scc 9 cells  (ATCC)


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    ATCC scc 9 cells
    Scc 9 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scc 9 cells  (ATCC)


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    ATCC scc 9 cells
    Establishment of TCONS_00006091 signaling pathways (*P value < 0.05 vs. miR control group). ( A ) Bioinformatic analysis and luciferase assay on the relationship between TCONS_00006091 and miR-153 in HSC-3 and <t>SCC-9</t> cells; ( B ) Bioinformatic analysis and luciferase assay on the relationship between TCONS_00006091 and miR-370 in HSC-3 and SCC-9 cells; ( C ) Bioinformatic analysis and luciferase assay on the relationship between TCONS_00006091 and let-7g in HSC-3 and SCC-9 cells; ( D ) Bioinformatic analysis and luciferase assay on the relationship between miR-153 and SNAI1 mRNA in HSC-3 and SCC-9 cells; ( E ) Bioinformatic analysis and luciferase assay on the relationship between miR-370 and IRS mRNA in HSC-3 and SCC-9 cells; ( F ) Bioinformatic analysis and luciferase assay on the relationship between let-7g and HMGA2 in HSC-3 and SCC-9 cells.
    Scc 9 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Dysregulation of TCONS_00006091 contributes to the elevated risk of oral squamous cell carcinoma by upregulating SNAI1, IRS and HMGA2"

    Article Title: Dysregulation of TCONS_00006091 contributes to the elevated risk of oral squamous cell carcinoma by upregulating SNAI1, IRS and HMGA2

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-60310-4

    Establishment of TCONS_00006091 signaling pathways (*P value < 0.05 vs. miR control group). ( A ) Bioinformatic analysis and luciferase assay on the relationship between TCONS_00006091 and miR-153 in HSC-3 and SCC-9 cells; ( B ) Bioinformatic analysis and luciferase assay on the relationship between TCONS_00006091 and miR-370 in HSC-3 and SCC-9 cells; ( C ) Bioinformatic analysis and luciferase assay on the relationship between TCONS_00006091 and let-7g in HSC-3 and SCC-9 cells; ( D ) Bioinformatic analysis and luciferase assay on the relationship between miR-153 and SNAI1 mRNA in HSC-3 and SCC-9 cells; ( E ) Bioinformatic analysis and luciferase assay on the relationship between miR-370 and IRS mRNA in HSC-3 and SCC-9 cells; ( F ) Bioinformatic analysis and luciferase assay on the relationship between let-7g and HMGA2 in HSC-3 and SCC-9 cells.
    Figure Legend Snippet: Establishment of TCONS_00006091 signaling pathways (*P value < 0.05 vs. miR control group). ( A ) Bioinformatic analysis and luciferase assay on the relationship between TCONS_00006091 and miR-153 in HSC-3 and SCC-9 cells; ( B ) Bioinformatic analysis and luciferase assay on the relationship between TCONS_00006091 and miR-370 in HSC-3 and SCC-9 cells; ( C ) Bioinformatic analysis and luciferase assay on the relationship between TCONS_00006091 and let-7g in HSC-3 and SCC-9 cells; ( D ) Bioinformatic analysis and luciferase assay on the relationship between miR-153 and SNAI1 mRNA in HSC-3 and SCC-9 cells; ( E ) Bioinformatic analysis and luciferase assay on the relationship between miR-370 and IRS mRNA in HSC-3 and SCC-9 cells; ( F ) Bioinformatic analysis and luciferase assay on the relationship between let-7g and HMGA2 in HSC-3 and SCC-9 cells.

    Techniques Used: Luciferase

    Over-expression of TCONS_00006091 regulated the gene and protein expressions, as well as the proliferation and apoptosis of HSC-3 and SCC-9 cells (* P value < 0.05 vs. NC group). ( A ) Relative expression of TCONS_00006091 in p-TCONS_00006091 and NC groups; ( B ) Relative expression of miR-153 in p-TCONS_00006091 and NC groups; ( C ) Relative expression of miR-370 in p-TCONS_00006091 and NC groups; ( D ) Relative expression of let-7g in p-TCONS_00006091 and NC groups; ( E ) Relative mRNA expression of SNAI1 in p-TCONS_00006091 and NC groups; ( F ) Western blot results of SNAI1 protein in p-TCONS_00006091 and NC groups; ( G ) Relative protein expression of SNAI1 in p-TCONS_00006091 and NC groups; ( H ) Relative mRNA expression of IRS in p-TCONS_00006091 and NC groups; ( I ) Western blot results of IRS protein in p-TCONS_00006091 and NC groups; ( J ) Relative protein expression of IRS in p-TCONS_00006091 and NC groups; ( K ) Relative mRNA expression of HMGA2 in p-TCONS_00006091 and NC groups; ( L ) Western blot results of HMGA2 protein in p-TCONS_00006091 and NC groups; ( M ) Relative protein expression of HMGA2 in p-TCONS_00006091 and NC groups; ( N ) The number of viable cells in p-TCONS_00006091 and NC groups detected by MTT assays; ( O ) The cell apoptosis rate in p-TCONS_00006091 and NC groups detected by FCM assays.
    Figure Legend Snippet: Over-expression of TCONS_00006091 regulated the gene and protein expressions, as well as the proliferation and apoptosis of HSC-3 and SCC-9 cells (* P value < 0.05 vs. NC group). ( A ) Relative expression of TCONS_00006091 in p-TCONS_00006091 and NC groups; ( B ) Relative expression of miR-153 in p-TCONS_00006091 and NC groups; ( C ) Relative expression of miR-370 in p-TCONS_00006091 and NC groups; ( D ) Relative expression of let-7g in p-TCONS_00006091 and NC groups; ( E ) Relative mRNA expression of SNAI1 in p-TCONS_00006091 and NC groups; ( F ) Western blot results of SNAI1 protein in p-TCONS_00006091 and NC groups; ( G ) Relative protein expression of SNAI1 in p-TCONS_00006091 and NC groups; ( H ) Relative mRNA expression of IRS in p-TCONS_00006091 and NC groups; ( I ) Western blot results of IRS protein in p-TCONS_00006091 and NC groups; ( J ) Relative protein expression of IRS in p-TCONS_00006091 and NC groups; ( K ) Relative mRNA expression of HMGA2 in p-TCONS_00006091 and NC groups; ( L ) Western blot results of HMGA2 protein in p-TCONS_00006091 and NC groups; ( M ) Relative protein expression of HMGA2 in p-TCONS_00006091 and NC groups; ( N ) The number of viable cells in p-TCONS_00006091 and NC groups detected by MTT assays; ( O ) The cell apoptosis rate in p-TCONS_00006091 and NC groups detected by FCM assays.

    Techniques Used: Over Expression, Expressing, Western Blot

    Knockdown of TCONS_00006091 regulated the gene and protein expressions, as well as the proliferation and apoptosis of HSC-3 and SCC-9 cells (* P value < 0.05 vs. NC group). ( A ) Relative expression of TCONS_00006091 in p-TCONS_00006091 shRNA and NC shRNA groups; ( B ) Relative expression of miR-153 in p-TCONS_00006091 shRNA and NC shRNA groups; ( C ) Relative expression of miR-370 in p-TCONS_00006091 shRNA and NC shRNA groups; ( D ) Relative expression of let-7g in p-TCONS_00006091 shRNA and NC shRNA groups; ( E ) Relative mRNA expression of SNAI1 in p-TCONS_00006091 shRNA and NC shRNA groups; ( F ) Western blot results of SNAI1 protein in p-TCONS_00006091 shRNA and NC shRNA groups; ( G ) Relative protein expression of SNAI1 in p-TCONS_00006091 shRNA and NC shRNA groups; ( H ) Relative mRNA expression of IRS in p-TCONS_00006091 shRNA and NC shRNA groups; ( I ) Western blot results of IRS protein in p-TCONS_00006091 shRNA and NC shRNA groups; ( J ) Relative protein expression of IRS in p-TCONS_00006091 shRNA and NC shRNA groups; ( K ) Relative mRNA expression of HMGA2 in p-TCONS_00006091 shRNA and NC shRNA groups; ( L ) Western blot results of HMGA2 protein in p-TCONS_00006091 shRNA and NC shRNA groups; ( M ) Relative protein expression of HMGA2 in p-TCONS_00006091 shRNA and NC shRNA groups; ( N ) The number of viable cells in p-TCONS_00006091 shRNA and NC shRNA groups detected by MTT assays; ( O ) The cell apoptosis rate in p-TCONS_00006091 shRNA and NC shRNA groups detected by FCM assays.
    Figure Legend Snippet: Knockdown of TCONS_00006091 regulated the gene and protein expressions, as well as the proliferation and apoptosis of HSC-3 and SCC-9 cells (* P value < 0.05 vs. NC group). ( A ) Relative expression of TCONS_00006091 in p-TCONS_00006091 shRNA and NC shRNA groups; ( B ) Relative expression of miR-153 in p-TCONS_00006091 shRNA and NC shRNA groups; ( C ) Relative expression of miR-370 in p-TCONS_00006091 shRNA and NC shRNA groups; ( D ) Relative expression of let-7g in p-TCONS_00006091 shRNA and NC shRNA groups; ( E ) Relative mRNA expression of SNAI1 in p-TCONS_00006091 shRNA and NC shRNA groups; ( F ) Western blot results of SNAI1 protein in p-TCONS_00006091 shRNA and NC shRNA groups; ( G ) Relative protein expression of SNAI1 in p-TCONS_00006091 shRNA and NC shRNA groups; ( H ) Relative mRNA expression of IRS in p-TCONS_00006091 shRNA and NC shRNA groups; ( I ) Western blot results of IRS protein in p-TCONS_00006091 shRNA and NC shRNA groups; ( J ) Relative protein expression of IRS in p-TCONS_00006091 shRNA and NC shRNA groups; ( K ) Relative mRNA expression of HMGA2 in p-TCONS_00006091 shRNA and NC shRNA groups; ( L ) Western blot results of HMGA2 protein in p-TCONS_00006091 shRNA and NC shRNA groups; ( M ) Relative protein expression of HMGA2 in p-TCONS_00006091 shRNA and NC shRNA groups; ( N ) The number of viable cells in p-TCONS_00006091 shRNA and NC shRNA groups detected by MTT assays; ( O ) The cell apoptosis rate in p-TCONS_00006091 shRNA and NC shRNA groups detected by FCM assays.

    Techniques Used: Expressing, shRNA, Western Blot

    scc 25  (ATCC)


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    ATCC scc 25
    Scc 25, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scc 25  (ATCC)


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    ATCC scc 25
    Effects of interleukin 33 (IL-33) in <t>SCC25</t> and Detroit 562 cells. ( a ) Relative wound density curve of SCC25 cells for the IncuCyte analysis over 24 h. Representative images of scratch assays show scratches immediately after they were made (0 h) and after 24 h in the presence of IL-33 (right panels) versus positive control medium (left panels). The scale bars represent 300 μm. ( b ) Proliferation curve for SCC25 cells in the presence of IL-33. Representative images of the proliferation assay. ( c ) Relative wound density curve of Detroit 562 cells for the IncuCyte analysis over 24 h. Representative images of scratch assays show scratches immediately after they were made (0 h) and after 24 h in the presence of IL-33 (right panels) versus positive control medium (left panels). The scale bars represent 300 μm. ( d ) Proliferation curve for Detroit 562 cells in the presence of IL-33. Representative images of the proliferation assay. The data are shown as the mean ± standard error of the mean (SEM) from a single experiment and are representative of at least three experiments. ( e ) Transwell invasion assay for SCC25 cells at 48 h after incubation with IL-33 (10, 50 and 100 ng/ml). The data are shown as the mean ± SEM of cells counted in five representative microscopic fields per membrane and analysed using the ImageJ software. Representative images of invasion assay from two experiments. ( f ) Relative expression of EPCAM , SOX2 , NANOG , MYC , EZH2 , CHGA , AURKA , MYCN and SYP in SCC25 cells determined with quantitative polymerase chain reaction (mean ± SEM, n = 3). ( g ) Relative expression of EPCAM , SOX2 , NANOG , MYC , EZH2 , CHGA , AURKA , MYCN and SYP in Detroit 562 cells determined with quantitative polymerase chain reaction (mean ± SEM, n = 3). * P < 0.05, (** P < 0.01), *** P < 0.001 and **** P < 0.0001
    Scc 25, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Interleukin 33 supports squamous cell carcinoma growth via a dual effect on tumour proliferation, migration and invasion, and T cell activation"

    Article Title: Interleukin 33 supports squamous cell carcinoma growth via a dual effect on tumour proliferation, migration and invasion, and T cell activation

    Journal: Cancer Immunology, Immunotherapy : CII

    doi: 10.1007/s00262-024-03676-8

    Effects of interleukin 33 (IL-33) in SCC25 and Detroit 562 cells. ( a ) Relative wound density curve of SCC25 cells for the IncuCyte analysis over 24 h. Representative images of scratch assays show scratches immediately after they were made (0 h) and after 24 h in the presence of IL-33 (right panels) versus positive control medium (left panels). The scale bars represent 300 μm. ( b ) Proliferation curve for SCC25 cells in the presence of IL-33. Representative images of the proliferation assay. ( c ) Relative wound density curve of Detroit 562 cells for the IncuCyte analysis over 24 h. Representative images of scratch assays show scratches immediately after they were made (0 h) and after 24 h in the presence of IL-33 (right panels) versus positive control medium (left panels). The scale bars represent 300 μm. ( d ) Proliferation curve for Detroit 562 cells in the presence of IL-33. Representative images of the proliferation assay. The data are shown as the mean ± standard error of the mean (SEM) from a single experiment and are representative of at least three experiments. ( e ) Transwell invasion assay for SCC25 cells at 48 h after incubation with IL-33 (10, 50 and 100 ng/ml). The data are shown as the mean ± SEM of cells counted in five representative microscopic fields per membrane and analysed using the ImageJ software. Representative images of invasion assay from two experiments. ( f ) Relative expression of EPCAM , SOX2 , NANOG , MYC , EZH2 , CHGA , AURKA , MYCN and SYP in SCC25 cells determined with quantitative polymerase chain reaction (mean ± SEM, n = 3). ( g ) Relative expression of EPCAM , SOX2 , NANOG , MYC , EZH2 , CHGA , AURKA , MYCN and SYP in Detroit 562 cells determined with quantitative polymerase chain reaction (mean ± SEM, n = 3). * P < 0.05, (** P < 0.01), *** P < 0.001 and **** P < 0.0001
    Figure Legend Snippet: Effects of interleukin 33 (IL-33) in SCC25 and Detroit 562 cells. ( a ) Relative wound density curve of SCC25 cells for the IncuCyte analysis over 24 h. Representative images of scratch assays show scratches immediately after they were made (0 h) and after 24 h in the presence of IL-33 (right panels) versus positive control medium (left panels). The scale bars represent 300 μm. ( b ) Proliferation curve for SCC25 cells in the presence of IL-33. Representative images of the proliferation assay. ( c ) Relative wound density curve of Detroit 562 cells for the IncuCyte analysis over 24 h. Representative images of scratch assays show scratches immediately after they were made (0 h) and after 24 h in the presence of IL-33 (right panels) versus positive control medium (left panels). The scale bars represent 300 μm. ( d ) Proliferation curve for Detroit 562 cells in the presence of IL-33. Representative images of the proliferation assay. The data are shown as the mean ± standard error of the mean (SEM) from a single experiment and are representative of at least three experiments. ( e ) Transwell invasion assay for SCC25 cells at 48 h after incubation with IL-33 (10, 50 and 100 ng/ml). The data are shown as the mean ± SEM of cells counted in five representative microscopic fields per membrane and analysed using the ImageJ software. Representative images of invasion assay from two experiments. ( f ) Relative expression of EPCAM , SOX2 , NANOG , MYC , EZH2 , CHGA , AURKA , MYCN and SYP in SCC25 cells determined with quantitative polymerase chain reaction (mean ± SEM, n = 3). ( g ) Relative expression of EPCAM , SOX2 , NANOG , MYC , EZH2 , CHGA , AURKA , MYCN and SYP in Detroit 562 cells determined with quantitative polymerase chain reaction (mean ± SEM, n = 3). * P < 0.05, (** P < 0.01), *** P < 0.001 and **** P < 0.0001

    Techniques Used: Positive Control, Proliferation Assay, Transwell Invasion Assay, Incubation, Membrane, Software, Invasion Assay, Expressing, Real-time Polymerase Chain Reaction

    human squamous cell carcinoma scc  (ATCC)


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    ATCC human squamous cell carcinoma scc
    Antitumor effect of ARN21934 tetrahydroquinazoline derivative on <t>SCC-25</t> cells. ( A ) Cell viability was measured at 24 h, 48 h and 72 h after treatment with ARN21934 for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean + SD of two biological specimen, each with three experimental replicates. Statistical differences were calculated with two-way ANOVA (Tukey’s multiple comparison test; **** p < 0.0001. ( B ) ARN21934 compound was tested on Human Dermal Fibroblast (nHDF) cells. Cell viability was measured at 24 h, 48 h and 72 h after treatment for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean ± SD calculated as propagation error of one biological replicate. ( C ) Cells growth inhibition observed within 72 h after treatment with a dose of ARN21934 below the IC50 value (0.8 μM) with respect to the control. Significant proliferation inhibition of ARN21934-treated cells was observed at 72 h with respect to the control. Statistical differences were calculated though the Sidàk’s multiple comparisons test, * p < 0.01. ( D ) Spheroids were treated with 20-fold higher doses than 2D cells for 2 h at 37 °C. Data were normalized over the viability of untreated spheroids and reported as mean + SD of three biological replicates. Statistical differences were calculated with Student’s t-test, ** p < 0.01.
    Human Squamous Cell Carcinoma Scc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human squamous cell carcinoma scc - by Bioz Stars, 2024-05
    86/100 stars

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    1) Product Images from "Tumor growth-arrest effect of tetrahydroquinazoline-derivative human topoisomerase II-alpha inhibitor in HPV-negative head and neck squamous cell carcinoma"

    Article Title: Tumor growth-arrest effect of tetrahydroquinazoline-derivative human topoisomerase II-alpha inhibitor in HPV-negative head and neck squamous cell carcinoma

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-59592-5

    Antitumor effect of ARN21934 tetrahydroquinazoline derivative on SCC-25 cells. ( A ) Cell viability was measured at 24 h, 48 h and 72 h after treatment with ARN21934 for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean + SD of two biological specimen, each with three experimental replicates. Statistical differences were calculated with two-way ANOVA (Tukey’s multiple comparison test; **** p < 0.0001. ( B ) ARN21934 compound was tested on Human Dermal Fibroblast (nHDF) cells. Cell viability was measured at 24 h, 48 h and 72 h after treatment for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean ± SD calculated as propagation error of one biological replicate. ( C ) Cells growth inhibition observed within 72 h after treatment with a dose of ARN21934 below the IC50 value (0.8 μM) with respect to the control. Significant proliferation inhibition of ARN21934-treated cells was observed at 72 h with respect to the control. Statistical differences were calculated though the Sidàk’s multiple comparisons test, * p < 0.01. ( D ) Spheroids were treated with 20-fold higher doses than 2D cells for 2 h at 37 °C. Data were normalized over the viability of untreated spheroids and reported as mean + SD of three biological replicates. Statistical differences were calculated with Student’s t-test, ** p < 0.01.
    Figure Legend Snippet: Antitumor effect of ARN21934 tetrahydroquinazoline derivative on SCC-25 cells. ( A ) Cell viability was measured at 24 h, 48 h and 72 h after treatment with ARN21934 for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean + SD of two biological specimen, each with three experimental replicates. Statistical differences were calculated with two-way ANOVA (Tukey’s multiple comparison test; **** p < 0.0001. ( B ) ARN21934 compound was tested on Human Dermal Fibroblast (nHDF) cells. Cell viability was measured at 24 h, 48 h and 72 h after treatment for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean ± SD calculated as propagation error of one biological replicate. ( C ) Cells growth inhibition observed within 72 h after treatment with a dose of ARN21934 below the IC50 value (0.8 μM) with respect to the control. Significant proliferation inhibition of ARN21934-treated cells was observed at 72 h with respect to the control. Statistical differences were calculated though the Sidàk’s multiple comparisons test, * p < 0.01. ( D ) Spheroids were treated with 20-fold higher doses than 2D cells for 2 h at 37 °C. Data were normalized over the viability of untreated spheroids and reported as mean + SD of three biological replicates. Statistical differences were calculated with Student’s t-test, ** p < 0.01.

    Techniques Used: Comparison, Inhibition

    human keratinocyte cell line scc  (ATCC)


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    ATCC human keratinocyte cell line scc
    Human Keratinocyte Cell Line Scc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human keratinocyte cell line scc  (ATCC)


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    ATCC human keratinocyte cell line scc
    Human Keratinocyte Cell Line Scc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human keratinocyte cell line scc/product/ATCC
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    scc mec types  (ATCC)


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    ATCC scc mec types
    Pulsotypes of S. aureus digested by SmaI and their corresponding molecular typing results including MLST, <t>SCC</t> <t>mec</t> , and spa types. Fifteen pulsotypes (from A to M) were determined in 42 S. aureus isolates, including 26 from patients with mastitis, 15 from the environmental screening, and one control strain (M003) from a patient with a buttock abscess. NCTC 8325, S. aureus reference strain used as a marker.
    Scc Mec Types, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Puerperal mastitis caused by limited community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones"

    Article Title: Puerperal mastitis caused by limited community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones

    Journal: Frontiers in Medicine

    doi: 10.3389/fmed.2024.1378207

    Pulsotypes of S. aureus digested by SmaI and their corresponding molecular typing results including MLST, SCC mec , and spa types. Fifteen pulsotypes (from A to M) were determined in 42 S. aureus isolates, including 26 from patients with mastitis, 15 from the environmental screening, and one control strain (M003) from a patient with a buttock abscess. NCTC 8325, S. aureus reference strain used as a marker.
    Figure Legend Snippet: Pulsotypes of S. aureus digested by SmaI and their corresponding molecular typing results including MLST, SCC mec , and spa types. Fifteen pulsotypes (from A to M) were determined in 42 S. aureus isolates, including 26 from patients with mastitis, 15 from the environmental screening, and one control strain (M003) from a patient with a buttock abscess. NCTC 8325, S. aureus reference strain used as a marker.

    Techniques Used: Marker

    scc 15 cells  (ATCC)


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    ATCC scc 15 cells
    Scc 15 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scc 9  (ATCC)


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    ATCC scc 9
    Scc 9, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC scc 9 cells
    Scc 9 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scc 25  (ATCC)
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    ATCC scc 25
    Scc 25, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human squamous cell carcinoma scc
    Antitumor effect of ARN21934 tetrahydroquinazoline derivative on <t>SCC-25</t> cells. ( A ) Cell viability was measured at 24 h, 48 h and 72 h after treatment with ARN21934 for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean + SD of two biological specimen, each with three experimental replicates. Statistical differences were calculated with two-way ANOVA (Tukey’s multiple comparison test; **** p < 0.0001. ( B ) ARN21934 compound was tested on Human Dermal Fibroblast (nHDF) cells. Cell viability was measured at 24 h, 48 h and 72 h after treatment for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean ± SD calculated as propagation error of one biological replicate. ( C ) Cells growth inhibition observed within 72 h after treatment with a dose of ARN21934 below the IC50 value (0.8 μM) with respect to the control. Significant proliferation inhibition of ARN21934-treated cells was observed at 72 h with respect to the control. Statistical differences were calculated though the Sidàk’s multiple comparisons test, * p < 0.01. ( D ) Spheroids were treated with 20-fold higher doses than 2D cells for 2 h at 37 °C. Data were normalized over the viability of untreated spheroids and reported as mean + SD of three biological replicates. Statistical differences were calculated with Student’s t-test, ** p < 0.01.
    Human Squamous Cell Carcinoma Scc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human keratinocyte cell line scc
    Antitumor effect of ARN21934 tetrahydroquinazoline derivative on <t>SCC-25</t> cells. ( A ) Cell viability was measured at 24 h, 48 h and 72 h after treatment with ARN21934 for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean + SD of two biological specimen, each with three experimental replicates. Statistical differences were calculated with two-way ANOVA (Tukey’s multiple comparison test; **** p < 0.0001. ( B ) ARN21934 compound was tested on Human Dermal Fibroblast (nHDF) cells. Cell viability was measured at 24 h, 48 h and 72 h after treatment for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean ± SD calculated as propagation error of one biological replicate. ( C ) Cells growth inhibition observed within 72 h after treatment with a dose of ARN21934 below the IC50 value (0.8 μM) with respect to the control. Significant proliferation inhibition of ARN21934-treated cells was observed at 72 h with respect to the control. Statistical differences were calculated though the Sidàk’s multiple comparisons test, * p < 0.01. ( D ) Spheroids were treated with 20-fold higher doses than 2D cells for 2 h at 37 °C. Data were normalized over the viability of untreated spheroids and reported as mean + SD of three biological replicates. Statistical differences were calculated with Student’s t-test, ** p < 0.01.
    Human Keratinocyte Cell Line Scc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC scc mec types
    Pulsotypes of S. aureus digested by SmaI and their corresponding molecular typing results including MLST, <t>SCC</t> <t>mec</t> , and spa types. Fifteen pulsotypes (from A to M) were determined in 42 S. aureus isolates, including 26 from patients with mastitis, 15 from the environmental screening, and one control strain (M003) from a patient with a buttock abscess. NCTC 8325, S. aureus reference strain used as a marker.
    Scc Mec Types, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC scc 15 cells
    Pulsotypes of S. aureus digested by SmaI and their corresponding molecular typing results including MLST, <t>SCC</t> <t>mec</t> , and spa types. Fifteen pulsotypes (from A to M) were determined in 42 S. aureus isolates, including 26 from patients with mastitis, 15 from the environmental screening, and one control strain (M003) from a patient with a buttock abscess. NCTC 8325, S. aureus reference strain used as a marker.
    Scc 15 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scc 9  (ATCC)
    86
    ATCC scc 9
    Pulsotypes of S. aureus digested by SmaI and their corresponding molecular typing results including MLST, <t>SCC</t> <t>mec</t> , and spa types. Fifteen pulsotypes (from A to M) were determined in 42 S. aureus isolates, including 26 from patients with mastitis, 15 from the environmental screening, and one control strain (M003) from a patient with a buttock abscess. NCTC 8325, S. aureus reference strain used as a marker.
    Scc 9, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/scc 9/product/ATCC
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    Antitumor effect of ARN21934 tetrahydroquinazoline derivative on SCC-25 cells. ( A ) Cell viability was measured at 24 h, 48 h and 72 h after treatment with ARN21934 for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean + SD of two biological specimen, each with three experimental replicates. Statistical differences were calculated with two-way ANOVA (Tukey’s multiple comparison test; **** p < 0.0001. ( B ) ARN21934 compound was tested on Human Dermal Fibroblast (nHDF) cells. Cell viability was measured at 24 h, 48 h and 72 h after treatment for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean ± SD calculated as propagation error of one biological replicate. ( C ) Cells growth inhibition observed within 72 h after treatment with a dose of ARN21934 below the IC50 value (0.8 μM) with respect to the control. Significant proliferation inhibition of ARN21934-treated cells was observed at 72 h with respect to the control. Statistical differences were calculated though the Sidàk’s multiple comparisons test, * p < 0.01. ( D ) Spheroids were treated with 20-fold higher doses than 2D cells for 2 h at 37 °C. Data were normalized over the viability of untreated spheroids and reported as mean + SD of three biological replicates. Statistical differences were calculated with Student’s t-test, ** p < 0.01.

    Journal: Scientific Reports

    Article Title: Tumor growth-arrest effect of tetrahydroquinazoline-derivative human topoisomerase II-alpha inhibitor in HPV-negative head and neck squamous cell carcinoma

    doi: 10.1038/s41598-024-59592-5

    Figure Lengend Snippet: Antitumor effect of ARN21934 tetrahydroquinazoline derivative on SCC-25 cells. ( A ) Cell viability was measured at 24 h, 48 h and 72 h after treatment with ARN21934 for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean + SD of two biological specimen, each with three experimental replicates. Statistical differences were calculated with two-way ANOVA (Tukey’s multiple comparison test; **** p < 0.0001. ( B ) ARN21934 compound was tested on Human Dermal Fibroblast (nHDF) cells. Cell viability was measured at 24 h, 48 h and 72 h after treatment for 2 h at 37 °C. Data were normalized over the viability of control cells treated with medium. Data are reported as mean ± SD calculated as propagation error of one biological replicate. ( C ) Cells growth inhibition observed within 72 h after treatment with a dose of ARN21934 below the IC50 value (0.8 μM) with respect to the control. Significant proliferation inhibition of ARN21934-treated cells was observed at 72 h with respect to the control. Statistical differences were calculated though the Sidàk’s multiple comparisons test, * p < 0.01. ( D ) Spheroids were treated with 20-fold higher doses than 2D cells for 2 h at 37 °C. Data were normalized over the viability of untreated spheroids and reported as mean + SD of three biological replicates. Statistical differences were calculated with Student’s t-test, ** p < 0.01.

    Article Snippet: Human squamous cell carcinoma SCC-25 was purchased from the American Type Culture Collection (ATCC).

    Techniques: Comparison, Inhibition

    Pulsotypes of S. aureus digested by SmaI and their corresponding molecular typing results including MLST, SCC mec , and spa types. Fifteen pulsotypes (from A to M) were determined in 42 S. aureus isolates, including 26 from patients with mastitis, 15 from the environmental screening, and one control strain (M003) from a patient with a buttock abscess. NCTC 8325, S. aureus reference strain used as a marker.

    Journal: Frontiers in Medicine

    Article Title: Puerperal mastitis caused by limited community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) clones

    doi: 10.3389/fmed.2024.1378207

    Figure Lengend Snippet: Pulsotypes of S. aureus digested by SmaI and their corresponding molecular typing results including MLST, SCC mec , and spa types. Fifteen pulsotypes (from A to M) were determined in 42 S. aureus isolates, including 26 from patients with mastitis, 15 from the environmental screening, and one control strain (M003) from a patient with a buttock abscess. NCTC 8325, S. aureus reference strain used as a marker.

    Article Snippet: SCC mec types I through V in MRSA strains were identified by comparing the M-PCR banding patterns of the isolates with those of the reference strains: ATCC 10442 (SCC mec type I), N315 (SCC mec type II), 85/2082 (SCC mec type III), MW2 (SCC mec type IVa), WIS (SCC mec type V), and TSGH-17 (SCC mec type V T ).

    Techniques: Marker