Journal: The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien des Maladies Infectieuses et de la Microbiologie Médicale
Article Title: Chimeric Japanese Encephalitis Virus SA14/SA14-14-2 Was Virulence Attenuated and Protected the Challenge of Wild-Type Strain SA14
doi: 10.1155/2019/9179308
Figure Lengend Snippet: Identification of chimeric viruses JEV SA14/SA14-14-2 with the plaque assay and growth curves of the chimera in BHK21 cells: (a) plaque comparison of the chimera, SA14-14-2 and SA14; (b) growth curve of chimeric virus, JEV SA14/SA14-14-2 and SA14.
Article Snippet: pACNR-JEV SA14/SA14-14-2 was linearized with Xhol (NEB) and then coped with mung bean nuclease (NEB) to degrade the 3′ protruding end.
Techniques: Plaque Assay