rtcb reaction buffer  (New England Biolabs)


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    Name:
    Q5 Reaction Buffer Pack
    Description:
    Q5 Reaction Buffer Pack 6 0 ml
    Catalog Number:
    B9027S
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    28
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    6 0 ml
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    New England Biolabs rtcb reaction buffer
    Q5 Reaction Buffer Pack
    Q5 Reaction Buffer Pack 6 0 ml
    https://www.bioz.com/result/rtcb reaction buffer/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rtcb reaction buffer - by Bioz Stars, 2021-09
    99/100 stars

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    1) Product Images from "Ligation of 2′, 3′‐cyclic phosphate RNAs for the identification of microRNA binding sites"

    Article Title: Ligation of 2′, 3′‐cyclic phosphate RNAs for the identification of microRNA binding sites

    Journal: Febs Letters

    doi: 10.1002/1873-3468.13976

    RtcB‐mediated ligation of 2′, 3′‐cyclic phosphate miR‐34a‐5p strands to 5′‐OH mRNA mimics. (A) RtcB‐mediated ligation of miR‐34a‐5p > p (22 nt) to different LMTK3 mRNA mimics (64–72 nt) with 8 consecutive and (B) 14 consecutive complementary nt and a variable 5′ overhang of the LMTK3 counter‐strands (0, 2 or 8 nt overhang). Reaction mixtures contained 0.5 μ m RNA duplexes, 0.75 μ m RtcB, 0.1 m m GTP, 1 m m MnCl 2 , and 1x RtcB reaction buffer. Reference oligonucleotides for the respective ligation products (Controls) were chemically synthesized. Staining with SYBR Gold Nucleic Acid Gel Stain, n = 3, representative gels shown. Full gels shown in Fig. S6 .
    Figure Legend Snippet: RtcB‐mediated ligation of 2′, 3′‐cyclic phosphate miR‐34a‐5p strands to 5′‐OH mRNA mimics. (A) RtcB‐mediated ligation of miR‐34a‐5p > p (22 nt) to different LMTK3 mRNA mimics (64–72 nt) with 8 consecutive and (B) 14 consecutive complementary nt and a variable 5′ overhang of the LMTK3 counter‐strands (0, 2 or 8 nt overhang). Reaction mixtures contained 0.5 μ m RNA duplexes, 0.75 μ m RtcB, 0.1 m m GTP, 1 m m MnCl 2 , and 1x RtcB reaction buffer. Reference oligonucleotides for the respective ligation products (Controls) were chemically synthesized. Staining with SYBR Gold Nucleic Acid Gel Stain, n = 3, representative gels shown. Full gels shown in Fig. S6 .

    Techniques Used: Ligation, Synthesized, Staining

    Mechanistic details of RtcB‐mediated RNA > p ligation (A) Different mechanisms suggested for bacterial and human RNA > p ligation. Direct ligation between RNA 5′‐OH and 2′, 3′‐cyclic phosphate termini has been suggested in HeLa lysate [ 19 , 37 , 38 ]. For bacterial RtcB, hydrolysis of the 2′, 3′‐cycP to a 3′‐P terminus and subsequent ligation of the 3′‐P terminus has been suggested [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. (B) RtcB‐mediated cyclization of the stem‐loop RNApre‐20 > pin H 2 16 O and (C) H 2 18 O. RtcB ligation was performed at 37 °C for 1 h in the presence of 50 m m Tris/HCl (pH = 8), 1 m m MnCl 2 , 0.1 m m GTP, 0.75 µ m RtcB and 0.5 µ m pre‐20 > p. Ligation was quenched through the addition of Na 2 EDTA to a final concentration of 50 m m . LC‐MS analysis was performed on an Agilent 1200/6130 system fitted with a Waters acquity UPLC OST C‐18 column (2.1 × 50 mm, 1.7 μm) at 65 °C, with a gradient of 5–35% eluent B in 14 min with a flow rate of 0.3 mL·min −1 . Eluent A was aqueous hexafluoroisopropanol (0.4 M) containing triethylamine (15 m m ). Eluent B was methanol. UV trace (260 nm) between 7.5 and 10 min is shown. B = nucleobase.
    Figure Legend Snippet: Mechanistic details of RtcB‐mediated RNA > p ligation (A) Different mechanisms suggested for bacterial and human RNA > p ligation. Direct ligation between RNA 5′‐OH and 2′, 3′‐cyclic phosphate termini has been suggested in HeLa lysate [ 19 , 37 , 38 ]. For bacterial RtcB, hydrolysis of the 2′, 3′‐cycP to a 3′‐P terminus and subsequent ligation of the 3′‐P terminus has been suggested [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. (B) RtcB‐mediated cyclization of the stem‐loop RNApre‐20 > pin H 2 16 O and (C) H 2 18 O. RtcB ligation was performed at 37 °C for 1 h in the presence of 50 m m Tris/HCl (pH = 8), 1 m m MnCl 2 , 0.1 m m GTP, 0.75 µ m RtcB and 0.5 µ m pre‐20 > p. Ligation was quenched through the addition of Na 2 EDTA to a final concentration of 50 m m . LC‐MS analysis was performed on an Agilent 1200/6130 system fitted with a Waters acquity UPLC OST C‐18 column (2.1 × 50 mm, 1.7 μm) at 65 °C, with a gradient of 5–35% eluent B in 14 min with a flow rate of 0.3 mL·min −1 . Eluent A was aqueous hexafluoroisopropanol (0.4 M) containing triethylamine (15 m m ). Eluent B was methanol. UV trace (260 nm) between 7.5 and 10 min is shown. B = nucleobase.

    Techniques Used: Ligation, Concentration Assay, Liquid Chromatography with Mass Spectroscopy

    Cross‐linking strategies of miRNAs to their mRNA targets in CLIP protocols. (A) Ligation of miRNA‐mRNA target pairs in miRISC after controlled treatment of the target mRNA with RNase A or T1 during CLASH [ 15 ] or CLEAR‐CLIP [ 14 ] methods; chimeras are formed with low efficiency after 5′‐phosphorylation and T4‐ligase mediated end‐joining. RNA sequencing reveals the miRNA, its mRNA target, and the site of interaction. (B) MiR‐CLIP probes use psoralen to cross‐link to their target mRNAs. MiRNA‐mRNA target pairs are enriched by biotin–streptavidin enrichment [ 6 ]. (C) The proposed protocol (this study). MiRNA‐mRNA chimeras may form with high efficiency after RtcB‐mediated ligation of cyclic phosphate‐terminated miRNA probes to the 5′‐hydroxyl terminus of the target RNA. RNA sequencing reveals the miRNA, its mRNA target, and the site of interaction.
    Figure Legend Snippet: Cross‐linking strategies of miRNAs to their mRNA targets in CLIP protocols. (A) Ligation of miRNA‐mRNA target pairs in miRISC after controlled treatment of the target mRNA with RNase A or T1 during CLASH [ 15 ] or CLEAR‐CLIP [ 14 ] methods; chimeras are formed with low efficiency after 5′‐phosphorylation and T4‐ligase mediated end‐joining. RNA sequencing reveals the miRNA, its mRNA target, and the site of interaction. (B) MiR‐CLIP probes use psoralen to cross‐link to their target mRNAs. MiRNA‐mRNA target pairs are enriched by biotin–streptavidin enrichment [ 6 ]. (C) The proposed protocol (this study). MiRNA‐mRNA chimeras may form with high efficiency after RtcB‐mediated ligation of cyclic phosphate‐terminated miRNA probes to the 5′‐hydroxyl terminus of the target RNA. RNA sequencing reveals the miRNA, its mRNA target, and the site of interaction.

    Techniques Used: Cross-linking Immunoprecipitation, Ligation, RNA Sequencing Assay

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    Amplification:

    Article Title: CRISPR-Cas9 bends and twists DNA to read its sequence
    Article Snippet: .. Each reaction was 400 µL total (split into 4 x 100-µL aliquots) and contained 1X Q5 reaction buffer (New England BioLabs), 200 µM dNTPs, 200 nM forward amplification primer, 200 nM reverse amplification primer, 1 nM forward assembly primer, 1 nM reverse assembly primer, 0.02 U/µL Q5 polymerase. ..

    Article Title: The B.1.427/1.429 (epsilon) SARS-CoV-2 variants are more virulent than ancestral B.1 (614G) in Syrian hamsters
    Article Snippet: .. PCR amplification was performed using the QUILLS primer set and Q5® High-Fidelity DNA Polymerase (NEB #M0491) as follows: 15.4 µl of water, 5 µl of Q5 reaction buffer, 0.5 µl of dNTPS (NEB N0447S), 0.25 µl of enzyme and 1.35 µl of primer for each pool (pool 1 and pool 2). ..

    Article Title: An engineered IL-2 reprogrammed for anti-tumor therapy using a semi-synthetic organism
    Article Snippet: .. The IL-2 Golden Gate entry vectors were linearized by PCR amplification using 0.02 U/µL Q5 DNA Polymerase with 200 µM dNTPs, 0.5× SYBR Green, 1× Q5 Reaction buffer, 2 ng of template per 50 µL reaction and 0.5 µM of primers which amplify from the IL-2 Golden Gate entry site using the following conditions: [98 °C 0:30 | 20× (98 °C 0:10 | 60 °C 0:10 | 72 °C 3:00)]. ..

    Article Title: Differences in Gut Microbiome Composition Between Sympatric Wild and Allopatric Laboratory Populations of Omnivorous Cockroaches
    Article Snippet: .. The secondary amplification mixture contained 1 Q5 reaction buffer, 200 M dNTPs, 0.5 M 515F, 0.5 M 806R, 2 ng DNA, and 0.02 U/L Q5 polymerase. ..

    Polymerase Chain Reaction:

    Article Title: Metagenomes, metatranscriptomes and microbiomes of naturally decomposing deadwood
    Article Snippet: .. PCR premix for ITS2 or 16S rRNA gene metabarcoding contained 5 μl of 5× Q5 Reaction Buffer, 5 μl of 5× Q5 High GC Enhancer and 0.25 μl Q5 High-Fidelity DNA Polymerase (New England Biolabs), 1.5 μl of BSA (10 mg ml−1 ), 0.5 μl of dNTPs Nucleotide Mix 10 mM (Bioline), 1 μl of each primer, 9.75 μl of H2O and 1 μl of template DNA. ..

    Article Title: The B.1.427/1.429 (epsilon) SARS-CoV-2 variants are more virulent than ancestral B.1 (614G) in Syrian hamsters
    Article Snippet: .. PCR amplification was performed using the QUILLS primer set and Q5® High-Fidelity DNA Polymerase (NEB #M0491) as follows: 15.4 µl of water, 5 µl of Q5 reaction buffer, 0.5 µl of dNTPS (NEB N0447S), 0.25 µl of enzyme and 1.35 µl of primer for each pool (pool 1 and pool 2). ..

    Article Title: An engineered IL-2 reprogrammed for anti-tumor therapy using a semi-synthetic organism
    Article Snippet: .. The IL-2 Golden Gate entry vectors were linearized by PCR amplification using 0.02 U/µL Q5 DNA Polymerase with 200 µM dNTPs, 0.5× SYBR Green, 1× Q5 Reaction buffer, 2 ng of template per 50 µL reaction and 0.5 µM of primers which amplify from the IL-2 Golden Gate entry site using the following conditions: [98 °C 0:30 | 20× (98 °C 0:10 | 60 °C 0:10 | 72 °C 3:00)]. ..

    Article Title: Cappable-Seq Reveals Specific Patterns of Metabolism and Virulence for Salmonella Typhimurium Intracellular Survival within Acanthamoeba castellanii
    Article Snippet: .. Lysates were inactivated by heating at 95 °C for 5 min. PCR was conducted using a thermocycler (T100 Thermal Cycler, Bio-Rad, Hercules, CA, USA) in a 25 μL reaction mixture containing 3.75 μL of 5× Q5 reaction buffer (New England Biolabs, Ipswich, MA, USA), 1.25 μL of 2.5 mM dNTPs mix, 0.5 μL of each primer (10 mM), 0.2 μL of Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and 2 μL of template DNA. ..

    Article Title: Cross-neutralization of SARS-CoV-2 B.1.1.7 and P.1 variants in vaccinated, convalescent and P.1 infected
    Article Snippet: .. The PCR mixture included 3 μl cDNA, 5 µl 5X Q5 Reaction buffer (NEB), 4 pmol P1004-Fwd (5’-AATTAGAGAAAACAACAGAGT-3’) and P976-Rev (5’- AAATTTGTGGGTATGGCAATAGAGTTA-3’), 150 µM dNTPs and 0.5U Q5 Hot Start High-Fidelity DNA Polymerase (NEB) in a final volume of 25 μl. ..

    SYBR Green Assay:

    Article Title: An engineered IL-2 reprogrammed for anti-tumor therapy using a semi-synthetic organism
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  • 99
    New England Biolabs rtcb reaction buffer
    <t>RtcB‐mediated</t> ligation of 2′, 3′‐cyclic phosphate miR‐34a‐5p strands to 5′‐OH mRNA mimics. (A) RtcB‐mediated ligation of miR‐34a‐5p > p (22 nt) to different LMTK3 mRNA mimics (64–72 nt) with 8 consecutive and (B) 14 consecutive complementary nt and a variable 5′ overhang of the LMTK3 counter‐strands (0, 2 or 8 nt overhang). Reaction mixtures contained 0.5 μ m <t>RNA</t> duplexes, 0.75 μ m RtcB, 0.1 m m GTP, 1 m m MnCl 2 , and 1x RtcB reaction buffer. Reference oligonucleotides for the respective ligation products (Controls) were chemically synthesized. Staining with SYBR Gold Nucleic Acid Gel Stain, n = 3, representative gels shown. Full gels shown in Fig. S6 .
    Rtcb Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rtcb reaction buffer/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rtcb reaction buffer - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    95
    New England Biolabs 10x rtcb reaction buffer
    <t>RtcB‐mediated</t> ligation of 2′, 3′‐cyclic phosphate miR‐34a‐5p strands to 5′‐OH mRNA mimics. (A) RtcB‐mediated ligation of miR‐34a‐5p > p (22 nt) to different LMTK3 mRNA mimics (64–72 nt) with 8 consecutive and (B) 14 consecutive complementary nt and a variable 5′ overhang of the LMTK3 counter‐strands (0, 2 or 8 nt overhang). Reaction mixtures contained 0.5 μ m <t>RNA</t> duplexes, 0.75 μ m RtcB, 0.1 m m GTP, 1 m m MnCl 2 , and 1x RtcB reaction buffer. Reference oligonucleotides for the respective ligation products (Controls) were chemically synthesized. Staining with SYBR Gold Nucleic Acid Gel Stain, n = 3, representative gels shown. Full gels shown in Fig. S6 .
    10x Rtcb Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x rtcb reaction buffer/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10x rtcb reaction buffer - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    RtcB‐mediated ligation of 2′, 3′‐cyclic phosphate miR‐34a‐5p strands to 5′‐OH mRNA mimics. (A) RtcB‐mediated ligation of miR‐34a‐5p > p (22 nt) to different LMTK3 mRNA mimics (64–72 nt) with 8 consecutive and (B) 14 consecutive complementary nt and a variable 5′ overhang of the LMTK3 counter‐strands (0, 2 or 8 nt overhang). Reaction mixtures contained 0.5 μ m RNA duplexes, 0.75 μ m RtcB, 0.1 m m GTP, 1 m m MnCl 2 , and 1x RtcB reaction buffer. Reference oligonucleotides for the respective ligation products (Controls) were chemically synthesized. Staining with SYBR Gold Nucleic Acid Gel Stain, n = 3, representative gels shown. Full gels shown in Fig. S6 .

    Journal: Febs Letters

    Article Title: Ligation of 2′, 3′‐cyclic phosphate RNAs for the identification of microRNA binding sites

    doi: 10.1002/1873-3468.13976

    Figure Lengend Snippet: RtcB‐mediated ligation of 2′, 3′‐cyclic phosphate miR‐34a‐5p strands to 5′‐OH mRNA mimics. (A) RtcB‐mediated ligation of miR‐34a‐5p > p (22 nt) to different LMTK3 mRNA mimics (64–72 nt) with 8 consecutive and (B) 14 consecutive complementary nt and a variable 5′ overhang of the LMTK3 counter‐strands (0, 2 or 8 nt overhang). Reaction mixtures contained 0.5 μ m RNA duplexes, 0.75 μ m RtcB, 0.1 m m GTP, 1 m m MnCl 2 , and 1x RtcB reaction buffer. Reference oligonucleotides for the respective ligation products (Controls) were chemically synthesized. Staining with SYBR Gold Nucleic Acid Gel Stain, n = 3, representative gels shown. Full gels shown in Fig. S6 .

    Article Snippet: RtcB ligation assays One microlitre 10 μm annealed RNA duplexes (0.5 μm final) were mixed with 2 μL 10× RtcB reaction buffer (New England Biolabs), 1 μL 15 μm RtcB (0.75 μm final; New England Biolabs, M0458S), 2 μL 1 mm GTP, 2 μL 10 mm MnCl2 and 12 μL RNase‐free H2O (total volume = 20 μL) and incubated at 37 °C for 1 h. Samples were analyzed by 15% dPAGE and visualized with SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher).

    Techniques: Ligation, Synthesized, Staining

    Mechanistic details of RtcB‐mediated RNA > p ligation (A) Different mechanisms suggested for bacterial and human RNA > p ligation. Direct ligation between RNA 5′‐OH and 2′, 3′‐cyclic phosphate termini has been suggested in HeLa lysate [ 19 , 37 , 38 ]. For bacterial RtcB, hydrolysis of the 2′, 3′‐cycP to a 3′‐P terminus and subsequent ligation of the 3′‐P terminus has been suggested [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. (B) RtcB‐mediated cyclization of the stem‐loop RNApre‐20 > pin H 2 16 O and (C) H 2 18 O. RtcB ligation was performed at 37 °C for 1 h in the presence of 50 m m Tris/HCl (pH = 8), 1 m m MnCl 2 , 0.1 m m GTP, 0.75 µ m RtcB and 0.5 µ m pre‐20 > p. Ligation was quenched through the addition of Na 2 EDTA to a final concentration of 50 m m . LC‐MS analysis was performed on an Agilent 1200/6130 system fitted with a Waters acquity UPLC OST C‐18 column (2.1 × 50 mm, 1.7 μm) at 65 °C, with a gradient of 5–35% eluent B in 14 min with a flow rate of 0.3 mL·min −1 . Eluent A was aqueous hexafluoroisopropanol (0.4 M) containing triethylamine (15 m m ). Eluent B was methanol. UV trace (260 nm) between 7.5 and 10 min is shown. B = nucleobase.

    Journal: Febs Letters

    Article Title: Ligation of 2′, 3′‐cyclic phosphate RNAs for the identification of microRNA binding sites

    doi: 10.1002/1873-3468.13976

    Figure Lengend Snippet: Mechanistic details of RtcB‐mediated RNA > p ligation (A) Different mechanisms suggested for bacterial and human RNA > p ligation. Direct ligation between RNA 5′‐OH and 2′, 3′‐cyclic phosphate termini has been suggested in HeLa lysate [ 19 , 37 , 38 ]. For bacterial RtcB, hydrolysis of the 2′, 3′‐cycP to a 3′‐P terminus and subsequent ligation of the 3′‐P terminus has been suggested [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. (B) RtcB‐mediated cyclization of the stem‐loop RNApre‐20 > pin H 2 16 O and (C) H 2 18 O. RtcB ligation was performed at 37 °C for 1 h in the presence of 50 m m Tris/HCl (pH = 8), 1 m m MnCl 2 , 0.1 m m GTP, 0.75 µ m RtcB and 0.5 µ m pre‐20 > p. Ligation was quenched through the addition of Na 2 EDTA to a final concentration of 50 m m . LC‐MS analysis was performed on an Agilent 1200/6130 system fitted with a Waters acquity UPLC OST C‐18 column (2.1 × 50 mm, 1.7 μm) at 65 °C, with a gradient of 5–35% eluent B in 14 min with a flow rate of 0.3 mL·min −1 . Eluent A was aqueous hexafluoroisopropanol (0.4 M) containing triethylamine (15 m m ). Eluent B was methanol. UV trace (260 nm) between 7.5 and 10 min is shown. B = nucleobase.

    Article Snippet: RtcB ligation assays One microlitre 10 μm annealed RNA duplexes (0.5 μm final) were mixed with 2 μL 10× RtcB reaction buffer (New England Biolabs), 1 μL 15 μm RtcB (0.75 μm final; New England Biolabs, M0458S), 2 μL 1 mm GTP, 2 μL 10 mm MnCl2 and 12 μL RNase‐free H2O (total volume = 20 μL) and incubated at 37 °C for 1 h. Samples were analyzed by 15% dPAGE and visualized with SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher).

    Techniques: Ligation, Concentration Assay, Liquid Chromatography with Mass Spectroscopy

    Cross‐linking strategies of miRNAs to their mRNA targets in CLIP protocols. (A) Ligation of miRNA‐mRNA target pairs in miRISC after controlled treatment of the target mRNA with RNase A or T1 during CLASH [ 15 ] or CLEAR‐CLIP [ 14 ] methods; chimeras are formed with low efficiency after 5′‐phosphorylation and T4‐ligase mediated end‐joining. RNA sequencing reveals the miRNA, its mRNA target, and the site of interaction. (B) MiR‐CLIP probes use psoralen to cross‐link to their target mRNAs. MiRNA‐mRNA target pairs are enriched by biotin–streptavidin enrichment [ 6 ]. (C) The proposed protocol (this study). MiRNA‐mRNA chimeras may form with high efficiency after RtcB‐mediated ligation of cyclic phosphate‐terminated miRNA probes to the 5′‐hydroxyl terminus of the target RNA. RNA sequencing reveals the miRNA, its mRNA target, and the site of interaction.

    Journal: Febs Letters

    Article Title: Ligation of 2′, 3′‐cyclic phosphate RNAs for the identification of microRNA binding sites

    doi: 10.1002/1873-3468.13976

    Figure Lengend Snippet: Cross‐linking strategies of miRNAs to their mRNA targets in CLIP protocols. (A) Ligation of miRNA‐mRNA target pairs in miRISC after controlled treatment of the target mRNA with RNase A or T1 during CLASH [ 15 ] or CLEAR‐CLIP [ 14 ] methods; chimeras are formed with low efficiency after 5′‐phosphorylation and T4‐ligase mediated end‐joining. RNA sequencing reveals the miRNA, its mRNA target, and the site of interaction. (B) MiR‐CLIP probes use psoralen to cross‐link to their target mRNAs. MiRNA‐mRNA target pairs are enriched by biotin–streptavidin enrichment [ 6 ]. (C) The proposed protocol (this study). MiRNA‐mRNA chimeras may form with high efficiency after RtcB‐mediated ligation of cyclic phosphate‐terminated miRNA probes to the 5′‐hydroxyl terminus of the target RNA. RNA sequencing reveals the miRNA, its mRNA target, and the site of interaction.

    Article Snippet: RtcB ligation assays One microlitre 10 μm annealed RNA duplexes (0.5 μm final) were mixed with 2 μL 10× RtcB reaction buffer (New England Biolabs), 1 μL 15 μm RtcB (0.75 μm final; New England Biolabs, M0458S), 2 μL 1 mm GTP, 2 μL 10 mm MnCl2 and 12 μL RNase‐free H2O (total volume = 20 μL) and incubated at 37 °C for 1 h. Samples were analyzed by 15% dPAGE and visualized with SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher).

    Techniques: Cross-linking Immunoprecipitation, Ligation, RNA Sequencing Assay