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GenScript sb100x
Sb100x, supplied by GenScript, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sb100x/product/GenScript
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
sb100x - by Bioz Stars, 2020-09
88/100 stars

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Related Articles

Clone Assay:

Article Title: DNA transposition by protein transduction of the piggyBac transposase from lentiviral Gag precursors
Article Snippet: .. To generate vectors based on the SB system, a codon-optimized (CO) version of SB100X was synthesized by GenScript and cloned into the SacII/SacII sites of pCMV-SB100X ( ) to generate pCMV-SB100XCO. .. Variants of SB100XCO with an N- or a C-terminal extension of five amino acid residues (pCMV-5aa-SB100XCO and pCMV-SB100XCO-5aa, respectively) were constructed by inserting SacII-digested PCR products (generated with primer sets YJ018F-YJ029R and YJ020F-YJ030R) into SacII-digested pCMV-SB100XCO.

Synthesized:

Article Title: DNA transposition by protein transduction of the piggyBac transposase from lentiviral Gag precursors
Article Snippet: .. To generate vectors based on the SB system, a codon-optimized (CO) version of SB100X was synthesized by GenScript and cloned into the SacII/SacII sites of pCMV-SB100X ( ) to generate pCMV-SB100XCO. .. Variants of SB100XCO with an N- or a C-terminal extension of five amino acid residues (pCMV-5aa-SB100XCO and pCMV-SB100XCO-5aa, respectively) were constructed by inserting SacII-digested PCR products (generated with primer sets YJ018F-YJ029R and YJ020F-YJ030R) into SacII-digested pCMV-SB100XCO.

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  • 93
    GenScript hyperactive sb100x version
    Flexibility index (FI) of the original SB and <t>SB100X</t> transposases. (A) Cartoon representation of SB100X transposase with hyperactive mutations shown in orange color using spheres. The RNase H-like structural motif is shown by green (beta-sheet) and purple (alpha-helices) colors. Catalytic residues are shown in red color using spheres. (B) The difference of FI values between the original SB and SB100X transposases is mapped onto the cartoon structure of SB transposase. (C) Backbone flexibility index is shown versus the residue number for SB (blue) and SB100X (red) transposases. Positive values indicate flexibility, while negative values indicate rigidity. Dashed line corresponds to zero (isostatic case) and is shown as guide to the eye. Secondary structure elements are indicated above the graph and colored as in the structure shown in (A).
    Hyperactive Sb100x Version, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hyperactive sb100x version/product/GenScript
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hyperactive sb100x version - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    88
    GenScript sb100x
    Flexibility index (FI) of the original SB and <t>SB100X</t> transposases. (A) Cartoon representation of SB100X transposase with hyperactive mutations shown in orange color using spheres. The RNase H-like structural motif is shown by green (beta-sheet) and purple (alpha-helices) colors. Catalytic residues are shown in red color using spheres. (B) The difference of FI values between the original SB and SB100X transposases is mapped onto the cartoon structure of SB transposase. (C) Backbone flexibility index is shown versus the residue number for SB (blue) and SB100X (red) transposases. Positive values indicate flexibility, while negative values indicate rigidity. Dashed line corresponds to zero (isostatic case) and is shown as guide to the eye. Secondary structure elements are indicated above the graph and colored as in the structure shown in (A).
    Sb100x, supplied by GenScript, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sb100x/product/GenScript
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sb100x - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Flexibility index (FI) of the original SB and SB100X transposases. (A) Cartoon representation of SB100X transposase with hyperactive mutations shown in orange color using spheres. The RNase H-like structural motif is shown by green (beta-sheet) and purple (alpha-helices) colors. Catalytic residues are shown in red color using spheres. (B) The difference of FI values between the original SB and SB100X transposases is mapped onto the cartoon structure of SB transposase. (C) Backbone flexibility index is shown versus the residue number for SB (blue) and SB100X (red) transposases. Positive values indicate flexibility, while negative values indicate rigidity. Dashed line corresponds to zero (isostatic case) and is shown as guide to the eye. Secondary structure elements are indicated above the graph and colored as in the structure shown in (A).

    Journal: Proteins

    Article Title: Rigidity and flexibility characteristics of DD[E/D]-transposases Mos1 and Sleeping Beauty.

    doi: 10.1002/prot.25653

    Figure Lengend Snippet: Flexibility index (FI) of the original SB and SB100X transposases. (A) Cartoon representation of SB100X transposase with hyperactive mutations shown in orange color using spheres. The RNase H-like structural motif is shown by green (beta-sheet) and purple (alpha-helices) colors. Catalytic residues are shown in red color using spheres. (B) The difference of FI values between the original SB and SB100X transposases is mapped onto the cartoon structure of SB transposase. (C) Backbone flexibility index is shown versus the residue number for SB (blue) and SB100X (red) transposases. Positive values indicate flexibility, while negative values indicate rigidity. Dashed line corresponds to zero (isostatic case) and is shown as guide to the eye. Secondary structure elements are indicated above the graph and colored as in the structure shown in (A).

    Article Snippet: DNA plasmids encoding the catalytic domain containing amino acid residues 111–340 of the original SB transposase sequence or its hyperactive SB100X version were ordered from GenScript USA (Piscataway, NJ).

    Techniques:

    Determination of T m by extrinsic and intrinsic fluorescence. (A) ANS fluorescence spectra are shown at temperatures 15, 55, and 80 o C for original SB (solid circles) and SB100X (open circles) transposases. (B) Temperature-induced change of ANS fluorescence measured at λ max for original SB (solid circles) and SB100X (open circles) transposases. Solid lines represent non-linear Boltzmann sigmoidal fits of experimental data. Determined T m values are 65 ± 1.0 and 66 ± 1.5 o C for SB and SB100X transposases, respectively. (C) Intrinsic fluorescence spectra collected for original SB (solid symbols) and SB100X (open symbols) transposases at 25 o C and urea concentrations of 0 M (circles) and 5 M (diamonds). Red spectral shift in the presence of urea indicates protein unfolding. (D) T m values determined for original SB (solid circles) and SB100X (open circles) transposases at different urea concentrations. T m values determined by extrapolation to 0 M urea concentration are 62 ± 1.0 and 63 ± 1.0 o C for SB and SB100X transposases, respectively.

    Journal: Proteins

    Article Title: Rigidity and flexibility characteristics of DD[E/D]-transposases Mos1 and Sleeping Beauty.

    doi: 10.1002/prot.25653

    Figure Lengend Snippet: Determination of T m by extrinsic and intrinsic fluorescence. (A) ANS fluorescence spectra are shown at temperatures 15, 55, and 80 o C for original SB (solid circles) and SB100X (open circles) transposases. (B) Temperature-induced change of ANS fluorescence measured at λ max for original SB (solid circles) and SB100X (open circles) transposases. Solid lines represent non-linear Boltzmann sigmoidal fits of experimental data. Determined T m values are 65 ± 1.0 and 66 ± 1.5 o C for SB and SB100X transposases, respectively. (C) Intrinsic fluorescence spectra collected for original SB (solid symbols) and SB100X (open symbols) transposases at 25 o C and urea concentrations of 0 M (circles) and 5 M (diamonds). Red spectral shift in the presence of urea indicates protein unfolding. (D) T m values determined for original SB (solid circles) and SB100X (open circles) transposases at different urea concentrations. T m values determined by extrapolation to 0 M urea concentration are 62 ± 1.0 and 63 ± 1.0 o C for SB and SB100X transposases, respectively.

    Article Snippet: DNA plasmids encoding the catalytic domain containing amino acid residues 111–340 of the original SB transposase sequence or its hyperactive SB100X version were ordered from GenScript USA (Piscataway, NJ).

    Techniques: Fluorescence, Concentration Assay