s aureus atcc 25923  (ATCC)


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    Name:
    Staphylococcus aureus subsp aureus
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    Catalog Number:
    25923
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    ATCC s aureus atcc 25923
    Time-kill curves of S. aureus <t>ATCC</t> 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.

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    Images

    1) Product Images from "Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland"

    Article Title: Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23020260

    Time-kill curves of S. aureus ATCC 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.
    Figure Legend Snippet: Time-kill curves of S. aureus ATCC 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.

    Techniques Used:

    2) Product Images from "Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland"

    Article Title: Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23020260

    Time-kill curves of S. aureus ATCC 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.
    Figure Legend Snippet: Time-kill curves of S. aureus ATCC 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.

    Techniques Used:

    3) Product Images from "Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria"

    Article Title: Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-18590-6

    Antibacterial activity of AgNPs against different bacteria ( E .  coli  ATCC 25922,  S. aureus  ATCC 25923,  B. subtilis ,  P. aeruginosa ,  K. pneumoniae  AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.
    Figure Legend Snippet: Antibacterial activity of AgNPs against different bacteria ( E . coli ATCC 25922, S. aureus ATCC 25923, B. subtilis , P. aeruginosa , K. pneumoniae AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.

    Techniques Used: Activity Assay

    4) Product Images from "Discrepancy between Uptake and Intracellular Activity of Moxifloxacin in a Staphylococcus aureus-Human THP-1 Monocytic Cell Model"

    Article Title: Discrepancy between Uptake and Intracellular Activity of Moxifloxacin in a Staphylococcus aureus-Human THP-1 Monocytic Cell Model

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.46.2.288-293.2002

    Influence of moxifloxacin on the viability of S. aureus ATCC 25923 using inocula of 10 5 (A) and 10 7 CFU/ml (B). The antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in two determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter; ×, 0.1 mg/liter ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.
    Figure Legend Snippet: Influence of moxifloxacin on the viability of S. aureus ATCC 25923 using inocula of 10 5 (A) and 10 7 CFU/ml (B). The antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in two determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter; ×, 0.1 mg/liter ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.

    Techniques Used:

    Effect of moxifloxacin on the viability of S. aureus ATCC 25923 ingested by THP-1 cells. Antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in three determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter, ×, 0.1 mg/liter; ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.
    Figure Legend Snippet: Effect of moxifloxacin on the viability of S. aureus ATCC 25923 ingested by THP-1 cells. Antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in three determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter, ×, 0.1 mg/liter; ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.

    Techniques Used:

    5) Product Images from "Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils"

    Article Title: Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils

    Journal: Materials

    doi: 10.3390/ma13071618

    Minimal concentration for biofilm eradication (MCBE) of collagen nanofibres loaded with essential oils against  S. aureus  ATCC 25923, as compared to gentamicin and essential oils.
    Figure Legend Snippet: Minimal concentration for biofilm eradication (MCBE) of collagen nanofibres loaded with essential oils against S. aureus ATCC 25923, as compared to gentamicin and essential oils.

    Techniques Used: Concentration Assay

    Minimum inhibitory concentration (MIC) of collagen nanofibres loaded with essential oils against  S. aureus  ATCC 25923, as compared to gentamicin and essential oils.
    Figure Legend Snippet: Minimum inhibitory concentration (MIC) of collagen nanofibres loaded with essential oils against S. aureus ATCC 25923, as compared to gentamicin and essential oils.

    Techniques Used: Concentration Assay

    6) Product Images from "Contrasting Effects of Acidic pH on the Extracellular and Intracellular Activities of the Anti-Gram-Positive Fluoroquinolones Moxifloxacin and Delafloxacin against Staphylococcus aureus ▿ ▿ †"

    Article Title: Contrasting Effects of Acidic pH on the Extracellular and Intracellular Activities of the Anti-Gram-Positive Fluoroquinolones Moxifloxacin and Delafloxacin against Staphylococcus aureus ▿ ▿ †

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.01201-10

    Accumulation in S. aureus ATCC 25923 (left) or MIC (right) of fluoroquinolones in broth at different pHs. Left: growing bacteria were incubated for 30 min in pH-adjusted broth with delafloxacin (100 mg/liter) or moxifloxacin (50 mg/liter). Values are
    Figure Legend Snippet: Accumulation in S. aureus ATCC 25923 (left) or MIC (right) of fluoroquinolones in broth at different pHs. Left: growing bacteria were incubated for 30 min in pH-adjusted broth with delafloxacin (100 mg/liter) or moxifloxacin (50 mg/liter). Values are

    Techniques Used: Incubation

    Activities of delafloxacin and moxifloxacin against S. aureus ATCC 25923 after 24 h of incubation in broth at pH 5.5 or 7.4. Top: change in CFU from time zero (log scale) for 4 selected concentrations. Limit of detection is set at −5 log CFU.
    Figure Legend Snippet: Activities of delafloxacin and moxifloxacin against S. aureus ATCC 25923 after 24 h of incubation in broth at pH 5.5 or 7.4. Top: change in CFU from time zero (log scale) for 4 selected concentrations. Limit of detection is set at −5 log CFU.

    Techniques Used: Incubation

    Dose-response activities of delafloxacin and moxifloxacin against the intracellular forms of S. aureus strain ATCC 25923 (THP-1 macrophages). Cells were incubated with increasing concentrations of antibiotic (total drug) for 24 h in RPMI 1640 medium adjusted
    Figure Legend Snippet: Dose-response activities of delafloxacin and moxifloxacin against the intracellular forms of S. aureus strain ATCC 25923 (THP-1 macrophages). Cells were incubated with increasing concentrations of antibiotic (total drug) for 24 h in RPMI 1640 medium adjusted

    Techniques Used: Incubation

    7) Product Images from "Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland"

    Article Title: Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23020260

    Time-kill curves of S. aureus ATCC 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.
    Figure Legend Snippet: Time-kill curves of S. aureus ATCC 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.

    Techniques Used:

    8) Product Images from "Design, Recombinant Fusion Expression and Biological Evaluation of Vasoactive Intestinal Peptide Analogue as Novel Antimicrobial Agent"

    Article Title: Design, Recombinant Fusion Expression and Biological Evaluation of Vasoactive Intestinal Peptide Analogue as Novel Antimicrobial Agent

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules22111963

    Antimicrobial mechanism of VIP and the VIP analogue 8. Transmission electron micrographs of  E. coli  ATCC 25922; Transmission electron micrographs of  S. aureus  ATCC 25923; bacteria in mid-logarithmic growth were treated with peptides at 1 × MIC for 2 h.
    Figure Legend Snippet: Antimicrobial mechanism of VIP and the VIP analogue 8. Transmission electron micrographs of E. coli ATCC 25922; Transmission electron micrographs of S. aureus ATCC 25923; bacteria in mid-logarithmic growth were treated with peptides at 1 × MIC for 2 h.

    Techniques Used: Transmission Assay

    9) Product Images from "Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs"

    Article Title: Susceptibility of Staphylococcus aureus Clinical Isolates to Propolis Extract Alone or in Combination with Antimicrobial Drugs

    Journal: Molecules

    doi: 10.3390/molecules18089623

    Inhibitory effect of 10 antimicrobial agents alone, and in combination with EEPP on 12  Staphylococcus  strains evaluated by disk diffusion method. ( A ) FOX and FOX+EEPP; ( B ) DA and DA+EEPP; ( C ) E and E+EEPP; ( D ) CIP and CIP+EEPP; ( E ) TE and TE+EEPP; ( F ) P and P+EEPP; ( G ) TOB and TOB+EEPP; ( H ) LIN and LIN+EEPP; ( I ) C and C+EEPP; ( J ) STX and STX+EEPP. MHA: Mueller-Hinton Agar; MHA with EEPP: MHA plus one-fourth of MIC90 of EEPP; blue bars: diameters of the growth inhibition zones (in mm) for antibiotic alone; red bars: diameters of the growth inhibition zones (in mm) for combined effect of antibiotics and EEPP; FOX: Cefoxitin; DA: Clindamycin; E: Erythromycin; CIP: Ciprofloxacin; TE: Tetracycline; P: Penicillin; TOB: Tobramycin; LIN: Linezolid; C: Chloramphenicol; STX: Trimethoprim+Sulfamethoxazole; K1:  S. aureus  ATCC 25923; K2:  S. aureus  MRSA ATCC 43300; * Wilcoxon Signed-Rank Test, statistical significant level at  p
    Figure Legend Snippet: Inhibitory effect of 10 antimicrobial agents alone, and in combination with EEPP on 12 Staphylococcus strains evaluated by disk diffusion method. ( A ) FOX and FOX+EEPP; ( B ) DA and DA+EEPP; ( C ) E and E+EEPP; ( D ) CIP and CIP+EEPP; ( E ) TE and TE+EEPP; ( F ) P and P+EEPP; ( G ) TOB and TOB+EEPP; ( H ) LIN and LIN+EEPP; ( I ) C and C+EEPP; ( J ) STX and STX+EEPP. MHA: Mueller-Hinton Agar; MHA with EEPP: MHA plus one-fourth of MIC90 of EEPP; blue bars: diameters of the growth inhibition zones (in mm) for antibiotic alone; red bars: diameters of the growth inhibition zones (in mm) for combined effect of antibiotics and EEPP; FOX: Cefoxitin; DA: Clindamycin; E: Erythromycin; CIP: Ciprofloxacin; TE: Tetracycline; P: Penicillin; TOB: Tobramycin; LIN: Linezolid; C: Chloramphenicol; STX: Trimethoprim+Sulfamethoxazole; K1: S. aureus ATCC 25923; K2: S. aureus MRSA ATCC 43300; * Wilcoxon Signed-Rank Test, statistical significant level at p

    Techniques Used: Diffusion-based Assay, Inhibition

    Identification of  mecA  gene fragment (533 bp). W1— S. aureus  MSSA ATCC 25923; W2— S. aureus  MRSA ATCC 43300; 1–10  S. aureus  clinical isolates; M-100–1000 bp marker, (+) positive control, (−) negative control.
    Figure Legend Snippet: Identification of mecA gene fragment (533 bp). W1— S. aureus MSSA ATCC 25923; W2— S. aureus MRSA ATCC 43300; 1–10 S. aureus clinical isolates; M-100–1000 bp marker, (+) positive control, (−) negative control.

    Techniques Used: Marker, Positive Control, Negative Control

    10) Product Images from "Transcriptional and Functional Analysis of the Effects of Magnolol: Inhibition of Autolysis and Biofilms in Staphylococcus aureus"

    Article Title: Transcriptional and Functional Analysis of the Effects of Magnolol: Inhibition of Autolysis and Biofilms in Staphylococcus aureus

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0026833

    Zymographic analysis of bacteriolytic hydrolase activities of S. aureus ATCC 25923 cells treated with MOL. LiCl (A) and SDS (B) autolysin extracts containing S. aureus ATCC 25923 cell walls treated with various concentrations of MOL. Lane 1, no MOL treatment; lane 2, 1/4× MIC MOL; lane 3, 1/2× MIC MOL; lane 4, 1× MIC MOL. Molecular size markers are indicated on the left. The data shown are from a single representative experiment and were reproduced several times.
    Figure Legend Snippet: Zymographic analysis of bacteriolytic hydrolase activities of S. aureus ATCC 25923 cells treated with MOL. LiCl (A) and SDS (B) autolysin extracts containing S. aureus ATCC 25923 cell walls treated with various concentrations of MOL. Lane 1, no MOL treatment; lane 2, 1/4× MIC MOL; lane 3, 1/2× MIC MOL; lane 4, 1× MIC MOL. Molecular size markers are indicated on the left. The data shown are from a single representative experiment and were reproduced several times.

    Techniques Used:

    Effect of MOL on Triton X-100-induced autolysis. Triton X-100 was used to stimulate autolysis in S. aureus ATCC 25923 cells grown in the absence or presence of various concentrations of MOL. The four concentration investigated were *, 2× MIC; ▴, 1× MIC; △, 1/2× MIC; □, 1/4× MIC; ⧫, untreated. The data were from a single representative experiment and were reproduced several times.
    Figure Legend Snippet: Effect of MOL on Triton X-100-induced autolysis. Triton X-100 was used to stimulate autolysis in S. aureus ATCC 25923 cells grown in the absence or presence of various concentrations of MOL. The four concentration investigated were *, 2× MIC; ▴, 1× MIC; △, 1/2× MIC; □, 1/4× MIC; ⧫, untreated. The data were from a single representative experiment and were reproduced several times.

    Techniques Used: Concentration Assay

    Role of MOL on DNA release of S. aureus . The amount of eDNA in the cell-free supernatants from the S. aureus strain ATCC 25923 biofilms treated with MOL at concentration of 1/16∼8× MIC was measured by spectrophotometry. The values are expressed as nanogram of eDNA per relative biofilm biomass (OD600). Values represent the mean ± SD for three independent experiments. ** represents p
    Figure Legend Snippet: Role of MOL on DNA release of S. aureus . The amount of eDNA in the cell-free supernatants from the S. aureus strain ATCC 25923 biofilms treated with MOL at concentration of 1/16∼8× MIC was measured by spectrophotometry. The values are expressed as nanogram of eDNA per relative biofilm biomass (OD600). Values represent the mean ± SD for three independent experiments. ** represents p

    Techniques Used: Concentration Assay, Spectrophotometry

    Confocal laser scanning microscopy image of LIVE/DEAD®-stained illustrating the effects of different MOL concentrations on established S. aureus ATCC 25923 biofilm formation. Biofilms were formed on coverslides within 48 h at 37°C. Established biofilms were treated with MOL at 128 µg/mL, 256 µg/mL, or 512 µg/mL for 48 h at 37°C. (A) Control (untreated); (B to D) treatment with MOL at 128 µg/mL, 256 µg/mL, or 512 µg/mL respectively. Green, viable cells; Red, dead cells.
    Figure Legend Snippet: Confocal laser scanning microscopy image of LIVE/DEAD®-stained illustrating the effects of different MOL concentrations on established S. aureus ATCC 25923 biofilm formation. Biofilms were formed on coverslides within 48 h at 37°C. Established biofilms were treated with MOL at 128 µg/mL, 256 µg/mL, or 512 µg/mL for 48 h at 37°C. (A) Control (untreated); (B to D) treatment with MOL at 128 µg/mL, 256 µg/mL, or 512 µg/mL respectively. Green, viable cells; Red, dead cells.

    Techniques Used: Confocal Laser Scanning Microscopy, Staining

    Quantitative analysis of extracellular bacteriolytic hydrolase activities in S. aureus ATCC 25923 cells treated with various concentration of MOL. The four concentration investigated were *, 2× MIC; □, 1× MIC; ▴, 1/2× MIC; ▪, 1/4× MIC; ⧫, untreated. The data were from a single representative experiment and were reproduced several times.
    Figure Legend Snippet: Quantitative analysis of extracellular bacteriolytic hydrolase activities in S. aureus ATCC 25923 cells treated with various concentration of MOL. The four concentration investigated were *, 2× MIC; □, 1× MIC; ▴, 1/2× MIC; ▪, 1/4× MIC; ⧫, untreated. The data were from a single representative experiment and were reproduced several times.

    Techniques Used: Concentration Assay

    11) Product Images from "Antimicrobial Fatty Acids from Green Alga Ulva rigida (Chlorophyta)"

    Article Title: Antimicrobial Fatty Acids from Green Alga Ulva rigida (Chlorophyta)

    Journal: BioMed Research International

    doi: 10.1155/2018/3069595

    Antibacterial activity of  U. rigida  G4, G5, and G6 fractions and subfractions against  S. aureus  ATCC 25923.
    Figure Legend Snippet: Antibacterial activity of U. rigida G4, G5, and G6 fractions and subfractions against S. aureus ATCC 25923.

    Techniques Used: Activity Assay

    12) Product Images from "Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus"

    Article Title: Transcriptional and Functional Analysis Shows Sodium Houttuyfonate-Mediated Inhibition of Autolysis in Staphylococcus aureus

    Journal: Molecules

    doi: 10.3390/molecules16108848

    Zymographic analysis of bacteriolytic hydrolase activities of S. aureus ATCC 25923 cells treated with SH. LiCl ( A ) and SDS ( B ) autolysin extracts containing S. aureus ATCC 25923 cell walls treated with various concentrations of SH. Lane 1, no SH treatment; lane 2, 1/4× MIC SH; lane 3, 1/2× MIC SH; lane 4, 1× MIC SH. Molecular size markers are indicated on the left. The data shown are from a single representative experiment and were reproduced several times.
    Figure Legend Snippet: Zymographic analysis of bacteriolytic hydrolase activities of S. aureus ATCC 25923 cells treated with SH. LiCl ( A ) and SDS ( B ) autolysin extracts containing S. aureus ATCC 25923 cell walls treated with various concentrations of SH. Lane 1, no SH treatment; lane 2, 1/4× MIC SH; lane 3, 1/2× MIC SH; lane 4, 1× MIC SH. Molecular size markers are indicated on the left. The data shown are from a single representative experiment and were reproduced several times.

    Techniques Used:

    Role of SH on DNA release of S. aureus . The amount of eDNA stained by PI in S. aureus strain ATCC 25923 cultures treated with SH at concentration of 1/16× MIC, 1/8× MIC, 1/4× MIC, 1/2× MIC, 1× MIC and 2× MIC, 4× MIC, 8× MIC was measured. The drug-free culture was used as calibrator. Values represent the mean ± SD for three independent experiments. * represents p
    Figure Legend Snippet: Role of SH on DNA release of S. aureus . The amount of eDNA stained by PI in S. aureus strain ATCC 25923 cultures treated with SH at concentration of 1/16× MIC, 1/8× MIC, 1/4× MIC, 1/2× MIC, 1× MIC and 2× MIC, 4× MIC, 8× MIC was measured. The drug-free culture was used as calibrator. Values represent the mean ± SD for three independent experiments. * represents p

    Techniques Used: Staining, Concentration Assay

    Quantitative analysis of extracellular bacteriolytic hydrolase activities in S. aureus ATCC 25923 cells treated with various concentration of SH. The four concentration investigated were □, 2× MIC; ▲, MIC; *, 1/2 MIC; △, 1/4 MIC; ◆, untreated.
    Figure Legend Snippet: Quantitative analysis of extracellular bacteriolytic hydrolase activities in S. aureus ATCC 25923 cells treated with various concentration of SH. The four concentration investigated were □, 2× MIC; ▲, MIC; *, 1/2 MIC; △, 1/4 MIC; ◆, untreated.

    Techniques Used: Concentration Assay

    Effect of SH on Triton X-100-induced autolysis. Triton X-100 was used to stimulate autolysis in S. aureus ATCC 25923 cells grown in the absence or presence of various concentration of SH. The four concentration investigated were: ▲, 2× MIC; *, MIC; △, 1/2 MIC; □, 1/4 MIC; ◆, untreated. Data points are the mean ± SD of the mean of three replicate samples. * represents P
    Figure Legend Snippet: Effect of SH on Triton X-100-induced autolysis. Triton X-100 was used to stimulate autolysis in S. aureus ATCC 25923 cells grown in the absence or presence of various concentration of SH. The four concentration investigated were: ▲, 2× MIC; *, MIC; △, 1/2 MIC; □, 1/4 MIC; ◆, untreated. Data points are the mean ± SD of the mean of three replicate samples. * represents P

    Techniques Used: Concentration Assay

    Growth curve for S. aureus strain ATCC 25923 in the presence or absence of SH. ◆, untreated S. aureus ; □, S. aureus plus 4 µg/mL SH; ▲, S. aureus plus 8 µg/mL SH; ■, S. aureus plus 16 µg/mL SH; and *, S. aureus plus 32 µg/mL SH; △, S. aureus plus 64 µg/mL SH.
    Figure Legend Snippet: Growth curve for S. aureus strain ATCC 25923 in the presence or absence of SH. ◆, untreated S. aureus ; □, S. aureus plus 4 µg/mL SH; ▲, S. aureus plus 8 µg/mL SH; ■, S. aureus plus 16 µg/mL SH; and *, S. aureus plus 32 µg/mL SH; △, S. aureus plus 64 µg/mL SH.

    Techniques Used:

    13) Product Images from "The Inhibitory Potential of Thai Mango Seed Kernel Extract against Methicillin-Resistant Staphylococcus Aureus"

    Article Title: The Inhibitory Potential of Thai Mango Seed Kernel Extract against Methicillin-Resistant Staphylococcus Aureus

    Journal: Molecules

    doi: 10.3390/molecules16086255

    Transmission electron micrographs of S. aureus ATCC 25923 ( A –D ) and the M09 MRSA strain ( E –H ) 12 h after treatment with different concentrations of MSKE, 1 MIC ( B, F ) 2 MICs ( C, G ) and 4 MICs ( D, H ), when compared to the control, 1% DMSO, ( A, E ).
    Figure Legend Snippet: Transmission electron micrographs of S. aureus ATCC 25923 ( A –D ) and the M09 MRSA strain ( E –H ) 12 h after treatment with different concentrations of MSKE, 1 MIC ( B, F ) 2 MICs ( C, G ) and 4 MICs ( D, H ), when compared to the control, 1% DMSO, ( A, E ).

    Techniques Used: Transmission Assay

    Scanning electron micrographs of S. aureus ATCC 25923 at 12 h after treatment with ( A ) 1% DMSO (control), ( B ) MSKE at 4 MICs and ( C ) PGG at 4 MICs.
    Figure Legend Snippet: Scanning electron micrographs of S. aureus ATCC 25923 at 12 h after treatment with ( A ) 1% DMSO (control), ( B ) MSKE at 4 MICs and ( C ) PGG at 4 MICs.

    Techniques Used:

    Time–kill curves of S. aureus ATCC 25923 ( A–E ) and the M03 MRSA strain ( F–J ) after treatment with MSKE ( A and F ), PGG ( B and G ), MG ( C and H ), GA ( D and I ) and vancomycin ( E and J ). Each symbol indicates the mean ± S.D. for at least duplicate samples.
    Figure Legend Snippet: Time–kill curves of S. aureus ATCC 25923 ( A–E ) and the M03 MRSA strain ( F–J ) after treatment with MSKE ( A and F ), PGG ( B and G ), MG ( C and H ), GA ( D and I ) and vancomycin ( E and J ). Each symbol indicates the mean ± S.D. for at least duplicate samples.

    Techniques Used:

    14) Product Images from "The Antimicrobial Potential of Bacteria Isolated from Honey Samples Produced in the Apiaries Located in Pomeranian Voivodeship in Northern Poland"

    Article Title: The Antimicrobial Potential of Bacteria Isolated from Honey Samples Produced in the Apiaries Located in Pomeranian Voivodeship in Northern Poland

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph15092002

    The growth inhibition zones of  Staphylococcus aureus  ATCC 25923.
    Figure Legend Snippet: The growth inhibition zones of Staphylococcus aureus ATCC 25923.

    Techniques Used: Inhibition

    15) Product Images from "Genome-Wide Transcriptional Profiling of the Response of Staphylococcus aureus to Cryptotanshinone"

    Article Title: Genome-Wide Transcriptional Profiling of the Response of Staphylococcus aureus to Cryptotanshinone

    Journal: Journal of Biomedicine and Biotechnology

    doi: 10.1155/2009/617509

    Growth curve for S. aureus strain ATCC 25923 in the presence or absence of CT. ■, S. aureus ATCC 25923 plus 1 μ g · mL CT; Δ, S. aureus ATCC 25923 plus 2 μ g · mL CT; ∗, S. aureus ATCC 25923 plus 4 μ g · mL CT; ▲, S. aureus ATCC 25923 plus 8 μ g · mL CT; □, S. aureus ATCC 25923 plus 16 μ g · mL CT; ♦, untreated S. aureus ATCC 25923.
    Figure Legend Snippet: Growth curve for S. aureus strain ATCC 25923 in the presence or absence of CT. ■, S. aureus ATCC 25923 plus 1 μ g · mL CT; Δ, S. aureus ATCC 25923 plus 2 μ g · mL CT; ∗, S. aureus ATCC 25923 plus 4 μ g · mL CT; ▲, S. aureus ATCC 25923 plus 8 μ g · mL CT; □, S. aureus ATCC 25923 plus 16 μ g · mL CT; ♦, untreated S. aureus ATCC 25923.

    Techniques Used:

    16) Product Images from "Antibiotic-Induced Release of Lipoteichoic Acid and Peptidoglycan from Staphylococcus aureus: Quantitative Measurements and Biological Reactivities"

    Article Title: Antibiotic-Induced Release of Lipoteichoic Acid and Peptidoglycan from Staphylococcus aureus: Quantitative Measurements and Biological Reactivities

    Journal: Antimicrobial Agents and Chemotherapy

    doi:

    Release of LTA from S. aureus ATCC 25923 in the absence (■) or presence of different antibiotics at a concentration of 1× the MIC (□) or 20× the MIC ( ). Four hours after the addition of medium or antibiotics, the bacterial supernatants were collected by centrifugation and filtration of the cultures, and the release of LTA was measured by means of a specific ELISA. Antibiotics studied were the β-lactam antibiotics imipenem (imi), flucloxacillin (fluclox), and cefamandole (cefa) (A) and the protein synthesis inhibitors erythromycin (ery), clindamycin (clinda), and gentamicin (genta) (B). Note the 10-fold difference in the y axes. Data are expressed as means ± SEMs for three separate experiments, and the asterisks indicate a significant difference ( P ≤ 0.05) between the amount of LTA released during incubation with antibiotic and that found to be released by the control (contr) culture.
    Figure Legend Snippet: Release of LTA from S. aureus ATCC 25923 in the absence (■) or presence of different antibiotics at a concentration of 1× the MIC (□) or 20× the MIC ( ). Four hours after the addition of medium or antibiotics, the bacterial supernatants were collected by centrifugation and filtration of the cultures, and the release of LTA was measured by means of a specific ELISA. Antibiotics studied were the β-lactam antibiotics imipenem (imi), flucloxacillin (fluclox), and cefamandole (cefa) (A) and the protein synthesis inhibitors erythromycin (ery), clindamycin (clinda), and gentamicin (genta) (B). Note the 10-fold difference in the y axes. Data are expressed as means ± SEMs for three separate experiments, and the asterisks indicate a significant difference ( P ≤ 0.05) between the amount of LTA released during incubation with antibiotic and that found to be released by the control (contr) culture.

    Techniques Used: Concentration Assay, Centrifugation, Filtration, Enzyme-linked Immunosorbent Assay, Incubation

    17) Product Images from "Contributions of the S100A9 C-Terminal Tail to High-Affinity Mn(II) Chelation by the Host-Defense Protein Human Calprotectin"

    Article Title: Contributions of the S100A9 C-Terminal Tail to High-Affinity Mn(II) Chelation by the Host-Defense Protein Human Calprotectin

    Journal: Journal of the American Chemical Society

    doi: 10.1021/ja407147d

    Antimicrobial activity of CP-Ser and metal-binding-site mutants against E. coli ATCC 25922 (A and C) and S. aureus ATCC 25923 (B and D). Bacterial cultures were treated with CP-Ser ( 1 , ●), CP-Ser pre-incubated with 1.0 equiv Mn(II) ( 2 , ■), ΔHis 3 Asp ( 3 , ▲), ΔHis 3 Asp pre-incubated with 1.0 equiv Mn(II) ( 4 , ▼), ΔHis 4 ( 5 , ◆), (H104A) ( 6 , ▽), (E111A) ( 7 , ◣), AAA ( 8 , ○), AHA ( 9 , □), and AHA(K106H) ( 10 , △). Control experiments with untreated buffer only ( 11 ) and buffer with a 42-μM Mn(II) supplement ( 12 ) were also performed. (A and B) The OD 600 values were recorded at t = 20 h (mean ± SEM, n = 6). For clarity, the metal-binding-site mutant and Mn(II) pre-incubation assay results are shown in the left panels, and the C-terminal tail mutant assay results are shown in the right panels. (C and D) The cultures were incubated with 1.0 mg/mL protein, and OD 600 values were recorded at t = 8 and 20 h (mean ± SEM, n = 6).
    Figure Legend Snippet: Antimicrobial activity of CP-Ser and metal-binding-site mutants against E. coli ATCC 25922 (A and C) and S. aureus ATCC 25923 (B and D). Bacterial cultures were treated with CP-Ser ( 1 , ●), CP-Ser pre-incubated with 1.0 equiv Mn(II) ( 2 , ■), ΔHis 3 Asp ( 3 , ▲), ΔHis 3 Asp pre-incubated with 1.0 equiv Mn(II) ( 4 , ▼), ΔHis 4 ( 5 , ◆), (H104A) ( 6 , ▽), (E111A) ( 7 , ◣), AAA ( 8 , ○), AHA ( 9 , □), and AHA(K106H) ( 10 , △). Control experiments with untreated buffer only ( 11 ) and buffer with a 42-μM Mn(II) supplement ( 12 ) were also performed. (A and B) The OD 600 values were recorded at t = 20 h (mean ± SEM, n = 6). For clarity, the metal-binding-site mutant and Mn(II) pre-incubation assay results are shown in the left panels, and the C-terminal tail mutant assay results are shown in the right panels. (C and D) The cultures were incubated with 1.0 mg/mL protein, and OD 600 values were recorded at t = 8 and 20 h (mean ± SEM, n = 6).

    Techniques Used: Activity Assay, Binding Assay, Incubation, Mutagenesis

    18) Product Images from "Staphylococcal Persistence Due to Biofilm Formation in Synovial Fluid Containing Prophylactic Cefazolin"

    Article Title: Staphylococcal Persistence Due to Biofilm Formation in Synovial Fluid Containing Prophylactic Cefazolin

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.04579-14

    Synovial fluid containing preoperative antibiotics. (A) Representative image of a synovial fluid sample obtained during a total knee arthroplasty that contains preoperative CFZ. (B) Typical microscopic image of synovial fluid displaying high cellularity. (C) Typical microscopic image of synovial fluid displaying low cellularity. (D) Zones of inhibition (ZOI) obtained with synovial fluid containing preoperative antibiotics (2 g of CFZ). In all, at least eight different synovial fluid samples were tested on each strain in triplicate. The images shown are representative of the ZOI obtained across all samples. Shown within the panel are ZOI associated with synovial fluid from a knee aspiration that does not contain antibiotics (naive SF) ( i ), synovial fluid containing preoperative antibiotics in S. aureus ATCC 25923 ( ii ), synovial fluid containing preoperative antibiotics in an S. aureus clinical isolate ( iii ), synovial fluid containing preoperative antibiotics in S. aureus AH1710 ( iv ), synovial fluid containing preoperative antibiotics in S. epidermidis ATCC 35984 ( v ), and synovial fluid containing preoperative antibiotics in an S. aureus MRSA isolate (Thomas Jefferson University Hospital [TJU2]) ( vi ). (E) ZOIs measured digitally with ImageJ using the synovial fluid samples and strains shown in panel D. Each circle represents the average ZOI from at least three disks/strain. The red diamonds represent the ZOI obtained with 200 μg/ml CFZ/disk.
    Figure Legend Snippet: Synovial fluid containing preoperative antibiotics. (A) Representative image of a synovial fluid sample obtained during a total knee arthroplasty that contains preoperative CFZ. (B) Typical microscopic image of synovial fluid displaying high cellularity. (C) Typical microscopic image of synovial fluid displaying low cellularity. (D) Zones of inhibition (ZOI) obtained with synovial fluid containing preoperative antibiotics (2 g of CFZ). In all, at least eight different synovial fluid samples were tested on each strain in triplicate. The images shown are representative of the ZOI obtained across all samples. Shown within the panel are ZOI associated with synovial fluid from a knee aspiration that does not contain antibiotics (naive SF) ( i ), synovial fluid containing preoperative antibiotics in S. aureus ATCC 25923 ( ii ), synovial fluid containing preoperative antibiotics in an S. aureus clinical isolate ( iii ), synovial fluid containing preoperative antibiotics in S. aureus AH1710 ( iv ), synovial fluid containing preoperative antibiotics in S. epidermidis ATCC 35984 ( v ), and synovial fluid containing preoperative antibiotics in an S. aureus MRSA isolate (Thomas Jefferson University Hospital [TJU2]) ( vi ). (E) ZOIs measured digitally with ImageJ using the synovial fluid samples and strains shown in panel D. Each circle represents the average ZOI from at least three disks/strain. The red diamonds represent the ZOI obtained with 200 μg/ml CFZ/disk.

    Techniques Used: Inhibition

    Bacterial growth and clumping in synovial fluid. (A) i , 5-h culture of S. aureus ). Note the S. aureus aggregate that is boxed in red. ii , magnification at 10× of the aggregated S. aureus ATCC 25923 that was shown within the red box. iii , aggregated bacteria after incubation in SF6.1, SF6.2, or SF 6.3, each containing 2 g of CFZ from preoperative prophylaxis, visualized by ethidium bromide staining, and photographed. Scale bar = 1.5 mm. iv , scanning electron micrograph of an aggregate formed in naive synovial fluid. Scale bar = 50 μm. v , three-dimensional reconstruction of confocal laser scanning micrographs of an S. aureus ATCC 25923 aggregate formed in synovial fluid containing 2 g of preoperative CFZ. Similar images were obtained with other synovial fluid samples containing preoperative CFZ. (B) WGA staining for PIA/PNAG in S. aureus ATCC 25923 shown incubated in three synovial fluid samples ( i ) (left to right: SF3.1, SF3.2, and SF3.3) or three separate cultures of TSB without antibiotics ( ii ).
    Figure Legend Snippet: Bacterial growth and clumping in synovial fluid. (A) i , 5-h culture of S. aureus ). Note the S. aureus aggregate that is boxed in red. ii , magnification at 10× of the aggregated S. aureus ATCC 25923 that was shown within the red box. iii , aggregated bacteria after incubation in SF6.1, SF6.2, or SF 6.3, each containing 2 g of CFZ from preoperative prophylaxis, visualized by ethidium bromide staining, and photographed. Scale bar = 1.5 mm. iv , scanning electron micrograph of an aggregate formed in naive synovial fluid. Scale bar = 50 μm. v , three-dimensional reconstruction of confocal laser scanning micrographs of an S. aureus ATCC 25923 aggregate formed in synovial fluid containing 2 g of preoperative CFZ. Similar images were obtained with other synovial fluid samples containing preoperative CFZ. (B) WGA staining for PIA/PNAG in S. aureus ATCC 25923 shown incubated in three synovial fluid samples ( i ) (left to right: SF3.1, SF3.2, and SF3.3) or three separate cultures of TSB without antibiotics ( ii ).

    Techniques Used: Incubation, Staining, Whole Genome Amplification

    19) Product Images from "Discrepancy between Uptake and Intracellular Activity of Moxifloxacin in a Staphylococcus aureus-Human THP-1 Monocytic Cell Model"

    Article Title: Discrepancy between Uptake and Intracellular Activity of Moxifloxacin in a Staphylococcus aureus-Human THP-1 Monocytic Cell Model

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.46.2.288-293.2002

    Influence of moxifloxacin on the viability of S. aureus ATCC 25923 using inocula of 10 5 (A) and 10 7 CFU/ml (B). The antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in two determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter; ×, 0.1 mg/liter ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.
    Figure Legend Snippet: Influence of moxifloxacin on the viability of S. aureus ATCC 25923 using inocula of 10 5 (A) and 10 7 CFU/ml (B). The antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in two determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter; ×, 0.1 mg/liter ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.

    Techniques Used:

    Effect of moxifloxacin on the viability of S. aureus ATCC 25923 ingested by THP-1 cells. Antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in three determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter, ×, 0.1 mg/liter; ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.
    Figure Legend Snippet: Effect of moxifloxacin on the viability of S. aureus ATCC 25923 ingested by THP-1 cells. Antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in three determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter, ×, 0.1 mg/liter; ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.

    Techniques Used:

    20) Product Images from "Prevention of Staphylococcus aureus biofilm formation by antibiotics in 96-Microtiter Well Plates and Drip Flow Reactors: critical factors influencing outcomes"

    Article Title: Prevention of Staphylococcus aureus biofilm formation by antibiotics in 96-Microtiter Well Plates and Drip Flow Reactors: critical factors influencing outcomes

    Journal: Scientific Reports

    doi: 10.1038/srep43854

    Confocal laser scanning microscopy images of S. aureus ATCC 25923 biofilms grown in MWP ( A – C ) and DFR ( D – F ). ( A – C ) are representatives taken from different locations of one well in a MWP and D-F from different locations within one coupon in DFR. Biofilms are stained with LIVE/DEAD BacLight kit. Scale bars correspond to 30 μM.
    Figure Legend Snippet: Confocal laser scanning microscopy images of S. aureus ATCC 25923 biofilms grown in MWP ( A – C ) and DFR ( D – F ). ( A – C ) are representatives taken from different locations of one well in a MWP and D-F from different locations within one coupon in DFR. Biofilms are stained with LIVE/DEAD BacLight kit. Scale bars correspond to 30 μM.

    Techniques Used: Confocal Laser Scanning Microscopy, Staining

    21) Product Images from "“Smart” Antimicrobial Nanocomplexes with Potential to Decrease Surgical Site Infections (SSI)"

    Article Title: “Smart” Antimicrobial Nanocomplexes with Potential to Decrease Surgical Site Infections (SSI)

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics12040361

    Antimicrobial agar plate methods on AgNP compounds with positive controls (antibiotic). From left to right: ( a ) Agar well diffusion of TCA-AgNP against S. aureus ATCC 25923 ; ( b ) TCA-AgNP-PI against P. aeruginosa WDCM 00026 . From ( c – f ) disc diffusion methods: ( c ) TCA-AgNP against S. aureus ATCC 25932 ; ( d ) TCA-AgNP against S. aureus ATCC 25932 ; ( e ) TCA-AgNP-PI against E. coli WDCM 00013 ; ( f ) Cinn-AgNP-PI against E. coli WDCM 00013 .
    Figure Legend Snippet: Antimicrobial agar plate methods on AgNP compounds with positive controls (antibiotic). From left to right: ( a ) Agar well diffusion of TCA-AgNP against S. aureus ATCC 25923 ; ( b ) TCA-AgNP-PI against P. aeruginosa WDCM 00026 . From ( c – f ) disc diffusion methods: ( c ) TCA-AgNP against S. aureus ATCC 25932 ; ( d ) TCA-AgNP against S. aureus ATCC 25932 ; ( e ) TCA-AgNP-PI against E. coli WDCM 00013 ; ( f ) Cinn-AgNP-PI against E. coli WDCM 00013 .

    Techniques Used: Diffusion-based Assay

    Antimicrobial agar plate methods of TCA-AgNP-PI dip-coated PGA with positive controls (antibiotics). From left to right: against ( a ) S. aureus ATCC 25923 ; ( b ) E. coli WDCM 00013 ; ( c ) clinical sample Bacillus subtilis ; ( d ) P. mirabilis ATCC 29906 ; ( e ) S. pyogenes ATCC 19615 .
    Figure Legend Snippet: Antimicrobial agar plate methods of TCA-AgNP-PI dip-coated PGA with positive controls (antibiotics). From left to right: against ( a ) S. aureus ATCC 25923 ; ( b ) E. coli WDCM 00013 ; ( c ) clinical sample Bacillus subtilis ; ( d ) P. mirabilis ATCC 29906 ; ( e ) S. pyogenes ATCC 19615 .

    Techniques Used:

    22) Product Images from "Ability of Laboratories To Detect Emerging Antimicrobial Resistance: Proficiency Testing and Quality Control Results from the World Health Organization's External Quality Assurance System for Antimicrobial Susceptibility Testing"

    Article Title: Ability of Laboratories To Detect Emerging Antimicrobial Resistance: Proficiency Testing and Quality Control Results from the World Health Organization's External Quality Assurance System for Antimicrobial Susceptibility Testing

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.39.1.241-250.2001

    Histogram showing quality control results for disk diffusion testing of S. aureus ATCC 25923 against vancomycin. The NCCLS quality control range against which results were compared was 17 to 21 mm. Data are all results received as of 25 August 1999 and tested by NCCLS methods.
    Figure Legend Snippet: Histogram showing quality control results for disk diffusion testing of S. aureus ATCC 25923 against vancomycin. The NCCLS quality control range against which results were compared was 17 to 21 mm. Data are all results received as of 25 August 1999 and tested by NCCLS methods.

    Techniques Used: Diffusion-based Assay

    Histogram showing quality control results for disk diffusion testing of S. aureus ATCC 25923 against oxacillin. The NCCLS quality control range against which results were compared was 18 to 24 mm. Data are all results received as of 30 August 1999 and tested by NCCLS methods.
    Figure Legend Snippet: Histogram showing quality control results for disk diffusion testing of S. aureus ATCC 25923 against oxacillin. The NCCLS quality control range against which results were compared was 18 to 24 mm. Data are all results received as of 30 August 1999 and tested by NCCLS methods.

    Techniques Used: Diffusion-based Assay

    23) Product Images from "Antibiotic screening of urine culture as a tool for interal quality audit"

    Article Title: Antibiotic screening of urine culture as a tool for interal quality audit

    Journal: The Australasian Medical Journal

    doi: 10.4066/AMJ.2014.1956

    Modified UABA using E.coli ATCC 25922 and Staph.aureus ATCC 25923
    Figure Legend Snippet: Modified UABA using E.coli ATCC 25922 and Staph.aureus ATCC 25923

    Techniques Used: Modification

    24) Product Images from "Antibiotic screening of urine culture as a tool for interal quality audit"

    Article Title: Antibiotic screening of urine culture as a tool for interal quality audit

    Journal: The Australasian Medical Journal

    doi: 10.4066/AMJ.2014.1956

    Modified UABA using E.coli ATCC 25922 and Staph.aureus ATCC 25923
    Figure Legend Snippet: Modified UABA using E.coli ATCC 25922 and Staph.aureus ATCC 25923

    Techniques Used: Modification

    25) Product Images from "Paromomycin production from Streptomyces rimosus NRRL 2455: statistical optimization and new synergistic antibiotic combinations against multidrug resistant pathogens"

    Article Title: Paromomycin production from Streptomyces rimosus NRRL 2455: statistical optimization and new synergistic antibiotic combinations against multidrug resistant pathogens

    Journal: BMC Microbiology

    doi: 10.1186/s12866-019-1390-1

    Calibration Curve of standard paromomycin antibacterial activity against Staphylococcus aureus ATCC 25923
    Figure Legend Snippet: Calibration Curve of standard paromomycin antibacterial activity against Staphylococcus aureus ATCC 25923

    Techniques Used: Activity Assay

    26) Product Images from "Candida albicans and Staphylococcus aureus Form Polymicrobial Biofilms: Effects on Antimicrobial Resistance "

    Article Title: Candida albicans and Staphylococcus aureus Form Polymicrobial Biofilms: Effects on Antimicrobial Resistance

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00657-09

    Effect of planktonic polymicrobial growth on antimicrobial drug resistance. C. albicans SC5314 (10 3 CFU/ml) alone, S. aureus ATCC 25923 (10 3 CFU/ml) alone, or C. albicans (10 3 CFU/ml) and S. aureus (10 3 CFU/ml) together were added to 96-well, deep-well polypropylene plates in 50% BS. Amp B alone, vancomycin alone, or Amp B and vancomycin combined were added to the wells. The plates were incubated at 35°C in an orbital shaker at 175 rpm for 18 h, and fungal and bacterial viability was monitored by the CFU assay. Experiments were performed in duplicate, and data represent averages of two independent experiments. CA, C. albicans ; SA, S. aureus ; Mono, monomicrobial biofilm; Poly, polymicrobial biofilm.
    Figure Legend Snippet: Effect of planktonic polymicrobial growth on antimicrobial drug resistance. C. albicans SC5314 (10 3 CFU/ml) alone, S. aureus ATCC 25923 (10 3 CFU/ml) alone, or C. albicans (10 3 CFU/ml) and S. aureus (10 3 CFU/ml) together were added to 96-well, deep-well polypropylene plates in 50% BS. Amp B alone, vancomycin alone, or Amp B and vancomycin combined were added to the wells. The plates were incubated at 35°C in an orbital shaker at 175 rpm for 18 h, and fungal and bacterial viability was monitored by the CFU assay. Experiments were performed in duplicate, and data represent averages of two independent experiments. CA, C. albicans ; SA, S. aureus ; Mono, monomicrobial biofilm; Poly, polymicrobial biofilm.

    Techniques Used: Incubation, Colony-forming Unit Assay

    27) Product Images from "Acquisition of Complement Inhibitor Serine Protease Factor I and Its Cofactors C4b-Binding Protein and Factor H by Prevotella intermedia"

    Article Title: Acquisition of Complement Inhibitor Serine Protease Factor I and Its Cofactors C4b-Binding Protein and Factor H by Prevotella intermedia

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0034852

    P. intermedia  isolates bind purified FI cofactors C4BP and FH. The indicated  P. intermedia  strains, as well as A)  M. catarrhalis  RH 4,  P. gingivalis  W50 and W83 (C4BP binding positive controls),  M. catarrhalis  Δ uspA 1/2, and  E. coli  DH5α (C4BP binding negative controls) or B)  S. pyogenes  CCUG 25571 (FH binding positive control),  S. aureus  ATCC 25923, and  E. coli  DH5α (FH binding negative controls) were mixed with (A) 500 kcpm  125 I-C4BP or (B)  125 I-FH and incubated for 1 h at RT. Proteins bound to bacteria were detected as described in   Fig. 1 . Samples containing  125 I-labeled proteins that were incubated with buffer alone served as negative controls. Bars represent averages of three independent experiments performed in duplicates and SD are indicated as error bars. One-way ANOVA and Tukey's post-hoc test was used for statistical analysis as compared to negative controls (*** p
    Figure Legend Snippet: P. intermedia isolates bind purified FI cofactors C4BP and FH. The indicated P. intermedia strains, as well as A) M. catarrhalis RH 4, P. gingivalis W50 and W83 (C4BP binding positive controls), M. catarrhalis Δ uspA 1/2, and E. coli DH5α (C4BP binding negative controls) or B) S. pyogenes CCUG 25571 (FH binding positive control), S. aureus ATCC 25923, and E. coli DH5α (FH binding negative controls) were mixed with (A) 500 kcpm 125 I-C4BP or (B) 125 I-FH and incubated for 1 h at RT. Proteins bound to bacteria were detected as described in Fig. 1 . Samples containing 125 I-labeled proteins that were incubated with buffer alone served as negative controls. Bars represent averages of three independent experiments performed in duplicates and SD are indicated as error bars. One-way ANOVA and Tukey's post-hoc test was used for statistical analysis as compared to negative controls (*** p

    Techniques Used: Purification, Binding Assay, Positive Control, Incubation, Labeling

    P. intermedia strains bind purified 125 I-FI and FI from HI-NHS. A) P. intermedia ATCC 25611, OMZ 248, OMZ 324, MH6, S. aureus strains ATCC 25923 and Newman (FI binding positive control) as well as E. coli DH5α (FI binding negative control) were incubated with 125 I-FI (250 kcpm). Bound and free FI were separated by centrifugation through sucrose, the radioactivity associated with pellets and supernatants was measured in a gamma counter and the percentage of radioactivity bound to the pellet was calculated. Samples containing 125 I-labeled FI that was incubated with buffer alone served as a negative control. Averages of three independent experiments performed in duplicates are shown; error bars show SD. One-way ANOVA followed by Tukey's post-hoc test was used for statistical evaluation of the data as compared to the negative control (*** p
    Figure Legend Snippet: P. intermedia strains bind purified 125 I-FI and FI from HI-NHS. A) P. intermedia ATCC 25611, OMZ 248, OMZ 324, MH6, S. aureus strains ATCC 25923 and Newman (FI binding positive control) as well as E. coli DH5α (FI binding negative control) were incubated with 125 I-FI (250 kcpm). Bound and free FI were separated by centrifugation through sucrose, the radioactivity associated with pellets and supernatants was measured in a gamma counter and the percentage of radioactivity bound to the pellet was calculated. Samples containing 125 I-labeled FI that was incubated with buffer alone served as a negative control. Averages of three independent experiments performed in duplicates are shown; error bars show SD. One-way ANOVA followed by Tukey's post-hoc test was used for statistical evaluation of the data as compared to the negative control (*** p

    Techniques Used: Purification, Binding Assay, Positive Control, Negative Control, Incubation, Centrifugation, Radioactivity, Labeling

    28) Product Images from "Biogenic selenium and tellurium nanoparticles synthesized by environmental microbial isolates efficaciously inhibit bacterial planktonic cultures and biofilms"

    Article Title: Biogenic selenium and tellurium nanoparticles synthesized by environmental microbial isolates efficaciously inhibit bacterial planktonic cultures and biofilms

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2015.00584

    EC 50 of SeNPs and TeNPs for planktonic and biofilm cultures of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) after 4 h of exposure and 24 h of growth + 24 h of exposure .
    Figure Legend Snippet: EC 50 of SeNPs and TeNPs for planktonic and biofilm cultures of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) after 4 h of exposure and 24 h of growth + 24 h of exposure .

    Techniques Used:

    MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of SeNPs ( n = 3) .
    Figure Legend Snippet: MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of SeNPs ( n = 3) .

    Techniques Used:

    MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of TeNPs ( n = 3) .
    Figure Legend Snippet: MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of TeNPs ( n = 3) .

    Techniques Used:

    29) Product Images from "Biogenic selenium and tellurium nanoparticles synthesized by environmental microbial isolates efficaciously inhibit bacterial planktonic cultures and biofilms"

    Article Title: Biogenic selenium and tellurium nanoparticles synthesized by environmental microbial isolates efficaciously inhibit bacterial planktonic cultures and biofilms

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2015.00584

    EC 50 of SeNPs and TeNPs for planktonic and biofilm cultures of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) after 4 h of exposure and 24 h of growth + 24 h of exposure .
    Figure Legend Snippet: EC 50 of SeNPs and TeNPs for planktonic and biofilm cultures of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) after 4 h of exposure and 24 h of growth + 24 h of exposure .

    Techniques Used:

    MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of SeNPs ( n = 3) .
    Figure Legend Snippet: MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of SeNPs ( n = 3) .

    Techniques Used:

    MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of TeNPs ( n = 3) .
    Figure Legend Snippet: MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of TeNPs ( n = 3) .

    Techniques Used:

    30) Product Images from "Daptomycin Antibiotic Lock Therapy in a Rat Model of Staphylococcal Central Venous Catheter Biofilm Infections ▿"

    Article Title: Daptomycin Antibiotic Lock Therapy in a Rat Model of Staphylococcal Central Venous Catheter Biofilm Infections ▿

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.00147-11

    ALT and parenteral daptomycin treatment, separately and together, of methicillin-susceptible Staphylococcus aureus (MSSA) central venous catheter infection. (A) Daily central venous catheter blood draws were used to monitor S. aureus ATCC 25923 biofilms
    Figure Legend Snippet: ALT and parenteral daptomycin treatment, separately and together, of methicillin-susceptible Staphylococcus aureus (MSSA) central venous catheter infection. (A) Daily central venous catheter blood draws were used to monitor S. aureus ATCC 25923 biofilms

    Techniques Used: Infection

    31) Product Images from "Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells"

    Article Title: Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells

    Journal: Microorganisms

    doi: 10.3390/microorganisms8030345

    Biofilm biomass produced by S. aureus ATCC 25923 in comparison with five clinical strains resulting after 18 h incubation ( a ) and 42 h incubation ( b ). Statistical differences are indicated in comparison to the collection strain, ** p
    Figure Legend Snippet: Biofilm biomass produced by S. aureus ATCC 25923 in comparison with five clinical strains resulting after 18 h incubation ( a ) and 42 h incubation ( b ). Statistical differences are indicated in comparison to the collection strain, ** p

    Techniques Used: Produced, Incubation

    Effect of the two DHA derivatives ( DHA1 and DHA2 ), the flavonoid derivative ( FLA1 ) and two control antibiotics ( RIF and PEN ) on competitive colonization on titanium coupons pre-incubated for 24 h with SaOS-2 cells. ( a ) Results corresponding to the viability of SaOS-2 cells when cultured on titanium coupons alone (SaOS-2, grey bars), or co-cultured with S. aureus ATCC 25923 (SaOS-2 + S. aureus 25923, blue bars), or P2 clinical S. aureus strain (SaOS-2 + S. aureus P2, red bars); ( b , c ) Results corresponding to the effects on attached viable S. aureus measured when the ATCC 25923 ( b ) or P2 clinical S. aureus strain ( c ) was used. Percentage of viability of SaOS-2 cells was calculated with respect to untreated controls after 24-h incubation on titanium coupons, using glow luminescence signal resulting from ATP production by viable SaOS-2 cells. Viable counts (log of CFU/mL) of S. aureus ATCC 25923 or the clinical strain P2 were also measured after 24-h incubation on titanium coupons when co-cultured with SaOS-2 cells. “*” represents differences with the corresponding monoculture control and “#” represents differences with the corresponding co-culture controls (* p
    Figure Legend Snippet: Effect of the two DHA derivatives ( DHA1 and DHA2 ), the flavonoid derivative ( FLA1 ) and two control antibiotics ( RIF and PEN ) on competitive colonization on titanium coupons pre-incubated for 24 h with SaOS-2 cells. ( a ) Results corresponding to the viability of SaOS-2 cells when cultured on titanium coupons alone (SaOS-2, grey bars), or co-cultured with S. aureus ATCC 25923 (SaOS-2 + S. aureus 25923, blue bars), or P2 clinical S. aureus strain (SaOS-2 + S. aureus P2, red bars); ( b , c ) Results corresponding to the effects on attached viable S. aureus measured when the ATCC 25923 ( b ) or P2 clinical S. aureus strain ( c ) was used. Percentage of viability of SaOS-2 cells was calculated with respect to untreated controls after 24-h incubation on titanium coupons, using glow luminescence signal resulting from ATP production by viable SaOS-2 cells. Viable counts (log of CFU/mL) of S. aureus ATCC 25923 or the clinical strain P2 were also measured after 24-h incubation on titanium coupons when co-cultured with SaOS-2 cells. “*” represents differences with the corresponding monoculture control and “#” represents differences with the corresponding co-culture controls (* p

    Techniques Used: Incubation, Cell Culture, Co-Culture Assay

    Effect of the DHA derivative DHA1 on the competitive colonization assay performed in titanium coupons pre-conditioned with media supplemented with FBS. ( a , b ) Results corresponding to the viability of SaOS-2 cells when ATCC 25923 ( a , blue bars) or P2 clinical S. aureus strain ( b , red bars) were used. ( c , d ) Results corresponding to the effects of attached viable S. aureus measured when ATCC 25923 ( c , blue bars) or P2 clinical strain ( d , red bars) were used. The percentage of viability of SaOS-2 cells was calculated with respect to untreated controls after 24-h incubation on titanium coupons, using glow luminescence signal resulting from ATP production by viable SaOS-2 cells. Viable counts (log of CFU/mL) of S. aureus 25923 and the clinical strain P2, respectively, were also measured after 24-h incubation on titanium coupons when co-cultured with SaOS-2 cells. “*” represents differences with the control in mono-culture exposed to the non-preconditioned titanium coupon (SaOS-2 non- preconditioned with FBS/ S. aureus 25923 non-preconditioned/ S. aureus P2 non-preconditioned). “#” represents differences with the control of the subgroups (* p
    Figure Legend Snippet: Effect of the DHA derivative DHA1 on the competitive colonization assay performed in titanium coupons pre-conditioned with media supplemented with FBS. ( a , b ) Results corresponding to the viability of SaOS-2 cells when ATCC 25923 ( a , blue bars) or P2 clinical S. aureus strain ( b , red bars) were used. ( c , d ) Results corresponding to the effects of attached viable S. aureus measured when ATCC 25923 ( c , blue bars) or P2 clinical strain ( d , red bars) were used. The percentage of viability of SaOS-2 cells was calculated with respect to untreated controls after 24-h incubation on titanium coupons, using glow luminescence signal resulting from ATP production by viable SaOS-2 cells. Viable counts (log of CFU/mL) of S. aureus 25923 and the clinical strain P2, respectively, were also measured after 24-h incubation on titanium coupons when co-cultured with SaOS-2 cells. “*” represents differences with the control in mono-culture exposed to the non-preconditioned titanium coupon (SaOS-2 non- preconditioned with FBS/ S. aureus 25923 non-preconditioned/ S. aureus P2 non-preconditioned). “#” represents differences with the control of the subgroups (* p

    Techniques Used: Incubation, Cell Culture

    Representative fluorescence microscope images of titanium coupons treated under different conditions in a competitive colonization model with cellular pre-coating. Upper row of the images ( Figure 7 a–c) correspond to the controls: ( a ) titanium covered by 10 5 human cells/mL (cell control); ( b ) titanium covered by 10 4 CFU/mL of S. aureus ATCC 25923 (bacterial control), and ( c ) 10 4 CFU/mL of S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL (co-culture control). Middle row of images ( Figure 7 d–f) correspond to titanium coupons with the two DHA derivatives ( d ) DHA1 and ( e ) DHA2 , and the flavonoid derivative ( f ) FLA1, all added at a concentration of 50 µM and co-cultured with S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL. The bottom row of images ( Figure 7 g,h) are representative images of titanium coupons coated with the two control antibiotics ( g ) RIF and ( h ) PEN , all added at a concentration of 50 µM and co-cultured with 10 4 CFU/mL of S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL. The samples were stained with acridine orange (BD Diagnostics, Sparks, MD, USA).
    Figure Legend Snippet: Representative fluorescence microscope images of titanium coupons treated under different conditions in a competitive colonization model with cellular pre-coating. Upper row of the images ( Figure 7 a–c) correspond to the controls: ( a ) titanium covered by 10 5 human cells/mL (cell control); ( b ) titanium covered by 10 4 CFU/mL of S. aureus ATCC 25923 (bacterial control), and ( c ) 10 4 CFU/mL of S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL (co-culture control). Middle row of images ( Figure 7 d–f) correspond to titanium coupons with the two DHA derivatives ( d ) DHA1 and ( e ) DHA2 , and the flavonoid derivative ( f ) FLA1, all added at a concentration of 50 µM and co-cultured with S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL. The bottom row of images ( Figure 7 g,h) are representative images of titanium coupons coated with the two control antibiotics ( g ) RIF and ( h ) PEN , all added at a concentration of 50 µM and co-cultured with 10 4 CFU/mL of S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL. The samples were stained with acridine orange (BD Diagnostics, Sparks, MD, USA).

    Techniques Used: Fluorescence, Microscopy, Co-Culture Assay, Concentration Assay, Cell Culture, Staining

    Effect of the two DHA derivatives ( DHA1 and DHA2 ), the flavonoid-derivative ( FLA1 ), and two control antibiotics ( RIF and PEN ) on the competitive colonization assay performed in titanium coupons. ( a ) Results corresponding to the viability of SaOS-2 cells when cultured on titanium coupons alone (SaOS-2, grey bars), or co-cultured with S. aureus ATCC 25923 (SaOS-2 + S. aureus 25923, blue bars), or P2 clinical S. aureus strain (SaOS-2 + S. aureus P2, red bars); ( b , c ) Results corresponding to the effects on attached viable S. aureus measured when the ATCC 25923 ( b ) or P2 clinical S. aureus strain ( c ) was used. Percentage of viability of SaOS-2 cells was calculated with respect to untreated controls after 24-h incubation on titanium coupons, using glow luminescence signal resulting from ATP production by viable SaOS-2 cells. Viable counts (log of CFU/mL) of S. aureus ATCC 25923 or the clinical strain P2 were also measured after 24-h incubation on titanium coupons when co-cultured with SaOS-2 cells. “*” represents differences with the corresponding monoculture control and “#” represents differences with the corresponding co-culture controls (* p
    Figure Legend Snippet: Effect of the two DHA derivatives ( DHA1 and DHA2 ), the flavonoid-derivative ( FLA1 ), and two control antibiotics ( RIF and PEN ) on the competitive colonization assay performed in titanium coupons. ( a ) Results corresponding to the viability of SaOS-2 cells when cultured on titanium coupons alone (SaOS-2, grey bars), or co-cultured with S. aureus ATCC 25923 (SaOS-2 + S. aureus 25923, blue bars), or P2 clinical S. aureus strain (SaOS-2 + S. aureus P2, red bars); ( b , c ) Results corresponding to the effects on attached viable S. aureus measured when the ATCC 25923 ( b ) or P2 clinical S. aureus strain ( c ) was used. Percentage of viability of SaOS-2 cells was calculated with respect to untreated controls after 24-h incubation on titanium coupons, using glow luminescence signal resulting from ATP production by viable SaOS-2 cells. Viable counts (log of CFU/mL) of S. aureus ATCC 25923 or the clinical strain P2 were also measured after 24-h incubation on titanium coupons when co-cultured with SaOS-2 cells. “*” represents differences with the corresponding monoculture control and “#” represents differences with the corresponding co-culture controls (* p

    Techniques Used: Cell Culture, Incubation, Co-Culture Assay

    Representative fluorescence microscope images of titanium coupons treated under different conditions in the competitive colonization model. Upper row of the images ( Figure 5 a–c) correspond to the controls: ( a ) titanium covered by 10 5 human cells/mL (cell control); ( b ) titanium covered by 10 4 CFU/mL of S. aureus ATCC 25923 (bacterial control), and ( c ) 10 4 CFU/mL of S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL (co-culture control). Middle row of images ( Figure 5 d–f) correspond to titanium coupons with the two DHA derivatives ( d ) DHA1 and ( e ) DHA2 , and the flavonoid-derivative ( f ) FLA1 , all added at a concentration of 50 µM and co-cultured with S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL. The bottom row of images ( Figure 5 g,h) are representative images of titanium coupons with the two control antibiotics ( g ) RIF and ( h ) PEN , all added at a concentration of 50 µM and co-cultured with 10 4 CFU/mL of S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL. The samples were stained with acridine orange (BD Diagnostics, Sparks, MD, USA).
    Figure Legend Snippet: Representative fluorescence microscope images of titanium coupons treated under different conditions in the competitive colonization model. Upper row of the images ( Figure 5 a–c) correspond to the controls: ( a ) titanium covered by 10 5 human cells/mL (cell control); ( b ) titanium covered by 10 4 CFU/mL of S. aureus ATCC 25923 (bacterial control), and ( c ) 10 4 CFU/mL of S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL (co-culture control). Middle row of images ( Figure 5 d–f) correspond to titanium coupons with the two DHA derivatives ( d ) DHA1 and ( e ) DHA2 , and the flavonoid-derivative ( f ) FLA1 , all added at a concentration of 50 µM and co-cultured with S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL. The bottom row of images ( Figure 5 g,h) are representative images of titanium coupons with the two control antibiotics ( g ) RIF and ( h ) PEN , all added at a concentration of 50 µM and co-cultured with 10 4 CFU/mL of S. aureus ATCC 25923 and 10 5 human SaOS-2 cells/mL. The samples were stained with acridine orange (BD Diagnostics, Sparks, MD, USA).

    Techniques Used: Fluorescence, Microscopy, Co-Culture Assay, Concentration Assay, Cell Culture, Staining

    32) Product Images from "Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria"

    Article Title: Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-18590-6

    Antibacterial activity of AgNPs against different bacteria ( E .  coli  ATCC 25922,  S. aureus  ATCC 25923,  B. subtilis ,  P. aeruginosa ,  K. pneumoniae  AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.
    Figure Legend Snippet: Antibacterial activity of AgNPs against different bacteria ( E . coli ATCC 25922, S. aureus ATCC 25923, B. subtilis , P. aeruginosa , K. pneumoniae AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.

    Techniques Used: Activity Assay

    33) Product Images from "Facile Synthesis of Antimicrobial Aloe Vera-“Smart” Triiodide-PVP Biomaterials"

    Article Title: Facile Synthesis of Antimicrobial Aloe Vera-“Smart” Triiodide-PVP Biomaterials

    Journal: Biomimetics

    doi: 10.3390/biomimetics5030045

    Antimicrobial disc dilution assay of biocomplexes with positive controls (antibiotic). From left to right: AV-PVP-I 2 against ( a ) S. aureus ATCC 25923 ; ( b ) P. aeruginosa WDCM 00026; ( c ) B. subtilis WDCM 00003 . From ( d , e ): AV-PVP-I 2 -NaI against ( d ) S. aureus ATCC 25932 ; ( e ) B. subtilis WDCM 00003 ; ( f ) AV-PVP-I 2 , AV-PVP-NaI and AV-PVP-NaI against S. pyogenes ATCC 19615 . From ( g – i ): Susceptibility of C. albicans WDCM 00054 towards ( g ) AV-PVP-I 2 (25 µg/mL); ( h ) AV-PVP-I 2 -NaI (50 µg/mL); ( i ) AV-PVP-I 2 -NaI (D = 6 µg/mL, E = 3 µg/mL, F = 1.5 µg/mL).
    Figure Legend Snippet: Antimicrobial disc dilution assay of biocomplexes with positive controls (antibiotic). From left to right: AV-PVP-I 2 against ( a ) S. aureus ATCC 25923 ; ( b ) P. aeruginosa WDCM 00026; ( c ) B. subtilis WDCM 00003 . From ( d , e ): AV-PVP-I 2 -NaI against ( d ) S. aureus ATCC 25932 ; ( e ) B. subtilis WDCM 00003 ; ( f ) AV-PVP-I 2 , AV-PVP-NaI and AV-PVP-NaI against S. pyogenes ATCC 19615 . From ( g – i ): Susceptibility of C. albicans WDCM 00054 towards ( g ) AV-PVP-I 2 (25 µg/mL); ( h ) AV-PVP-I 2 -NaI (50 µg/mL); ( i ) AV-PVP-I 2 -NaI (D = 6 µg/mL, E = 3 µg/mL, F = 1.5 µg/mL).

    Techniques Used: Dilution Assay

    34) Product Images from "Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation"

    Article Title: Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation

    Journal: Microorganisms

    doi: 10.3390/microorganisms8111757

    Viable counts of adhered S. aureus ATCC 25923 ( a ) and S. aureus P2 ( b ) on titanium coupons exposed to different antimicrobial compounds (tested at 50 µM) after 24 h of incubation. The gray bars show the attached viable bacteria when exposed just to the antimicrobial compounds, while the green bars show the results when S. aureus strains were cocultured with HL-60 cells. “*” indicates statistical differences with the control in monoculture while “#” represents statistical differences with the control in cocultured controls with Welch’s unpaired t -test ( * p
    Figure Legend Snippet: Viable counts of adhered S. aureus ATCC 25923 ( a ) and S. aureus P2 ( b ) on titanium coupons exposed to different antimicrobial compounds (tested at 50 µM) after 24 h of incubation. The gray bars show the attached viable bacteria when exposed just to the antimicrobial compounds, while the green bars show the results when S. aureus strains were cocultured with HL-60 cells. “*” indicates statistical differences with the control in monoculture while “#” represents statistical differences with the control in cocultured controls with Welch’s unpaired t -test ( * p

    Techniques Used: Incubation

    Viable counts of 24-hour-old biofilms formed by different concentrations of S. aureus ATCC 25923 cocultured with HL-60 cells on 96-well microplates. Black columns represent the bacterial control (viable attached cells in the absence of HL-60 cells). Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA). Values are means and SD of three independent experiments ( *** p
    Figure Legend Snippet: Viable counts of 24-hour-old biofilms formed by different concentrations of S. aureus ATCC 25923 cocultured with HL-60 cells on 96-well microplates. Black columns represent the bacterial control (viable attached cells in the absence of HL-60 cells). Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA). Values are means and SD of three independent experiments ( *** p

    Techniques Used:

    Viable counts of adhered S. aureus ATCC 25923 on LDPE tubes exposed to different antimicrobial compounds (tested at 50 µM) after 24 h of incubation. The gray bars show the attached viable bacteria when exposed just to the antimicrobial compounds, while the green bars show the results when S. aureus strains were cocultured with HL-60 cells. “*” indicates statistical differences with the control in monoculture, while “#” represents statistical differences with the control in cocultured controls with Welch’s unpaired t -test ( ** p
    Figure Legend Snippet: Viable counts of adhered S. aureus ATCC 25923 on LDPE tubes exposed to different antimicrobial compounds (tested at 50 µM) after 24 h of incubation. The gray bars show the attached viable bacteria when exposed just to the antimicrobial compounds, while the green bars show the results when S. aureus strains were cocultured with HL-60 cells. “*” indicates statistical differences with the control in monoculture, while “#” represents statistical differences with the control in cocultured controls with Welch’s unpaired t -test ( ** p

    Techniques Used: Incubation

    Representative images acquired by SEM. From left to right the same section of the titanium coupon is shown with different magnification, 500, 1000 and 3000×. Upper row of images, bacterial control, i.e., coupons incubated with S. aureus ATCC 25923 only. Second row, titanium coupons incubated with S. aureus ATCC 25923 and treated with DHA1 . Third row, cocultured control, titanium coupons incubated with S. aureus ATCC 25923 and HL-60 cells simultaneously. Fourth row, titanium coupons cocultured with S. aureus ATCC 25923 and HL-60 and treated with DHA1 .
    Figure Legend Snippet: Representative images acquired by SEM. From left to right the same section of the titanium coupon is shown with different magnification, 500, 1000 and 3000×. Upper row of images, bacterial control, i.e., coupons incubated with S. aureus ATCC 25923 only. Second row, titanium coupons incubated with S. aureus ATCC 25923 and treated with DHA1 . Third row, cocultured control, titanium coupons incubated with S. aureus ATCC 25923 and HL-60 cells simultaneously. Fourth row, titanium coupons cocultured with S. aureus ATCC 25923 and HL-60 and treated with DHA1 .

    Techniques Used: Incubation

    Viable counts of adhered opsonized and nonopsonized S. aureus ATCC 25923 on titanium coupons after coculture with HL-60 cells for 24 h. The left half of the graph includes the nonopsonized S. aureus ATCC 25923 biofilm formation, in absence (black column) or cocultured with HL-60 cells (green column). The right half includes the opsonized S. aureus ATCC 25923 biofilm formation, in absence (white/black column) or cocultured with HL-60 cells (green/black column). “*” indicates statistical differences with the nonopsonized S. aureus ATCC 25923 in monoculture, while “#” represents statistical differences with the opsonized S. aureus ATCC 25923 in monoculture with Welch’s unpaired t -test ( *** p
    Figure Legend Snippet: Viable counts of adhered opsonized and nonopsonized S. aureus ATCC 25923 on titanium coupons after coculture with HL-60 cells for 24 h. The left half of the graph includes the nonopsonized S. aureus ATCC 25923 biofilm formation, in absence (black column) or cocultured with HL-60 cells (green column). The right half includes the opsonized S. aureus ATCC 25923 biofilm formation, in absence (white/black column) or cocultured with HL-60 cells (green/black column). “*” indicates statistical differences with the nonopsonized S. aureus ATCC 25923 in monoculture, while “#” represents statistical differences with the opsonized S. aureus ATCC 25923 in monoculture with Welch’s unpaired t -test ( *** p

    Techniques Used:

    35) Product Images from "Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation"

    Article Title: Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation

    Journal: Microorganisms

    doi: 10.3390/microorganisms8111757

    Viable counts of adhered S. aureus ATCC 25923 ( a ) and S. aureus P2 ( b ) on titanium coupons exposed to different antimicrobial compounds (tested at 50 µM) after 24 h of incubation. The gray bars show the attached viable bacteria when exposed just to the antimicrobial compounds, while the green bars show the results when S. aureus strains were cocultured with HL-60 cells. “*” indicates statistical differences with the control in monoculture while “#” represents statistical differences with the control in cocultured controls with Welch’s unpaired t -test ( * p
    Figure Legend Snippet: Viable counts of adhered S. aureus ATCC 25923 ( a ) and S. aureus P2 ( b ) on titanium coupons exposed to different antimicrobial compounds (tested at 50 µM) after 24 h of incubation. The gray bars show the attached viable bacteria when exposed just to the antimicrobial compounds, while the green bars show the results when S. aureus strains were cocultured with HL-60 cells. “*” indicates statistical differences with the control in monoculture while “#” represents statistical differences with the control in cocultured controls with Welch’s unpaired t -test ( * p

    Techniques Used: Incubation

    Viable counts of 24-hour-old biofilms formed by different concentrations of S. aureus ATCC 25923 cocultured with HL-60 cells on 96-well microplates. Black columns represent the bacterial control (viable attached cells in the absence of HL-60 cells). Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA). Values are means and SD of three independent experiments ( *** p
    Figure Legend Snippet: Viable counts of 24-hour-old biofilms formed by different concentrations of S. aureus ATCC 25923 cocultured with HL-60 cells on 96-well microplates. Black columns represent the bacterial control (viable attached cells in the absence of HL-60 cells). Green columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with N , N -dimethylformamide (DMF). Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA). Values are means and SD of three independent experiments ( *** p

    Techniques Used:

    Viable counts of adhered S. aureus ATCC 25923 on LDPE tubes exposed to different antimicrobial compounds (tested at 50 µM) after 24 h of incubation. The gray bars show the attached viable bacteria when exposed just to the antimicrobial compounds, while the green bars show the results when S. aureus strains were cocultured with HL-60 cells. “*” indicates statistical differences with the control in monoculture, while “#” represents statistical differences with the control in cocultured controls with Welch’s unpaired t -test ( ** p
    Figure Legend Snippet: Viable counts of adhered S. aureus ATCC 25923 on LDPE tubes exposed to different antimicrobial compounds (tested at 50 µM) after 24 h of incubation. The gray bars show the attached viable bacteria when exposed just to the antimicrobial compounds, while the green bars show the results when S. aureus strains were cocultured with HL-60 cells. “*” indicates statistical differences with the control in monoculture, while “#” represents statistical differences with the control in cocultured controls with Welch’s unpaired t -test ( ** p

    Techniques Used: Incubation

    Representative images acquired by SEM. From left to right the same section of the titanium coupon is shown with different magnification, 500, 1000 and 3000×. Upper row of images, bacterial control, i.e., coupons incubated with S. aureus ATCC 25923 only. Second row, titanium coupons incubated with S. aureus ATCC 25923 and treated with DHA1 . Third row, cocultured control, titanium coupons incubated with S. aureus ATCC 25923 and HL-60 cells simultaneously. Fourth row, titanium coupons cocultured with S. aureus ATCC 25923 and HL-60 and treated with DHA1 .
    Figure Legend Snippet: Representative images acquired by SEM. From left to right the same section of the titanium coupon is shown with different magnification, 500, 1000 and 3000×. Upper row of images, bacterial control, i.e., coupons incubated with S. aureus ATCC 25923 only. Second row, titanium coupons incubated with S. aureus ATCC 25923 and treated with DHA1 . Third row, cocultured control, titanium coupons incubated with S. aureus ATCC 25923 and HL-60 cells simultaneously. Fourth row, titanium coupons cocultured with S. aureus ATCC 25923 and HL-60 and treated with DHA1 .

    Techniques Used: Incubation

    Viable counts of adhered opsonized and nonopsonized S. aureus ATCC 25923 on titanium coupons after coculture with HL-60 cells for 24 h. The left half of the graph includes the nonopsonized S. aureus ATCC 25923 biofilm formation, in absence (black column) or cocultured with HL-60 cells (green column). The right half includes the opsonized S. aureus ATCC 25923 biofilm formation, in absence (white/black column) or cocultured with HL-60 cells (green/black column). “*” indicates statistical differences with the nonopsonized S. aureus ATCC 25923 in monoculture, while “#” represents statistical differences with the opsonized S. aureus ATCC 25923 in monoculture with Welch’s unpaired t -test ( *** p
    Figure Legend Snippet: Viable counts of adhered opsonized and nonopsonized S. aureus ATCC 25923 on titanium coupons after coculture with HL-60 cells for 24 h. The left half of the graph includes the nonopsonized S. aureus ATCC 25923 biofilm formation, in absence (black column) or cocultured with HL-60 cells (green column). The right half includes the opsonized S. aureus ATCC 25923 biofilm formation, in absence (white/black column) or cocultured with HL-60 cells (green/black column). “*” indicates statistical differences with the nonopsonized S. aureus ATCC 25923 in monoculture, while “#” represents statistical differences with the opsonized S. aureus ATCC 25923 in monoculture with Welch’s unpaired t -test ( *** p

    Techniques Used:

    36) Product Images from "Satureja montana L. and Origanum majorana L. Decoctions: Antimicrobial Activity, Mode of Action and Phenolic Characterization"

    Article Title: Satureja montana L. and Origanum majorana L. Decoctions: Antimicrobial Activity, Mode of Action and Phenolic Characterization

    Journal: Antibiotics

    doi: 10.3390/antibiotics9060294

    Membrane integrity of S. aureus ATCC 25923 after 3, 5, and 24 h exposure to Satureja montana and Origanum majorana decoctions at 1.56 mg/mL. White bars with dots represent cell fragments or unstained cells; white bars represent cells with intact cell membranes; Gray bars represent cells with slight cell membrane permeabilization; Black bars represent cells with (complete) cell membrane permeabilization. The means ± standard deviations for three independent assays are presented.
    Figure Legend Snippet: Membrane integrity of S. aureus ATCC 25923 after 3, 5, and 24 h exposure to Satureja montana and Origanum majorana decoctions at 1.56 mg/mL. White bars with dots represent cell fragments or unstained cells; white bars represent cells with intact cell membranes; Gray bars represent cells with slight cell membrane permeabilization; Black bars represent cells with (complete) cell membrane permeabilization. The means ± standard deviations for three independent assays are presented.

    Techniques Used:

    37) Product Images from "Synthesis of Degraded Limonoid Analogs as New Antibacterial Scaffolds against Staphylococcus aureus"

    Article Title: Synthesis of Degraded Limonoid Analogs as New Antibacterial Scaffolds against Staphylococcus aureus

    Journal: Antibiotics

    doi: 10.3390/antibiotics9080488

    ( a ) Time-kill curve of  S. aureus  ATCC 25923; ( b ) Time-kill curve of MRSA 18032913.
    Figure Legend Snippet: ( a ) Time-kill curve of S. aureus ATCC 25923; ( b ) Time-kill curve of MRSA 18032913.

    Techniques Used:

    38) Product Images from "Synthesis of Degraded Limonoid Analogs as New Antibacterial Scaffolds against Staphylococcus aureus"

    Article Title: Synthesis of Degraded Limonoid Analogs as New Antibacterial Scaffolds against Staphylococcus aureus

    Journal: Antibiotics

    doi: 10.3390/antibiotics9080488

    ( a ) Time-kill curve of  S. aureus  ATCC 25923; ( b ) Time-kill curve of MRSA 18032913.
    Figure Legend Snippet: ( a ) Time-kill curve of S. aureus ATCC 25923; ( b ) Time-kill curve of MRSA 18032913.

    Techniques Used:

    39) Product Images from "N-terminal Myristoylation Enhanced the Antimicrobial Activity of Antimicrobial Peptide PMAP-36PW"

    Article Title: N-terminal Myristoylation Enhanced the Antimicrobial Activity of Antimicrobial Peptide PMAP-36PW

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2020.00450

    Therapeutic effect for pneumonia by Myr-36PW in vivo . Mice were injected with S. aureus ATCC 25923 or P. aeruginosa GIM 1.551 to form pneumonia, and lungs ( n = 5) in each group were collected on day 4 after intranasal bacterial inoculation. (A) Morphological changes of lung tissues. (B) Representative histopathological images of processed lung section with hematoxylin and eosin (H E) staining. Images are presented at a magnification of 20×. a: inflammatory cell infiltration. b: the alveolar wall is thickened. c: alveolar wall congestion or alveolar hemorrhage. (C,D) Pathological scores of the lung sections. Scoring standard: 1 indicated no pathology, 2 indicated local pulmonary congestion and inflammatory cell infiltration, 3 indicated pulmonary congestion and a large number of inflammatory cell infiltration, affecting
    Figure Legend Snippet: Therapeutic effect for pneumonia by Myr-36PW in vivo . Mice were injected with S. aureus ATCC 25923 or P. aeruginosa GIM 1.551 to form pneumonia, and lungs ( n = 5) in each group were collected on day 4 after intranasal bacterial inoculation. (A) Morphological changes of lung tissues. (B) Representative histopathological images of processed lung section with hematoxylin and eosin (H E) staining. Images are presented at a magnification of 20×. a: inflammatory cell infiltration. b: the alveolar wall is thickened. c: alveolar wall congestion or alveolar hemorrhage. (C,D) Pathological scores of the lung sections. Scoring standard: 1 indicated no pathology, 2 indicated local pulmonary congestion and inflammatory cell infiltration, 3 indicated pulmonary congestion and a large number of inflammatory cell infiltration, affecting

    Techniques Used: In Vivo, Mouse Assay, Injection, Staining

    Therapeutic potency of peritonitis by Myr-36PW  in vivo . The livers and spleens ( n  = 5) in each group were collected on day 4 after the intraperitoneal injection of  S. aureus  ATCC 25923.  (A)  Morphological changes in liver and spleen tissues.  (B)  Representative histopathological images of processed liver and spleen sections with hematoxylin and eosin (H  E) staining. Images are presented at a magnification of 40×. a: central venous hemorrhage. b: the cells fuse into pieces, and the nucleus dissolves and disappears. c: cytoplasmic cavitation.  (C,D)  Pathological scores of the liver and spleen sections. Scoring standard: 1 indicated no pathology, 2 indicated perivascular infiltrates, 3 indicated perivascular or interstitial infiltrates affecting
    Figure Legend Snippet: Therapeutic potency of peritonitis by Myr-36PW in vivo . The livers and spleens ( n = 5) in each group were collected on day 4 after the intraperitoneal injection of S. aureus ATCC 25923. (A) Morphological changes in liver and spleen tissues. (B) Representative histopathological images of processed liver and spleen sections with hematoxylin and eosin (H E) staining. Images are presented at a magnification of 40×. a: central venous hemorrhage. b: the cells fuse into pieces, and the nucleus dissolves and disappears. c: cytoplasmic cavitation. (C,D) Pathological scores of the liver and spleen sections. Scoring standard: 1 indicated no pathology, 2 indicated perivascular infiltrates, 3 indicated perivascular or interstitial infiltrates affecting

    Techniques Used: In Vivo, Injection, Staining

    Treatment efficacy of subcutaneous abscess by Myr-36PW in vivo . Mice were injected with S. aureus ATCC 25923 or P. aeruginosa GIM 1.551 to develop a subcutaneous abscess before treatment. (A) Changes in the abscess area of mice after 3 days treatment. (B,C) Quantitative analysis of bacterial CFU in abscess area that received 3 days treatment under various experimental conditions. The number of CFU in each treatment group was normalized by using the CFU of the experimental group divided by the CFU of the PBS group (100%). BP is benzylpenicillin potassium, AS is ampicillin sodium. Data represent the mean ± SD of five individual experiments. * P
    Figure Legend Snippet: Treatment efficacy of subcutaneous abscess by Myr-36PW in vivo . Mice were injected with S. aureus ATCC 25923 or P. aeruginosa GIM 1.551 to develop a subcutaneous abscess before treatment. (A) Changes in the abscess area of mice after 3 days treatment. (B,C) Quantitative analysis of bacterial CFU in abscess area that received 3 days treatment under various experimental conditions. The number of CFU in each treatment group was normalized by using the CFU of the experimental group divided by the CFU of the PBS group (100%). BP is benzylpenicillin potassium, AS is ampicillin sodium. Data represent the mean ± SD of five individual experiments. * P

    Techniques Used: In Vivo, Mouse Assay, Injection

    40) Product Images from "N-terminal Myristoylation Enhanced the Antimicrobial Activity of Antimicrobial Peptide PMAP-36PW"

    Article Title: N-terminal Myristoylation Enhanced the Antimicrobial Activity of Antimicrobial Peptide PMAP-36PW

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2020.00450

    Therapeutic effect for pneumonia by Myr-36PW in vivo . Mice were injected with S. aureus ATCC 25923 or P. aeruginosa GIM 1.551 to form pneumonia, and lungs ( n = 5) in each group were collected on day 4 after intranasal bacterial inoculation. (A) Morphological changes of lung tissues. (B) Representative histopathological images of processed lung section with hematoxylin and eosin (H E) staining. Images are presented at a magnification of 20×. a: inflammatory cell infiltration. b: the alveolar wall is thickened. c: alveolar wall congestion or alveolar hemorrhage. (C,D) Pathological scores of the lung sections. Scoring standard: 1 indicated no pathology, 2 indicated local pulmonary congestion and inflammatory cell infiltration, 3 indicated pulmonary congestion and a large number of inflammatory cell infiltration, affecting
    Figure Legend Snippet: Therapeutic effect for pneumonia by Myr-36PW in vivo . Mice were injected with S. aureus ATCC 25923 or P. aeruginosa GIM 1.551 to form pneumonia, and lungs ( n = 5) in each group were collected on day 4 after intranasal bacterial inoculation. (A) Morphological changes of lung tissues. (B) Representative histopathological images of processed lung section with hematoxylin and eosin (H E) staining. Images are presented at a magnification of 20×. a: inflammatory cell infiltration. b: the alveolar wall is thickened. c: alveolar wall congestion or alveolar hemorrhage. (C,D) Pathological scores of the lung sections. Scoring standard: 1 indicated no pathology, 2 indicated local pulmonary congestion and inflammatory cell infiltration, 3 indicated pulmonary congestion and a large number of inflammatory cell infiltration, affecting

    Techniques Used: In Vivo, Mouse Assay, Injection, Staining

    Therapeutic potency of peritonitis by Myr-36PW  in vivo . The livers and spleens ( n  = 5) in each group were collected on day 4 after the intraperitoneal injection of  S. aureus  ATCC 25923.  (A)  Morphological changes in liver and spleen tissues.  (B)  Representative histopathological images of processed liver and spleen sections with hematoxylin and eosin (H  E) staining. Images are presented at a magnification of 40×. a: central venous hemorrhage. b: the cells fuse into pieces, and the nucleus dissolves and disappears. c: cytoplasmic cavitation.  (C,D)  Pathological scores of the liver and spleen sections. Scoring standard: 1 indicated no pathology, 2 indicated perivascular infiltrates, 3 indicated perivascular or interstitial infiltrates affecting
    Figure Legend Snippet: Therapeutic potency of peritonitis by Myr-36PW in vivo . The livers and spleens ( n = 5) in each group were collected on day 4 after the intraperitoneal injection of S. aureus ATCC 25923. (A) Morphological changes in liver and spleen tissues. (B) Representative histopathological images of processed liver and spleen sections with hematoxylin and eosin (H E) staining. Images are presented at a magnification of 40×. a: central venous hemorrhage. b: the cells fuse into pieces, and the nucleus dissolves and disappears. c: cytoplasmic cavitation. (C,D) Pathological scores of the liver and spleen sections. Scoring standard: 1 indicated no pathology, 2 indicated perivascular infiltrates, 3 indicated perivascular or interstitial infiltrates affecting

    Techniques Used: In Vivo, Injection, Staining

    Treatment efficacy of subcutaneous abscess by Myr-36PW in vivo . Mice were injected with S. aureus ATCC 25923 or P. aeruginosa GIM 1.551 to develop a subcutaneous abscess before treatment. (A) Changes in the abscess area of mice after 3 days treatment. (B,C) Quantitative analysis of bacterial CFU in abscess area that received 3 days treatment under various experimental conditions. The number of CFU in each treatment group was normalized by using the CFU of the experimental group divided by the CFU of the PBS group (100%). BP is benzylpenicillin potassium, AS is ampicillin sodium. Data represent the mean ± SD of five individual experiments. * P
    Figure Legend Snippet: Treatment efficacy of subcutaneous abscess by Myr-36PW in vivo . Mice were injected with S. aureus ATCC 25923 or P. aeruginosa GIM 1.551 to develop a subcutaneous abscess before treatment. (A) Changes in the abscess area of mice after 3 days treatment. (B,C) Quantitative analysis of bacterial CFU in abscess area that received 3 days treatment under various experimental conditions. The number of CFU in each treatment group was normalized by using the CFU of the experimental group divided by the CFU of the PBS group (100%). BP is benzylpenicillin potassium, AS is ampicillin sodium. Data represent the mean ± SD of five individual experiments. * P

    Techniques Used: In Vivo, Mouse Assay, Injection

    Related Articles

    Sequencing:

    Article Title: Molecular Characterization of a Novel Lytic Enzyme LysC from Clostridium intestinale URNW and Its Antibacterial Activity Mediated by Positively Charged N-Terminal Extension
    Article Snippet: .. A 30 amino acids peptide, which we termed Intestinalin, designed and synthetized based on the sequence of the N -terminal region of LysC, was shown to have high antibacterial activity against S. aureus ATCC 25923. .. A 30 amino acids peptide, which we termed Intestinalin, designed and synthetized based on the sequence of the N -terminal region of LysC, was shown to have high antibacterial activity against S. aureus ATCC 25923.

    Concentration Assay:

    Article Title: Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
    Article Snippet: .. The suspension of approx. cell density 1.5 × 106 CFU/mL of S. aureus ATCC 25923 was prepared in MHB2 broth supplemented with honey to the final concentration equal to MBC or 2 × MBC and incubated at 37 °C with shaking. .. Viable counts of the cells in the suspensions were obtained from 10-fold serial dilutions at 0, 1, 2, 4, 6, 8 and 24 h by plating 10-fold dilution on Baird-Parker agar plates and incubating at 37 °C for 24 h. The number of the cells in the control suspension, without honey addition, was also determined as a control of growth kinetic of S. aureus ATCC 25923.

    Article Title: Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils
    Article Snippet: .. Biofilm eradication effect was observed for S. aureus ATCC 25923 and P. aeruginosa ATCC 27853 at lower concentration than for E. coli ATCC 25922 and C. albicans ATCC 10231. ..

    Article Title: Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
    Article Snippet: .. However, the complete elimination of living cells of S. aureus ATCC 25923 required extended incubation with honeys (both Polish honeys and Man550 honey) up to 24 h. Nearly the same effect was observed when the honeys were used at the concentration equal to the MBC value or 2 × MBC. .. Interestingly, both honeys collected in Polish apiaries (assigned as 35 and 86) revealed slightly higher activity in comparison to Man550 during the first eight hours of incubation.

    Incubation:

    Article Title: Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
    Article Snippet: .. The suspension of approx. cell density 1.5 × 106 CFU/mL of S. aureus ATCC 25923 was prepared in MHB2 broth supplemented with honey to the final concentration equal to MBC or 2 × MBC and incubated at 37 °C with shaking. .. Viable counts of the cells in the suspensions were obtained from 10-fold serial dilutions at 0, 1, 2, 4, 6, 8 and 24 h by plating 10-fold dilution on Baird-Parker agar plates and incubating at 37 °C for 24 h. The number of the cells in the control suspension, without honey addition, was also determined as a control of growth kinetic of S. aureus ATCC 25923.

    Article Title: Paromomycin production from Streptomyces rimosus NRRL 2455: statistical optimization and new synergistic antibiotic combinations against multidrug resistant pathogens
    Article Snippet: .. Using Response Surface Methodology, other factors including, pH, inoculum size and incubation period were optimized using the statistical software package Design Expert v. 7.0 (Design Expert Software, Stat-Ease Inc., Statistics Made Easy, Minneapolis, MN, USA).At the end of each run the antimicrobial activity of paromomycin was bioassayed against Staphylococcus aureus ATCC 25923. ..

    Article Title: Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
    Article Snippet: .. However, the complete elimination of living cells of S. aureus ATCC 25923 required extended incubation with honeys (both Polish honeys and Man550 honey) up to 24 h. Nearly the same effect was observed when the honeys were used at the concentration equal to the MBC value or 2 × MBC. .. Interestingly, both honeys collected in Polish apiaries (assigned as 35 and 86) revealed slightly higher activity in comparison to Man550 during the first eight hours of incubation.

    Activity Assay:

    Article Title: Paromomycin production from Streptomyces rimosus NRRL 2455: statistical optimization and new synergistic antibiotic combinations against multidrug resistant pathogens
    Article Snippet: .. Using Response Surface Methodology, other factors including, pH, inoculum size and incubation period were optimized using the statistical software package Design Expert v. 7.0 (Design Expert Software, Stat-Ease Inc., Statistics Made Easy, Minneapolis, MN, USA).At the end of each run the antimicrobial activity of paromomycin was bioassayed against Staphylococcus aureus ATCC 25923. ..

    Article Title: Molecular Characterization of a Novel Lytic Enzyme LysC from Clostridium intestinale URNW and Its Antibacterial Activity Mediated by Positively Charged N-Terminal Extension
    Article Snippet: .. A 30 amino acids peptide, which we termed Intestinalin, designed and synthetized based on the sequence of the N -terminal region of LysC, was shown to have high antibacterial activity against S. aureus ATCC 25923. .. A 30 amino acids peptide, which we termed Intestinalin, designed and synthetized based on the sequence of the N -terminal region of LysC, was shown to have high antibacterial activity against S. aureus ATCC 25923.

    Software:

    Article Title: Paromomycin production from Streptomyces rimosus NRRL 2455: statistical optimization and new synergistic antibiotic combinations against multidrug resistant pathogens
    Article Snippet: .. Using Response Surface Methodology, other factors including, pH, inoculum size and incubation period were optimized using the statistical software package Design Expert v. 7.0 (Design Expert Software, Stat-Ease Inc., Statistics Made Easy, Minneapolis, MN, USA).At the end of each run the antimicrobial activity of paromomycin was bioassayed against Staphylococcus aureus ATCC 25923. ..

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    ATCC s aureus atcc 25923
    Time-kill curves of S. aureus <t>ATCC</t> 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.
    S Aureus Atcc 25923, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Time-kill curves of S. aureus ATCC 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland

    doi: 10.3390/molecules23020260

    Figure Lengend Snippet: Time-kill curves of S. aureus ATCC 25923 cultivated in absence or presence of two the most active honeys: ( a ) multi-floral honey and ( b ) buckwheat honey, both collected by professional beekeepers; in comparison to manuka honey ( c ). Concentrations of honeys are presented in relation to MBC determined.

    Article Snippet: However, the complete elimination of living cells of S. aureus ATCC 25923 required extended incubation with honeys (both Polish honeys and Man550 honey) up to 24 h. Nearly the same effect was observed when the honeys were used at the concentration equal to the MBC value or 2 × MBC.

    Techniques:

    Antibacterial activity of AgNPs against different bacteria ( E .  coli  ATCC 25922,  S. aureus  ATCC 25923,  B. subtilis ,  P. aeruginosa ,  K. pneumoniae  AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.

    Journal: Scientific Reports

    Article Title: Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria

    doi: 10.1038/s41598-017-18590-6

    Figure Lengend Snippet: Antibacterial activity of AgNPs against different bacteria ( E . coli ATCC 25922, S. aureus ATCC 25923, B. subtilis , P. aeruginosa , K. pneumoniae AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.

    Article Snippet: Similarly, the MIC values of AgNP-sp and AgNR for E. coli ATCC 25922 was 190 µg/ml and 340 µg/ml, for Pseudomonas aeruginosa AL2-14B 188 µg/ml and 348 µg/ml and for S. aureus ATCC 25923 was 190 µg/ml and 358 µg/ml, for B. subtilis AST5-2 was 195 µg/ml and 350 µg/ml respectively.

    Techniques: Activity Assay

    Influence of moxifloxacin on the viability of S. aureus ATCC 25923 using inocula of 10 5 (A) and 10 7 CFU/ml (B). The antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in two determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter; ×, 0.1 mg/liter ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Discrepancy between Uptake and Intracellular Activity of Moxifloxacin in a Staphylococcus aureus-Human THP-1 Monocytic Cell Model

    doi: 10.1128/AAC.46.2.288-293.2002

    Figure Lengend Snippet: Influence of moxifloxacin on the viability of S. aureus ATCC 25923 using inocula of 10 5 (A) and 10 7 CFU/ml (B). The antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in two determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter; ×, 0.1 mg/liter ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.

    Article Snippet: At concentrations ≤0.2 mg/liter, the drug inhibited only the intracellular bacterial growth, while at concentrations ≥0.5 mg/liter, it decreased the bacterial inoculum by less than 1 log10 unit, with a maximum effect at 3 to 4 h, followed by regrowth of surviving bacteria to 80 to 120% of the original level at 5 h. In contrast, when killing curves were determined by using Mueller-Hinton broth with a similar inoculum (107 CFU/ml), moxifloxacin at concentrations ≥0.2 mg/liter reduced the inoculum by at least 3 log10 units at 3 to 4 h, leaving ≤0.1% survival at 24 h. Persisters exhibited a fluoroquinolone susceptibility identical to that of S. aureus ATCC 25923.

    Techniques:

    Effect of moxifloxacin on the viability of S. aureus ATCC 25923 ingested by THP-1 cells. Antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in three determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter, ×, 0.1 mg/liter; ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Discrepancy between Uptake and Intracellular Activity of Moxifloxacin in a Staphylococcus aureus-Human THP-1 Monocytic Cell Model

    doi: 10.1128/AAC.46.2.288-293.2002

    Figure Lengend Snippet: Effect of moxifloxacin on the viability of S. aureus ATCC 25923 ingested by THP-1 cells. Antibacterial effect of moxifloxacin was expressed as the mean number of viable bacteria obtained in three determinations. This was normalized for the control growth curve to allow comparison between antibiotic concentrations. Symbols: ▪, control; ◊, 0.06 mg/liter, ×, 0.1 mg/liter; ⧫, 0.2 mg/liter; *, 0.5 mg/liter; □, 1 mg/liter; ○, 2 mg/liter; ▴, 4 mg/liter; ▵, 8 mg/liter.

    Article Snippet: At concentrations ≤0.2 mg/liter, the drug inhibited only the intracellular bacterial growth, while at concentrations ≥0.5 mg/liter, it decreased the bacterial inoculum by less than 1 log10 unit, with a maximum effect at 3 to 4 h, followed by regrowth of surviving bacteria to 80 to 120% of the original level at 5 h. In contrast, when killing curves were determined by using Mueller-Hinton broth with a similar inoculum (107 CFU/ml), moxifloxacin at concentrations ≥0.2 mg/liter reduced the inoculum by at least 3 log10 units at 3 to 4 h, leaving ≤0.1% survival at 24 h. Persisters exhibited a fluoroquinolone susceptibility identical to that of S. aureus ATCC 25923.

    Techniques: