sabouraud dextrose agar  (thermo fisher)


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    thermo fisher sabouraud dextrose agar
    Validamycin A increases trehalose levels in Aspergillus flavus conidia with delayed conidial germination and decreased fungal adherence. (a) Aspergillus flavus ATCC 204304 was cultured at 37°C on <t>Sabouraud</t> dextrose agar for five days with or without 1 μ g/mL validamycin A. Trehalose assays were performed to measure trehalose levels in the conidia using glucose oxidase assays. Data are presented as means ± SE from three biological replicates. ∗∗∗ P value
    Sabouraud Dextrose Agar, supplied by thermo fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    sabouraud dextrose agar - by Bioz Stars, 2022-05
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    Images

    1) Product Images from "The Inhibitory Effect of Validamycin A on Aspergillus flavus"

    Article Title: The Inhibitory Effect of Validamycin A on Aspergillus flavus

    Journal: International Journal of Microbiology

    doi: 10.1155/2020/3972415

    Validamycin A increases trehalose levels in Aspergillus flavus conidia with delayed conidial germination and decreased fungal adherence. (a) Aspergillus flavus ATCC 204304 was cultured at 37°C on Sabouraud dextrose agar for five days with or without 1 μ g/mL validamycin A. Trehalose assays were performed to measure trehalose levels in the conidia using glucose oxidase assays. Data are presented as means ± SE from three biological replicates. ∗∗∗ P value
    Figure Legend Snippet: Validamycin A increases trehalose levels in Aspergillus flavus conidia with delayed conidial germination and decreased fungal adherence. (a) Aspergillus flavus ATCC 204304 was cultured at 37°C on Sabouraud dextrose agar for five days with or without 1 μ g/mL validamycin A. Trehalose assays were performed to measure trehalose levels in the conidia using glucose oxidase assays. Data are presented as means ± SE from three biological replicates. ∗∗∗ P value

    Techniques Used: Cell Culture

    2) Product Images from "Thermogenic Characterization and Antifungal Susceptibility of Candida auris by Microcalorimetry"

    Article Title: Thermogenic Characterization and Antifungal Susceptibility of Candida auris by Microcalorimetry

    Journal: Journal of Fungi

    doi: 10.3390/jof5040103

    Pictures of Candida spp., colonies growth onto Sabouraud dextrose agar. C. auris exhibited smaller colony size compared to most Candida spp. after 24 h incubation time at 37 °C.
    Figure Legend Snippet: Pictures of Candida spp., colonies growth onto Sabouraud dextrose agar. C. auris exhibited smaller colony size compared to most Candida spp. after 24 h incubation time at 37 °C.

    Techniques Used: Incubation

    Biofilm of C. auris CBS14916 ( a ) and C. albicans ATCC 90028 ( b ) formed on porous glass beads. CFU number of C. auris and C. albicans dislodged from glass beads by sonication after incubation in either Sabouraud or RPMI 1640 broth at 37 °C for 24, 48, and 72 h.
    Figure Legend Snippet: Biofilm of C. auris CBS14916 ( a ) and C. albicans ATCC 90028 ( b ) formed on porous glass beads. CFU number of C. auris and C. albicans dislodged from glass beads by sonication after incubation in either Sabouraud or RPMI 1640 broth at 37 °C for 24, 48, and 72 h.

    Techniques Used: Sonication, Incubation

    3) Product Images from "Novel Taxa Associated with Human Fungal Black-Grain Mycetomas: Emarellia grisea gen. nov., sp. nov., and Emarellia paragrisea sp. nov."

    Article Title: Novel Taxa Associated with Human Fungal Black-Grain Mycetomas: Emarellia grisea gen. nov., sp. nov., and Emarellia paragrisea sp. nov.

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00477-16

    Colonial and microscopic appearance of Emarellia grisea (NCPF 7066) (A and C) and E. paragrisea (NCPF 7611) (B and D). The colonial appearance after 2 weeks of culture on Sabouraud dextrose agar at 30°C (A and B) and the microscopic appearance
    Figure Legend Snippet: Colonial and microscopic appearance of Emarellia grisea (NCPF 7066) (A and C) and E. paragrisea (NCPF 7611) (B and D). The colonial appearance after 2 weeks of culture on Sabouraud dextrose agar at 30°C (A and B) and the microscopic appearance

    Techniques Used:

    4) Product Images from "A Novel Topical Combination Ointment with Antimicrobial Activity against Methicillin-Resistant Streptococcus aureus, Gram-Negative Superbugs, Yeasts, and Dermatophytic Fungi"

    Article Title: A Novel Topical Combination Ointment with Antimicrobial Activity against Methicillin-Resistant Streptococcus aureus, Gram-Negative Superbugs, Yeasts, and Dermatophytic Fungi

    Journal: Current Therapeutic Research, Clinical and Experimental

    doi: 10.1016/j.curtheres.2016.07.001

    Inhibition of Candida albicans on Sabouraud agar by Bensal HP (Sonar Products Inc., Carlstadt, NJ).
    Figure Legend Snippet: Inhibition of Candida albicans on Sabouraud agar by Bensal HP (Sonar Products Inc., Carlstadt, NJ).

    Techniques Used: Inhibition

    Inhibition of Trichophyton mentagrophytes on Sabouraud agar by Bensal HP (Sonar Products Inc., Carlstadt, NJ).
    Figure Legend Snippet: Inhibition of Trichophyton mentagrophytes on Sabouraud agar by Bensal HP (Sonar Products Inc., Carlstadt, NJ).

    Techniques Used: Inhibition

    5) Product Images from "Rapid Identification of Clinically Relevant Members of the Genus Exophiala by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Description of Two Novel Species, Exophiala campbellii and Exophiala lavatrina"

    Article Title: Rapid Identification of Clinically Relevant Members of the Genus Exophiala by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Description of Two Novel Species, Exophiala campbellii and Exophiala lavatrina

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.02459-16

    Colonial and microscopic morphological appearance of Exophiala lavatrina sp. nov. (a to d) Colony appearance of NCPF 7893 (a), NCPF 7898 (b), NCPF 7899 (c), and NCPF 7900 (d) on Sabouraud dextrose agar after 14 days at 30°C. (e to i) Conidiophores, conidiogenous cells, and conidia of NCPF 7893. Bars = 10 μm.
    Figure Legend Snippet: Colonial and microscopic morphological appearance of Exophiala lavatrina sp. nov. (a to d) Colony appearance of NCPF 7893 (a), NCPF 7898 (b), NCPF 7899 (c), and NCPF 7900 (d) on Sabouraud dextrose agar after 14 days at 30°C. (e to i) Conidiophores, conidiogenous cells, and conidia of NCPF 7893. Bars = 10 μm.

    Techniques Used:

    Colonial and microscopic morphological appearance of Exophiala campbellii sp. nov. (NCPF 2274) (a) Colony on Sabouraud dextrose agar after 14 days at 30°C. (b to h) Conidiophores, conidiogenous cells, and conidia. (c) Phialophora -like phialidic state with collarettes and conidia. Bars = 10 μm.
    Figure Legend Snippet: Colonial and microscopic morphological appearance of Exophiala campbellii sp. nov. (NCPF 2274) (a) Colony on Sabouraud dextrose agar after 14 days at 30°C. (b to h) Conidiophores, conidiogenous cells, and conidia. (c) Phialophora -like phialidic state with collarettes and conidia. Bars = 10 μm.

    Techniques Used:

    6) Product Images from "Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil"

    Article Title: Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01783

    Hemolytic activity of the environmental and clinical isolates of C. tropicalis. C. albicans SC5314, ATCC90028 and C. tropicalis ATCC13803 reference strains. Yeast cells were grown on the surface of Sabouraud Dextrose Agar added fresh sheep blood and 3% glucose for 48 h at 37°C in an atmosphere of 5% CO 2 . The hemolytic index (HI) was determined by the ratio between the diameter of the colony and the diameter of the colony plus hemolysis zone.
    Figure Legend Snippet: Hemolytic activity of the environmental and clinical isolates of C. tropicalis. C. albicans SC5314, ATCC90028 and C. tropicalis ATCC13803 reference strains. Yeast cells were grown on the surface of Sabouraud Dextrose Agar added fresh sheep blood and 3% glucose for 48 h at 37°C in an atmosphere of 5% CO 2 . The hemolytic index (HI) was determined by the ratio between the diameter of the colony and the diameter of the colony plus hemolysis zone.

    Techniques Used: Activity Assay

    7) Product Images from "First isolation of Ascotricha chartarum from bronchoalveolar lavage of two patients with pulmonary infections"

    Article Title: First isolation of Ascotricha chartarum from bronchoalveolar lavage of two patients with pulmonary infections

    Journal: New Microbes and New Infections

    doi: 10.1016/j.nmni.2018.12.002

    (a) Potassium hydroxide–calcofluor mount showing branched septate hyphae of Ascotricha chartarum in bronchoalveolae of patient 1. (b) Culture of bronchoalveolar lavage of patient 1 yielding several yellow-coloured colonies of A. chartarum (Kw277/17) on Sabouraud dextrose agar after 5 days of incubation at 30°C.
    Figure Legend Snippet: (a) Potassium hydroxide–calcofluor mount showing branched septate hyphae of Ascotricha chartarum in bronchoalveolae of patient 1. (b) Culture of bronchoalveolar lavage of patient 1 yielding several yellow-coloured colonies of A. chartarum (Kw277/17) on Sabouraud dextrose agar after 5 days of incubation at 30°C.

    Techniques Used: Incubation

    8) Product Images from "A fatal invasive Scedosporium apiospermum pulmonary infection in an adult patient with malignant lung adenocarcinoma"

    Article Title: A fatal invasive Scedosporium apiospermum pulmonary infection in an adult patient with malignant lung adenocarcinoma

    Journal: Current Medical Mycology

    doi: 10.18502/cmm.6.3.3982

    Colony of Scedosporium apiospermum complex on Sabouraud dextrose agar medium (The colony appearance is cottony and velvety, with the color being changed from white to grey.)
    Figure Legend Snippet: Colony of Scedosporium apiospermum complex on Sabouraud dextrose agar medium (The colony appearance is cottony and velvety, with the color being changed from white to grey.)

    Techniques Used:

    9) Product Images from "In vitro antifungal activity of Cinnamomum zeylanicum bark and leaf essential oils against Candida albicans and Candida auris"

    Article Title: In vitro antifungal activity of Cinnamomum zeylanicum bark and leaf essential oils against Candida albicans and Candida auris

    Journal: Applied Microbiology and Biotechnology

    doi: 10.1007/s00253-020-10829-z

    Photographs depicting the hemolysis of horse blood agar (supplemented with 3% w/v of glucose) induced by different concentrations of bark CEO (1, 2) and leaf CEO (3, 4). Panel a shows pure bark CEO (a) and pure leaf CEO (k) served as positive control; Sabouraud dextrose broth +0.5% Tween 20 served as negative control (j); bark CEO 4% (b); bark CEO 2% (c); bark CEO 1% (d); bark CEO 0.5% (e); bark CEO 0.25% (f); bark CEO 0.125% (g); bark CEO 0.06% (h); bark 0.03% (i); leaf CEO 4% (l); leaf CEO 2% (m); leaf CEO 1% (n); leaf CEO 0.5% (o); leaf CEO 0.25% (p); leaf CEO 0.125% (q); leaf CEO 0.06% (r); and leaf CEO 0.03% (s)
    Figure Legend Snippet: Photographs depicting the hemolysis of horse blood agar (supplemented with 3% w/v of glucose) induced by different concentrations of bark CEO (1, 2) and leaf CEO (3, 4). Panel a shows pure bark CEO (a) and pure leaf CEO (k) served as positive control; Sabouraud dextrose broth +0.5% Tween 20 served as negative control (j); bark CEO 4% (b); bark CEO 2% (c); bark CEO 1% (d); bark CEO 0.5% (e); bark CEO 0.25% (f); bark CEO 0.125% (g); bark CEO 0.06% (h); bark 0.03% (i); leaf CEO 4% (l); leaf CEO 2% (m); leaf CEO 1% (n); leaf CEO 0.5% (o); leaf CEO 0.25% (p); leaf CEO 0.125% (q); leaf CEO 0.06% (r); and leaf CEO 0.03% (s)

    Techniques Used: Positive Control, Negative Control

    10) Product Images from "Profiling of bacterial and fungal microbial communities in cystic fibrosis sputum using RNA"

    Article Title: Profiling of bacterial and fungal microbial communities in cystic fibrosis sputum using RNA

    Journal: bioRxiv

    doi: 10.1101/336230

    Culture analysis and NanoString analysis of sputum series from six hospitalized patients. Culture of E1-E6 sputum series on sheep blood agar, PIA, and Sabouraud dextrose agar plates. Plates were incubated for at least 48 h before imaging. Samples were collected on Day 0 (date of admission for treatment of exacerbation) and the days post treatment is shown for subsequent samples. For each series, RNA was analyzed by NanoString and data above background are shown for the bacterial and fungus targeting probes included in the code set. Exacerbation series E1 showed high levels of Exophiala dermatitidis in plate cultures only after extended incubation for all days and the plate from Day 0 is shown as an example. The clinical culture data for the day 0 samples are shown in Table 2 . The NanoString data in the graphs for each sample are the same data as shown in Fig. 3 , and are presented here for ease of comparison.
    Figure Legend Snippet: Culture analysis and NanoString analysis of sputum series from six hospitalized patients. Culture of E1-E6 sputum series on sheep blood agar, PIA, and Sabouraud dextrose agar plates. Plates were incubated for at least 48 h before imaging. Samples were collected on Day 0 (date of admission for treatment of exacerbation) and the days post treatment is shown for subsequent samples. For each series, RNA was analyzed by NanoString and data above background are shown for the bacterial and fungus targeting probes included in the code set. Exacerbation series E1 showed high levels of Exophiala dermatitidis in plate cultures only after extended incubation for all days and the plate from Day 0 is shown as an example. The clinical culture data for the day 0 samples are shown in Table 2 . The NanoString data in the graphs for each sample are the same data as shown in Fig. 3 , and are presented here for ease of comparison.

    Techniques Used: Incubation, Imaging

    11) Product Images from "Paradoxical Growth of Candida albicans in the Presence of Caspofungin Is Associated with Multiple Cell Wall Rearrangements and Decreased Virulence"

    Article Title: Paradoxical Growth of Candida albicans in the Presence of Caspofungin Is Associated with Multiple Cell Wall Rearrangements and Decreased Virulence

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00946-13

    Virulence of C. albicans cells pregrown with high caspofungin concentrations. Cells exhibiting paradoxical growth were obtained from C. albicans strain CL8102 after growth in Sabouraud liquid supplemented with 8 mg/liter CAS at 30°C and shaking
    Figure Legend Snippet: Virulence of C. albicans cells pregrown with high caspofungin concentrations. Cells exhibiting paradoxical growth were obtained from C. albicans strain CL8102 after growth in Sabouraud liquid supplemented with 8 mg/liter CAS at 30°C and shaking

    Techniques Used:

    12) Product Images from "Gene Expression Response of Trichophyton rubrum during Coculture on Keratinocytes Exposed to Antifungal Agents"

    Article Title: Gene Expression Response of Trichophyton rubrum during Coculture on Keratinocytes Exposed to Antifungal Agents

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2015/180535

    Coculture of Trichophyton rubrum in HaCaT cells exposed to antifungal agents. The T. rubrum solution (1 × 10 7 conidia/mL) was preincubated in Sabouraud medium for 7 h, 2.5 × 10 5 keratinocytes/mL were added, and the culture was exposed to the antifungal agents for 24 h. (a) Control; (b) 0.0162 μ M terbinafine; (c) 4.32 μ M α -solanine; (d) 0.288 μ M trans -chalcone.
    Figure Legend Snippet: Coculture of Trichophyton rubrum in HaCaT cells exposed to antifungal agents. The T. rubrum solution (1 × 10 7 conidia/mL) was preincubated in Sabouraud medium for 7 h, 2.5 × 10 5 keratinocytes/mL were added, and the culture was exposed to the antifungal agents for 24 h. (a) Control; (b) 0.0162 μ M terbinafine; (c) 4.32 μ M α -solanine; (d) 0.288 μ M trans -chalcone.

    Techniques Used:

    13) Product Images from "Novel Taxa Associated with Human Fungal Black-Grain Mycetomas: Emarellia grisea gen. nov., sp. nov., and Emarellia paragrisea sp. nov."

    Article Title: Novel Taxa Associated with Human Fungal Black-Grain Mycetomas: Emarellia grisea gen. nov., sp. nov., and Emarellia paragrisea sp. nov.

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00477-16

    Colonial and microscopic appearance of Emarellia grisea (NCPF 7066) (A and C) and E. paragrisea (NCPF 7611) (B and D). The colonial appearance after 2 weeks of culture on Sabouraud dextrose agar at 30°C (A and B) and the microscopic appearance
    Figure Legend Snippet: Colonial and microscopic appearance of Emarellia grisea (NCPF 7066) (A and C) and E. paragrisea (NCPF 7611) (B and D). The colonial appearance after 2 weeks of culture on Sabouraud dextrose agar at 30°C (A and B) and the microscopic appearance

    Techniques Used:

    14) Product Images from "Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates"

    Article Title: Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates

    Journal: Journal of Fungi

    doi: 10.3390/jof7010033

    Drug susceptibility. ( A ) Biofilm formation-phenotype dependent susceptibility testing where inoculum was prepared from cells after 1 day of growth on Sabouraud dextrose agar (SDA) (gray boxes) and after 8 days of growth (white boxes) on the same plates, when colonies had fully developed morphotypes. For each substance tested, the values for 1 and 8-day inoculum are depicted for each group (LBF, IBF, and HBF). Red lines: EUCAST clinical breakpoint (R > ); green lines, susceptible cut-off (S ≤). *: statistical significance ( B , C ) Effect of antifungal drugs on cell viability in pre-formed biofilms of selected biofilm-forming isolates tested in ( B ) RPMI (Roswell Park Memorial Institute )or ( C ) YEPD(yeast extract peptone dextrose) media.
    Figure Legend Snippet: Drug susceptibility. ( A ) Biofilm formation-phenotype dependent susceptibility testing where inoculum was prepared from cells after 1 day of growth on Sabouraud dextrose agar (SDA) (gray boxes) and after 8 days of growth (white boxes) on the same plates, when colonies had fully developed morphotypes. For each substance tested, the values for 1 and 8-day inoculum are depicted for each group (LBF, IBF, and HBF). Red lines: EUCAST clinical breakpoint (R > ); green lines, susceptible cut-off (S ≤). *: statistical significance ( B , C ) Effect of antifungal drugs on cell viability in pre-formed biofilms of selected biofilm-forming isolates tested in ( B ) RPMI (Roswell Park Memorial Institute )or ( C ) YEPD(yeast extract peptone dextrose) media.

    Techniques Used:

    15) Product Images from "Tinea corporis on the stump leg with Trichophyton rubrum infection"

    Article Title: Tinea corporis on the stump leg with Trichophyton rubrum infection

    Journal: Medical Mycology Case Reports

    doi: 10.1016/j.mmcr.2015.07.001

    (a) 10% KOH preparation of the scales revealing endothrix spores in the vellus hair (black arrow, 400×). (b) Stunted, white downy colonies developed at the culture on Sabouraud dextrose agar (SDA, 25 °C for 3 weeks), with a pale yellow-brown reverse pigment. (c) Slide culture showed atypical slender clavate macroconidia of T. rubrum (white arrow, 400×).
    Figure Legend Snippet: (a) 10% KOH preparation of the scales revealing endothrix spores in the vellus hair (black arrow, 400×). (b) Stunted, white downy colonies developed at the culture on Sabouraud dextrose agar (SDA, 25 °C for 3 weeks), with a pale yellow-brown reverse pigment. (c) Slide culture showed atypical slender clavate macroconidia of T. rubrum (white arrow, 400×).

    Techniques Used:

    16) Product Images from "Novel Taxa Associated with Human Fungal Black-Grain Mycetomas: Emarellia grisea gen. nov., sp. nov., and Emarellia paragrisea sp. nov."

    Article Title: Novel Taxa Associated with Human Fungal Black-Grain Mycetomas: Emarellia grisea gen. nov., sp. nov., and Emarellia paragrisea sp. nov.

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00477-16

    Colonial and microscopic appearance of Emarellia grisea (NCPF 7066) (A and C) and E. paragrisea (NCPF 7611) (B and D). The colonial appearance after 2 weeks of culture on Sabouraud dextrose agar at 30°C (A and B) and the microscopic appearance
    Figure Legend Snippet: Colonial and microscopic appearance of Emarellia grisea (NCPF 7066) (A and C) and E. paragrisea (NCPF 7611) (B and D). The colonial appearance after 2 weeks of culture on Sabouraud dextrose agar at 30°C (A and B) and the microscopic appearance

    Techniques Used:

    17) Product Images from "Two cases of fungal keratitis caused by Metarhizium anisopliae"

    Article Title: Two cases of fungal keratitis caused by Metarhizium anisopliae

    Journal: Medical Mycology Case Reports

    doi: 10.1016/j.mmcr.2018.03.003

    Morphologically different colony types of the B10964 (A, B), Georgia isolate, and B11022 (C, D), Missouri isolate, Metarhizium anisopliae isolates after 7 days of growth on Sabouraud dextrose agar at 25 °C (BBL, Becton, Dickinson and Company, Franklin Lakes, NJ). Colonies are floccose with a dense heaped center that was either light with a dark green ring (B10964) or yellow-green (B11022), both with a white fringe. The reverse of both isolates was a brownish orange color.
    Figure Legend Snippet: Morphologically different colony types of the B10964 (A, B), Georgia isolate, and B11022 (C, D), Missouri isolate, Metarhizium anisopliae isolates after 7 days of growth on Sabouraud dextrose agar at 25 °C (BBL, Becton, Dickinson and Company, Franklin Lakes, NJ). Colonies are floccose with a dense heaped center that was either light with a dark green ring (B10964) or yellow-green (B11022), both with a white fringe. The reverse of both isolates was a brownish orange color.

    Techniques Used:

    18) Product Images from "Epidemiology of Epizootic Lymphangitis Among Carthorses in Ethiopia"

    Article Title: Epidemiology of Epizootic Lymphangitis Among Carthorses in Ethiopia

    Journal: Frontiers in Veterinary Science

    doi: 10.3389/fvets.2021.762937

    Cultural appearances of Histoplasma capsulatum variety farciminosum . (A) 52 days old, primary culture of mycelial colonies on Brain Heart Infusion Aagar (with 5% horse blood and 0.005% chloramphenicol); (B) 48 days old sub-culture of the mycelial form on Sabouraud Dextrose Agar (with 2.5% glycerol and 0.005% chloramphenicol).
    Figure Legend Snippet: Cultural appearances of Histoplasma capsulatum variety farciminosum . (A) 52 days old, primary culture of mycelial colonies on Brain Heart Infusion Aagar (with 5% horse blood and 0.005% chloramphenicol); (B) 48 days old sub-culture of the mycelial form on Sabouraud Dextrose Agar (with 2.5% glycerol and 0.005% chloramphenicol).

    Techniques Used:

    19) Product Images from "Changing in the Epidemiology of Tinea Capitis among School Children in Egypt"

    Article Title: Changing in the Epidemiology of Tinea Capitis among School Children in Egypt

    Journal: Annals of Dermatology

    doi: 10.5021/ad.2017.29.1.13

    Isolated dermatophytes. (A) Microsporum canis on Sabouraud dextrose agar (SDA). (B) M. audouinii on SDA. (C) Microscopic picture showing macroconidia of M. canis . (D) Microscopic picture showing pectinate bodies of M. audouinii .
    Figure Legend Snippet: Isolated dermatophytes. (A) Microsporum canis on Sabouraud dextrose agar (SDA). (B) M. audouinii on SDA. (C) Microscopic picture showing macroconidia of M. canis . (D) Microscopic picture showing pectinate bodies of M. audouinii .

    Techniques Used: Isolation

    20) Product Images from "Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis"

    Article Title: Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis

    Journal: mBio

    doi: 10.1128/mBio.01937-21

    Effect of antibiotics on C. albicans virulence. (A) Fungal burdens in cortisone-immunosuppressed Candida -infected mice receiving sucrose or a combination of sucrose and penicillin in the drinking water. Mice were sacrificed at day 5, and tongue homogenates were plated on Sabouraud dextrose agar supplemented with chloramphenicol (10 μg/ml) for C. albicans quantification. In mice receiving sucrose, penicillin treatment resulted in significantly higher ( P = 0.0026) C. albicans burdens than with no antibiotic treatment. In contrast, in mice not receiving sucrose, penicillin treatment significantly reduced fungal burdens ( P = 0.0208) compared to that with no antibiotic treatment, suggesting that this treatment may reduce bacteria that have a synergistic effect with C. albicans and further underlying the differences in the bacterial compositions between sucrose-treated and untreated groups. The two antibiotic-treated groups (with and without sucrose) had similar fungal burdens. Results are from 1 to 2 mouse experiments with 5 to 12 mice/group. (B) Representative mucosal biofilm lesions on the tongues of antibiotic-treated groups with and without sucrose treatment. Both antibiotic-treated groups had similar biofilm lesions.
    Figure Legend Snippet: Effect of antibiotics on C. albicans virulence. (A) Fungal burdens in cortisone-immunosuppressed Candida -infected mice receiving sucrose or a combination of sucrose and penicillin in the drinking water. Mice were sacrificed at day 5, and tongue homogenates were plated on Sabouraud dextrose agar supplemented with chloramphenicol (10 μg/ml) for C. albicans quantification. In mice receiving sucrose, penicillin treatment resulted in significantly higher ( P = 0.0026) C. albicans burdens than with no antibiotic treatment. In contrast, in mice not receiving sucrose, penicillin treatment significantly reduced fungal burdens ( P = 0.0208) compared to that with no antibiotic treatment, suggesting that this treatment may reduce bacteria that have a synergistic effect with C. albicans and further underlying the differences in the bacterial compositions between sucrose-treated and untreated groups. The two antibiotic-treated groups (with and without sucrose) had similar fungal burdens. Results are from 1 to 2 mouse experiments with 5 to 12 mice/group. (B) Representative mucosal biofilm lesions on the tongues of antibiotic-treated groups with and without sucrose treatment. Both antibiotic-treated groups had similar biofilm lesions.

    Techniques Used: Infection, Mouse Assay

    Effect of Lactobacillus johnsonii isolated from sucrose-treated mice on C. albicans planktonic and sessile growth in vitro. (A) A murine L. johnsonii isolate was grown alone or in combination with C. albicans in 5 ml MRS broth aerobically in 5% CO 2 for up to 24 h. Aliquots (100 μl) were collected every 90 min, plated in triplicates, and incubated anaerobically on MRS agar (for lactobacilli) or aerobically on Sabouraud dextrose agar supplemented with 10 μg ml −1 of chloramphenicol (for Candida ) for CFU determinations. In some experiments, fresh media were added after aliquot collection at each time point (dotted lines). There was impaired planktonic growth of C. albicans (but not L. johnsonii ) after 6 h of coculture with L. johnsonii , regardless of medium addition. This growth inhibition became even more striking after 24 h. (B) A significant dose-dependent inhibition was noted for fungal planktonic growth after 24 h with fungal/bacterial (Ca:Lj) cell ratios ranging from 1:1 to 1:1,000. *, P
    Figure Legend Snippet: Effect of Lactobacillus johnsonii isolated from sucrose-treated mice on C. albicans planktonic and sessile growth in vitro. (A) A murine L. johnsonii isolate was grown alone or in combination with C. albicans in 5 ml MRS broth aerobically in 5% CO 2 for up to 24 h. Aliquots (100 μl) were collected every 90 min, plated in triplicates, and incubated anaerobically on MRS agar (for lactobacilli) or aerobically on Sabouraud dextrose agar supplemented with 10 μg ml −1 of chloramphenicol (for Candida ) for CFU determinations. In some experiments, fresh media were added after aliquot collection at each time point (dotted lines). There was impaired planktonic growth of C. albicans (but not L. johnsonii ) after 6 h of coculture with L. johnsonii , regardless of medium addition. This growth inhibition became even more striking after 24 h. (B) A significant dose-dependent inhibition was noted for fungal planktonic growth after 24 h with fungal/bacterial (Ca:Lj) cell ratios ranging from 1:1 to 1:1,000. *, P

    Techniques Used: Isolation, Mouse Assay, In Vitro, Incubation, Inhibition

    21) Product Images from "Novel Taxa Associated with Human Fungal Black-Grain Mycetomas: Emarellia grisea gen. nov., sp. nov., and Emarellia paragrisea sp. nov."

    Article Title: Novel Taxa Associated with Human Fungal Black-Grain Mycetomas: Emarellia grisea gen. nov., sp. nov., and Emarellia paragrisea sp. nov.

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00477-16

    Colonial and microscopic appearance of Emarellia grisea (NCPF 7066) (A and C) and E. paragrisea (NCPF 7611) (B and D). The colonial appearance after 2 weeks of culture on Sabouraud dextrose agar at 30°C (A and B) and the microscopic appearance
    Figure Legend Snippet: Colonial and microscopic appearance of Emarellia grisea (NCPF 7066) (A and C) and E. paragrisea (NCPF 7611) (B and D). The colonial appearance after 2 weeks of culture on Sabouraud dextrose agar at 30°C (A and B) and the microscopic appearance

    Techniques Used:

    22) Product Images from "The Inhibitory Effect of Validamycin A on Aspergillus flavus"

    Article Title: The Inhibitory Effect of Validamycin A on Aspergillus flavus

    Journal: International Journal of Microbiology

    doi: 10.1155/2020/3972415

    Validamycin A increases trehalose levels in Aspergillus flavus conidia with delayed conidial germination and decreased fungal adherence. (a) Aspergillus flavus ATCC 204304 was cultured at 37°C on Sabouraud dextrose agar for five days with or without 1 μ g/mL validamycin A. Trehalose assays were performed to measure trehalose levels in the conidia using glucose oxidase assays. Data are presented as means ± SE from three biological replicates. ∗∗∗ P value
    Figure Legend Snippet: Validamycin A increases trehalose levels in Aspergillus flavus conidia with delayed conidial germination and decreased fungal adherence. (a) Aspergillus flavus ATCC 204304 was cultured at 37°C on Sabouraud dextrose agar for five days with or without 1 μ g/mL validamycin A. Trehalose assays were performed to measure trehalose levels in the conidia using glucose oxidase assays. Data are presented as means ± SE from three biological replicates. ∗∗∗ P value

    Techniques Used: Cell Culture

    23) Product Images from "Thermogenic Characterization and Antifungal Susceptibility of Candida auris by Microcalorimetry"

    Article Title: Thermogenic Characterization and Antifungal Susceptibility of Candida auris by Microcalorimetry

    Journal: Journal of Fungi

    doi: 10.3390/jof5040103

    Pictures of Candida spp., colonies growth onto Sabouraud dextrose agar. C. auris exhibited smaller colony size compared to most Candida spp. after 24 h incubation time at 37 °C.
    Figure Legend Snippet: Pictures of Candida spp., colonies growth onto Sabouraud dextrose agar. C. auris exhibited smaller colony size compared to most Candida spp. after 24 h incubation time at 37 °C.

    Techniques Used: Incubation

    Biofilm of C. auris CBS14916 ( a ) and C. albicans ATCC 90028 ( b ) formed on porous glass beads. CFU number of C. auris and C. albicans dislodged from glass beads by sonication after incubation in either Sabouraud or RPMI 1640 broth at 37 °C for 24, 48, and 72 h.
    Figure Legend Snippet: Biofilm of C. auris CBS14916 ( a ) and C. albicans ATCC 90028 ( b ) formed on porous glass beads. CFU number of C. auris and C. albicans dislodged from glass beads by sonication after incubation in either Sabouraud or RPMI 1640 broth at 37 °C for 24, 48, and 72 h.

    Techniques Used: Sonication, Incubation

    24) Product Images from "Rapid Identification of Clinically Relevant Members of the Genus Exophiala by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Description of Two Novel Species, Exophiala campbellii and Exophiala lavatrina"

    Article Title: Rapid Identification of Clinically Relevant Members of the Genus Exophiala by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Description of Two Novel Species, Exophiala campbellii and Exophiala lavatrina

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.02459-16

    Colonial and microscopic morphological appearance of Exophiala lavatrina sp. nov. (a to d) Colony appearance of NCPF 7893 (a), NCPF 7898 (b), NCPF 7899 (c), and NCPF 7900 (d) on Sabouraud dextrose agar after 14 days at 30°C. (e to i) Conidiophores, conidiogenous cells, and conidia of NCPF 7893. Bars = 10 μm.
    Figure Legend Snippet: Colonial and microscopic morphological appearance of Exophiala lavatrina sp. nov. (a to d) Colony appearance of NCPF 7893 (a), NCPF 7898 (b), NCPF 7899 (c), and NCPF 7900 (d) on Sabouraud dextrose agar after 14 days at 30°C. (e to i) Conidiophores, conidiogenous cells, and conidia of NCPF 7893. Bars = 10 μm.

    Techniques Used:

    Colonial and microscopic morphological appearance of Exophiala campbellii sp. nov. (NCPF 2274) (a) Colony on Sabouraud dextrose agar after 14 days at 30°C. (b to h) Conidiophores, conidiogenous cells, and conidia. (c) Phialophora -like phialidic state with collarettes and conidia. Bars = 10 μm.
    Figure Legend Snippet: Colonial and microscopic morphological appearance of Exophiala campbellii sp. nov. (NCPF 2274) (a) Colony on Sabouraud dextrose agar after 14 days at 30°C. (b to h) Conidiophores, conidiogenous cells, and conidia. (c) Phialophora -like phialidic state with collarettes and conidia. Bars = 10 μm.

    Techniques Used:

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    • sabouraud agar plates  (Thermo Fisher)
    95
    Thermo Fisher sabouraud agar plates
    Cultivation of microorganisms from murine feces. Representative pictures of the heterogeneously overgrown Columbia and MHF blood agar (microaerobic, 37 °C), MacConkey No. 3 agar (aerobic, 37 °C), as well as the non-overgrown <t>Sabouraud</t> glucose agar (aerobic, room temperature) used to isolate individual bacterial species. From these and similar plates, colonies of different phenotypes were cultured in pure culture, isolated, and then identified by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry.
    Sabouraud Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sabouraud agar plates/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sabouraud agar plates - by Bioz Stars, 2022-05
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    sabouraud dextrose agar  (thermo fisher)
    86
    thermo fisher sabouraud dextrose agar
    Validamycin A increases trehalose levels in Aspergillus flavus conidia with delayed conidial germination and decreased fungal adherence. (a) Aspergillus flavus ATCC 204304 was cultured at 37°C on <t>Sabouraud</t> dextrose agar for five days with or without 1 μ g/mL validamycin A. Trehalose assays were performed to measure trehalose levels in the conidia using glucose oxidase assays. Data are presented as means ± SE from three biological replicates. ∗∗∗ P value
    Sabouraud Dextrose Agar, supplied by thermo fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sabouraud dextrose agar/product/thermo fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sabouraud dextrose agar - by Bioz Stars, 2022-05
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    sabouraud dextrose agar plate  (Thermo Fisher)
    96
    Thermo Fisher sabouraud dextrose agar plate
    (Top) Isolate on <t>Sabouraud</t> dextrose agar. (Bottom) Wet prep (×100 magnification) of isolate showing budding yeast cells, arthroconidia, hyphae, and pseudohyphae.
    Sabouraud Dextrose Agar Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sabouraud dextrose agar plate/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sabouraud dextrose agar plate - by Bioz Stars, 2022-05
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    Cultivation of microorganisms from murine feces. Representative pictures of the heterogeneously overgrown Columbia and MHF blood agar (microaerobic, 37 °C), MacConkey No. 3 agar (aerobic, 37 °C), as well as the non-overgrown Sabouraud glucose agar (aerobic, room temperature) used to isolate individual bacterial species. From these and similar plates, colonies of different phenotypes were cultured in pure culture, isolated, and then identified by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry.

    Journal: International Journal of Molecular Sciences

    Article Title: Depletion of Lipocalin 2 (LCN2) in Mice Leads to Dysbiosis and Persistent Colonization with Segmented Filamentous Bacteria

    doi: 10.3390/ijms222313156

    Figure Lengend Snippet: Cultivation of microorganisms from murine feces. Representative pictures of the heterogeneously overgrown Columbia and MHF blood agar (microaerobic, 37 °C), MacConkey No. 3 agar (aerobic, 37 °C), as well as the non-overgrown Sabouraud glucose agar (aerobic, room temperature) used to isolate individual bacterial species. From these and similar plates, colonies of different phenotypes were cultured in pure culture, isolated, and then identified by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry.

    Article Snippet: Sabouraud agar plates were incubated for one week aerobically at room temperature.

    Techniques: Cell Culture, Isolation, Mass Spectrometry

    Effect of mitochondrial inhibitors during capsule induction. Cells from H99 strain were grown in Sabouraud liquid medium at 30°C (basal) and then transferred to 10% Sabouraud buffered at pH 7.3 with 50 mM MOPS in the presence of the highest concentration of antimycin A (3.6 μM) (A,B) , SHAM (4 mM) (C,D) , and rotenone (250 μM) (E,F) for 18 h. In each case, control samples incubated only with the solvent (EtOH or DMSO) or without any solvent (control) were carried out in parallel. After the incubation time, capsule size (A,C,E) was measured by suspending the cells in India ink as described in Section “Materials and Methods.” Asterisks indicate significant differences ( p

    Journal: Frontiers in Microbiology

    Article Title: Capsule Enlargement in Cryptococcus neoformans Is Dependent on Mitochondrial Activity

    doi: 10.3389/fmicb.2017.01423

    Figure Lengend Snippet: Effect of mitochondrial inhibitors during capsule induction. Cells from H99 strain were grown in Sabouraud liquid medium at 30°C (basal) and then transferred to 10% Sabouraud buffered at pH 7.3 with 50 mM MOPS in the presence of the highest concentration of antimycin A (3.6 μM) (A,B) , SHAM (4 mM) (C,D) , and rotenone (250 μM) (E,F) for 18 h. In each case, control samples incubated only with the solvent (EtOH or DMSO) or without any solvent (control) were carried out in parallel. After the incubation time, capsule size (A,C,E) was measured by suspending the cells in India ink as described in Section “Materials and Methods.” Asterisks indicate significant differences ( p

    Article Snippet: For this purpose, after the 48 h of incubation in the 96-well plates as described above, 10 μL from the wells of microdilution plates were placed on Sabouraud agar plates.

    Techniques: Concentration Assay, Incubation

    Effect of mitochondrial inhibitors on Cryptococcus neoformans . C. neoformans cells were cultured in liquid Sabouraud in presence of different metabolic inhibitors [sodium azide (A) , oligomycin (B) , antimycin A (C) , potassium cyanide (D) , salicylhydroxamic acid (E) , and rotenone (F) ] in 96-well plates and growth curves were performed at 48 or 72 h at 30°C. After this incubation time, 10 μL from the wells incubated with the inhibitor (I) or control carrying only the same concentration of solvent (C) were placed on Sabouraud agar plates and incubated at 30°C during 48 h. After that time, photographs were taken (right panels).

    Journal: Frontiers in Microbiology

    Article Title: Capsule Enlargement in Cryptococcus neoformans Is Dependent on Mitochondrial Activity

    doi: 10.3389/fmicb.2017.01423

    Figure Lengend Snippet: Effect of mitochondrial inhibitors on Cryptococcus neoformans . C. neoformans cells were cultured in liquid Sabouraud in presence of different metabolic inhibitors [sodium azide (A) , oligomycin (B) , antimycin A (C) , potassium cyanide (D) , salicylhydroxamic acid (E) , and rotenone (F) ] in 96-well plates and growth curves were performed at 48 or 72 h at 30°C. After this incubation time, 10 μL from the wells incubated with the inhibitor (I) or control carrying only the same concentration of solvent (C) were placed on Sabouraud agar plates and incubated at 30°C during 48 h. After that time, photographs were taken (right panels).

    Article Snippet: For this purpose, after the 48 h of incubation in the 96-well plates as described above, 10 μL from the wells of microdilution plates were placed on Sabouraud agar plates.

    Techniques: Cell Culture, Incubation, Concentration Assay

    Analysis of COX1 expression during capsule induction. Cells from H99 strain were grown in Sabouraud and capsule inducing medium at 37°C and RNA was isolated from samples were collected at different times (0, 3, 6, and 24 h). cDNAs were obtained and relative expression of the COX1 gene was measured by real-time PCR as described in Section “Materials and Methods.” COX1 gene expression at different time points in Sabouraud medium (A) and capsule inducing medium (B) . Then, a relative fold change comparing at each time point the difference between the capsule inducing medium and Sabouraud was plotted in (C) . This experiment was performed in triplicate.

    Journal: Frontiers in Microbiology

    Article Title: Capsule Enlargement in Cryptococcus neoformans Is Dependent on Mitochondrial Activity

    doi: 10.3389/fmicb.2017.01423

    Figure Lengend Snippet: Analysis of COX1 expression during capsule induction. Cells from H99 strain were grown in Sabouraud and capsule inducing medium at 37°C and RNA was isolated from samples were collected at different times (0, 3, 6, and 24 h). cDNAs were obtained and relative expression of the COX1 gene was measured by real-time PCR as described in Section “Materials and Methods.” COX1 gene expression at different time points in Sabouraud medium (A) and capsule inducing medium (B) . Then, a relative fold change comparing at each time point the difference between the capsule inducing medium and Sabouraud was plotted in (C) . This experiment was performed in triplicate.

    Article Snippet: For this purpose, after the 48 h of incubation in the 96-well plates as described above, 10 μL from the wells of microdilution plates were placed on Sabouraud agar plates.

    Techniques: Expressing, Isolation, Real-time Polymerase Chain Reaction

    Evaluation of mitochondrial membrane potential during capsular growth. Cells from H99 strain were incubated in capsule induction medium or Sabouraud for 0, 3, 6, and 24 h, and after each time, rhodamine 123 was added (see Materials and Methods). The uptake of this probe was analyzed by flow cytometry (left panel). The graph represents the results of a representative experiment, which was performed three different times. Cells in Sabouraud (Sab) are represented with histograms in black line and the cells in capsule inducing medium (MOPS) are represented in gray histograms. Representative cells from each time in the capsule inducing medium are shown in the right panels.

    Journal: Frontiers in Microbiology

    Article Title: Capsule Enlargement in Cryptococcus neoformans Is Dependent on Mitochondrial Activity

    doi: 10.3389/fmicb.2017.01423

    Figure Lengend Snippet: Evaluation of mitochondrial membrane potential during capsular growth. Cells from H99 strain were incubated in capsule induction medium or Sabouraud for 0, 3, 6, and 24 h, and after each time, rhodamine 123 was added (see Materials and Methods). The uptake of this probe was analyzed by flow cytometry (left panel). The graph represents the results of a representative experiment, which was performed three different times. Cells in Sabouraud (Sab) are represented with histograms in black line and the cells in capsule inducing medium (MOPS) are represented in gray histograms. Representative cells from each time in the capsule inducing medium are shown in the right panels.

    Article Snippet: For this purpose, after the 48 h of incubation in the 96-well plates as described above, 10 μL from the wells of microdilution plates were placed on Sabouraud agar plates.

    Techniques: Incubation, Flow Cytometry

    Production of ROS during capsule induction. Cells from H99 strain were grown in Sabouraud overnight then transfer to the same medium (control, black line) or capsule induction medium (induction, gray filled histogram). The production of ROS was evaluated at time 0, 3, 6, and 24 h by flow cytometry as described in Section “Materials and Methods.” As positive control we used samples in the presence of AmB (1 μg/mL). The graph represents the results of a representative experiment, which was performed three different times.

    Journal: Frontiers in Microbiology

    Article Title: Capsule Enlargement in Cryptococcus neoformans Is Dependent on Mitochondrial Activity

    doi: 10.3389/fmicb.2017.01423

    Figure Lengend Snippet: Production of ROS during capsule induction. Cells from H99 strain were grown in Sabouraud overnight then transfer to the same medium (control, black line) or capsule induction medium (induction, gray filled histogram). The production of ROS was evaluated at time 0, 3, 6, and 24 h by flow cytometry as described in Section “Materials and Methods.” As positive control we used samples in the presence of AmB (1 μg/mL). The graph represents the results of a representative experiment, which was performed three different times.

    Article Snippet: For this purpose, after the 48 h of incubation in the 96-well plates as described above, 10 μL from the wells of microdilution plates were placed on Sabouraud agar plates.

    Techniques: Flow Cytometry, Positive Control

    Validamycin A increases trehalose levels in Aspergillus flavus conidia with delayed conidial germination and decreased fungal adherence. (a) Aspergillus flavus ATCC 204304 was cultured at 37°C on Sabouraud dextrose agar for five days with or without 1 μ g/mL validamycin A. Trehalose assays were performed to measure trehalose levels in the conidia using glucose oxidase assays. Data are presented as means ± SE from three biological replicates. ∗∗∗ P value

    Journal: International Journal of Microbiology

    Article Title: The Inhibitory Effect of Validamycin A on Aspergillus flavus

    doi: 10.1155/2020/3972415

    Figure Lengend Snippet: Validamycin A increases trehalose levels in Aspergillus flavus conidia with delayed conidial germination and decreased fungal adherence. (a) Aspergillus flavus ATCC 204304 was cultured at 37°C on Sabouraud dextrose agar for five days with or without 1 μ g/mL validamycin A. Trehalose assays were performed to measure trehalose levels in the conidia using glucose oxidase assays. Data are presented as means ± SE from three biological replicates. ∗∗∗ P value

    Article Snippet: No significant difference was observed (one-way ANOVA with post hoc Bonferroni's test). (b) Aspergillus flavus ATCC-204304 and three clinical isolates were cultured at 37°C on Sabouraud dextrose agar for five days with or without 1 μ g/mL validamycin A. Trehalose assays were performed to measure trehalose levels in the conidia using glucose oxidase assays.

    Techniques: Cell Culture

    (Top) Isolate on Sabouraud dextrose agar. (Bottom) Wet prep (×100 magnification) of isolate showing budding yeast cells, arthroconidia, hyphae, and pseudohyphae.

    Journal: Journal of Clinical Microbiology

    Article Title: Photo Quiz: Bilateral Necrotizing Pneumonia in a 30-Year-Old Woman, a Hairy Situation

    doi: 10.1128/JCM.00451-20

    Figure Lengend Snippet: (Top) Isolate on Sabouraud dextrose agar. (Bottom) Wet prep (×100 magnification) of isolate showing budding yeast cells, arthroconidia, hyphae, and pseudohyphae.

    Article Snippet: The isolate was requested and received on a Sabouraud dextrose agar plate (Thermo Fisher Scientific Inc., Waltham, MA).

    Techniques: