s saprophyticus  (ATCC)

Journal: Scientific Reports

Article Title: Staphylococcus saprophyticus prevents skin damage by inhibiting Staphylococcus aureus quorum sensing

doi: 10.1038/s41598-025-97044-w

Figure Lengend Snippet: S. saprophyticus culture supernatant suppresses agr expression in S. aureus LAC strains. ( a – d ) S. aureus LAC psm α- lux ( a , c ) and P3- lux ( b , d ) reporter strains were grown for 8 h with 30% filtered spent media from S. chromogenes , S. simulans , S. saprophyticus , S. xylosus , S. capitis , S. lugdunensis , S. auricularis , and S. epidermidis cultures. Lux expression ( a , b ) and growth ( c , d ) in TSB after 8 h are shown. Data are mean ± SD from three independent experiments. n.s., not significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus TSB (–) according to one-way ANOVA with Tukey’s correction.

Article Snippet: However, Brown et al. recently reported that culture supernatant of the same S. saprophyticus strain (ATCC 15305) used in our experiments did not inhibit S. aureus agr expression.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Staphylococcus saprophyticus prevents skin damage by inhibiting Staphylococcus aureus quorum sensing

doi: 10.1038/s41598-025-97044-w

Figure Lengend Snippet: S. saprophyticus culture supernatant suppresses agr expression in S. aureus LAC strain over 24 h. ( a – d ) S. aureus LAC psm α- lux ( a , c ) and P3- lux ( b , d ) reporter strains were grown in 30% filtered spent media from S. saprophyticus and S. auricularis for 24 h. Growth ( a , b ) and lux expression ( c , d ) in TSB over 24 h are shown. Data are mean ± SD from three independent experiments. ** p < 0.01, **** p < 0.0001 versus TSB (–) according to one-way ANOVA test with Tukey’s correction.

Article Snippet: However, Brown et al. recently reported that culture supernatant of the same S. saprophyticus strain (ATCC 15305) used in our experiments did not inhibit S. aureus agr expression.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Staphylococcus saprophyticus prevents skin damage by inhibiting Staphylococcus aureus quorum sensing

doi: 10.1038/s41598-025-97044-w

Figure Lengend Snippet: S. saprophyticus culture supernatant inhibits Agr type-I and -II signaling in S. aureus. ( a – l ) S. aureus LAC (Agr type-I), HFH-30008 (Agr type-II), MW2 (Agr type-III), and HFH-30032 (Agr type-IV) strains were grown in 30% filtered spent media from S. saprophyticus and S. auricularis for 8 h. Growth ( a , d , g , j ) in TSB at 8 h and the expression of psm α ( b , e , h , k ) and RNAIII ( c , f , i , l ) after 8 h of growth as measured by qRT-PCR are shown. psm α and RNAIII expression levels are relative to that of the S. aureus housekeeping gene, gyrB. Data are mean ± SD from three independent experiments. n.s., not significant, * p < 0.05, ** p < 0.01 versus TSB (–) according to one-way ANOVA with Tukey’s correction.

Article Snippet: However, Brown et al. recently reported that culture supernatant of the same S. saprophyticus strain (ATCC 15305) used in our experiments did not inhibit S. aureus agr expression.

Techniques: Expressing, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Staphylococcus saprophyticus prevents skin damage by inhibiting Staphylococcus aureus quorum sensing

doi: 10.1038/s41598-025-97044-w

Figure Lengend Snippet: Synthetic S. saprophyticus AIP suppresses agr expression in the S. aureus LAC strain. ( a ) S. saprophyticus AIP structure. ( b – e ) S. aureus LAC psm α- lux ( b , d ) and P3- lux ( c , e ) reporter strains were cultured in TSB containing 1, 10, or 100 nM S. saprophyticus AIP for 24 h. Growth ( b , c ) and lux expression ( d , e ) over 24 h are shown. Data are mean ± SD from three independent experiments. n.s., not significant, * p < 0.05, ** p < 0.01 versus DMSO (–) according to one-way ANOVA with Tukey’s correction.

Article Snippet: However, Brown et al. recently reported that culture supernatant of the same S. saprophyticus strain (ATCC 15305) used in our experiments did not inhibit S. aureus agr expression.

Techniques: Expressing, Cell Culture

Journal: Scientific Reports

Article Title: Staphylococcus saprophyticus prevents skin damage by inhibiting Staphylococcus aureus quorum sensing

doi: 10.1038/s41598-025-97044-w

Figure Lengend Snippet: Synthetic S. saprophyticus AIP inhibits Agr type-I and -II signaling in S. aureus. ( a–l ) S. aureus LAC, HFH-30008, MW2, and HFH-30032 strains were incubated in TSB containing 1, 10, and 100 nM S. saprophyticus AIP for 8 h. Growth ( a – d ) and psm α ( e – h ) and RNAIII ( i – l ) expression as measured using qRT-PCR at 8 h are shown. Data are mean ± SD from three independent experiments. n.s., not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus DMSO (–) according to one-way ANOVA with Tukey’s correction.

Article Snippet: However, Brown et al. recently reported that culture supernatant of the same S. saprophyticus strain (ATCC 15305) used in our experiments did not inhibit S. aureus agr expression.

Techniques: Incubation, Expressing, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Staphylococcus saprophyticus prevents skin damage by inhibiting Staphylococcus aureus quorum sensing

doi: 10.1038/s41598-025-97044-w

Figure Lengend Snippet: S. aureus agr type-I expression is partially inhibited by the tail and thiolactone ring of S. saprophyticus AIP. ( a ) Structures of AIPs from S. aureus LAC (Agr type-I) and S. saprophyticus , and chimeric AIPs in which either the tail or the thiolactone ring of S. saprophyticus AIP was replaced with that of S. aureus. ( b – e ) S. aureus LAC psm α- lux ( b , d ) and P3- lux ( c , e ) reporter strains were incubated in TSB with increasing concentrations of the synthetic AIPs for 8 h. Data are mean ± SD from three independent experiments. **** p < 0.0001 versus S. aureus AIP according to one-way ANOVA with Tukey’s correction.

Article Snippet: However, Brown et al. recently reported that culture supernatant of the same S. saprophyticus strain (ATCC 15305) used in our experiments did not inhibit S. aureus agr expression.

Techniques: Expressing, Incubation

Journal: Scientific Reports

Article Title: Staphylococcus saprophyticus prevents skin damage by inhibiting Staphylococcus aureus quorum sensing

doi: 10.1038/s41598-025-97044-w

Figure Lengend Snippet: S. saprophyticus AIP protects against skin damage induced by epidermal colonization with S. aureus . ( a – c ) Representative macroscopic and hematoxylin and eosin-stained microscopic images ( a ), skin disease scores ( b ), and S. aureus CFU in the skin ( c ) of mice epicutaneously colonized with S. aureus LAC WT strain containing DMSO (–) or S. saprophyticus AIP (50 μg) at 6 days post-infection (n = 5). Findings for mice colonized with S. aureus LAC Δagr are shown for comparison. In b and c , each dot represents a mouse. Data are representative of three independent experiments. n.s., not significant, **** p < 0.0001 according to one-way ANOVA with Tukey’s correction.

Article Snippet: However, Brown et al. recently reported that culture supernatant of the same S. saprophyticus strain (ATCC 15305) used in our experiments did not inhibit S. aureus agr expression.

Techniques: Staining, Infection, Comparison

Journal: Scientific Reports

Article Title: Staphylococcus saprophyticus prevents skin damage by inhibiting Staphylococcus aureus quorum sensing

doi: 10.1038/s41598-025-97044-w

Figure Lengend Snippet: S. saprophyticus AIP protects skin damage by intradermal inoculation with S. aureus . ( a – c ) Representative macroscopic and hematoxylin and eosin-stained microscopic images ( a ), skin lesion sizes at the indicated time points ( b ), and S. aureus CFU in the skin ( c ) of mice intradermally inoculated with S. aureus LAC WT strain containing DMSO (–) or S. saprophyticus AIP (250 μg) at 6 days post-infection (n = 5). Findings for mice colonized with S. aureus LAC Δagr are shown for comparison. In c, each dot represents a mouse. Data are representative of three independent experiments. n.s., not significant, **** p < 0.0001 according to one-way ANOVA with Tukey’s correction.

Article Snippet: However, Brown et al. recently reported that culture supernatant of the same S. saprophyticus strain (ATCC 15305) used in our experiments did not inhibit S. aureus agr expression.

Techniques: Staining, Infection, Comparison

Journal: Virology Journal

Article Title: Isolation, characterization, and genomic analysis of three novel Herelleviridae family lytic bacteriophages against uropathogenic isolates of Staphylococcus saprophyticus

doi: 10.1186/s12985-025-02710-0

Figure Lengend Snippet: Host spectrum of bacteriophages against clinical isolates of Staphylococcus saprophyticus

Article Snippet: Subsequently, the DNA of each S. saprophyticus isolate intended for phage identification was extracted using a commercial gram-positive bacterial kit (SinaPure, EX6021, SinaClon, Iran).

Techniques:

Journal: Virology Journal

Article Title: Isolation, characterization, and genomic analysis of three novel Herelleviridae family lytic bacteriophages against uropathogenic isolates of Staphylococcus saprophyticus

doi: 10.1186/s12985-025-02710-0

Figure Lengend Snippet: Electron micrographs of phages that infect S. saprophyticus . Newly identified phages were named vB_SsapH-Golestan100 ( A ), vB_SsapH-Golestan101-M ( B ), and vB_SsapH-Golestan105-M ( C ).

Article Snippet: Subsequently, the DNA of each S. saprophyticus isolate intended for phage identification was extracted using a commercial gram-positive bacterial kit (SinaPure, EX6021, SinaClon, Iran).

Techniques: