sb202190  (Selleck Chemicals)


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    Name:
    SB202190
    Description:
    SB202190 FHPI is a potent p38 MAPK inhibitor targeting p38α β with IC50 of 50 nM 100 nM in cell free assays sometimes used instead of SB 203580 to investigate potential roles for SAPK2a p38 in vivo SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase 1 SB202190 significantly suppresses Erastin dependent ferroptosis
    Catalog Number:
    s1077-25mg
    Price:
    147.0
    Size:
    25mg
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    Structured Review

    Selleck Chemicals sb202190
    SB202190
    SB202190 FHPI is a potent p38 MAPK inhibitor targeting p38α β with IC50 of 50 nM 100 nM in cell free assays sometimes used instead of SB 203580 to investigate potential roles for SAPK2a p38 in vivo SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase 1 SB202190 significantly suppresses Erastin dependent ferroptosis
    https://www.bioz.com/result/sb202190/product/Selleck Chemicals
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sb202190 - by Bioz Stars, 2021-03
    98/100 stars

    Images

    1) Product Images from "The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes"

    Article Title: The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    doi: 10.1016/j.jcmgh.2017.12.013

    Optimization of normal human and murine esophageal 3D organoid culture . Esophageal 3D organoid culture was optimized with the normal human esophageal epithelial cell line EPC2-hTERT in A–C and wild-type murine esophageal cells dissociated from epithelial sheets in D–F (ie, Noggin/R-Spondin1-conditioned medium, Wnt3A, A83-01, SB202190, gastrin, and nicotinamide) were added as indicated. KSFM contains 0.09 mM Ca 2+ (low Ca 2+ ), whereas KSFMC is supplemented with additional CaCl 2 to the final concentration of 0.6 mM Ca 2+ (high Ca 2+ ). The aDMEM medium contains 1 mM Ca 2+ . 3D culture products were photomicrographed under a phase-contrast microscopy and further recovered from Matrigel for H E staining in A and D . Organoids were defined as 3D structures with a diameter of 50 μm or larger. Note that 3D organoid formation was barely observed in aDMEM/F12 with or without unique supplements. Addition of unique supplements also limited organoid formation in KSFM. 3D organoids grown in KSFM showed a lobular pattern of basaloid cell expansion toward surrounding matrix and had a less explicit differentiation gradient as compared with 3D organoids formed in KSFMC. Average organoid size was determined in B and E . OFR was determined in C and F . Scale bar = 100 μm in phase-contrast images and 50 μm in H E staining. * P
    Figure Legend Snippet: Optimization of normal human and murine esophageal 3D organoid culture . Esophageal 3D organoid culture was optimized with the normal human esophageal epithelial cell line EPC2-hTERT in A–C and wild-type murine esophageal cells dissociated from epithelial sheets in D–F (ie, Noggin/R-Spondin1-conditioned medium, Wnt3A, A83-01, SB202190, gastrin, and nicotinamide) were added as indicated. KSFM contains 0.09 mM Ca 2+ (low Ca 2+ ), whereas KSFMC is supplemented with additional CaCl 2 to the final concentration of 0.6 mM Ca 2+ (high Ca 2+ ). The aDMEM medium contains 1 mM Ca 2+ . 3D culture products were photomicrographed under a phase-contrast microscopy and further recovered from Matrigel for H E staining in A and D . Organoids were defined as 3D structures with a diameter of 50 μm or larger. Note that 3D organoid formation was barely observed in aDMEM/F12 with or without unique supplements. Addition of unique supplements also limited organoid formation in KSFM. 3D organoids grown in KSFM showed a lobular pattern of basaloid cell expansion toward surrounding matrix and had a less explicit differentiation gradient as compared with 3D organoids formed in KSFMC. Average organoid size was determined in B and E . OFR was determined in C and F . Scale bar = 100 μm in phase-contrast images and 50 μm in H E staining. * P

    Techniques Used: Concentration Assay, Microscopy, Staining

    2) Product Images from "The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes"

    Article Title: The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    doi: 10.1016/j.jcmgh.2017.12.013

    Optimization of normal human and murine esophageal 3D organoid culture . Esophageal 3D organoid culture was optimized with the normal human esophageal epithelial cell line EPC2-hTERT in A–C and wild-type murine esophageal cells dissociated from epithelial sheets in D–F (ie, Noggin/R-Spondin1-conditioned medium, Wnt3A, A83-01, SB202190, gastrin, and nicotinamide) were added as indicated. KSFM contains 0.09 mM Ca 2+ (low Ca 2+ ), whereas KSFMC is supplemented with additional CaCl 2 to the final concentration of 0.6 mM Ca 2+ (high Ca 2+ ). The aDMEM medium contains 1 mM Ca 2+ . 3D culture products were photomicrographed under a phase-contrast microscopy and further recovered from Matrigel for H E staining in A and D . Organoids were defined as 3D structures with a diameter of 50 μm or larger. Note that 3D organoid formation was barely observed in aDMEM/F12 with or without unique supplements. Addition of unique supplements also limited organoid formation in KSFM. 3D organoids grown in KSFM showed a lobular pattern of basaloid cell expansion toward surrounding matrix and had a less explicit differentiation gradient as compared with 3D organoids formed in KSFMC. Average organoid size was determined in B and E . OFR was determined in C and F . Scale bar = 100 μm in phase-contrast images and 50 μm in H E staining. * P
    Figure Legend Snippet: Optimization of normal human and murine esophageal 3D organoid culture . Esophageal 3D organoid culture was optimized with the normal human esophageal epithelial cell line EPC2-hTERT in A–C and wild-type murine esophageal cells dissociated from epithelial sheets in D–F (ie, Noggin/R-Spondin1-conditioned medium, Wnt3A, A83-01, SB202190, gastrin, and nicotinamide) were added as indicated. KSFM contains 0.09 mM Ca 2+ (low Ca 2+ ), whereas KSFMC is supplemented with additional CaCl 2 to the final concentration of 0.6 mM Ca 2+ (high Ca 2+ ). The aDMEM medium contains 1 mM Ca 2+ . 3D culture products were photomicrographed under a phase-contrast microscopy and further recovered from Matrigel for H E staining in A and D . Organoids were defined as 3D structures with a diameter of 50 μm or larger. Note that 3D organoid formation was barely observed in aDMEM/F12 with or without unique supplements. Addition of unique supplements also limited organoid formation in KSFM. 3D organoids grown in KSFM showed a lobular pattern of basaloid cell expansion toward surrounding matrix and had a less explicit differentiation gradient as compared with 3D organoids formed in KSFMC. Average organoid size was determined in B and E . OFR was determined in C and F . Scale bar = 100 μm in phase-contrast images and 50 μm in H E staining. * P

    Techniques Used: Concentration Assay, Microscopy, Staining

    Related Articles

    other:

    Article Title: The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes
    Article Snippet: Among factors and agents originally used for murine esophageal 3D organoids, R-Spondin1 and Noggin, Wnt3A, or A83-01 improved OFR in passaged organoids in KSFMC; however, SB202190, a p38 mitogen-activated protein kinase inhibitor, impaired organoid formation by human cells.

    Article Title: The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes
    Article Snippet: Given the inhibitory effect of SB202190, human esophageal cells may be sensitive to p38 mitogen-activated protein kinase–mediated processes, raising the possibility that antioxidants may alleviate cellular stress and improve cell survival.

    Generated:

    Article Title: The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes
    Article Snippet: Telomerase-immortalized normal human esophageal epithelial cell line EPC2-hTERT and derivatives carrying GFP , DNMAML1 , or DNMAML1 Tet-Off , , were grown in keratinocyte-SFM (KSFM) medium containing 0.09 mM Ca2+ as described previously., Cell authentication was externally done by short-tandem repeat DNA profiling at the American Type Culture Collection (Manassas, VA). .. Murine esophageal 3D organoids were generated as described using advanced Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (aDMEM/F12) containing EGF, Noggin/R-Spondin1-conditioned medium, Wnt3A (R & D Systems, Minneapolis, MN), A83-01 (Sigma-Aldrich, St. Louis, MO), and SB202190 (Selleck Chemical, Huston, TX), unless the use of KSFM or modified KSFM containing 0.6 mM Ca2+ (KSFMC) was noted (see for media compositions). .. For ex vivo Notch1 deletion in 3D esophageal organoids generated from Notch1l oxP/loxP mice, organoids were incubated with Adenovirus expressing Cre recombinase or green fluorescent protein (GFP, control) (University of Iowa Gene Transfer Vector Core).

    Modification:

    Article Title: The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes
    Article Snippet: Telomerase-immortalized normal human esophageal epithelial cell line EPC2-hTERT and derivatives carrying GFP , DNMAML1 , or DNMAML1 Tet-Off , , were grown in keratinocyte-SFM (KSFM) medium containing 0.09 mM Ca2+ as described previously., Cell authentication was externally done by short-tandem repeat DNA profiling at the American Type Culture Collection (Manassas, VA). .. Murine esophageal 3D organoids were generated as described using advanced Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (aDMEM/F12) containing EGF, Noggin/R-Spondin1-conditioned medium, Wnt3A (R & D Systems, Minneapolis, MN), A83-01 (Sigma-Aldrich, St. Louis, MO), and SB202190 (Selleck Chemical, Huston, TX), unless the use of KSFM or modified KSFM containing 0.6 mM Ca2+ (KSFMC) was noted (see for media compositions). .. For ex vivo Notch1 deletion in 3D esophageal organoids generated from Notch1l oxP/loxP mice, organoids were incubated with Adenovirus expressing Cre recombinase or green fluorescent protein (GFP, control) (University of Iowa Gene Transfer Vector Core).

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    Selleck Chemicals sb202190
    Optimization of normal human and murine esophageal 3D organoid culture . Esophageal 3D organoid culture was optimized with the normal human esophageal epithelial cell line EPC2-hTERT in A–C and wild-type murine esophageal cells dissociated from epithelial sheets in D–F (ie, Noggin/R-Spondin1-conditioned medium, Wnt3A, A83-01, <t>SB202190,</t> gastrin, and nicotinamide) were added as indicated. KSFM contains 0.09 mM Ca 2+ (low Ca 2+ ), whereas KSFMC is supplemented with additional CaCl 2 to the final concentration of 0.6 mM Ca 2+ (high Ca 2+ ). The aDMEM medium contains 1 mM Ca 2+ . 3D culture products were photomicrographed under a phase-contrast microscopy and further recovered from Matrigel for H E staining in A and D . Organoids were defined as 3D structures with a diameter of 50 μm or larger. Note that 3D organoid formation was barely observed in aDMEM/F12 with or without unique supplements. Addition of unique supplements also limited organoid formation in KSFM. 3D organoids grown in KSFM showed a lobular pattern of basaloid cell expansion toward surrounding matrix and had a less explicit differentiation gradient as compared with 3D organoids formed in KSFMC. Average organoid size was determined in B and E . OFR was determined in C and F . Scale bar = 100 μm in phase-contrast images and 50 μm in H E staining. * P
    Sb202190, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sb202190/product/Selleck Chemicals
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sb202190 - by Bioz Stars, 2021-03
    98/100 stars
      Buy from Supplier

    95
    Selleck Chemicals sb202190 fhpi
    p38 inhibitor, <t>SB202190,</t> did not affect the antagonistic effect of CK2.3 on RANKL-induced osteoclastogenesis. ( A ) Concentration curve for SB202190 to determine the working concentration. ( B ) The effect of SB202190 on the antagonistic effect of CK2.3 on RANKL-induced osteoclastogenesis. a: denotes significant difference from Control without inhibitor, b: denotes significant difference from Control with inhibitor, c: denotes significant difference from RANKL at 10 ng/mL without inhibitor, d: denotes significant difference from RANKL at 10 ng/mL with inhibitor, and e: denotes significant difference from RANKL + CK2.3 at 100 nM without inhibitor.
    Sb202190 Fhpi, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sb202190 fhpi/product/Selleck Chemicals
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sb202190 fhpi - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    Image Search Results


    Optimization of normal human and murine esophageal 3D organoid culture . Esophageal 3D organoid culture was optimized with the normal human esophageal epithelial cell line EPC2-hTERT in A–C and wild-type murine esophageal cells dissociated from epithelial sheets in D–F (ie, Noggin/R-Spondin1-conditioned medium, Wnt3A, A83-01, SB202190, gastrin, and nicotinamide) were added as indicated. KSFM contains 0.09 mM Ca 2+ (low Ca 2+ ), whereas KSFMC is supplemented with additional CaCl 2 to the final concentration of 0.6 mM Ca 2+ (high Ca 2+ ). The aDMEM medium contains 1 mM Ca 2+ . 3D culture products were photomicrographed under a phase-contrast microscopy and further recovered from Matrigel for H E staining in A and D . Organoids were defined as 3D structures with a diameter of 50 μm or larger. Note that 3D organoid formation was barely observed in aDMEM/F12 with or without unique supplements. Addition of unique supplements also limited organoid formation in KSFM. 3D organoids grown in KSFM showed a lobular pattern of basaloid cell expansion toward surrounding matrix and had a less explicit differentiation gradient as compared with 3D organoids formed in KSFMC. Average organoid size was determined in B and E . OFR was determined in C and F . Scale bar = 100 μm in phase-contrast images and 50 μm in H E staining. * P

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial Changes

    doi: 10.1016/j.jcmgh.2017.12.013

    Figure Lengend Snippet: Optimization of normal human and murine esophageal 3D organoid culture . Esophageal 3D organoid culture was optimized with the normal human esophageal epithelial cell line EPC2-hTERT in A–C and wild-type murine esophageal cells dissociated from epithelial sheets in D–F (ie, Noggin/R-Spondin1-conditioned medium, Wnt3A, A83-01, SB202190, gastrin, and nicotinamide) were added as indicated. KSFM contains 0.09 mM Ca 2+ (low Ca 2+ ), whereas KSFMC is supplemented with additional CaCl 2 to the final concentration of 0.6 mM Ca 2+ (high Ca 2+ ). The aDMEM medium contains 1 mM Ca 2+ . 3D culture products were photomicrographed under a phase-contrast microscopy and further recovered from Matrigel for H E staining in A and D . Organoids were defined as 3D structures with a diameter of 50 μm or larger. Note that 3D organoid formation was barely observed in aDMEM/F12 with or without unique supplements. Addition of unique supplements also limited organoid formation in KSFM. 3D organoids grown in KSFM showed a lobular pattern of basaloid cell expansion toward surrounding matrix and had a less explicit differentiation gradient as compared with 3D organoids formed in KSFMC. Average organoid size was determined in B and E . OFR was determined in C and F . Scale bar = 100 μm in phase-contrast images and 50 μm in H E staining. * P

    Article Snippet: Given the inhibitory effect of SB202190, human esophageal cells may be sensitive to p38 mitogen-activated protein kinase–mediated processes, raising the possibility that antioxidants may alleviate cellular stress and improve cell survival.

    Techniques: Concentration Assay, Microscopy, Staining

    p38 inhibitor, SB202190, did not affect the antagonistic effect of CK2.3 on RANKL-induced osteoclastogenesis. ( A ) Concentration curve for SB202190 to determine the working concentration. ( B ) The effect of SB202190 on the antagonistic effect of CK2.3 on RANKL-induced osteoclastogenesis. a: denotes significant difference from Control without inhibitor, b: denotes significant difference from Control with inhibitor, c: denotes significant difference from RANKL at 10 ng/mL without inhibitor, d: denotes significant difference from RANKL at 10 ng/mL with inhibitor, and e: denotes significant difference from RANKL + CK2.3 at 100 nM without inhibitor.

    Journal: Journal of Developmental Biology

    Article Title: A Synthetic Peptide, CK2.3, Inhibits RANKL-Induced Osteoclastogenesis through BMPRIa and ERK Signaling Pathway

    doi: 10.3390/jdb8030012

    Figure Lengend Snippet: p38 inhibitor, SB202190, did not affect the antagonistic effect of CK2.3 on RANKL-induced osteoclastogenesis. ( A ) Concentration curve for SB202190 to determine the working concentration. ( B ) The effect of SB202190 on the antagonistic effect of CK2.3 on RANKL-induced osteoclastogenesis. a: denotes significant difference from Control without inhibitor, b: denotes significant difference from Control with inhibitor, c: denotes significant difference from RANKL at 10 ng/mL without inhibitor, d: denotes significant difference from RANKL at 10 ng/mL with inhibitor, and e: denotes significant difference from RANKL + CK2.3 at 100 nM without inhibitor.

    Article Snippet: The cells were treated as follows: RANKL, RANKL + inhibitor (U0126, CAPE, or SB202190), RANKL + CK2.3, RANKL + CK2.3 + inhibitor (U0126, CAPE, or SB202190), RANKL + BMP2, RANKL + BMP2 + inhibitor (U0126, CAPE, or SB202190), untreated, and untreated + inhibitor (U0126, CAPE, or SB202190).

    Techniques: Concentration Assay

    FSH protects GCs from H 2 O 2 -induced autophagic PCD through the PI3K-AKT pathway. Primary cultured GCs incubated with or without 200 μM H 2 O 2 for 1 h were then rinsed in PBS, and grown in serum-free medium containing 7.5 IU/ml FSH for 2 h. Perifosine (AKT inhibitor, 10 μM), SB203580 (MAPK14 inhibitor, 20 μM), U0126 (MAPK1/3 inhibitor, 3 μM) was added 30 min before FSH treatment. (A) qRT-PCR was performed to measure the mRNA levels of autophagy-related ( Atg ) genes in GCs. Expression data were normalized to that of Actb . ** Represents P

    Journal: Autophagy

    Article Title: Protective mechanism of FSH against oxidative damage in mouse ovarian granulosa cells by repressing autophagy

    doi: 10.1080/15548627.2017.1327941

    Figure Lengend Snippet: FSH protects GCs from H 2 O 2 -induced autophagic PCD through the PI3K-AKT pathway. Primary cultured GCs incubated with or without 200 μM H 2 O 2 for 1 h were then rinsed in PBS, and grown in serum-free medium containing 7.5 IU/ml FSH for 2 h. Perifosine (AKT inhibitor, 10 μM), SB203580 (MAPK14 inhibitor, 20 μM), U0126 (MAPK1/3 inhibitor, 3 μM) was added 30 min before FSH treatment. (A) qRT-PCR was performed to measure the mRNA levels of autophagy-related ( Atg ) genes in GCs. Expression data were normalized to that of Actb . ** Represents P

    Article Snippet: For FSH treatment, GCs exposed to 200 μM H2 O2 (Sigma-Aldrich, 216763–100ML) for 0 h (none) or 1 h were washed in PBS, and cultured with serum-free DMEM/F-12 containing 7.5 IU/ml FSH (Sigma-Aldrich, F4021) in the presence or absence of pepstatin A and E64 (10 μg/ml; Selleck, S7381 and S7379, respectively), perifosine (10 μM; Selleck, S1037), SB203580 (20 μM; Selleck, S1077), U0126 (3 μM; Selleck, S1102), torin 1 (100 nM; Selleck, S2827), sirtinol (100 μM; Selleck, S2804) or SRT1720 (100 μM; Selleck, S1129) for 0.5, 1, 2, or 3 h as indicated.

    Techniques: Cell Culture, Incubation, Quantitative RT-PCR, Expressing

    Involvement of p38 mitogen-activated protein kinase (MAPK) in MMP-9 induction in primary cultured astrocytes exposed to 2-chloroethanol. Notes: SB, SB202190; 2-CE, 2-chloroethanol. Cells in the exposure group and intervention groups were exposed to 30 mM 2-CE for 24 h. Cells in the intervention groups were pre-exposed with SB 1 h before 2-CE exposure. (A) Western blot analysis. Images were the representative results of four separate experiments for each group. (B) Densitometric analysis of western blots. The relative intensity of MMP-9 and p-p38 in arbitrary units was compared to β-actin. The ratios of phosphorylated p38 to native p38 was expressed as the p-p38/p38. (C) Quantitation of mRNA by real-time RT-PCR. The gene expression was normalized to GAPDH and presented as fold change vs. the control. Data were expressed as mean ± SD of four independent experiments performed on four batches of primary cultured astrocytes, and analyzed by One-way ANOVA. Significant difference was defined as p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Upregulation of Matrix Metalloproteinase-9 in Primary Cultured Rat Astrocytes Induced by 2-Chloroethanol Via MAPK Signal Pathways

    doi: 10.3389/fncel.2017.00218

    Figure Lengend Snippet: Involvement of p38 mitogen-activated protein kinase (MAPK) in MMP-9 induction in primary cultured astrocytes exposed to 2-chloroethanol. Notes: SB, SB202190; 2-CE, 2-chloroethanol. Cells in the exposure group and intervention groups were exposed to 30 mM 2-CE for 24 h. Cells in the intervention groups were pre-exposed with SB 1 h before 2-CE exposure. (A) Western blot analysis. Images were the representative results of four separate experiments for each group. (B) Densitometric analysis of western blots. The relative intensity of MMP-9 and p-p38 in arbitrary units was compared to β-actin. The ratios of phosphorylated p38 to native p38 was expressed as the p-p38/p38. (C) Quantitation of mRNA by real-time RT-PCR. The gene expression was normalized to GAPDH and presented as fold change vs. the control. Data were expressed as mean ± SD of four independent experiments performed on four batches of primary cultured astrocytes, and analyzed by One-way ANOVA. Significant difference was defined as p

    Article Snippet: SB202190, U0126, and SP600125 were purchased from Selleck (USA).

    Techniques: Cell Culture, Western Blot, Quantitation Assay, Quantitative RT-PCR, Expressing