Journal: Neural Regeneration Research

Article Title: EZH2-dependent myelination following sciatic nerve injury

doi: 10.4103/NRR.NRR-D-23-02040

Figure Lengend Snippet: In situ hybridization spatial mRNA validation of Ezh2 expression in sciatic nerve of rats. (A) Schematic illustration of sciatic nerve samples obtained from normal, proximal, and distal segments of a rat sciatic nerve transection (SNT) model. Created with Adobe Illustrator CC and Adobe Photoshop CC. (B) Immunofluorescence staining of Schwann cells (SCs) on sciatic nerve or transected nerve probed for Ezh2 mRNA (green) at 4 days post-injury. All SCs were identified using S100B (Cy3, red), neurofilament 200 (NF200; Cy5, grey), and 4′,6-diamidino-2-phenylindole (DAPI; blue). Scale bars: 50 µm for low magnification, 20 µm for high magnification. (C) Percentage of SCs with Ezh2 mRNA ≥ 1 visible spots. (D) Fold change quantification of Ezh2 mRNA per SC. Ratio of number of average spots per SC were calculated for all groups, and then standardized to the normal sciatic nerve group value. SNT may induce expression of Ezh2 in SCs of both sciatic nerve stumps compared with the normal group. Data are expressed as mean ± SE ( n = 6). ** P < 0.01, vs . normal group (one-way analysis of variance followed by Bonferroni post hoc test).

Article Snippet: The primary antibodies used were: S100B antibody (polyclonal guinea pig, 1:1000, Synaptic Systems, Goettingen, Germany; Cat# 287004, RRID: AB_2620025), EZH2 antibody (rabbit, 1:1000, Active Motif, Carlsbad, CA, USA; Cat# 39902, RRID: AB_2614956), and MPZ antibody (chicken, 1:1000, Novus Biologicals Centennial, CO, USA; Cat# NB100-1607, RRID: AB_2144665).

Techniques: In Situ Hybridization, Expressing, Immunofluorescence, Staining

Journal: Neural Regeneration Research

Article Title: EZH2-dependent myelination following sciatic nerve injury

doi: 10.4103/NRR.NRR-D-23-02040

Figure Lengend Snippet: Ablation of Ezh2 in Schwann cells of mice leads to abnormal growth and myelination deficits in the sciatic nerve. (A) Diagram outlining the generation of Ezh2 conditional knockout ( Ezh2 fl/fl ; Mpz -Cre) mice. (B) Representative images of wild-type ( Ezh2 fl/fl ), heterozygous ( Ezh2 fl/+ ; Mpz -Cre), and homozygous conditional knockout mice ( Ezh2 fl/fl ; Mpz -Cre) at 7, 14, 21 days, and 8 weeks after birth. (C) Sciatic nerve without injury in wild-type control and Ezh2 fl/fl ; Mpz -Cre mice at 8 weeks post-birth stained for S100B (Cy5, grey), EZH2 (Cy3, red), MPZ (Alexa Fluor 488, green), and DAPI (blue). Genetic ablation of Ezh2 in Schwann cells leads to abnormal growth and deficits in the myelination of mouse sciatic nerve. Scale bars: 10 µm. DAPI: 4,6-Diamidino-2-phenylindole; MPZ: myelin protein zero.

Article Snippet: The primary antibodies used were: S100B antibody (polyclonal guinea pig, 1:1000, Synaptic Systems, Goettingen, Germany; Cat# 287004, RRID: AB_2620025), EZH2 antibody (rabbit, 1:1000, Active Motif, Carlsbad, CA, USA; Cat# 39902, RRID: AB_2614956), and MPZ antibody (chicken, 1:1000, Novus Biologicals Centennial, CO, USA; Cat# NB100-1607, RRID: AB_2144665).

Techniques: Knock-Out, Control, Staining

Journal: Neural Regeneration Research

Article Title: EZH2-dependent myelination following sciatic nerve injury

doi: 10.4103/NRR.NRR-D-23-02040

Figure Lengend Snippet: Knockout of Ezh2 in Schwann cells leads to hypomyelination of mouse sciatic nerve and suppresses remyelination post-SNC. (A) Transmission electron microscopy analysis of sciatic nerves from both Ezh2 cKO mice ( Ezh2 fl/fl ; Dhh -Cre and Ezh2 fl/fl ; Mpz -Cre) showed plenty of axons remain unmyelinated in mutant sciatic nerves. (B) Transmission electron microscopy analysis showed most axons remyelinated at the crush site of the control nerve while Ezh2 fl/fl ; Mpz -Cre mice had significantly fewer remyelinated axons than control nerve at 21 days after SNC. (C) Quantification of myelinated axons in sciatic nerves among both Ezh2 cKO mice and their wild-type littermate control mice at 8 weeks after birth and 21 days after SNC. Data are expressed as mean ± SE ( n = 3). * P < 0.05, *** P < 0.001, vs . wildtype control ( Ezh2 fl/fl ) group (one-way analysis of variance followed by Bonferroni post hoc test). (D) The percentage of myelinated axons in sciatic nerves among both Ezh2 cKO mice and wild-type control mice at 8 weeks after birth (P8W) and 21 days after SNC (21 dpi). (E) Sciatic nerve from wild-type control ( Ezh2 fl/fl ) and Ezh2 fl/fl ; Dhh -Cre P8W mice at 14 days post sciatic nerve crush (14 dpi) stained for S100B (Cy5, grey), EZH2 (Cy3, red), MPZ (Alexa Fluor 488, green), and DAPI (blue). Scale bars: 10 µm. cKO: Conditional knockout; DAPI: 4,6-diamidino-2-phenylindole; MPZ: myelin protein zero; SNC: sciatic nerve crush.

Article Snippet: The primary antibodies used were: S100B antibody (polyclonal guinea pig, 1:1000, Synaptic Systems, Goettingen, Germany; Cat# 287004, RRID: AB_2620025), EZH2 antibody (rabbit, 1:1000, Active Motif, Carlsbad, CA, USA; Cat# 39902, RRID: AB_2614956), and MPZ antibody (chicken, 1:1000, Novus Biologicals Centennial, CO, USA; Cat# NB100-1607, RRID: AB_2144665).

Techniques: Knock-Out, Transmission Assay, Electron Microscopy, Mutagenesis, Control, Staining

Journal: bioRxiv

Article Title: In vivo investigation of STN1 downregulation in melanoma formation in adult mice following UV irradiation

doi: 10.1101/2025.06.04.657974

Figure Lengend Snippet: (A) Scheme of TAM treatment and UV exposure. (B) IHC of Stn1 in skin tissue sections from Tyr::CreER T2 ; STN1 F/F and wild-type animals. Red arrows point to Stn1 staining in hair follicles of the control sample. Inserts show amplified images of the boxed areas. (C) Representative image of melanoma (dark speckles) developed on the back of the shaved skin in Tyr::CreER T2 ; Braf V600E mice. (D) Kaplan-Meier curve showing melanoma-free survival in UV-irradiated animals. (E) Representative H&E staining of skin tissues collected from mice. Inserts show amplified images of the boxed areas. (F) Representative IHC staining of S100B in skin tissues collected from mice. Inserts show amplified images of the boxed areas. Red arrows point to S100B positively stained cells. (G) Melanoma formation frequency in UV-irradiated animals. P : two-sided Fisher exact tests. The number of animals in each group is shown below the plot.

Article Snippet: Sections were then incubated with a primary antibody against Stn1 (1:100, Santa Cruz, 376450) and S100B (1:5,000, ProteinTech, 15146-AP) overnight at 4°C.

Techniques: Staining, Control, Amplification, Irradiation, Immunohistochemistry

Journal: PCN Reports: Psychiatry and Clinical Neurosciences

Article Title: Association between oxytocin and S100B in community‐dwelling older adults

doi: 10.1002/pcn5.70130

Figure Lengend Snippet: Correlation between serum oxytocin and S100B levels.

Article Snippet: Serum S100B levels were measured using ELISA, which was performed using a commercially available kit (EMD Millipore Corporation).

Techniques:

Journal: Cardiology Research

Article Title: Achieving Neuroprotection in the Setting of Early Extubation During Infant Cardiac Surgery: A Prospective, Randomized, and Blinded Study

doi: 10.14740/cr2029

Figure Lengend Snippet: Neuron-specific enolase (NSE) and S-100 protein beta chain (S100B).

Article Snippet: Plasma S100B levels were measured by colorimetric ELISA analysis using a commercially available kit (Bio Vendor R&D, Inc., Candler, NC) with included calibration controls.

Techniques: