anti ryanodine receptor3 antibody ryr3  (Alomone Labs)


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    Alomone Labs anti ryanodine receptor3 antibody ryr3
    Anti Ryanodine Receptor3 Antibody Ryr3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ryanodine receptor3 antibody ryr3/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ryanodine receptor3 antibody ryr3 - by Bioz Stars, 2024-06
    86/100 stars

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    ryr2  (Alomone Labs)


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    Alomone Labs ryr2
    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and <t>RyR2</t> activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.
    Ryr2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ryr2 - by Bioz Stars, 2024-06
    86/100 stars

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    1) Product Images from "Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor"

    Article Title: Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2024.101914

    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and RyR2 activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.
    Figure Legend Snippet: Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and RyR2 activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.

    Techniques Used: Activity Assay, Western Blot

    Maternal exercise protects against Ryr2 oxidation and gene methylation in embryos . A) Western blot of Ryr2 and DNP (oxidation levels) and B) quantification in embryonic day 18.5 offspring hearts. C) Methylation status of RyR2. Expression levels of the methylated RyR2 promoter normalized by the expression of unmethylated RyR2 promoter in embryonic day 18.5 and perinatal day 0 offspring. D) Schematic figure identifying a pathway for maternal exercise to blunt the effect of a maternal high-fat diet (HFD) on changes in RYR methylation and mitochondrial dysfunction leading to RyR2 oxidation in cardiomyocytes. Data presented as mean ± SEM. Embryonic day 18.5 offspring from HS n = 3, HW n = 3 for RyR2 oxidation. e18.5 offspring and perinatal day 0 offspring (PND0) from CS n = 8, HS n = 5, HW n = 11 for RyR2 methylation. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗∗ P < 0.01 vs . Offspring of CS; ## P < 0.01, ### P < 0.001 vs . offspring of HS.
    Figure Legend Snippet: Maternal exercise protects against Ryr2 oxidation and gene methylation in embryos . A) Western blot of Ryr2 and DNP (oxidation levels) and B) quantification in embryonic day 18.5 offspring hearts. C) Methylation status of RyR2. Expression levels of the methylated RyR2 promoter normalized by the expression of unmethylated RyR2 promoter in embryonic day 18.5 and perinatal day 0 offspring. D) Schematic figure identifying a pathway for maternal exercise to blunt the effect of a maternal high-fat diet (HFD) on changes in RYR methylation and mitochondrial dysfunction leading to RyR2 oxidation in cardiomyocytes. Data presented as mean ± SEM. Embryonic day 18.5 offspring from HS n = 3, HW n = 3 for RyR2 oxidation. e18.5 offspring and perinatal day 0 offspring (PND0) from CS n = 8, HS n = 5, HW n = 11 for RyR2 methylation. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗∗ P < 0.01 vs . Offspring of CS; ## P < 0.01, ### P < 0.001 vs . offspring of HS.

    Techniques Used: Methylation, Western Blot, Expressing

    anti ryr2 antibody  (Alomone Labs)


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    Alomone Labs anti ryr2 antibody
    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and <t>RyR2</t> activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.
    Anti Ryr2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ryr2 antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ryr2 antibody - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor"

    Article Title: Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2024.101914

    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and RyR2 activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.
    Figure Legend Snippet: Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and RyR2 activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.

    Techniques Used: Activity Assay, Western Blot

    Maternal exercise protects against Ryr2 oxidation and gene methylation in embryos . A) Western blot of Ryr2 and DNP (oxidation levels) and B) quantification in embryonic day 18.5 offspring hearts. C) Methylation status of RyR2. Expression levels of the methylated RyR2 promoter normalized by the expression of unmethylated RyR2 promoter in embryonic day 18.5 and perinatal day 0 offspring. D) Schematic figure identifying a pathway for maternal exercise to blunt the effect of a maternal high-fat diet (HFD) on changes in RYR methylation and mitochondrial dysfunction leading to RyR2 oxidation in cardiomyocytes. Data presented as mean ± SEM. Embryonic day 18.5 offspring from HS n = 3, HW n = 3 for RyR2 oxidation. e18.5 offspring and perinatal day 0 offspring (PND0) from CS n = 8, HS n = 5, HW n = 11 for RyR2 methylation. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗∗ P < 0.01 vs . Offspring of CS; ## P < 0.01, ### P < 0.001 vs . offspring of HS.
    Figure Legend Snippet: Maternal exercise protects against Ryr2 oxidation and gene methylation in embryos . A) Western blot of Ryr2 and DNP (oxidation levels) and B) quantification in embryonic day 18.5 offspring hearts. C) Methylation status of RyR2. Expression levels of the methylated RyR2 promoter normalized by the expression of unmethylated RyR2 promoter in embryonic day 18.5 and perinatal day 0 offspring. D) Schematic figure identifying a pathway for maternal exercise to blunt the effect of a maternal high-fat diet (HFD) on changes in RYR methylation and mitochondrial dysfunction leading to RyR2 oxidation in cardiomyocytes. Data presented as mean ± SEM. Embryonic day 18.5 offspring from HS n = 3, HW n = 3 for RyR2 oxidation. e18.5 offspring and perinatal day 0 offspring (PND0) from CS n = 8, HS n = 5, HW n = 11 for RyR2 methylation. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗∗ P < 0.01 vs . Offspring of CS; ## P < 0.01, ### P < 0.001 vs . offspring of HS.

    Techniques Used: Methylation, Western Blot, Expressing

    phospho ryr2 s2808 badrilla  (Alomone Labs)


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    Alomone Labs phospho ryr2 s2808 badrilla
    Phospho Ryr2 S2808 Badrilla, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho ryr2 s2808 badrilla/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    phospho ryr2 s2808 badrilla - by Bioz Stars, 2024-06
    86/100 stars

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    rabbit anti ryanodine receptor 2 ryr2  (Alomone Labs)


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    Alomone Labs rabbit anti ryanodine receptor 2 ryr2
    Rabbit Anti Ryanodine Receptor 2 Ryr2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ryanodine receptor 2 ryr2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    rabbit anti ryanodine receptor 2 ryr2 - by Bioz Stars, 2024-06
    86/100 stars

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    rabbit anti ryanodine receptor 2 ryr2  (Alomone Labs)


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    Alomone Labs rabbit anti ryanodine receptor 2 ryr2
    Rabbit Anti Ryanodine Receptor 2 Ryr2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ryanodine receptor 2 ryr2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    rabbit anti ryanodine receptor 2 ryr2 - by Bioz Stars, 2024-06
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    anti ryr2 antibody  (Alomone Labs)


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    Alomone Labs anti ryr2 antibody
    Anti Ryr2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ryr2 antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    anti ryr2  (Alomone Labs)


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    Alomone Labs anti ryr2
    Antibodies used in this study
    Anti Ryr2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ryr2/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ryr2 - by Bioz Stars, 2024-06
    86/100 stars

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    1) Product Images from "Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes"

    Article Title: Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes

    Journal: Basic Research in Cardiology

    doi: 10.1007/s00395-023-00988-1

    Antibodies used in this study
    Figure Legend Snippet: Antibodies used in this study

    Techniques Used: Western Blot

    COX7RP overexpression reduces spontaneous SR Ca 2+ release and mito-ROS in male ventricular myocytes under β-adrenergic stimulation. Conversely, COX7RP knock-down increases Ca 2+ waves and mito-ROS in females. A , B Representative Ca 2+ traces ( A ) and pooled data ( B ) for spontaneous Ca 2+ waves (SCW) latency in male (M), female (F), male overexpressing COX7RP (M + COX), and female expressing shRNA COX7RP (F + shC) ventricular cardiomyocytes (VCMs) exposed to 50 nmol/L isoproterenol (ISO, 5 min) after cessation of field stimulation (1 Hz). Mean ± SEM, n = 58–64, N = 6–9 preparations, * p < 0.05, p values were calculated using two-level random intercept model. C Pooled data for normalized fluorescence of mitochondria matrix ROS biosensor MLS-HyPer-7. Mean ± SEM, n = 29–42 VCMs, N = 6–9 animals, * p < 0.05, p values were calculated using two-level random intercept model. D Adenovirus–mediated expression of COX7RP in males and shRNA COX7RP in females alter RyR2 oxidation levels in rat VMs exposed to isoproterenol (ISO, 50 nmol/L) paced at 1 Hz for 5 min cultured for 48 h after infection (10 MOI). Immunoprecipitated RyR2s from M and F cultured VCM samples were probed with Anti-DNP antibody to detect amount of oxidized cysteines. E Pooled DNP optical density normalized to corresponding RyR2 signal. Mean ± SEM, N = 3 M, N = 4 F. * p < 0.05, Student’s t -test
    Figure Legend Snippet: COX7RP overexpression reduces spontaneous SR Ca 2+ release and mito-ROS in male ventricular myocytes under β-adrenergic stimulation. Conversely, COX7RP knock-down increases Ca 2+ waves and mito-ROS in females. A , B Representative Ca 2+ traces ( A ) and pooled data ( B ) for spontaneous Ca 2+ waves (SCW) latency in male (M), female (F), male overexpressing COX7RP (M + COX), and female expressing shRNA COX7RP (F + shC) ventricular cardiomyocytes (VCMs) exposed to 50 nmol/L isoproterenol (ISO, 5 min) after cessation of field stimulation (1 Hz). Mean ± SEM, n = 58–64, N = 6–9 preparations, * p < 0.05, p values were calculated using two-level random intercept model. C Pooled data for normalized fluorescence of mitochondria matrix ROS biosensor MLS-HyPer-7. Mean ± SEM, n = 29–42 VCMs, N = 6–9 animals, * p < 0.05, p values were calculated using two-level random intercept model. D Adenovirus–mediated expression of COX7RP in males and shRNA COX7RP in females alter RyR2 oxidation levels in rat VMs exposed to isoproterenol (ISO, 50 nmol/L) paced at 1 Hz for 5 min cultured for 48 h after infection (10 MOI). Immunoprecipitated RyR2s from M and F cultured VCM samples were probed with Anti-DNP antibody to detect amount of oxidized cysteines. E Pooled DNP optical density normalized to corresponding RyR2 signal. Mean ± SEM, N = 3 M, N = 4 F. * p < 0.05, Student’s t -test

    Techniques Used: Over Expression, Expressing, shRNA, Fluorescence, Cell Culture, Infection, Immunoprecipitation

    anti ryr2 antibody  (Alomone Labs)


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    Alomone Labs anti ryr2 antibody
    Antibodies used in this study
    Anti Ryr2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ryr2 antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ryr2 antibody - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes"

    Article Title: Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes

    Journal: Basic Research in Cardiology

    doi: 10.1007/s00395-023-00988-1

    Antibodies used in this study
    Figure Legend Snippet: Antibodies used in this study

    Techniques Used: Western Blot

    COX7RP overexpression reduces spontaneous SR Ca 2+ release and mito-ROS in male ventricular myocytes under β-adrenergic stimulation. Conversely, COX7RP knock-down increases Ca 2+ waves and mito-ROS in females. A , B Representative Ca 2+ traces ( A ) and pooled data ( B ) for spontaneous Ca 2+ waves (SCW) latency in male (M), female (F), male overexpressing COX7RP (M + COX), and female expressing shRNA COX7RP (F + shC) ventricular cardiomyocytes (VCMs) exposed to 50 nmol/L isoproterenol (ISO, 5 min) after cessation of field stimulation (1 Hz). Mean ± SEM, n = 58–64, N = 6–9 preparations, * p < 0.05, p values were calculated using two-level random intercept model. C Pooled data for normalized fluorescence of mitochondria matrix ROS biosensor MLS-HyPer-7. Mean ± SEM, n = 29–42 VCMs, N = 6–9 animals, * p < 0.05, p values were calculated using two-level random intercept model. D Adenovirus–mediated expression of COX7RP in males and shRNA COX7RP in females alter RyR2 oxidation levels in rat VMs exposed to isoproterenol (ISO, 50 nmol/L) paced at 1 Hz for 5 min cultured for 48 h after infection (10 MOI). Immunoprecipitated RyR2s from M and F cultured VCM samples were probed with Anti-DNP antibody to detect amount of oxidized cysteines. E Pooled DNP optical density normalized to corresponding RyR2 signal. Mean ± SEM, N = 3 M, N = 4 F. * p < 0.05, Student’s t -test
    Figure Legend Snippet: COX7RP overexpression reduces spontaneous SR Ca 2+ release and mito-ROS in male ventricular myocytes under β-adrenergic stimulation. Conversely, COX7RP knock-down increases Ca 2+ waves and mito-ROS in females. A , B Representative Ca 2+ traces ( A ) and pooled data ( B ) for spontaneous Ca 2+ waves (SCW) latency in male (M), female (F), male overexpressing COX7RP (M + COX), and female expressing shRNA COX7RP (F + shC) ventricular cardiomyocytes (VCMs) exposed to 50 nmol/L isoproterenol (ISO, 5 min) after cessation of field stimulation (1 Hz). Mean ± SEM, n = 58–64, N = 6–9 preparations, * p < 0.05, p values were calculated using two-level random intercept model. C Pooled data for normalized fluorescence of mitochondria matrix ROS biosensor MLS-HyPer-7. Mean ± SEM, n = 29–42 VCMs, N = 6–9 animals, * p < 0.05, p values were calculated using two-level random intercept model. D Adenovirus–mediated expression of COX7RP in males and shRNA COX7RP in females alter RyR2 oxidation levels in rat VMs exposed to isoproterenol (ISO, 50 nmol/L) paced at 1 Hz for 5 min cultured for 48 h after infection (10 MOI). Immunoprecipitated RyR2s from M and F cultured VCM samples were probed with Anti-DNP antibody to detect amount of oxidized cysteines. E Pooled DNP optical density normalized to corresponding RyR2 signal. Mean ± SEM, N = 3 M, N = 4 F. * p < 0.05, Student’s t -test

    Techniques Used: Over Expression, Expressing, shRNA, Fluorescence, Cell Culture, Infection, Immunoprecipitation

    ryr3  (Alomone Labs)


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    Alomone Labs ryr3
    Presence of CCNB1, c-MOS, ITPR1, and <t>RYR3</t> proteins in quail eggs and their down-regulated expression after ICSI. (A) Detection of CCNB1, c-MOS, ITPR1, and RYR3 proteins in ovulated quail eggs. (B) Western blot analysis 3 h after ICSI with injection of PLCZ1, CS, and ACO2 cRNA or the solvent alone. (C) Western blot analysis 3 h after ICSI with PLCZ1 cRNA alone, CS and ACO2 cRNAs, or the solvent alone. (D) Quantification of immunoreactivity after ICSI. Band intensities were quantified and expressed as the means ± standard deviations of three samples. Values with different letters are significantly different (P < 0.01).
    Ryr3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ryr3/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ryr3 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Egg Development After In Vitro Insemination in Japanese Quail ( Coturnix japonica )"

    Article Title: Egg Development After In Vitro Insemination in Japanese Quail ( Coturnix japonica )

    Journal: The Journal of Poultry Science

    doi: 10.2141/jpsa.2023001

    Presence of CCNB1, c-MOS, ITPR1, and RYR3 proteins in quail eggs and their down-regulated expression after ICSI. (A) Detection of CCNB1, c-MOS, ITPR1, and RYR3 proteins in ovulated quail eggs. (B) Western blot analysis 3 h after ICSI with injection of PLCZ1, CS, and ACO2 cRNA or the solvent alone. (C) Western blot analysis 3 h after ICSI with PLCZ1 cRNA alone, CS and ACO2 cRNAs, or the solvent alone. (D) Quantification of immunoreactivity after ICSI. Band intensities were quantified and expressed as the means ± standard deviations of three samples. Values with different letters are significantly different (P < 0.01).
    Figure Legend Snippet: Presence of CCNB1, c-MOS, ITPR1, and RYR3 proteins in quail eggs and their down-regulated expression after ICSI. (A) Detection of CCNB1, c-MOS, ITPR1, and RYR3 proteins in ovulated quail eggs. (B) Western blot analysis 3 h after ICSI with injection of PLCZ1, CS, and ACO2 cRNA or the solvent alone. (C) Western blot analysis 3 h after ICSI with PLCZ1 cRNA alone, CS and ACO2 cRNAs, or the solvent alone. (D) Quantification of immunoreactivity after ICSI. Band intensities were quantified and expressed as the means ± standard deviations of three samples. Values with different letters are significantly different (P < 0.01).

    Techniques Used: Expressing, Western Blot, Injection

    Changes in ITPR1, RYR3, CCNB1, and c-MOS protein expression levels in quail eggs following in vitro insemination with different sperm numbers. (A–C) Western blot analysis 3 h after in vitro insemination with 2 × 10 2 (A), 2 × 10 3 (B), or 2 × 10 4 (C) sperm, respectively. Numbers 1–6 represent each of the six eggs treated with sperm. Egg extracts after ICSI or PBS treatment were used as the control. (D) Quantification of immunoreactivity after in vitro insemination. Band intensities were quantified and are expressed as means ± standard deviations. The sample number of PBS- and ICSI-treated eggs is three each. Band intensities in these three samples were measured in each membrane.
    Figure Legend Snippet: Changes in ITPR1, RYR3, CCNB1, and c-MOS protein expression levels in quail eggs following in vitro insemination with different sperm numbers. (A–C) Western blot analysis 3 h after in vitro insemination with 2 × 10 2 (A), 2 × 10 3 (B), or 2 × 10 4 (C) sperm, respectively. Numbers 1–6 represent each of the six eggs treated with sperm. Egg extracts after ICSI or PBS treatment were used as the control. (D) Quantification of immunoreactivity after in vitro insemination. Band intensities were quantified and are expressed as means ± standard deviations. The sample number of PBS- and ICSI-treated eggs is three each. Band intensities in these three samples were measured in each membrane.

    Techniques Used: Expressing, In Vitro, Western Blot

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    Alomone Labs anti ryanodine receptor3 antibody ryr3
    Anti Ryanodine Receptor3 Antibody Ryr3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs ryr2
    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and <t>RyR2</t> activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.
    Ryr2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti ryr2 antibody
    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and <t>RyR2</t> activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.
    Anti Ryr2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs phospho ryr2 s2808 badrilla
    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and <t>RyR2</t> activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.
    Phospho Ryr2 S2808 Badrilla, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs rabbit anti ryanodine receptor 2 ryr2
    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and <t>RyR2</t> activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.
    Rabbit Anti Ryanodine Receptor 2 Ryr2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti ryr2
    Antibodies used in this study
    Anti Ryr2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs ryr3
    Presence of CCNB1, c-MOS, ITPR1, and <t>RYR3</t> proteins in quail eggs and their down-regulated expression after ICSI. (A) Detection of CCNB1, c-MOS, ITPR1, and RYR3 proteins in ovulated quail eggs. (B) Western blot analysis 3 h after ICSI with injection of PLCZ1, CS, and ACO2 cRNA or the solvent alone. (C) Western blot analysis 3 h after ICSI with PLCZ1 cRNA alone, CS and ACO2 cRNAs, or the solvent alone. (D) Quantification of immunoreactivity after ICSI. Band intensities were quantified and expressed as the means ± standard deviations of three samples. Values with different letters are significantly different (P < 0.01).
    Ryr3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and RyR2 activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.

    Journal: Molecular Metabolism

    Article Title: Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor

    doi: 10.1016/j.molmet.2024.101914

    Figure Lengend Snippet: Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and RyR2 activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.

    Article Snippet: Overnight immunoprecipitation of RyR2 with anti-RyR2 antibody (Alomone; catalog #ARR-002) was performed using a Pierce Co-IP Kit (Thermo Scientific).

    Techniques: Activity Assay, Western Blot

    Maternal exercise protects against Ryr2 oxidation and gene methylation in embryos . A) Western blot of Ryr2 and DNP (oxidation levels) and B) quantification in embryonic day 18.5 offspring hearts. C) Methylation status of RyR2. Expression levels of the methylated RyR2 promoter normalized by the expression of unmethylated RyR2 promoter in embryonic day 18.5 and perinatal day 0 offspring. D) Schematic figure identifying a pathway for maternal exercise to blunt the effect of a maternal high-fat diet (HFD) on changes in RYR methylation and mitochondrial dysfunction leading to RyR2 oxidation in cardiomyocytes. Data presented as mean ± SEM. Embryonic day 18.5 offspring from HS n = 3, HW n = 3 for RyR2 oxidation. e18.5 offspring and perinatal day 0 offspring (PND0) from CS n = 8, HS n = 5, HW n = 11 for RyR2 methylation. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗∗ P < 0.01 vs . Offspring of CS; ## P < 0.01, ### P < 0.001 vs . offspring of HS.

    Journal: Molecular Metabolism

    Article Title: Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor

    doi: 10.1016/j.molmet.2024.101914

    Figure Lengend Snippet: Maternal exercise protects against Ryr2 oxidation and gene methylation in embryos . A) Western blot of Ryr2 and DNP (oxidation levels) and B) quantification in embryonic day 18.5 offspring hearts. C) Methylation status of RyR2. Expression levels of the methylated RyR2 promoter normalized by the expression of unmethylated RyR2 promoter in embryonic day 18.5 and perinatal day 0 offspring. D) Schematic figure identifying a pathway for maternal exercise to blunt the effect of a maternal high-fat diet (HFD) on changes in RYR methylation and mitochondrial dysfunction leading to RyR2 oxidation in cardiomyocytes. Data presented as mean ± SEM. Embryonic day 18.5 offspring from HS n = 3, HW n = 3 for RyR2 oxidation. e18.5 offspring and perinatal day 0 offspring (PND0) from CS n = 8, HS n = 5, HW n = 11 for RyR2 methylation. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗∗ P < 0.01 vs . Offspring of CS; ## P < 0.01, ### P < 0.001 vs . offspring of HS.

    Article Snippet: Overnight immunoprecipitation of RyR2 with anti-RyR2 antibody (Alomone; catalog #ARR-002) was performed using a Pierce Co-IP Kit (Thermo Scientific).

    Techniques: Methylation, Western Blot, Expressing

    Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and RyR2 activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.

    Journal: Molecular Metabolism

    Article Title: Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor

    doi: 10.1016/j.molmet.2024.101914

    Figure Lengend Snippet: Maternal exercise prevents maternal-HFD induced increase in Ca 2+ sparks and RyR2 activity . (A) Representative images and (B) quantification of aberrant Ca 2+ sparks in permeabilized cardiomyocytes from female offspring from CS, HS, and HW dams, and with mitoTEMPO treatment (MT; 20 μM). (C) Western blot and (D) quantification of DNP (oxidation levels) and RyR2, both at 250 kDa. Data presented as mean ± SEM. Female offspring from CS n = 6 mice (3–11 cells per mouse for Ca 2+ sparks; 1–2 cells per mouse for caffeine amplitude), HS n = 3 mice (7–12 cells per mouse for Ca 2+ sparks; 2 cells per mouse for caffeine amplitude), HW n = 3 mice (6–11 cells per mouse for Ca 2+ sparks; 1–5 cells per mouse for caffeine amplitude). Female offspring from CS n = 5 mice, HS n = 6 mice, HW n = 8 mice for DNP oxidation WB. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗ P < 0.05; ∗∗∗ P < 0.001 vs . Offspring of CS; ## P < 0.01 vs . offspring of HS.

    Article Snippet: Overnight immunoprecipitation of RyR2 with anti-RyR2 antibody (Alomone; catalog #ARR-002) was performed using a Pierce Co-IP Kit (Thermo Scientific).

    Techniques: Activity Assay, Western Blot

    Maternal exercise protects against Ryr2 oxidation and gene methylation in embryos . A) Western blot of Ryr2 and DNP (oxidation levels) and B) quantification in embryonic day 18.5 offspring hearts. C) Methylation status of RyR2. Expression levels of the methylated RyR2 promoter normalized by the expression of unmethylated RyR2 promoter in embryonic day 18.5 and perinatal day 0 offspring. D) Schematic figure identifying a pathway for maternal exercise to blunt the effect of a maternal high-fat diet (HFD) on changes in RYR methylation and mitochondrial dysfunction leading to RyR2 oxidation in cardiomyocytes. Data presented as mean ± SEM. Embryonic day 18.5 offspring from HS n = 3, HW n = 3 for RyR2 oxidation. e18.5 offspring and perinatal day 0 offspring (PND0) from CS n = 8, HS n = 5, HW n = 11 for RyR2 methylation. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗∗ P < 0.01 vs . Offspring of CS; ## P < 0.01, ### P < 0.001 vs . offspring of HS.

    Journal: Molecular Metabolism

    Article Title: Maternal exercise preserves offspring cardiovascular health via oxidative regulation of the ryanodine receptor

    doi: 10.1016/j.molmet.2024.101914

    Figure Lengend Snippet: Maternal exercise protects against Ryr2 oxidation and gene methylation in embryos . A) Western blot of Ryr2 and DNP (oxidation levels) and B) quantification in embryonic day 18.5 offspring hearts. C) Methylation status of RyR2. Expression levels of the methylated RyR2 promoter normalized by the expression of unmethylated RyR2 promoter in embryonic day 18.5 and perinatal day 0 offspring. D) Schematic figure identifying a pathway for maternal exercise to blunt the effect of a maternal high-fat diet (HFD) on changes in RYR methylation and mitochondrial dysfunction leading to RyR2 oxidation in cardiomyocytes. Data presented as mean ± SEM. Embryonic day 18.5 offspring from HS n = 3, HW n = 3 for RyR2 oxidation. e18.5 offspring and perinatal day 0 offspring (PND0) from CS n = 8, HS n = 5, HW n = 11 for RyR2 methylation. One-way ANOVA with Tukey's multiple comparisons test were used for statistical analysis ∗∗ P < 0.01 vs . Offspring of CS; ## P < 0.01, ### P < 0.001 vs . offspring of HS.

    Article Snippet: Overnight immunoprecipitation of RyR2 with anti-RyR2 antibody (Alomone; catalog #ARR-002) was performed using a Pierce Co-IP Kit (Thermo Scientific).

    Techniques: Methylation, Western Blot, Expressing

    Antibodies used in this study

    Journal: Basic Research in Cardiology

    Article Title: Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes

    doi: 10.1007/s00395-023-00988-1

    Figure Lengend Snippet: Antibodies used in this study

    Article Snippet: Anti-RyR2 , Rat and Human , Alomone , Cat# ARR-002.

    Techniques: Western Blot

    COX7RP overexpression reduces spontaneous SR Ca 2+ release and mito-ROS in male ventricular myocytes under β-adrenergic stimulation. Conversely, COX7RP knock-down increases Ca 2+ waves and mito-ROS in females. A , B Representative Ca 2+ traces ( A ) and pooled data ( B ) for spontaneous Ca 2+ waves (SCW) latency in male (M), female (F), male overexpressing COX7RP (M + COX), and female expressing shRNA COX7RP (F + shC) ventricular cardiomyocytes (VCMs) exposed to 50 nmol/L isoproterenol (ISO, 5 min) after cessation of field stimulation (1 Hz). Mean ± SEM, n = 58–64, N = 6–9 preparations, * p < 0.05, p values were calculated using two-level random intercept model. C Pooled data for normalized fluorescence of mitochondria matrix ROS biosensor MLS-HyPer-7. Mean ± SEM, n = 29–42 VCMs, N = 6–9 animals, * p < 0.05, p values were calculated using two-level random intercept model. D Adenovirus–mediated expression of COX7RP in males and shRNA COX7RP in females alter RyR2 oxidation levels in rat VMs exposed to isoproterenol (ISO, 50 nmol/L) paced at 1 Hz for 5 min cultured for 48 h after infection (10 MOI). Immunoprecipitated RyR2s from M and F cultured VCM samples were probed with Anti-DNP antibody to detect amount of oxidized cysteines. E Pooled DNP optical density normalized to corresponding RyR2 signal. Mean ± SEM, N = 3 M, N = 4 F. * p < 0.05, Student’s t -test

    Journal: Basic Research in Cardiology

    Article Title: Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes

    doi: 10.1007/s00395-023-00988-1

    Figure Lengend Snippet: COX7RP overexpression reduces spontaneous SR Ca 2+ release and mito-ROS in male ventricular myocytes under β-adrenergic stimulation. Conversely, COX7RP knock-down increases Ca 2+ waves and mito-ROS in females. A , B Representative Ca 2+ traces ( A ) and pooled data ( B ) for spontaneous Ca 2+ waves (SCW) latency in male (M), female (F), male overexpressing COX7RP (M + COX), and female expressing shRNA COX7RP (F + shC) ventricular cardiomyocytes (VCMs) exposed to 50 nmol/L isoproterenol (ISO, 5 min) after cessation of field stimulation (1 Hz). Mean ± SEM, n = 58–64, N = 6–9 preparations, * p < 0.05, p values were calculated using two-level random intercept model. C Pooled data for normalized fluorescence of mitochondria matrix ROS biosensor MLS-HyPer-7. Mean ± SEM, n = 29–42 VCMs, N = 6–9 animals, * p < 0.05, p values were calculated using two-level random intercept model. D Adenovirus–mediated expression of COX7RP in males and shRNA COX7RP in females alter RyR2 oxidation levels in rat VMs exposed to isoproterenol (ISO, 50 nmol/L) paced at 1 Hz for 5 min cultured for 48 h after infection (10 MOI). Immunoprecipitated RyR2s from M and F cultured VCM samples were probed with Anti-DNP antibody to detect amount of oxidized cysteines. E Pooled DNP optical density normalized to corresponding RyR2 signal. Mean ± SEM, N = 3 M, N = 4 F. * p < 0.05, Student’s t -test

    Article Snippet: Anti-RyR2 , Rat and Human , Alomone , Cat# ARR-002.

    Techniques: Over Expression, Expressing, shRNA, Fluorescence, Cell Culture, Infection, Immunoprecipitation

    Presence of CCNB1, c-MOS, ITPR1, and RYR3 proteins in quail eggs and their down-regulated expression after ICSI. (A) Detection of CCNB1, c-MOS, ITPR1, and RYR3 proteins in ovulated quail eggs. (B) Western blot analysis 3 h after ICSI with injection of PLCZ1, CS, and ACO2 cRNA or the solvent alone. (C) Western blot analysis 3 h after ICSI with PLCZ1 cRNA alone, CS and ACO2 cRNAs, or the solvent alone. (D) Quantification of immunoreactivity after ICSI. Band intensities were quantified and expressed as the means ± standard deviations of three samples. Values with different letters are significantly different (P < 0.01).

    Journal: The Journal of Poultry Science

    Article Title: Egg Development After In Vitro Insemination in Japanese Quail ( Coturnix japonica )

    doi: 10.2141/jpsa.2023001

    Figure Lengend Snippet: Presence of CCNB1, c-MOS, ITPR1, and RYR3 proteins in quail eggs and their down-regulated expression after ICSI. (A) Detection of CCNB1, c-MOS, ITPR1, and RYR3 proteins in ovulated quail eggs. (B) Western blot analysis 3 h after ICSI with injection of PLCZ1, CS, and ACO2 cRNA or the solvent alone. (C) Western blot analysis 3 h after ICSI with PLCZ1 cRNA alone, CS and ACO2 cRNAs, or the solvent alone. (D) Quantification of immunoreactivity after ICSI. Band intensities were quantified and expressed as the means ± standard deviations of three samples. Values with different letters are significantly different (P < 0.01).

    Article Snippet: Rabbit anti-rat ITPR1 antibody (Alomone Labs Ltd., Jerusalem, Israel) and mouse anti-chicken RYR antibody (GeneTex, Inc., Irvine, CA, USA) were used to detect ITPR1 and RYR3, respectively.

    Techniques: Expressing, Western Blot, Injection

    Changes in ITPR1, RYR3, CCNB1, and c-MOS protein expression levels in quail eggs following in vitro insemination with different sperm numbers. (A–C) Western blot analysis 3 h after in vitro insemination with 2 × 10 2 (A), 2 × 10 3 (B), or 2 × 10 4 (C) sperm, respectively. Numbers 1–6 represent each of the six eggs treated with sperm. Egg extracts after ICSI or PBS treatment were used as the control. (D) Quantification of immunoreactivity after in vitro insemination. Band intensities were quantified and are expressed as means ± standard deviations. The sample number of PBS- and ICSI-treated eggs is three each. Band intensities in these three samples were measured in each membrane.

    Journal: The Journal of Poultry Science

    Article Title: Egg Development After In Vitro Insemination in Japanese Quail ( Coturnix japonica )

    doi: 10.2141/jpsa.2023001

    Figure Lengend Snippet: Changes in ITPR1, RYR3, CCNB1, and c-MOS protein expression levels in quail eggs following in vitro insemination with different sperm numbers. (A–C) Western blot analysis 3 h after in vitro insemination with 2 × 10 2 (A), 2 × 10 3 (B), or 2 × 10 4 (C) sperm, respectively. Numbers 1–6 represent each of the six eggs treated with sperm. Egg extracts after ICSI or PBS treatment were used as the control. (D) Quantification of immunoreactivity after in vitro insemination. Band intensities were quantified and are expressed as means ± standard deviations. The sample number of PBS- and ICSI-treated eggs is three each. Band intensities in these three samples were measured in each membrane.

    Article Snippet: Rabbit anti-rat ITPR1 antibody (Alomone Labs Ltd., Jerusalem, Israel) and mouse anti-chicken RYR antibody (GeneTex, Inc., Irvine, CA, USA) were used to detect ITPR1 and RYR3, respectively.

    Techniques: Expressing, In Vitro, Western Blot