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rt112 cell line  (DSMZ)


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    Structured Review

    DSMZ rt112 cell line
    A. Nectin-4 expression on three cell lines in comparison with SUM190PT. B. MCF7, HT29, <t>RT112</t> and SUM190PT cell lines were treated with CD30-MMAE(negative control) and enfortumab vedotin. MCF7, HT29 and RT112 cell lines are resistant to enfortumab vedotin whereas SUM190PT is sensible. n = 2 biologic replicates were examined in one experiment. C . 4-hour caspase 3/7 cytotoxicity assay indicating a tumor killing of CARTN4 cells against MCF7, HT29, RT112. n=4 healthy donors were examined in three independent experiments. Data in panels B, and C are presented as Mean +/- SEM. P-values were calculated using a 2way ANOVA. Source data are provided in the Source Data file.
    Rt112 Cell Line, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rt112+cells/RT-112/bio_rxiv__2025__09__30__679440-176-0-6
    Average 95 stars, based on 156 article reviews
    rt112 cell line - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "CAR T cells targeting Nectin-4 safely overcome resistance to anti-Nectin-4 antibody-drug conjugate in solid tumors"

    Article Title: CAR T cells targeting Nectin-4 safely overcome resistance to anti-Nectin-4 antibody-drug conjugate in solid tumors

    Journal: bioRxiv

    doi: 10.1101/2025.09.30.679440

    A. Nectin-4 expression on three cell lines in comparison with SUM190PT. B. MCF7, HT29, RT112 and SUM190PT cell lines were treated with CD30-MMAE(negative control) and enfortumab vedotin. MCF7, HT29 and RT112 cell lines are resistant to enfortumab vedotin whereas SUM190PT is sensible. n = 2 biologic replicates were examined in one experiment. C . 4-hour caspase 3/7 cytotoxicity assay indicating a tumor killing of CARTN4 cells against MCF7, HT29, RT112. n=4 healthy donors were examined in three independent experiments. Data in panels B, and C are presented as Mean +/- SEM. P-values were calculated using a 2way ANOVA. Source data are provided in the Source Data file.
    Figure Legend Snippet: A. Nectin-4 expression on three cell lines in comparison with SUM190PT. B. MCF7, HT29, RT112 and SUM190PT cell lines were treated with CD30-MMAE(negative control) and enfortumab vedotin. MCF7, HT29 and RT112 cell lines are resistant to enfortumab vedotin whereas SUM190PT is sensible. n = 2 biologic replicates were examined in one experiment. C . 4-hour caspase 3/7 cytotoxicity assay indicating a tumor killing of CARTN4 cells against MCF7, HT29, RT112. n=4 healthy donors were examined in three independent experiments. Data in panels B, and C are presented as Mean +/- SEM. P-values were calculated using a 2way ANOVA. Source data are provided in the Source Data file.

    Techniques Used: Expressing, Comparison, Negative Control, Cytotoxicity Assay

    Related Articles

    Cell Culture:

    Article Title: The mycotoxin viriditoxin induces leukemia- and lymphoma-specific apoptosis by targeting mitochondrial metabolism.
    Article Snippet: .. Cell Death and Disease (2022) 13:938 Cell lines, primary cells, and cell culture DG75 cells (human B cell Burkitt lymphoma; #ACC-83), HeLa cells (human cervix carcinoma; #ACC-57), HT29 cells (human colon carcinoma; #ACC-299), HCT116 cells (human colon carcinoma; #ACC-581), Jurkat (JM) cells (human T cell acute lymphoblastic leukemia; #ACC-282), MCF7 cells (human breast carcinoma; #ACC-115), RT112 cells (human urinary bladder carcinoma #ACC-418), SH-SY5Y cells (human neuroblastoma; #ACC-209), HL60 (human acute myeloid leukemia; #ACC-3), HPBALL (human T cell acute lymphoblastic leukemia; #ACC-483), MOLT4 (human T cell acute lymphoblastic leukemia; #ACC-362), K562 (human chronic myeloid leukemia; #ACC-10), and SUPB15 (human B cell acute lymphoblastic leukemia; #ACC-389) were obtained from DSMZ and 143B cells (human osteosarcoma; #CRL-8303) from ATCC. ..

    other:

    Article Title: The Secretome Engages STAT3 to Favor a Cytokine-rich Microenvironment in Mediating Acquired Resistance to FGFR Inhibitors.
    Article Snippet: 2 Acquired resistance severely hinders the application of small molecule inhibitors.. Our understanding of acquired resistance related to fibroblast growth factor receptors (FGFRs) is limited.. Here, to explore the underlying mechanism of acquired resistance in FGFR-aberrant cancer cells, we generated cells resistant to multiple FGFR inhibitors and investigated the potential mechanisms underlying acquired resistance.

    Article Title: Preclinical evaluation of 3D185, a novel potent inhibitor of FGFR1/2/3 and CSF-1R, in FGFR-dependent and macrophage-dominant cancer models
    Article Snippet: RT112, OPM2, CAL51, HCC366 and HCC78 cells were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

    Article Title: c-Myc Alteration Determines the Therapeutic Response to FGFR Inhibitors
    Article Snippet: c-Myc alteration determines the therapeutic response to FGFR inhibitors Hongyan Liu, Jing Ai, Aijun Shen, Yi Chen, Xinyi Wang, Xia Peng, Hui Chen, Yanyan Shen, Min Huang*, Jian Ding*, Meiyu Geng* Author affiliations: Division of Anti-tumor Pharmacology, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai 201203, P.R.. China.

    Knockdown:

    Article Title: The small conductance calcium-activated potassium channel 3 (SK3) is a molecular target for Edelfosine to reduce the invasive potential of urothelial carcinoma cells.
    Article Snippet: Metastasis is the survival-determining factor in urothelial carcinoma (UC) of the urinary bladder.. The small conductance calcium-activated potassium channel 3 (SK3) enhances tumor cell invasion in breast cancer and malignant melanoma.. Since Edelfosine, a glycerophospholipid with antitumoral properties, effectively inhibits SK3 channel activity, our goal was to evaluate SK3 as a potential molecular target to inhibit the gain of an invasive phenotype in UC.

    Migration:

    Article Title: The small conductance calcium-activated potassium channel 3 (SK3) is a molecular target for Edelfosine to reduce the invasive potential of urothelial carcinoma cells.
    Article Snippet: Metastasis is the survival-determining factor in urothelial carcinoma (UC) of the urinary bladder.. The small conductance calcium-activated potassium channel 3 (SK3) enhances tumor cell invasion in breast cancer and malignant melanoma.. Since Edelfosine, a glycerophospholipid with antitumoral properties, effectively inhibits SK3 channel activity, our goal was to evaluate SK3 as a potential molecular target to inhibit the gain of an invasive phenotype in UC.

    Control:

    Article Title: The small conductance calcium-activated potassium channel 3 (SK3) is a molecular target for Edelfosine to reduce the invasive potential of urothelial carcinoma cells.
    Article Snippet: Metastasis is the survival-determining factor in urothelial carcinoma (UC) of the urinary bladder.. The small conductance calcium-activated potassium channel 3 (SK3) enhances tumor cell invasion in breast cancer and malignant melanoma.. Since Edelfosine, a glycerophospholipid with antitumoral properties, effectively inhibits SK3 channel activity, our goal was to evaluate SK3 as a potential molecular target to inhibit the gain of an invasive phenotype in UC.

    Boyden Chamber Assay:

    Article Title: The small conductance calcium-activated potassium channel 3 (SK3) is a molecular target for Edelfosine to reduce the invasive potential of urothelial carcinoma cells.
    Article Snippet: Metastasis is the survival-determining factor in urothelial carcinoma (UC) of the urinary bladder.. The small conductance calcium-activated potassium channel 3 (SK3) enhances tumor cell invasion in breast cancer and malignant melanoma.. Since Edelfosine, a glycerophospholipid with antitumoral properties, effectively inhibits SK3 channel activity, our goal was to evaluate SK3 as a potential molecular target to inhibit the gain of an invasive phenotype in UC.



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    European Collection of Authenticated Cell Cultures rt112 cell line
    A. Nectin-4 expression on three cell lines in comparison with SUM190PT. B. MCF7, HT29, <t>RT112</t> and SUM190PT cell lines were treated with CD30-MMAE(negative control) and enfortumab vedotin. MCF7, HT29 and RT112 cell lines are resistant to enfortumab vedotin whereas SUM190PT is sensible. n = 2 biologic replicates were examined in one experiment. C . 4-hour caspase 3/7 cytotoxicity assay indicating a tumor killing of CARTN4 cells against MCF7, HT29, RT112. n=4 healthy donors were examined in three independent experiments. Data in panels B, and C are presented as Mean +/- SEM. P-values were calculated using a 2way ANOVA. Source data are provided in the Source Data file.
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    A. Nectin-4 expression on three cell lines in comparison with SUM190PT. B. MCF7, HT29, <t>RT112</t> and SUM190PT cell lines were treated with CD30-MMAE(negative control) and enfortumab vedotin. MCF7, HT29 and RT112 cell lines are resistant to enfortumab vedotin whereas SUM190PT is sensible. n = 2 biologic replicates were examined in one experiment. C . 4-hour caspase 3/7 cytotoxicity assay indicating a tumor killing of CARTN4 cells against MCF7, HT29, RT112. n=4 healthy donors were examined in three independent experiments. Data in panels B, and C are presented as Mean +/- SEM. P-values were calculated using a 2way ANOVA. Source data are provided in the Source Data file.
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    A. Nectin-4 expression on three cell lines in comparison with SUM190PT. B. MCF7, HT29, <t>RT112</t> and SUM190PT cell lines were treated with CD30-MMAE(negative control) and enfortumab vedotin. MCF7, HT29 and RT112 cell lines are resistant to enfortumab vedotin whereas SUM190PT is sensible. n = 2 biologic replicates were examined in one experiment. C . 4-hour caspase 3/7 cytotoxicity assay indicating a tumor killing of CARTN4 cells against MCF7, HT29, RT112. n=4 healthy donors were examined in three independent experiments. Data in panels B, and C are presented as Mean +/- SEM. P-values were calculated using a 2way ANOVA. Source data are provided in the Source Data file.
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    Image Search Results


    ( a ) Western blot analysis of EGFR, Nectin-4, and TROP-2 expression in the BC cell lines RT4 and RT112 and in the antigen-negative control cell line CHO. ( b ) Cell binding of the antibodies and conjugates to the target cells as demonstrated by flow cytometry. Binding of the isotype control antibody is shown in gray.

    Journal: Molecules

    Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

    doi: 10.3390/molecules30244802

    Figure Lengend Snippet: ( a ) Western blot analysis of EGFR, Nectin-4, and TROP-2 expression in the BC cell lines RT4 and RT112 and in the antigen-negative control cell line CHO. ( b ) Cell binding of the antibodies and conjugates to the target cells as demonstrated by flow cytometry. Binding of the isotype control antibody is shown in gray.

    Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

    Techniques: Western Blot, Expressing, Negative Control, Binding Assay, Flow Cytometry, Control

    Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2, and Sac T120C/D265C -WB692-CB2 after irradiation with different light doses in RT4 and RT112 cells. CHO cells served as antigen-negative controls. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

    Journal: Molecules

    Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

    doi: 10.3390/molecules30244802

    Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2, and Sac T120C/D265C -WB692-CB2 after irradiation with different light doses in RT4 and RT112 cells. CHO cells served as antigen-negative controls. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

    Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

    Techniques: Irradiation

    Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2 and Sac 120T/265C -WB692-CB2 alone and in combination after irradiation with a light dose of 64 J/cm 2 in RT4 and RT112 cells. Control samples were treated with the uncoupled antibodies or the free dye. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

    Journal: Molecules

    Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

    doi: 10.3390/molecules30244802

    Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2 and Sac 120T/265C -WB692-CB2 alone and in combination after irradiation with a light dose of 64 J/cm 2 in RT4 and RT112 cells. Control samples were treated with the uncoupled antibodies or the free dye. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

    Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

    Techniques: Irradiation, Control

    A. Nectin-4 expression on three cell lines in comparison with SUM190PT. B. MCF7, HT29, RT112 and SUM190PT cell lines were treated with CD30-MMAE(negative control) and enfortumab vedotin. MCF7, HT29 and RT112 cell lines are resistant to enfortumab vedotin whereas SUM190PT is sensible. n = 2 biologic replicates were examined in one experiment. C . 4-hour caspase 3/7 cytotoxicity assay indicating a tumor killing of CARTN4 cells against MCF7, HT29, RT112. n=4 healthy donors were examined in three independent experiments. Data in panels B, and C are presented as Mean +/- SEM. P-values were calculated using a 2way ANOVA. Source data are provided in the Source Data file.

    Journal: bioRxiv

    Article Title: CAR T cells targeting Nectin-4 safely overcome resistance to anti-Nectin-4 antibody-drug conjugate in solid tumors

    doi: 10.1101/2025.09.30.679440

    Figure Lengend Snippet: A. Nectin-4 expression on three cell lines in comparison with SUM190PT. B. MCF7, HT29, RT112 and SUM190PT cell lines were treated with CD30-MMAE(negative control) and enfortumab vedotin. MCF7, HT29 and RT112 cell lines are resistant to enfortumab vedotin whereas SUM190PT is sensible. n = 2 biologic replicates were examined in one experiment. C . 4-hour caspase 3/7 cytotoxicity assay indicating a tumor killing of CARTN4 cells against MCF7, HT29, RT112. n=4 healthy donors were examined in three independent experiments. Data in panels B, and C are presented as Mean +/- SEM. P-values were calculated using a 2way ANOVA. Source data are provided in the Source Data file.

    Article Snippet: RT112 cell line was purchased from DSMZ Cell Dive (Braunschweig, Germany).

    Techniques: Expressing, Comparison, Negative Control, Cytotoxicity Assay