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anti alpha sarcoglycan  (Novocastra)

 
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    Novocastra anti alpha sarcoglycan
    Anti Alpha Sarcoglycan, supplied by Novocastra, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti alpha sarcoglycan/product/Novocastra
    Average 86 stars, based on 1 article reviews
    anti alpha sarcoglycan - by Bioz Stars, 2025-03
    86/100 stars

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    ( A ) Dystrophin, nNOS, and <t>alpha-sarcoglycan</t> immunohistochemistry of tibialis anterior (TA) in wild-type (WT), d45–48, d45–47, d45–49, and mdx mice, at the age of 1 and 3 months. ( B ) Western blot analysis for dystrophin in WT, d45–48, d45–47, d45–49, and mdx mice, at the ages of 1 and 3 months. ( C ) Western blot analysis for dystrophin-homolog utrophin in WT, d45–48, d45–47, d45–49, and mdx mice, at the ages of 1 and 3 months. ( D ) Western blot analysis for nNOS in WT, d45–48, d45–47, d45–49, and mdx mice, at the ages of 1 and 3 months. ( E ) Relative Dp427 protein levels (normalized by total protein band intensity) in WT, d45–48, d45–47, d45–49, and mdx mice ( n = 3). ( F ) Relative Up395 protein levels (normalized by total protein band intensity) in WT, d45–48, d45–47, d45–49, and mdx mice ( n = 3). ( G ) Relative nNOS protein levels (normalized by total protein band intensity) in WT, d45–48, d45–47, d45–49, and mdx mice ( n = 3). Bar: mean ± SD; *p < 0.05, **p < 0.01. Scale bar means 100 µm.
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    Image Search Results


    ( A ) Dystrophin, nNOS, and alpha-sarcoglycan immunohistochemistry of tibialis anterior (TA) in wild-type (WT), d45–48, d45–47, d45–49, and mdx mice, at the age of 1 and 3 months. ( B ) Western blot analysis for dystrophin in WT, d45–48, d45–47, d45–49, and mdx mice, at the ages of 1 and 3 months. ( C ) Western blot analysis for dystrophin-homolog utrophin in WT, d45–48, d45–47, d45–49, and mdx mice, at the ages of 1 and 3 months. ( D ) Western blot analysis for nNOS in WT, d45–48, d45–47, d45–49, and mdx mice, at the ages of 1 and 3 months. ( E ) Relative Dp427 protein levels (normalized by total protein band intensity) in WT, d45–48, d45–47, d45–49, and mdx mice ( n = 3). ( F ) Relative Up395 protein levels (normalized by total protein band intensity) in WT, d45–48, d45–47, d45–49, and mdx mice ( n = 3). ( G ) Relative nNOS protein levels (normalized by total protein band intensity) in WT, d45–48, d45–47, d45–49, and mdx mice ( n = 3). Bar: mean ± SD; *p < 0.05, **p < 0.01. Scale bar means 100 µm.

    Journal: eLife

    Article Title: Becker muscular dystrophy mice showed site-specific decay of type IIa fibers with capillary change in skeletal muscle

    doi: 10.7554/eLife.100665

    Figure Lengend Snippet: ( A ) Dystrophin, nNOS, and alpha-sarcoglycan immunohistochemistry of tibialis anterior (TA) in wild-type (WT), d45–48, d45–47, d45–49, and mdx mice, at the age of 1 and 3 months. ( B ) Western blot analysis for dystrophin in WT, d45–48, d45–47, d45–49, and mdx mice, at the ages of 1 and 3 months. ( C ) Western blot analysis for dystrophin-homolog utrophin in WT, d45–48, d45–47, d45–49, and mdx mice, at the ages of 1 and 3 months. ( D ) Western blot analysis for nNOS in WT, d45–48, d45–47, d45–49, and mdx mice, at the ages of 1 and 3 months. ( E ) Relative Dp427 protein levels (normalized by total protein band intensity) in WT, d45–48, d45–47, d45–49, and mdx mice ( n = 3). ( F ) Relative Up395 protein levels (normalized by total protein band intensity) in WT, d45–48, d45–47, d45–49, and mdx mice ( n = 3). ( G ) Relative nNOS protein levels (normalized by total protein band intensity) in WT, d45–48, d45–47, d45–49, and mdx mice ( n = 3). Bar: mean ± SD; *p < 0.05, **p < 0.01. Scale bar means 100 µm.

    Article Snippet: The primary antibodies were as follows: mouse anti-dystrophin (NCL-DYS2, Leica Biosystems), rabbit anti-nNOS (61-7000, Invitrogen), mouse anti-alpha-sarcoglycan (NCL-a-SARC, Leica Biosystems), mouse anti-MYH-1 (6H1, DSHB), mouse anti-MYH-2 (SC-71, DSHB), mouse anti-MYH-4 (BF-F3, DSHB), mouse anti-MYH-7 (BA-D5, Millipore), and rat anti-PECAM-1 (550274, BD Bioscience).

    Techniques: Immunohistochemistry, Western Blot

    Journal: Cell reports

    Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner

    doi: 10.1016/j.celrep.2024.114149

    Figure Lengend Snippet:

    Article Snippet: Mouse monoclonal anti-alpha Sarcoglycan , Developmental Studies Hybridoma Bank (DSHB) , Cat# IVD3(1)A9; RRID:AB_2185657.

    Techniques: Virus, Recombinant, Electron Microscopy, Plasmid Preparation, SYBR Green Assay, Membrane, Blocking Assay, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Protein Extraction, Clone Assay, Expressing, Software, Microscopy, Real-time Polymerase Chain Reaction

    Dilution factor for individual antibodies

    Journal: Cureus

    Article Title: Clinicopathological Profile of Muscle Diseases Presenting the Adult Population in Northern India: Preliminary Analysis in a Limited Resource Setting

    doi: 10.7759/cureus.60084

    Figure Lengend Snippet: Dilution factor for individual antibodies

    Article Snippet: Alpha Sarcoglycan , Leica Novocastra liquid mouse monoclonal (adhalin) , Ad1/20A6 , IgG1 , 1:100.

    Techniques:

    Becker’s muscular dystrophy case: (A) H&E stain showing myophagocytosis (x40), (B) H&E stain showing regenerating fibers (x40), (C) dystrophin N IHC stain showing mild positivity (x20), (D) alpha sarcoglycan IHC stain showing positivity (x20), (E) gamma sarcoglycan IHC stain showing positivity (x20), (F) Spectrin IHC stain showing positivity (x20).

    Journal: Cureus

    Article Title: Clinicopathological Profile of Muscle Diseases Presenting the Adult Population in Northern India: Preliminary Analysis in a Limited Resource Setting

    doi: 10.7759/cureus.60084

    Figure Lengend Snippet: Becker’s muscular dystrophy case: (A) H&E stain showing myophagocytosis (x40), (B) H&E stain showing regenerating fibers (x40), (C) dystrophin N IHC stain showing mild positivity (x20), (D) alpha sarcoglycan IHC stain showing positivity (x20), (E) gamma sarcoglycan IHC stain showing positivity (x20), (F) Spectrin IHC stain showing positivity (x20).

    Article Snippet: Alpha Sarcoglycan , Leica Novocastra liquid mouse monoclonal (adhalin) , Ad1/20A6 , IgG1 , 1:100.

    Techniques: Staining

    Dysferlinopathy case: (A) NADH staining showing Type I and Type II fibers (x20), (B) alpha sarcoglycan IHC stain showing positivity (x20), (C) dysferlin IHC stain showing negativity (x20), (D) dystrophin N IHC stain showing positivity (x20).

    Journal: Cureus

    Article Title: Clinicopathological Profile of Muscle Diseases Presenting the Adult Population in Northern India: Preliminary Analysis in a Limited Resource Setting

    doi: 10.7759/cureus.60084

    Figure Lengend Snippet: Dysferlinopathy case: (A) NADH staining showing Type I and Type II fibers (x20), (B) alpha sarcoglycan IHC stain showing positivity (x20), (C) dysferlin IHC stain showing negativity (x20), (D) dystrophin N IHC stain showing positivity (x20).

    Article Snippet: Alpha Sarcoglycan , Leica Novocastra liquid mouse monoclonal (adhalin) , Ad1/20A6 , IgG1 , 1:100.

    Techniques: Staining

    Alpha sarcoglycanopathy case: (A) H&E stain showing variation in muscle fiber (x10), (B) Alpha sarcoglycan IHC stain showing negativity (x20), (C) beta sarcoglycan IHC stain showing positivity (x20), (D) Gamma sarcoglycan IHC stain showing positivity (x20).

    Journal: Cureus

    Article Title: Clinicopathological Profile of Muscle Diseases Presenting the Adult Population in Northern India: Preliminary Analysis in a Limited Resource Setting

    doi: 10.7759/cureus.60084

    Figure Lengend Snippet: Alpha sarcoglycanopathy case: (A) H&E stain showing variation in muscle fiber (x10), (B) Alpha sarcoglycan IHC stain showing negativity (x20), (C) beta sarcoglycan IHC stain showing positivity (x20), (D) Gamma sarcoglycan IHC stain showing positivity (x20).

    Article Snippet: Alpha Sarcoglycan , Leica Novocastra liquid mouse monoclonal (adhalin) , Ad1/20A6 , IgG1 , 1:100.

    Techniques: Staining