Journal: Vaccines
Article Title: Humoral and Cellular Response Induced by Primary Series and Booster Doses of mRNA Coronavirus Disease 2019 Vaccine in Patients with Cardiovascular Disease: A Longitudinal Study
doi: 10.3390/vaccines12070786
Figure Lengend Snippet: Cellular response against Omicron BA.1 before and after the booster dose. Cellular response after the booster dose by INF-γ secreting T cells. Assessed by ELISpot after stimulation by SARS-CoV-2 (Omicron BA.1, S1 scan) Human IFN-γ kits and expressed as SFU/10 4 PBMCs. The paired Wilcoxon signed-rank test was used to compare the Omicron BA.1-specific T cell responses before and after the booster dose. ( A , B ) B1 and B2 indicate the first and second vaccine booster dose, respectively. INF-γ, interferon-γ; SFC, spot-forming units; PBMCs, peripheral blood mononuclear cells; ELISpot, Enzyme-Linked immunoSPOT.
Article Snippet: Cryopreserved PBMCs from whole blood samples were thawed, and 4 × 10 4 PBMCs were seeded per well and stimulated with the SARS-CoV-2 S1 domain of the spike protein scanning peptide pool (Mabtech AB, Stockholm, Sweden) at a concentration of 2 μg/mL of each peptide for 14 h at 37 °C and 5% CO 2 .
Techniques: Enzyme-linked Immunospot