Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Development of a high-sensitivity and short-duration fluorescence in situ hybridization method for viral mRNA detection in HEK 293T cells

doi: 10.3389/fcimb.2022.960938

Figure Lengend Snippet: Specificities and sources of primary and secondary antibodies.

Article Snippet: SARS-CoV-2 1/2 Spike protein(2B3E5) , , Mouse IgG, monoclonal , Cell Signaling Technology (Danvers, MA, United States).

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Development of a high-sensitivity and short-duration fluorescence in situ hybridization method for viral mRNA detection in HEK 293T cells

doi: 10.3389/fcimb.2022.960938

Figure Lengend Snippet: Detection of SARS-CoV-2 E mRNA and E/S protein in HEK 293T cells at 24 h after transfection with SARS-CoV-2 E and S plasmids. (A) SARS-CoV-2 E mRNA (with mixed probes containing BME001 and BME002), SARS-CoV-2 E, and SARS-CoV-2 S are shown in red, green, and blue, respectively. (B) Total mRNAs (detected by Oligo dT probes), SARS-CoV-2 E, and SARS-CoV-2 S are shown in red, green, and blue, respectively. (C) Negative controls by using secondary antibodies. Objective lens: 60×; Scale bar: 10 µm; Nu, nucleus.

Article Snippet: SARS-CoV-2 1/2 Spike protein(2B3E5) , , Mouse IgG, monoclonal , Cell Signaling Technology (Danvers, MA, United States).

Techniques: Transfection

Journal: Cell Reports

Article Title: Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infection

doi: 10.1016/j.celrep.2022.110714

Figure Lengend Snippet:

Article Snippet: The following anti-SARS-CoV-2 antibodies were used for immunohistochemistry: rabbit polyclonal anti-SARS-CoV Nucleoprotein (Novus Biological, Littleton, CO, USA), mouse monoclonal anti-SARS-CoV-2 Spike clone 2B3E5 (This antibody was used in this study as clone E7U60, which was the pre-production clone ID of clone 2B3E5; Cell Signaling Technology).

Techniques: Recombinant, Luciferase, Electron Microscopy, Isolation, Software, In Vivo, Modification, Magnetic Beads