Structured Review

Agilent technologies scanner model g2565ca
Scanner Model G2565ca, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/scanner model g2565ca/product/Agilent technologies
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
scanner model g2565ca - by Bioz Stars, 2020-04
90/100 stars

Related Products / Commonly Used Together

dna microarrays

Images

Related Articles

Amplification:

Article Title: Myeloid cell expressed proprotein convertase FURIN attenuates inflammation
Article Snippet: Initial and purified RNA contents along with dyed and amplified cRNA contents were measured with a NanoDrop ND-2000 (Thermo Fisher Scientific, Massachusetts, USA). .. The chips were scanned using an Agilent Technologies Scanner model G2565CA using the scan profile AgilentG3_GX_2Color.

Article Title: Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen
Article Snippet: 600 ng of total RNA was amplified and labelled with Cy3 using the Quick Amp Labeling kit (Agilent USA). .. The DNA microarrays were scanned using an Agilent Technologies’ Scanner model G2565CA.

Labeling:

Article Title: Myeloid cell expressed proprotein convertase FURIN attenuates inflammation
Article Snippet: 300ng of Cy-3 and 300ng of Cy-5 labeled sample were hybridized together on an Agilent Mouse Chip 8×60K (Design ID 028005) (Agilent Technologies, California, USA) overnight at 65°C using the Gene Expression Hybridization kit. .. The chips were scanned using an Agilent Technologies Scanner model G2565CA using the scan profile AgilentG3_GX_2Color.

Article Title: Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen
Article Snippet: 600 ng of total RNA was amplified and labelled with Cy3 using the Quick Amp Labeling kit (Agilent USA). .. The DNA microarrays were scanned using an Agilent Technologies’ Scanner model G2565CA.

Purification:

Article Title: Myeloid cell expressed proprotein convertase FURIN attenuates inflammation
Article Snippet: Initial and purified RNA contents along with dyed and amplified cRNA contents were measured with a NanoDrop ND-2000 (Thermo Fisher Scientific, Massachusetts, USA). .. The chips were scanned using an Agilent Technologies Scanner model G2565CA using the scan profile AgilentG3_GX_2Color.

Microarray:

Article Title: Myeloid cell expressed proprotein convertase FURIN attenuates inflammation
Article Snippet: Paragraph title: Microarray data analysis ... The chips were scanned using an Agilent Technologies Scanner model G2565CA using the scan profile AgilentG3_GX_2Color.

Article Title: Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen
Article Snippet: Paragraph title: Microarray hybridization ... The DNA microarrays were scanned using an Agilent Technologies’ Scanner model G2565CA.

Expressing:

Article Title: Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen
Article Snippet: The arrays were washed using the Gene Expression Wash Pack and Stabilization and Drying solutions also provided in the custom DNA microarray gene expression profiling kit. .. The DNA microarrays were scanned using an Agilent Technologies’ Scanner model G2565CA.

Hybridization:

Article Title: Myeloid cell expressed proprotein convertase FURIN attenuates inflammation
Article Snippet: 300ng of Cy-3 and 300ng of Cy-5 labeled sample were hybridized together on an Agilent Mouse Chip 8×60K (Design ID 028005) (Agilent Technologies, California, USA) overnight at 65°C using the Gene Expression Hybridization kit. .. The chips were scanned using an Agilent Technologies Scanner model G2565CA using the scan profile AgilentG3_GX_2Color.

Article Title: Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen
Article Snippet: Paragraph title: Microarray hybridization ... The DNA microarrays were scanned using an Agilent Technologies’ Scanner model G2565CA.

Chromatin Immunoprecipitation:

Article Title: Myeloid cell expressed proprotein convertase FURIN attenuates inflammation
Article Snippet: 300ng of Cy-3 and 300ng of Cy-5 labeled sample were hybridized together on an Agilent Mouse Chip 8×60K (Design ID 028005) (Agilent Technologies, California, USA) overnight at 65°C using the Gene Expression Hybridization kit. .. The chips were scanned using an Agilent Technologies Scanner model G2565CA using the scan profile AgilentG3_GX_2Color.

Derivative Assay:

Article Title: Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen
Article Snippet: The DNA microarrays were scanned using an Agilent Technologies’ Scanner model G2565CA. .. The expression data was derived from the image files using Agilent’s Feature Extraction software, version 10.5.1.1, using grid 024161_D_F_20090605, protocol GE1_105_Dec08 and QC metric set GE1_QCMT_Dec08.

Software:

Article Title: Myeloid cell expressed proprotein convertase FURIN attenuates inflammation
Article Snippet: The chips were scanned using an Agilent Technologies Scanner model G2565CA using the scan profile AgilentG3_GX_2Color. .. Scan results were converted into numerical data by the Agilent Feature Extraction software version 10.7.3.

Article Title: Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen
Article Snippet: The DNA microarrays were scanned using an Agilent Technologies’ Scanner model G2565CA. .. The expression data was derived from the image files using Agilent’s Feature Extraction software, version 10.5.1.1, using grid 024161_D_F_20090605, protocol GE1_105_Dec08 and QC metric set GE1_QCMT_Dec08.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Agilent technologies microarray scanner g model g2565ba
    CD6 co-stimulation with activated leucocyte-cell adhesion molecule (ALCAM) induces a distinct gene transcription profile which is amplified further by interleukin (IL)-2. Difference in gene expression profile between 3 × 10 4 freshly isolated human peripheral blood mononuclear cells (PBMC) stimulated for 72 h with the immobilized anti-CD3 monoclonal antibody (mAb) (0·25 µg/ml) alone (lane 1), with ALCAM-Fc (1 µg/ml) (lane 2), interleukin (IL)-2 (2·5 ng/ml) (lane 4) or both (lane 3) or compared to unstimulated cells (lane 5). (a) Clustering of 15 000 genes or (b) 60 selected genes regulated differentially relative to the unstimulated cells are depicted. (c) Selected genes expression in CD3-activated cells incubated with a soluble CD6 membrane-distal domain specific humanized immunoglobulin (Ig)G1 non-depleting mAb (lane 7) or an irrelevant isotype control mAb (lane 6) at 10 µg/ml, compared to unstimulated cells (lane 8). (d) Colour bar ranging from 0 to 15-fold change relative to control. RNA was extracted by using Trizol reagent protocol. The labelled RNA was hybridized onto the human gene expression array 8 × 15 K. Hybridization was carried out in Agilent's Surehyb Chambers at 65°C for 16 h. The hybridized slides were washed and scanned using Agilent <t>microarray</t> <t>scanner</t> G Model <t>G2565BA</t> at 5 micron resolution.
    Microarray Scanner G Model G2565ba, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner g model g2565ba/product/Agilent technologies
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    microarray scanner g model g2565ba - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    99
    Agilent technologies dna microarray scanner
    Double-round T7 amplification results in reduced technical variation compared to LM-PCR. Histograms of averaged binding ratios of four hybridizations with double-T7 ( A ) or LM-PCR amplified samples ( B ) across all <t>DNA</t> <t>microarray</t> probes. ( C ) Overview plot of chromosome 7 showing locations of array probes (black) and genes (green). Graphs of binding ratios of input versus ChIP show the background and binding signals for double-round T7 amplification (double-T7) (top) and LM-PCR (bottom). ( D ) Enlargement of indicated regions in (C).
    Dna Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray scanner/product/Agilent technologies
    Average 99 stars, based on 1473 article reviews
    Price from $9.99 to $1999.99
    dna microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies microarray scanner
    Network based analysis of genes affected by CD40:Fc treatment. <t>Microarray</t> analysis was performed on SG of CD40:Fc treated and control mice (n = 4 each, treatment at 10 weeks, end of study at 20 weeks) and gene changes greater than 2 fold were used in the analysis and compared for biological functional pathways or biomarkers by an Ingenuity Pathway analysis (IPA). ( A ) The pathway of NF-κB and TGF-β was affected in CD40:Fc treated mice. Green colored shapes indicates upregulated genes, red colored shapes downregulated; gray colored shapes genes in the pathway that were not differentially expressed. Color intensity reflects the degree of differential expression. Triangles represent phosphatases; horizontal diamond, peptidases; vertical diamond, enzymes; horizontal oval, transcription factors; vertical oval, transmembrane receptors; trapezoid, transporters; circle, other type of protein. Dotted lines indicate an indirect interaction; solid line, direct interaction, solid arrow head indicates “acts on”. ( B ) Metacore analysis of pathways linking differentially expressed genes to immune pathways. The differentially expressed genes are indicated along with the direction of change in expression. ( C ) Quantitative-PCR of selected genes shows agreement with microarray results. The results obtained using the microarray platform were validated by examining the correlation between the expression levels in the microarray and qPCR results obtained for a subset of genes.
    Microarray Scanner, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray scanner/product/Agilent technologies
    Average 99 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    microarray scanner - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    CD6 co-stimulation with activated leucocyte-cell adhesion molecule (ALCAM) induces a distinct gene transcription profile which is amplified further by interleukin (IL)-2. Difference in gene expression profile between 3 × 10 4 freshly isolated human peripheral blood mononuclear cells (PBMC) stimulated for 72 h with the immobilized anti-CD3 monoclonal antibody (mAb) (0·25 µg/ml) alone (lane 1), with ALCAM-Fc (1 µg/ml) (lane 2), interleukin (IL)-2 (2·5 ng/ml) (lane 4) or both (lane 3) or compared to unstimulated cells (lane 5). (a) Clustering of 15 000 genes or (b) 60 selected genes regulated differentially relative to the unstimulated cells are depicted. (c) Selected genes expression in CD3-activated cells incubated with a soluble CD6 membrane-distal domain specific humanized immunoglobulin (Ig)G1 non-depleting mAb (lane 7) or an irrelevant isotype control mAb (lane 6) at 10 µg/ml, compared to unstimulated cells (lane 8). (d) Colour bar ranging from 0 to 15-fold change relative to control. RNA was extracted by using Trizol reagent protocol. The labelled RNA was hybridized onto the human gene expression array 8 × 15 K. Hybridization was carried out in Agilent's Surehyb Chambers at 65°C for 16 h. The hybridized slides were washed and scanned using Agilent microarray scanner G Model G2565BA at 5 micron resolution.

    Journal: Clinical and Experimental Immunology

    Article Title: CD6 synergistic co-stimulation promoting proinflammatory response is modulated without interfering with the activated leucocyte cell adhesion molecule interaction

    doi: 10.1111/j.1365-2249.2010.04235.x

    Figure Lengend Snippet: CD6 co-stimulation with activated leucocyte-cell adhesion molecule (ALCAM) induces a distinct gene transcription profile which is amplified further by interleukin (IL)-2. Difference in gene expression profile between 3 × 10 4 freshly isolated human peripheral blood mononuclear cells (PBMC) stimulated for 72 h with the immobilized anti-CD3 monoclonal antibody (mAb) (0·25 µg/ml) alone (lane 1), with ALCAM-Fc (1 µg/ml) (lane 2), interleukin (IL)-2 (2·5 ng/ml) (lane 4) or both (lane 3) or compared to unstimulated cells (lane 5). (a) Clustering of 15 000 genes or (b) 60 selected genes regulated differentially relative to the unstimulated cells are depicted. (c) Selected genes expression in CD3-activated cells incubated with a soluble CD6 membrane-distal domain specific humanized immunoglobulin (Ig)G1 non-depleting mAb (lane 7) or an irrelevant isotype control mAb (lane 6) at 10 µg/ml, compared to unstimulated cells (lane 8). (d) Colour bar ranging from 0 to 15-fold change relative to control. RNA was extracted by using Trizol reagent protocol. The labelled RNA was hybridized onto the human gene expression array 8 × 15 K. Hybridization was carried out in Agilent's Surehyb Chambers at 65°C for 16 h. The hybridized slides were washed and scanned using Agilent microarray scanner G Model G2565BA at 5 micron resolution.

    Article Snippet: Hybridization was carried out in Agilent's Surehyb chambers at 65°C for 16 h. The hybridized slides were washed using Agilent gene expression wash buffers (part no. 5188-5327) and scanned using the Agilent microarray scanner G model G2565BA at 5 micron resolution.

    Techniques: Amplification, Expressing, Isolation, Incubation, Hybridization, Microarray

    Double-round T7 amplification results in reduced technical variation compared to LM-PCR. Histograms of averaged binding ratios of four hybridizations with double-T7 ( A ) or LM-PCR amplified samples ( B ) across all DNA microarray probes. ( C ) Overview plot of chromosome 7 showing locations of array probes (black) and genes (green). Graphs of binding ratios of input versus ChIP show the background and binding signals for double-round T7 amplification (double-T7) (top) and LM-PCR (bottom). ( D ) Enlargement of indicated regions in (C).

    Journal: Nucleic Acids Research

    Article Title: Improved genome-wide localization by ChIP-chip using double-round T7 RNA polymerase-based amplification

    doi: 10.1093/nar/gkm1144

    Figure Lengend Snippet: Double-round T7 amplification results in reduced technical variation compared to LM-PCR. Histograms of averaged binding ratios of four hybridizations with double-T7 ( A ) or LM-PCR amplified samples ( B ) across all DNA microarray probes. ( C ) Overview plot of chromosome 7 showing locations of array probes (black) and genes (green). Graphs of binding ratios of input versus ChIP show the background and binding signals for double-round T7 amplification (double-T7) (top) and LM-PCR (bottom). ( D ) Enlargement of indicated regions in (C).

    Article Snippet: Slides were scanned in an Agilent DNA Microarray Scanner (Model G2565BA, Agilent Technologies) and spot quantification was carried out using Imagene 4.0 (Biodiscovery).

    Techniques: Amplification, Polymerase Chain Reaction, Binding Assay, Microarray, Chromatin Immunoprecipitation

    Genome-wide transcriptomic data comparing Halobacterium sp. NRC-1 grown (A) aerobically ( y axis) versus anaerobically with arginine ( x axis) or (B) anaerobically with TMAO ( y axis) versus arginine ( x axis). DNA microarray data (fluorescence units) were

    Journal: Journal of Bacteriology

    Article Title: Genome-Wide Responses of the Model Archaeon Halobacterium sp. Strain NRC-1 to Oxygen Limitation

    doi: 10.1128/JB.01153-12

    Figure Lengend Snippet: Genome-wide transcriptomic data comparing Halobacterium sp. NRC-1 grown (A) aerobically ( y axis) versus anaerobically with arginine ( x axis) or (B) anaerobically with TMAO ( y axis) versus arginine ( x axis). DNA microarray data (fluorescence units) were

    Article Snippet: The labeled cDNA targets were combined and hybridized in duplicate for 17 h at 65°C in the dark, washed, and scanned for the Cy3 and Cy5 fluorescent signals on an Agilent DNA microarray scanner (model G2565BA).

    Techniques: Genome Wide, Microarray, Fluorescence

    Network based analysis of genes affected by CD40:Fc treatment. Microarray analysis was performed on SG of CD40:Fc treated and control mice (n = 4 each, treatment at 10 weeks, end of study at 20 weeks) and gene changes greater than 2 fold were used in the analysis and compared for biological functional pathways or biomarkers by an Ingenuity Pathway analysis (IPA). ( A ) The pathway of NF-κB and TGF-β was affected in CD40:Fc treated mice. Green colored shapes indicates upregulated genes, red colored shapes downregulated; gray colored shapes genes in the pathway that were not differentially expressed. Color intensity reflects the degree of differential expression. Triangles represent phosphatases; horizontal diamond, peptidases; vertical diamond, enzymes; horizontal oval, transcription factors; vertical oval, transmembrane receptors; trapezoid, transporters; circle, other type of protein. Dotted lines indicate an indirect interaction; solid line, direct interaction, solid arrow head indicates “acts on”. ( B ) Metacore analysis of pathways linking differentially expressed genes to immune pathways. The differentially expressed genes are indicated along with the direction of change in expression. ( C ) Quantitative-PCR of selected genes shows agreement with microarray results. The results obtained using the microarray platform were validated by examining the correlation between the expression levels in the microarray and qPCR results obtained for a subset of genes.

    Journal: PLoS ONE

    Article Title: Local Administration of Soluble CD40:Fc to the Salivary Glands of Non-Obese Diabetic Mice Does Not Ameliorate Autoimmune Inflammation

    doi: 10.1371/journal.pone.0051375

    Figure Lengend Snippet: Network based analysis of genes affected by CD40:Fc treatment. Microarray analysis was performed on SG of CD40:Fc treated and control mice (n = 4 each, treatment at 10 weeks, end of study at 20 weeks) and gene changes greater than 2 fold were used in the analysis and compared for biological functional pathways or biomarkers by an Ingenuity Pathway analysis (IPA). ( A ) The pathway of NF-κB and TGF-β was affected in CD40:Fc treated mice. Green colored shapes indicates upregulated genes, red colored shapes downregulated; gray colored shapes genes in the pathway that were not differentially expressed. Color intensity reflects the degree of differential expression. Triangles represent phosphatases; horizontal diamond, peptidases; vertical diamond, enzymes; horizontal oval, transcription factors; vertical oval, transmembrane receptors; trapezoid, transporters; circle, other type of protein. Dotted lines indicate an indirect interaction; solid line, direct interaction, solid arrow head indicates “acts on”. ( B ) Metacore analysis of pathways linking differentially expressed genes to immune pathways. The differentially expressed genes are indicated along with the direction of change in expression. ( C ) Quantitative-PCR of selected genes shows agreement with microarray results. The results obtained using the microarray platform were validated by examining the correlation between the expression levels in the microarray and qPCR results obtained for a subset of genes.

    Article Snippet: Following the washing procedures, the slides were immediately scanned using a Microarray Scanner (Model: Agilent G2565AA System) to minimize the environmental oxidation and loss of signal intensities.

    Techniques: Microarray, Mouse Assay, Functional Assay, Indirect Immunoperoxidase Assay, Expressing, Real-time Polymerase Chain Reaction