one step primescript rt pcr kit (TaKaRa)

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TaKaRa one step primescript rt pcr kit
One Step Primescript Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 2980 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt/One+Step+PrimeScript+RT-PCR+Kit/pmc13635554-313-27-32
Average 97 stars, based on 2980 article reviews

one step primescript rt pcr kit - by Bioz Stars, 2026-10

97/100 stars

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Reverse Transcription:

Article Title: TXNRD1-driven enteric glial remodeling promotes neuronal dysfunction in Hirschsprung disease
Article Snippet: Total RNA was extracted from tissues or cells using TRIzol reagent (Invitrogen, USA). .. Reverse transcription was performed using PrimeScript RT Master Mix (RR036Q, Takara, Japan) to synthesize cDNA. .. Next, the cDNA fragments were amplified and analyzed on an ABI 7500 PCR system (ThermoFisher Scientific, USA).

Article Title: Ac NR1F orchestrates epidermal remodeling to enable host adaptation in Angiostrongylus cantonensis
Article Snippet: Worm morphology was observed using a stereomicroscopy (Leica, Germany), and the size of eggs was calculated using Image J. .. Total RNA was extracted from adult worms using Trizol (Invitrogen, USA) and reverse transcribed into cDNA using the PrimeScript RT Master Mix (TaKaRa, Japan). .. A. cantonensis nr1f gene was amplified using Phanta Max Super-Fidelity DNA Polymerase (Vazyme, China) and cloned into pMD19-T and transformed in Escherichia coli DH5α.

Article Title: Hepatic Lipid Stress Drives Metabolic Dysfunction‐Associated Steatohepatitis Progression through FTO O ‐GlcNAcylation‐Induced m6A Demethylation of HRAS
Article Snippet: Total RNA was isolated from liver tissues or cells using TRIzol reagent (15596018, Invitrogen) according to the manufacturer's instructions. .. cDNA was reverse‐transcribed from RNA using the PrimeScript RT Reagent Kit (RR047A, TaKaRa, Japan). .. Quantitative real‐time PCR was performed using a CFX Connect Real‐Time PCR Detection System (Bio‐Rad) with specific primers (Table ).

Article Title: Ac NR1F orchestrates epidermal remodeling to enable host adaptation in Angiostrongylus cantonensis
Article Snippet: .. Total RNA of A. cantonensis was extracted using Trizol (Invitrogen, USA) and reverse transcribed to cDNA using PrimeScript RT reagent Kit (Takara, Japan) after removing contaminating genomic DNA with DNase I (Sigma, USA). .. qPCR was performed using SYBR Green Real-Time PCR Master Mix (Toyobo, Japan) on a Light Cycler 480 Instrument II (Roche, Switzerland).

Amplification:

Article Title: OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer classification
Article Snippet: .. To identify a reaction chemistry compatible with short-RNA amplification, four commercially available one-step RT-PCR kits—originally designed for long RNA or DNA templates—were evaluated following manufacturer protocols: (i) One Step PrimeScript RT-PCR Kit (TaKaRa, Japan, Cat. no. RC801S), (ii) one-step RT-PCR Kit (Novoprotein, China, Cat. no. E295), (iii) HiScript II U+ One Step qRT-PCR Probe Kit (Vazyme, China, Cat. no. P612-01), (iv) HotStart HiTag & Super M-MuLV one-step qRT-PCR Mix (Fapon, China, Cat. no. MD027). ..

One Step RT-PCR:

Reverse Transcription Polymerase Chain Reaction:

Quantitative RT-PCR:

Synthesized:

Article Title: Engineering Anaerobic Metabolic Branch Points to Control Carbon Allocation Between Wax Ester Synthesis and Succinate Accumulation in Euglena gracilis
Article Snippet: .. Complementary DNA was synthesized using PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio, Japan). .. qRT‐PCR was performed using TB Green Premix Ex Taq II Fast qPCR (Takara Bio, Japan) with a Thermal Cycler Dice Real Time System (Takara Bio, Japan).

Generated:

Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
Article Snippet: Total RNA was purified using TRIzol reagent (Invitrogen, USA) following the manufacturer’s protocol. .. Complementary DNA (cDNA) was generated from equal amounts of RNA using the PrimeScript RT Master Mix kit (Takara, Japan). .. Quantitative RT-PCR was then performed with TB Green Premix Ex Taq II (Takara, Japan) on a LightCycler 480 instrument (Roche, Switzerland).

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