figure Lengend Snippet: Effect of the substitution of complex nitrogen sources with a mycelium extract on the biomass formation and squalene accumulation in Schizochytrium sp. S31. The M50 medium is depicted in blue, the M50 medium with additional complex nitrogen source is depicted in red (M50 + N), the M50 medium without a complex nitrogen source is depicted in black (M50 − N), the mycelium extract with added glucose, NaCl and carbenicillin is depicted in white (E) and the M50 medium with the mycelium extract substituting the complex nitrogen source is depicted in grey (M50 − N + E). ( A ) Dry matter, ( B ) glucose concentration, ( C ) squalene content per gram of dry matter, ( D ) squalene yield per liter of cultivation volume and ( E ) pH over cultivation time. Data presented are the means of triplicates ± standard deviations. Significant differences ( p < 0.05) were denoted by lowercase letters depicted in (one-way ANOVA; Tukey post hoc test).
Article Snippet: Schizochytrium sp. S31 (American Type culture collection (ATCC) 20888) was preserved in 25% ( v / v ) glycerol with a preculture medium (PM, 10 g d -glucose, 1 g yeast extract, 1 g peptone ex casein, 12.5 g NaCl, 0.5 g KCl, 2.5 g MgSO 4 · 7 H 2 O, 1 g monosodium glutamate, 6 mg ammonium iron (III) citrate, 51.5 mg CaCl 2 , 30.4 mg K 2 HPO 4 , 2.86 mg H 3 BO 3 , 1.81 mg MnCl 2 · 4 H 2 O, 0.222 mg ZnSO 4 · 7 H 2 O, 0.39 mg Na 2 MoO 4 · 2 H 2 O, 0.079 mg CuSO 4 · 2 H 2 O, 0.0477 mg CoSO 4 · 7 H 2 O or 0.0494 mg Co(NO 3 ) 2 · 6 H 2 O, 0.1 mg carbenicillin, 595.8 mg HEPES, 0.5 µg biotin, 100 µg thiamine hydrochloride and 0.5 µg cobalamin per liter) at −80 °C.
Techniques: Concentration Assay
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