ryr3  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ryr3
    The ryanodine receptor <t>(RyR)1/RyR3</t> inhibitor dantrolene does not inhibit rat mesenteric collecting lymphatic contractions or the increased CF and decreased EDD/Max elicited by substance P (SP). Representative trace of diameter over time of an isolated rat mesenteric collecting lymphatic vessel before and after the addition of 10 −5 M dantrolene, which remained in the bath ( A ). Continuation of the same trace, showing the addition of 10 −8 M SP in the presence of dantrolene ( B ). The boxes beneath the traces in A and B indicate 2-min time periods during which the quantitative data used for comparisons in C – H were collected. Comparisons between baseline (BL), after addition of dantrolene, and after addition of SP in the presence of dantrolene were performed for CF ( C ), EDD/MaxD ( D ), ESD/MaxD ( E ), AMP/MaxD ( F ), EF ( G ), and FPF ( H ). P values are shown for comparisons that were found to be significantly different ( P
    Ryr3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ryr3/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ryr3 - by Bioz Stars, 2022-12
    95/100 stars

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    1) Product Images from "Evidence of functional ryanodine receptors in rat mesenteric collecting lymphatic vessels"

    Article Title: Evidence of functional ryanodine receptors in rat mesenteric collecting lymphatic vessels

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00564.2018

    The ryanodine receptor (RyR)1/RyR3 inhibitor dantrolene does not inhibit rat mesenteric collecting lymphatic contractions or the increased CF and decreased EDD/Max elicited by substance P (SP). Representative trace of diameter over time of an isolated rat mesenteric collecting lymphatic vessel before and after the addition of 10 −5 M dantrolene, which remained in the bath ( A ). Continuation of the same trace, showing the addition of 10 −8 M SP in the presence of dantrolene ( B ). The boxes beneath the traces in A and B indicate 2-min time periods during which the quantitative data used for comparisons in C – H were collected. Comparisons between baseline (BL), after addition of dantrolene, and after addition of SP in the presence of dantrolene were performed for CF ( C ), EDD/MaxD ( D ), ESD/MaxD ( E ), AMP/MaxD ( F ), EF ( G ), and FPF ( H ). P values are shown for comparisons that were found to be significantly different ( P
    Figure Legend Snippet: The ryanodine receptor (RyR)1/RyR3 inhibitor dantrolene does not inhibit rat mesenteric collecting lymphatic contractions or the increased CF and decreased EDD/Max elicited by substance P (SP). Representative trace of diameter over time of an isolated rat mesenteric collecting lymphatic vessel before and after the addition of 10 −5 M dantrolene, which remained in the bath ( A ). Continuation of the same trace, showing the addition of 10 −8 M SP in the presence of dantrolene ( B ). The boxes beneath the traces in A and B indicate 2-min time periods during which the quantitative data used for comparisons in C – H were collected. Comparisons between baseline (BL), after addition of dantrolene, and after addition of SP in the presence of dantrolene were performed for CF ( C ), EDD/MaxD ( D ), ESD/MaxD ( E ), AMP/MaxD ( F ), EF ( G ), and FPF ( H ). P values are shown for comparisons that were found to be significantly different ( P

    Techniques Used: Isolation

    Blockade of ryanodine receptors (RyRs) with ryanodine leads to a loss of phasic contractions and limited substance P (SP)-induced contraction. Representative trace of diameter over time of an isolated rat collecting mesenteric lymphatic vessel treated with 10 −5  M ryanodine ( A ). The continuation of the trace shows a later time period in the same experiment with ryanodine, when 10 −8  M SP was added. The boxes beneath the trace represent the time periods during which the quantitative data used for comparisons in  B – D  were collected. The mean diameter ( B ), mean tone ( C ), and maximum tone ( D ) for the time periods before addition of SP, 0–30 s after SP addition, and 31–150 s after addition of SP were compared using a repeated measures ANOVA model followed by Dunnett’s comparison with control (baseline period).  P  values for the comparisons are shown.  N  = 6 lymphatics. Each lymphatic studied was obtained from a unique rat. NS, not significant.
    Figure Legend Snippet: Blockade of ryanodine receptors (RyRs) with ryanodine leads to a loss of phasic contractions and limited substance P (SP)-induced contraction. Representative trace of diameter over time of an isolated rat collecting mesenteric lymphatic vessel treated with 10 −5 M ryanodine ( A ). The continuation of the trace shows a later time period in the same experiment with ryanodine, when 10 −8 M SP was added. The boxes beneath the trace represent the time periods during which the quantitative data used for comparisons in B – D were collected. The mean diameter ( B ), mean tone ( C ), and maximum tone ( D ) for the time periods before addition of SP, 0–30 s after SP addition, and 31–150 s after addition of SP were compared using a repeated measures ANOVA model followed by Dunnett’s comparison with control (baseline period). P values for the comparisons are shown. N = 6 lymphatics. Each lymphatic studied was obtained from a unique rat. NS, not significant.

    Techniques Used: Isolation

    Quantitative PCR (qPCR) detection of ryanodine receptors (RyRs) in rat mesenteric collecting lymphatic vessels. A : means show relative expression to GAPDH determined by qPCR and using the 2 -ΔCt method in isolated rat mesenteric collecting lymphatics ( N = 7 rats) and rat brain, skeletal muscle, and cardiac muscle (all N = 2 rats). B : gel of the single amplicon products of expected size from the qPCR reactions. Rat mesenteric collecting lymphatics were harvested (30 lymphatic vessels per rat), and total RNA (50 ng) was reverse transcribed into cDNA and qPCR performed with specific primer/probe sets for Ryr1 (Rn01545085_m1), Ryr2 (Rn01470303_m1), Ryr3 (Rn01486097_m1), and GAPDH (Rn01775763_g1). Reactions with no template were also run as controls (NTC). The gel shown is representative of three separate experiments using mesenteric lymphatic samples obtained from three different rats.
    Figure Legend Snippet: Quantitative PCR (qPCR) detection of ryanodine receptors (RyRs) in rat mesenteric collecting lymphatic vessels. A : means show relative expression to GAPDH determined by qPCR and using the 2 -ΔCt method in isolated rat mesenteric collecting lymphatics ( N = 7 rats) and rat brain, skeletal muscle, and cardiac muscle (all N = 2 rats). B : gel of the single amplicon products of expected size from the qPCR reactions. Rat mesenteric collecting lymphatics were harvested (30 lymphatic vessels per rat), and total RNA (50 ng) was reverse transcribed into cDNA and qPCR performed with specific primer/probe sets for Ryr1 (Rn01545085_m1), Ryr2 (Rn01470303_m1), Ryr3 (Rn01486097_m1), and GAPDH (Rn01775763_g1). Reactions with no template were also run as controls (NTC). The gel shown is representative of three separate experiments using mesenteric lymphatic samples obtained from three different rats.

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Isolation, Amplification

    Determination of ryanodine receptor (RyR)2 and RyR3 localization in isolated rat mesenteric collecting lymphatic vessels with immunofluorescence labeling and laser confocal microscopy. A . maximum intensity z-projection (37 confocal slices) of a lymphatic vessel labeled with anti-RyR2 AlexaFluor488-conjugated secondary antibodies and DAPI. The RyR2 labeling appeared mostly in hoop-like patterns in the vessel wall, with some labeling in the adventitial layer (1 example denoted by the small white arrow). B : maximum intensity z-projection (85 confocal slices) of a lymphatic immunolabeled for RyR3 and nuclei. A similar hoop-like pattern was observed with RyR3 immunolabeling, with some adventitial layer labeling (small white arrow). No signal was detected in labeling controls with no primary antibody (data not shown). C and E : labeling of RyR2, smooth muscle actin (SMA), nuclei, and a merge of the three channels in a single confocal slice featuring a cross-section of the collecting lymphatic wall. The orientation is with the adventitial layer on the left and the endothelial layer on the right. RyR2 labeling was predominantly found in the smooth muscle layer, as evidenced by partial colocalization with SMA and RyR2 labeling surrounding the nuclei of smooth muscle cells. D and F : labeling of RyR3, SMA, nuclei, and a merge of the three channels in a single confocal slice featuring a cross-section of the lymphatic wall, oriented with the adventitial layer on the left and endothelium on the right. RyR3 was predominantly found in smooth muscle cells, as evidenced by the partial colocalization with SMA. The small arrows in E and F point toward nuclei of endothelial cells (ECs). A and B : representative of N = 8 labeling experiments each. C – F : representative of N = 4 experiments each. The scale bars in C – F represent 5 µm.
    Figure Legend Snippet: Determination of ryanodine receptor (RyR)2 and RyR3 localization in isolated rat mesenteric collecting lymphatic vessels with immunofluorescence labeling and laser confocal microscopy. A . maximum intensity z-projection (37 confocal slices) of a lymphatic vessel labeled with anti-RyR2 AlexaFluor488-conjugated secondary antibodies and DAPI. The RyR2 labeling appeared mostly in hoop-like patterns in the vessel wall, with some labeling in the adventitial layer (1 example denoted by the small white arrow). B : maximum intensity z-projection (85 confocal slices) of a lymphatic immunolabeled for RyR3 and nuclei. A similar hoop-like pattern was observed with RyR3 immunolabeling, with some adventitial layer labeling (small white arrow). No signal was detected in labeling controls with no primary antibody (data not shown). C and E : labeling of RyR2, smooth muscle actin (SMA), nuclei, and a merge of the three channels in a single confocal slice featuring a cross-section of the collecting lymphatic wall. The orientation is with the adventitial layer on the left and the endothelial layer on the right. RyR2 labeling was predominantly found in the smooth muscle layer, as evidenced by partial colocalization with SMA and RyR2 labeling surrounding the nuclei of smooth muscle cells. D and F : labeling of RyR3, SMA, nuclei, and a merge of the three channels in a single confocal slice featuring a cross-section of the lymphatic wall, oriented with the adventitial layer on the left and endothelium on the right. RyR3 was predominantly found in smooth muscle cells, as evidenced by the partial colocalization with SMA. The small arrows in E and F point toward nuclei of endothelial cells (ECs). A and B : representative of N = 8 labeling experiments each. C – F : representative of N = 4 experiments each. The scale bars in C – F represent 5 µm.

    Techniques Used: Isolation, Immunofluorescence, Labeling, Confocal Microscopy, Immunolabeling

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    Thermo Fisher ryr1
    <t>RyR1</t> but not <t>RyR2</t> or <t>RyR3</t> are expressed in fast twitch skeletal muscle. Immunoblots of extracts of Tibialis anterior muscles with antibodies to RyR1, RyR2, and RyR3. Only anti-RyR1 shows a strong band at ∼550 kDa.
    Ryr1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ryr1/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ryr1 - by Bioz Stars, 2022-12
    88/100 stars
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    RyR1 but not RyR2 or RyR3 are expressed in fast twitch skeletal muscle. Immunoblots of extracts of Tibialis anterior muscles with antibodies to RyR1, RyR2, and RyR3. Only anti-RyR1 shows a strong band at ∼550 kDa.

    Journal: Frontiers in Physiology

    Article Title: Elevated Ca2+ at the triad junction underlies dysregulation of Ca2+ signaling in dysferlin-null skeletal muscle

    doi: 10.3389/fphys.2022.1032447

    Figure Lengend Snippet: RyR1 but not RyR2 or RyR3 are expressed in fast twitch skeletal muscle. Immunoblots of extracts of Tibialis anterior muscles with antibodies to RyR1, RyR2, and RyR3. Only anti-RyR1 shows a strong band at ∼550 kDa.

    Article Snippet: As the presence of non-junctional RyR isoforms could account for CICR in A/J muscle fibers, we used immunoblotting of muscle extracts to determine if RyR2 and RyR3 were expressed together with RyR1.

    Techniques: Western Blot