Structured Review

ApexBio sb202190
Effect of PD98059 (PD), a p44/42 inhibitor, <t>SB202190</t> (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P
Sb202190, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sb202190/product/ApexBio
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sb202190 - by Bioz Stars, 2021-03
86/100 stars

Images

1) Product Images from "Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells"

Article Title: Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

doi: 10.1152/ajpgi.00275.2018

Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P
Figure Legend Snippet: Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P

Techniques Used: Isolation, Incubation, Western Blot

2) Product Images from "Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells"

Article Title: Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

doi: 10.1152/ajpgi.00275.2018

Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P
Figure Legend Snippet: Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P

Techniques Used: Isolation, Incubation, Western Blot

3) Product Images from "Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells"

Article Title: Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

doi: 10.1152/ajpgi.00275.2018

Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P
Figure Legend Snippet: Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P

Techniques Used: Isolation, Incubation, Western Blot

4) Product Images from "Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells"

Article Title: Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

doi: 10.1152/ajpgi.00275.2018

Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P
Figure Legend Snippet: Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P

Techniques Used: Isolation, Incubation, Western Blot

5) Product Images from "Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells"

Article Title: Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells

Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

doi: 10.1152/ajpgi.00275.2018

Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P
Figure Legend Snippet: Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P

Techniques Used: Isolation, Incubation, Western Blot

Related Articles

Inhibition:

Article Title: Pancreatic Physiology/Pathophysiology: P21-activated kinase 4 in pancreatic acinar cells is activated by numerous gastrointestinal hormones/neurotransmitters and growth factors by novel signaling, and its activation stimulates secretory/growth cascades
Article Snippet: The inactive control, PP3, did not have any effect on PAK4 phosphorylation or on the positive control ( , Lanes 9–12). confirmed the results obtained with Wortmannin ( , Lanes 7–9). .. To assess the possible effect of p44/42 or p38 inhibition on pS474 phosphorylation, we preincubated pancreatic acini for 1 h with either 10 µM PD98059 or 10 µM SB202190, respectively. .. When p44/42 activation was inhibited, the increment in the serine phosphorylation of PAK4 was significantly reduced by 86 ± 32% in the case of CCK-8 100 nM and 76 ± 39% with TPA ( , Lanes 7–8).

CCK-8 Assay:

Article Title: Pancreatic Physiology/Pathophysiology: P21-activated kinase 4 in pancreatic acinar cells is activated by numerous gastrointestinal hormones/neurotransmitters and growth factors by novel signaling, and its activation stimulates secretory/growth cascades
Article Snippet: However, preincubation with the p38 inhibitor, SB202190, decreased only the 0.3 nM CCK-8-stimulated phosphorylation ( , Lane 10). .. However, SB202190 did not have any effect on 100 nM CCK-8- or TPA-stimulated PAK4 phosphorylation ( , Lanes 11–12). .. PF-3758309 has been developed as a potent inhibitor of PAK4 and has been used in a number of studies to determine the role of PAK4 activation in various processes ( , ).

Article Title: Pancreatic Physiology/Pathophysiology: P21-activated kinase 4 in pancreatic acinar cells is activated by numerous gastrointestinal hormones/neurotransmitters and growth factors by novel signaling, and its activation stimulates secretory/growth cascades
Article Snippet: However, preincubation with the p38 inhibitor, SB202190, decreased only the 0.3 nM CCK-8-stimulated phosphorylation ( , Lane 10). .. However, SB202190 did not have any effect on 100 nM CCK-8- or TPA-stimulated PAK4 phosphorylation ( , Lanes 11–12). .. PF-3758309 has been developed as a potent inhibitor of PAK4 and has been used in a number of studies to determine the role of PAK4 activation in various processes ( , ).

other:

Article Title: Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells
Article Snippet: However, preincubation with the p44/42 and p38 inhibitors, PD98059 and SB202190, reduced the secretin-induced phosphorylation of CREB by 80 and 94%, respectively ( , lanes 3 and 4 ).

Article Title: Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells
Article Snippet: In the case of VIP, preincubation with PD98059 or SB202190, inhibited completely VIP-induced CREB phosphorylation by 96% ( , lanes 8 and 9 ).

Produced:

Article Title: Differential anti-tumour effects of MTH1 inhibitors in patient-derived 3D colorectal cancer cultures
Article Snippet: Organoids were seeded in droplets of 8 μl each, consisting of 60% Matrigel (Corning) mixed with organoid medium. .. The composition of organoid medium is: advanced DMEM/F12 (Gibco) supplemented with 1% penicillin-streptocycin (Gibco), 10 mM HEPES buffer (Lonza), 2 mM GlutaMax (Gibco), 2% B27 supplement (Gibco), 1 mM NAC, 100 ng/ml noggin (produced in house), 0.5 μM A8301 (SignalChem), and 10 μM SB202190 (APExBIO). ..

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    ApexBio sb202190
    Effect of PD98059 (PD), a p44/42 inhibitor, <t>SB202190</t> (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P
    Sb202190, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sb202190/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sb202190 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Hormones, Neurotransmitters, Growth Factors, Receptors, and Signaling: Cyclic AMP-dependent protein kinase A and EPAC mediate VIP and secretin stimulation of PAK4 and activation of Na+,K+-ATPase in pancreatic acinar cells

    doi: 10.1152/ajpgi.00275.2018

    Figure Lengend Snippet: Effect of PD98059 (PD), a p44/42 inhibitor, SB202190 (SB), a p38 inhibitor, and SP600125 (SP), a JNK inhibitor, on PAK4 phosphorylation ( A ) and on CREB phosphorylation ( B ) by secretin and VIP in rat pancreatic acini. Isolated pancreatic acini were incubated in the absence or presence of PD98059 (10 µM), SB202190 (10 µM) or SP600125 (20 µM) for 1 h and then incubated with no addition (control), secretin (10 nM) of VIP (10 nM) for 15 min and then lysed. Western blots were analyzed using anti-pS474 PAK4 or pS133 CREB and, as loading control, antitotal PAK4 or antitotal CREB. Bands were visualized using chemiluminescence and quantified by densitometry. Top : results of a representative blot of 3 independent experiments are shown. Bottom : means ± SE of at least 4 independent experiments. Results are expressed as percentages of stimulation over the control group. * P

    Article Snippet: However, preincubation with the p44/42 and p38 inhibitors, PD98059 and SB202190, reduced the secretin-induced phosphorylation of CREB by 80 and 94%, respectively ( , lanes 3 and 4 ).

    Techniques: Isolation, Incubation, Western Blot