rt pcr (Thermo Fisher)
Name:
SuperScript First Strand Synthesis System for RT PCR
Description:
The SuperScript First Strand Synthesis System for RT PCR is optimized to synthesize first strand cDNA from purified poly A or total RNA The system can be used with as little as 1 ng or as much as 5 µg of total RNA After synthesis the cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations In conjunction with PCR the system can be used to detect the presence of rare messages to quantitate the amount of specific mRNA from small numbers of cells or to clone specific cDNAs without constructing an entire cDNA library The system is flexible allowing the use of any PCR enzyme Combine with AccuPrime Taq DNA Polymerase or Platinum Taq DNA Polymerase for higher specificity PCR or with AccuPrime Pfx DNA Polymerase for high fidelity cloning applications SuperScript II RTThe first strand cDNA synthesis reaction is catalyzed by SuperScript II Reverse Transcriptase RT This enzyme has been engineered to reduce the RNase H activity that degrades mRNA during the first strand reaction resulting in greater full length cDNA synthesis and higher yields of first strand cDNA than obtained with RNase H RTs Because SuperScript II RT is not inhibited significantly by ribosomal and transfer RNA it may be used effectively to synthesize first strand cDNA from a total RNA preparation The enzyme exhibits increased thermal stability and may be used at temperatures up to 50°C Using the SuperScript First Strand Synthesis System for RT PCRThis system has been optimized to synthesize first strand cDNA from varying amounts of starting material The SuperScript II RT concentration has been lowered and RNaseOUT Recombinant RNase Inhibitor has been added to the system as part of this optimization process Additionally reaction conditions have been modified to further increase the sensitivity of the system Using the kit you synthesize first strand cDNA using either total RNA or poly A selected RNA primed with oligo dT random primers or a gene specific primer Then you perform PCR in a separate tube using primers specific for the gene of interest
Catalog Number:
11904018
Price:
None
Applications:
PCR & Real-Time PCR|Reverse Transcription
Category:
Kits and Assays
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Structured Review
The SuperScript First Strand Synthesis System for RT PCR is optimized to synthesize first strand cDNA from purified poly A or total RNA The system can be used with as little as 1 ng or as much as 5 µg of total RNA After synthesis the cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations In conjunction with PCR the system can be used to detect the presence of rare messages to quantitate the amount of specific mRNA from small numbers of cells or to clone specific cDNAs without constructing an entire cDNA library The system is flexible allowing the use of any PCR enzyme Combine with AccuPrime Taq DNA Polymerase or Platinum Taq DNA Polymerase for higher specificity PCR or with AccuPrime Pfx DNA Polymerase for high fidelity cloning applications SuperScript II RTThe first strand cDNA synthesis reaction is catalyzed by SuperScript II Reverse Transcriptase RT This enzyme has been engineered to reduce the RNase H activity that degrades mRNA during the first strand reaction resulting in greater full length cDNA synthesis and higher yields of first strand cDNA than obtained with RNase H RTs Because SuperScript II RT is not inhibited significantly by ribosomal and transfer RNA it may be used effectively to synthesize first strand cDNA from a total RNA preparation The enzyme exhibits increased thermal stability and may be used at temperatures up to 50°C Using the SuperScript First Strand Synthesis System for RT PCRThis system has been optimized to synthesize first strand cDNA from varying amounts of starting material The SuperScript II RT concentration has been lowered and RNaseOUT Recombinant RNase Inhibitor has been added to the system as part of this optimization process Additionally reaction conditions have been modified to further increase the sensitivity of the system Using the kit you synthesize first strand cDNA using either total RNA or poly A selected RNA primed with oligo dT random primers or a gene specific primer Then you perform PCR in a separate tube using primers specific for the gene of interest
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Reverse Transcription Polymerase Chain Reaction:Article Title: Upregulation of SQSTM1/p62 contributes to nickel-induced malignant transformation of human bronchial epithelial cells Article Snippet: .. RT-PCR Cells were exposed to nickel for the indicated time points, and then 5.0 μg total RNA was used for Article Title: M-CSF Induces Vascular Endothelial Growth Factor Production and Angiogenic Activity From Human Monocytes Article Snippet: .. RNA integrity was verified on a 1.5% agarose gel to detect 18S and 28S ribosomal RNA bands at 0.7 and 1.5 kDa, respectively. cDNA was synthesized from monocyte total cellular RNA using the SuperScript First-Strand Synthesis System for Article Title: Tropomyosin 3.1 Association With Actin Stress Fibers is Required for Lens Epithelial to Mesenchymal Transition Article Snippet: .. Reverse transcription was performed on equal amounts of total RNA for each sample using Superscript III First-Strand Synthesis System for Article Title: Effects of the Sri Lankan medicinal plant, Salacia reticulata, in rheumatoid arthritis Article Snippet: .. The Single-strand cDNA was prepared from 1 μg of total RNA using the Article Title: Tropomyosin 3.5 protects the F-actin networks required for tissue biomechanical properties Article Snippet: .. Reverse transcription was performed using the SuperscriptTM III First-Strand Synthesis System for Real-time Polymerase Chain Reaction:Article Title: Bisphosphonates and statins inhibit expression and secretion of MIP-1α via suppression of Ras/MEK/ERK/AML-1A and Ras/PI3K/Akt/AML-1A pathways Article Snippet: .. One microgram of purified total RNA was used for the Synthesized:Article Title: M-CSF Induces Vascular Endothelial Growth Factor Production and Angiogenic Activity From Human Monocytes Article Snippet: .. RNA integrity was verified on a 1.5% agarose gel to detect 18S and 28S ribosomal RNA bands at 0.7 and 1.5 kDa, respectively. cDNA was synthesized from monocyte total cellular RNA using the SuperScript First-Strand Synthesis System for Agarose Gel Electrophoresis:Article Title: M-CSF Induces Vascular Endothelial Growth Factor Production and Angiogenic Activity From Human Monocytes Article Snippet: .. RNA integrity was verified on a 1.5% agarose gel to detect 18S and 28S ribosomal RNA bands at 0.7 and 1.5 kDa, respectively. cDNA was synthesized from monocyte total cellular RNA using the SuperScript First-Strand Synthesis System for Purification:Article Title: Bisphosphonates and statins inhibit expression and secretion of MIP-1α via suppression of Ras/MEK/ERK/AML-1A and Ras/PI3K/Akt/AML-1A pathways Article Snippet: .. One microgram of purified total RNA was used for the |