s saprophyticus ag1  (ATCC)


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    ATCC s saprophyticus ag1
    Microorganisms used in this work.
    S Saprophyticus Ag1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    s saprophyticus ag1 - by Bioz Stars, 2024-04
    86/100 stars

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    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

    s saprophyticus ag1 cells  (ATCC)


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    Structured Review

    ATCC s saprophyticus ag1 cells
    Microorganisms used in this work.
    S Saprophyticus Ag1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s saprophyticus ag1 cells/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s saprophyticus ag1 cells - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

    s saprophyticus ag1 biofilm  (ATCC)


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    Structured Review

    ATCC s saprophyticus ag1 biofilm
    Microorganisms used in this work.
    S Saprophyticus Ag1 Biofilm, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s saprophyticus ag1 biofilm/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s saprophyticus ag1 biofilm - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

    s saprophyticus ag1  (ATCC)


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    ATCC s saprophyticus ag1
    Microorganisms used in this work.
    S Saprophyticus Ag1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

    s saprophyticus ag1 cells  (ATCC)


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    ATCC s saprophyticus ag1 cells
    Microorganisms used in this work.
    S Saprophyticus Ag1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

    s saprophyticus ag1  (ATCC)


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    ATCC s saprophyticus ag1
    Microorganisms used in this work.
    S Saprophyticus Ag1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s saprophyticus ag1/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    86/100 stars

    Images

    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

    s saprophyticus ag1  (ATCC)


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    ATCC s saprophyticus ag1
    Microorganisms used in this work.
    S Saprophyticus Ag1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

    s saprophyticus ag1  (ATCC)


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    Structured Review

    ATCC s saprophyticus ag1
    Microorganisms used in this work.
    S Saprophyticus Ag1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s saprophyticus ag1/product/ATCC
    Average 86 stars, based on 1 article reviews
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    Images

    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

    s saprophyticus ag1 planktonic cells  (ATCC)


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    ATCC s saprophyticus ag1 planktonic cells
    Microorganisms used in this work.
    S Saprophyticus Ag1 Planktonic Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s saprophyticus ag1 planktonic cells/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s saprophyticus ag1 planktonic cells - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

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    ATCC s saprophyticus ag1 planktonic cells
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    1) Product Images from "Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm"

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2024.e28811

    Microorganisms used in this work.
    Figure Legend Snippet: Microorganisms used in this work.

    Techniques Used: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.
    Figure Legend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Techniques Used:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Incubation, Irradiation

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    ATCC s saprophyticus ag1
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    Microorganisms used in this work.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Microorganisms used in this work.

    Article Snippet: For the RF- and Chl-mediated photoinactivation of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1, their cells were resuspended in 0.011 mM RF (pH 7.4) or 0.015 mM Chl (pH 7.4) to a 10 7 CFU/mL concentration.

    Techniques: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Article Snippet: For the RF- and Chl-mediated photoinactivation of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1, their cells were resuspended in 0.011 mM RF (pH 7.4) or 0.015 mM Chl (pH 7.4) to a 10 7 CFU/mL concentration.

    Techniques:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Article Snippet: For the RF- and Chl-mediated photoinactivation of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1, their cells were resuspended in 0.011 mM RF (pH 7.4) or 0.015 mM Chl (pH 7.4) to a 10 7 CFU/mL concentration.

    Techniques:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: For the RF- and Chl-mediated photoinactivation of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1, their cells were resuspended in 0.011 mM RF (pH 7.4) or 0.015 mM Chl (pH 7.4) to a 10 7 CFU/mL concentration.

    Techniques: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: For the RF- and Chl-mediated photoinactivation of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1, their cells were resuspended in 0.011 mM RF (pH 7.4) or 0.015 mM Chl (pH 7.4) to a 10 7 CFU/mL concentration.

    Techniques: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: For the RF- and Chl-mediated photoinactivation of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1, their cells were resuspended in 0.011 mM RF (pH 7.4) or 0.015 mM Chl (pH 7.4) to a 10 7 CFU/mL concentration.

    Techniques: Incubation, Irradiation

    Microorganisms used in this work.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Microorganisms used in this work.

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques: Incubation, Irradiation

    Microorganisms used in this work.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Microorganisms used in this work.

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Planktonic S. epidermidis ATCC 12228 and S. saprophyticus AG1 cells and S. saprophyticus AG1 biofilm were photoinactivated as described in Sections , .2.

    Techniques: Incubation, Irradiation

    Microorganisms used in this work.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Microorganisms used in this work.

    Article Snippet: During RF-API, with the increase of the irradiation dose, the ability of the S. saprophyticus AG1 planktonic cells to reduce alamarBlue™ decreased (as in the case of S. epidermidis ATCC 12228 planktonic cells study ( , C).

    Techniques: Isolation

    (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: (A) RF-API and ( B) Chl-API of the planktonic cells of S. epidermidis ATCC 12228; ( C ) RF-API and ( D ) Chl-API of the planktonic cells of S. saprophyticus AG1. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the bactericidal effect. ns – non-significant; + - the points are below the detection limit. ( E ) CFU counts of dark controls (reported in log 10 scale) of the planktonic cells of S. epidermidis ATCC 12228 and S. saprophyticus AG1.* - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Article Snippet: During RF-API, with the increase of the irradiation dose, the ability of the S. saprophyticus AG1 planktonic cells to reduce alamarBlue™ decreased (as in the case of S. epidermidis ATCC 12228 planktonic cells study ( , C).

    Techniques:

    RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: RF-API ( A ) and Chl-API ( B ) of S. saprophyticus AG1 biofilm. A horizontal dashed line in the graphs denotes minimal 3 log 10 reduction (indicating bactericidal effect). Black circles on the x axis indicate the minimal illumination doses required for the minimal 3 log 10 bactericidal effect of the tested bacterial biofilms. ns – non-significant. ( C ) CFU counts of dark controls (reported in log 10 scale) of the studied S. saprophyticus AG1 biofilms. * - dark control with RF or Chl, respectively; ** - dark control without RF or Chl, respectively; n – was not evaluated; +RF+440 nm vs + RF-440 nm and –RF-440 nm or + Chl+402 nm vs + Chl-402 nm and –Chl-402 nm p value < 0.05.

    Article Snippet: During RF-API, with the increase of the irradiation dose, the ability of the S. saprophyticus AG1 planktonic cells to reduce alamarBlue™ decreased (as in the case of S. epidermidis ATCC 12228 planktonic cells study ( , C).

    Techniques:

    Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Ability of the planktonic cells of the S. saprophyticus AG1 to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm ( A )/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - cells irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs dark control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: During RF-API, with the increase of the irradiation dose, the ability of the S. saprophyticus AG1 planktonic cells to reduce alamarBlue™ decreased (as in the case of S. epidermidis ATCC 12228 planktonic cells study ( , C).

    Techniques: Irradiation

    Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: Ability of S. saprophyticus AG1 biofilms to reduce alamarBlue™ after Chl- (402 nm at 5 mW/m 2 using appropriate doses) and RF-API (440 nm at 5 mW/m 2 using appropriate doses). Designations: +Chl +402 nm (A)/-Chl +402 nm ( B )/+RF +440 nm ( C )/–RF +440 nm ( D ) - biofilms irradiated by 402 nm/440 nm with/without 0.015 mM Chl/0.011 mM RF, respectively. Green ( A ), orange ( C ) and black ( B , D ) dashed lines show the changes in the counts of CFU during irradiation with and without Chl ( A , B ) or RF ( C , D ), respectively. +RF +440 nm/+Chl +402 nm vs control groups (+Chl – 402 nm/+RF-440 nm; -Chl −402 nm/-RF-440 nm): p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: During RF-API, with the increase of the irradiation dose, the ability of the S. saprophyticus AG1 planktonic cells to reduce alamarBlue™ decreased (as in the case of S. epidermidis ATCC 12228 planktonic cells study ( , C).

    Techniques: Irradiation

    SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Heliyon

    Article Title: Evaluation of visible light and natural photosensitizers against Staphylococcus epidermidis and Staphylococcus saprophyticus planktonic cells and biofilm

    doi: 10.1016/j.heliyon.2024.e28811

    Figure Lengend Snippet: SEM images of S. saprophyticus AG1 biofilm: ( A ) mechanically detached biofilms from a greater distance; ( B ) untreated (control) biofilm (-PS- hV ) - no PSs (RF nor Chl) in dark conditions) – incubated for an amount of minutes corresponding to 140 J/cm 2 ; ( C ) biofilm after + Chl+402 nm (treated with 0.015 mM Chl and irradiated by 402 nm up to 75 J/cm 2 – Chl-API dose at which bactericidal effect was determined); ( D ) biofilm after -Chl+402 nm (not treated with 0.015 mM Chl prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) instead and irradiated by 402 nm up to 75 J/cm 2 ); ( E ) biofilm after + RF+440 nm (treated with 0.011 mM RF and irradiated by 440 nm up to 140 J/cm 2 - RF-API dose at which bactericidal effect was determined); ( F ) biofilm after -RF+440 nm (not treated with 0.011 mM RF prior to irradiation, cells were covered with 0.1 M phosphate buffer (pH 7.4) and irradiated by 440 nm up to 140 J/cm 2 ). Yellow arrows indicate lysed and sloughed, dead staphylococcal biofilm-forming cells. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: During RF-API, with the increase of the irradiation dose, the ability of the S. saprophyticus AG1 planktonic cells to reduce alamarBlue™ decreased (as in the case of S. epidermidis ATCC 12228 planktonic cells study ( , C).

    Techniques: Incubation, Irradiation