rt pcr (Thermo Fisher)

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SuperScript First Strand Synthesis System for RT PCR

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The SuperScript First Strand Synthesis System for RT PCR is optimized to synthesize first strand cDNA from purified poly A or total RNA The system can be used with as little as 1 ng or as much as 5 µg of total RNA After synthesis the cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations In conjunction with PCR the system can be used to detect the presence of rare messages to quantitate the amount of specific mRNA from small numbers of cells or to clone specific cDNAs without constructing an entire cDNA library The system is flexible allowing the use of any PCR enzyme Combine with AccuPrime Taq DNA Polymerase or Platinum Taq DNA Polymerase for higher specificity PCR or with AccuPrime Pfx DNA Polymerase for high fidelity cloning applications SuperScript II RTThe first strand cDNA synthesis reaction is catalyzed by SuperScript II Reverse Transcriptase RT This enzyme has been engineered to reduce the RNase H activity that degrades mRNA during the first strand reaction resulting in greater full length cDNA synthesis and higher yields of first strand cDNA than obtained with RNase H RTs Because SuperScript II RT is not inhibited significantly by ribosomal and transfer RNA it may be used effectively to synthesize first strand cDNA from a total RNA preparation The enzyme exhibits increased thermal stability and may be used at temperatures up to 50°C Using the SuperScript First Strand Synthesis System for RT PCRThis system has been optimized to synthesize first strand cDNA from varying amounts of starting material The SuperScript II RT concentration has been lowered and RNaseOUT Recombinant RNase Inhibitor has been added to the system as part of this optimization process Additionally reaction conditions have been modified to further increase the sensitivity of the system Using the kit you synthesize first strand cDNA using either total RNA or poly A selected RNA primed with oligo dT random primers or a gene specific primer Then you perform PCR in a separate tube using primers specific for the gene of interest

Catalog Number:

11904018

Price:

None

Applications:

PCR & Real-Time PCR|Reverse Transcription

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Structured Review

Thermo Fisher rt pcr
The SuperScript First Strand Synthesis System for RT PCR is optimized to synthesize first strand cDNA from purified poly A or total RNA The system can be used with as little as 1 ng or as much as 5 µg of total RNA After synthesis the cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations In conjunction with PCR the system can be used to detect the presence of rare messages to quantitate the amount of specific mRNA from small numbers of cells or to clone specific cDNAs without constructing an entire cDNA library The system is flexible allowing the use of any PCR enzyme Combine with AccuPrime Taq DNA Polymerase or Platinum Taq DNA Polymerase for higher specificity PCR or with AccuPrime Pfx DNA Polymerase for high fidelity cloning applications SuperScript II RTThe first strand cDNA synthesis reaction is catalyzed by SuperScript II Reverse Transcriptase RT This enzyme has been engineered to reduce the RNase H activity that degrades mRNA during the first strand reaction resulting in greater full length cDNA synthesis and higher yields of first strand cDNA than obtained with RNase H RTs Because SuperScript II RT is not inhibited significantly by ribosomal and transfer RNA it may be used effectively to synthesize first strand cDNA from a total RNA preparation The enzyme exhibits increased thermal stability and may be used at temperatures up to 50°C Using the SuperScript First Strand Synthesis System for RT PCRThis system has been optimized to synthesize first strand cDNA from varying amounts of starting material The SuperScript II RT concentration has been lowered and RNaseOUT Recombinant RNase Inhibitor has been added to the system as part of this optimization process Additionally reaction conditions have been modified to further increase the sensitivity of the system Using the kit you synthesize first strand cDNA using either total RNA or poly A selected RNA primed with oligo dT random primers or a gene specific primer Then you perform PCR in a separate tube using primers specific for the gene of interest
https://www.bioz.com/result/rt pcr/product/Thermo Fisher
Average 97 stars, based on 17152 article reviews
Price from $9.99 to $1999.99

rt pcr - by Bioz Stars, 2021-02

97/100 stars

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Reverse Transcription Polymerase Chain Reaction:

Article Title: Upregulation of SQSTM1/p62 contributes to nickel-induced malignant transformation of human bronchial epithelial cells
Article Snippet: .. RT-PCR Cells were exposed to nickel for the indicated time points, and then 5.0 μg total RNA was used for first-strand cDNA synthesis with oligodT (20) primer by SuperScriptTM First-Strand Synthesis system (Invitrogen, 11904018). .. The SQSTM1 and TNF were monitored by PCR.

Article Title: M-CSF Induces Vascular Endothelial Growth Factor Production and Angiogenic Activity From Human Monocytes
Article Snippet: .. RNA integrity was verified on a 1.5% agarose gel to detect 18S and 28S ribosomal RNA bands at 0.7 and 1.5 kDa, respectively. cDNA was synthesized from monocyte total cellular RNA using the SuperScript First-Strand Synthesis System for RT-PCR Kit (Life Technologies). .. The VEGF forward primer, VEGF reverse primer, and the VEGF probe with TAMRA quencher (PE Applied Biosystems) for VEGF mRNA were designed using Primer Express version 1.0 software (ABI PRISM, PerkinElmer, Branchburg, NJ) based on the human VEGF sequence obtained from PubMed (accession no. NM 003376).

Article Title: Tropomyosin 3.1 Association With Actin Stress Fibers is Required for Lens Epithelial to Mesenchymal Transition
Article Snippet: .. Reverse transcription was performed on equal amounts of total RNA for each sample using Superscript III First-Strand Synthesis System for RT-PCR Kit (Thermo Fisher Scientific). .. Semi-quantitative RT-PCR was performed using Hot-Start Taq Blue Mastermix (Thomas Scientific, Swedesboro, NJ, USA) on equivalent amounts of cDNA in a 20 µL reaction and using previously validated mouse Tpm primers.

Article Title: Effects of the Sri Lankan medicinal plant, Salacia reticulata, in rheumatoid arthritis
Article Snippet: .. The Single-strand cDNA was prepared from 1 μg of total RNA using the SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen, Tokyo, Japan). .. Amplification was performed in 10 μl of reaction mixture using EX taq (TAKARA BIO INC, Shiga, Japan).

Article Title: Tropomyosin 3.5 protects the F-actin networks required for tissue biomechanical properties
Article Snippet: .. Reverse transcription was performed using the SuperscriptTM III First-Strand Synthesis System for RT-PCR kit (Thermo Fisher Scientific) from an equal amount of total RNA for each sample. .. PCR was performed with the same amount of cDNA from each sample in a 20 µl volume reaction.

Real-time Polymerase Chain Reaction:

Article Title: Bisphosphonates and statins inhibit expression and secretion of MIP-1α via suppression of Ras/MEK/ERK/AML-1A and Ras/PI3K/Akt/AML-1A pathways
Article Snippet: .. One microgram of purified total RNA was used for the real-time PCR analysis with the SuperScript First-Strand Synthesis System (Invitrogen). cDNA was subjected to quantitative real-time PCR by using SYBR Premix Ex Taq (Takara Biomedical; Siga, Japan) and the Thermal Cycler Dice Real Time system (Takara Biomedical) in a 96-well plate according to the manufacturer’s instructions. .. The PCR conditions for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), MIP-1α, AML-1A, CREB, NF-AT, and C/ERBβ were 94°C for 2 min; followed by 40 cycles of 94°C for 0.5 min, 50°C for 0.5 min, and 72°C for 0.5 min.