Journal: Scientific Reports

Article Title: In-resin CLEM of Epon-embedded cells using proximity labeling

doi: 10.1038/s41598-022-15438-6

Figure Lengend Snippet: Fluorophore-conjugated streptavidin reacted with biotinylated proteins in cells after fixation with glutaraldehyde-containing mixture. ( A ) HeLa cells expressing miniTurbo ( A , upper panel) and mtActA-mTurbo fusion protein ( A , lower panel) were incubated in the presence of biotin (100, 300, and 500 μM) for the indicated times (10–160 min). After permeabilization, DyLight 549-streptavidin was reacted with the biotinylated proteins. Fluorescent images were obtained using a BZ-X810 fluorescence microscope (CCD monochrome camera, Nikon CFI Plan Apochromat × 40 lens, gain + 16 dB) using a filter set (excitation: 520–570 nm, dichroic mirror: 565 nm long pass, emission: 535–675 nm). ( B ) Fluorescent intensity in ( A ) was evaluated by ImageJ software. Error bars indicate the standard errors. Scale bar, 200 µm. * P value < 0.05, **** P value < 0.0001, ns not significant.

Article Snippet: After washing the cells with HB solution three times, the cells were incubated in HB solution containing 1 µg/ml of DyLight 549-conjugated streptavidin (VECTOR Lab, # SA-5549-1) at room temperature for 30 min.

Techniques: Expressing, Incubation, Fluorescence, Microscopy, Software

Journal: iScience

Article Title: Altered inhibitory function in hippocampal CA2 contributes in social memory deficits in Alzheimer’s mouse model

doi: 10.1016/j.isci.2022.103895

Figure Lengend Snippet:

Article Snippet: DyLight 549 Streptavidin antibody , Vector lab , Cat# SA-5549, RRID: AB_2336408.

Techniques: Plasmid Preparation, Recombinant, Software, Cell Counting