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Regulation of genes involved in the AMC by QS in B . glumae . SAH, S-adenosyl-L-homocysteine; <t>SAM,</t> S -adenosyl- L -methionine; AhcY, SAH hydrolase (gene ID: bglu_1g01990); MetK, SAM <t>synthase</t> (gene ID: bglu_1g33680); MetF, methylenetetrahydrofolate reductase (gene ID: bglu_1g02010); MetE1, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 1 (gene ID: bglu_1g08430); MetE2, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 2 (gene ID: bglu_2g04930); MetH1, methionine synthase 1 (gene ID: bglu_1g32280); MetH2, methionine synthase 2 (gene ID: bglu_1g32290); octanoyl-ACP, octanoyl acyl-carrier protein; C8-HSL, N -octanoyl homoserine lactone; MetX, homoserine O-acetyltransferase (gene ID: bglu_1g33830); MetZ, O-acetylhomoserine sulfhydrylase (gene ID: bglu_2g08500); MetC, cystathionine beta-lyase (gene ID: bglu_1g15950). T shape indicates inhibition; dashed T indicates putative inhibition. Bold arrows indicate activation, and the asterisk denotes QsmR-dependent expression of metE2 in the absence of MetH1/H2.
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Regulation of genes involved in the AMC by QS in B . glumae . SAH, S-adenosyl-L-homocysteine; <t>SAM,</t> S -adenosyl- L -methionine; AhcY, SAH hydrolase (gene ID: bglu_1g01990); MetK, SAM <t>synthase</t> (gene ID: bglu_1g33680); MetF, methylenetetrahydrofolate reductase (gene ID: bglu_1g02010); MetE1, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 1 (gene ID: bglu_1g08430); MetE2, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 2 (gene ID: bglu_2g04930); MetH1, methionine synthase 1 (gene ID: bglu_1g32280); MetH2, methionine synthase 2 (gene ID: bglu_1g32290); octanoyl-ACP, octanoyl acyl-carrier protein; C8-HSL, N -octanoyl homoserine lactone; MetX, homoserine O-acetyltransferase (gene ID: bglu_1g33830); MetZ, O-acetylhomoserine sulfhydrylase (gene ID: bglu_2g08500); MetC, cystathionine beta-lyase (gene ID: bglu_1g15950). T shape indicates inhibition; dashed T indicates putative inhibition. Bold arrows indicate activation, and the asterisk denotes QsmR-dependent expression of metE2 in the absence of MetH1/H2.
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Regulation of genes involved in the AMC by QS in B . glumae . SAH, S-adenosyl-L-homocysteine; <t>SAM,</t> S -adenosyl- L -methionine; AhcY, SAH hydrolase (gene ID: bglu_1g01990); MetK, SAM <t>synthase</t> (gene ID: bglu_1g33680); MetF, methylenetetrahydrofolate reductase (gene ID: bglu_1g02010); MetE1, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 1 (gene ID: bglu_1g08430); MetE2, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 2 (gene ID: bglu_2g04930); MetH1, methionine synthase 1 (gene ID: bglu_1g32280); MetH2, methionine synthase 2 (gene ID: bglu_1g32290); octanoyl-ACP, octanoyl acyl-carrier protein; C8-HSL, N -octanoyl homoserine lactone; MetX, homoserine O-acetyltransferase (gene ID: bglu_1g33830); MetZ, O-acetylhomoserine sulfhydrylase (gene ID: bglu_2g08500); MetC, cystathionine beta-lyase (gene ID: bglu_1g15950). T shape indicates inhibition; dashed T indicates putative inhibition. Bold arrows indicate activation, and the asterisk denotes QsmR-dependent expression of metE2 in the absence of MetH1/H2.
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Regulation of genes involved in the AMC by QS in B . glumae . SAH, S-adenosyl-L-homocysteine; SAM, S -adenosyl- L -methionine; AhcY, SAH hydrolase (gene ID: bglu_1g01990); MetK, SAM synthase (gene ID: bglu_1g33680); MetF, methylenetetrahydrofolate reductase (gene ID: bglu_1g02010); MetE1, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 1 (gene ID: bglu_1g08430); MetE2, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 2 (gene ID: bglu_2g04930); MetH1, methionine synthase 1 (gene ID: bglu_1g32280); MetH2, methionine synthase 2 (gene ID: bglu_1g32290); octanoyl-ACP, octanoyl acyl-carrier protein; C8-HSL, N -octanoyl homoserine lactone; MetX, homoserine O-acetyltransferase (gene ID: bglu_1g33830); MetZ, O-acetylhomoserine sulfhydrylase (gene ID: bglu_2g08500); MetC, cystathionine beta-lyase (gene ID: bglu_1g15950). T shape indicates inhibition; dashed T indicates putative inhibition. Bold arrows indicate activation, and the asterisk denotes QsmR-dependent expression of metE2 in the absence of MetH1/H2.

Journal: Scientific Reports

Article Title: Unraveling the role of quorum sensing-dependent metabolic homeostasis of the activated methyl cycle in a cooperative population of Burkholderia glumae

doi: 10.1038/s41598-019-47460-6

Figure Lengend Snippet: Regulation of genes involved in the AMC by QS in B . glumae . SAH, S-adenosyl-L-homocysteine; SAM, S -adenosyl- L -methionine; AhcY, SAH hydrolase (gene ID: bglu_1g01990); MetK, SAM synthase (gene ID: bglu_1g33680); MetF, methylenetetrahydrofolate reductase (gene ID: bglu_1g02010); MetE1, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 1 (gene ID: bglu_1g08430); MetE2, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 2 (gene ID: bglu_2g04930); MetH1, methionine synthase 1 (gene ID: bglu_1g32280); MetH2, methionine synthase 2 (gene ID: bglu_1g32290); octanoyl-ACP, octanoyl acyl-carrier protein; C8-HSL, N -octanoyl homoserine lactone; MetX, homoserine O-acetyltransferase (gene ID: bglu_1g33830); MetZ, O-acetylhomoserine sulfhydrylase (gene ID: bglu_2g08500); MetC, cystathionine beta-lyase (gene ID: bglu_1g15950). T shape indicates inhibition; dashed T indicates putative inhibition. Bold arrows indicate activation, and the asterisk denotes QsmR-dependent expression of metE2 in the absence of MetH1/H2.

Article Snippet: The blocked membrane was incubated with a rabbit polyclonal antibody against the N-terminal region of SAM synthase (MetK; NBP1-55120; Novus Biologicals, LLC, Centennial, CO, USA).

Techniques: Inhibition, Activation Assay, Expressing