human bladder cancer cell lines  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human bladder cancer cell lines
    Human Bladder Cancer Cell Lines, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rpmi high glucose rt 4 and rt 112 were purchased from the cell lines service  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH rpmi high glucose rt 4 and rt 112 were purchased from the cell lines service
    Rpmi High Glucose Rt 4 And Rt 112 Were Purchased From The Cell Lines Service, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human bladder cancer cell lines  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human bladder cancer cell lines
    Human Bladder Cancer Cell Lines, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rt 112  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH rt 112
    Rt 112, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rt 112  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH rt 112
    ROS/RNS profile of kINPen plasma and cellular oxidation. ( a ) Schematic overview of ROS/RNS characterization in plasma gas and liquid phase. ( b ) Optical emission spectroscopy (OES) profile of the kINPen plasma jet indicating the presence of hydroxyl radicals ( . OH), molecular nitrogen (N 2 ) as part of the second positive system of nitrogen, and excited argon (Ar) peaks (n = 10). ( c ) pH in gas plasma-oxidized NaCl (n = 3). ( d-f ) Quantification of hydrogen peroxide (H 2 O 2 ; d ), nitrite (NO 2 – ; e ), and nitrate (NO 3 – ; f ) in gas plasma-oxidized liquids immediately (bars) and 1 h after gas plasma treatment in the presence (circle) or absence (triangle) of cancer cells (n = 3); bar graphs show mean ± standard error of the mean (SEM). ( g ) Representative images of intracellular ROS evaluated via DCF fluorescence. ( h ) Quantitative image analysis of DCF fluorescence intensities immediately, 6 h, and 12 h after gas plasma treatment in malignant ( I ) <t>RT-112,</t> ( II ) SCaBER, and ( III ) T24 cells, and ( IV ) CLS cells normalized to control (n = 3); graphs show mean and biological replicates; statistical analysis was performed using one-way analysis of variance (ANOVA) with Dunnett post-hoc testing (***p < 0.001). Scale bar = 100 µm.
    Rt 112, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Medical gas plasma augments bladder cancer cell toxicity in preclinical models and patient-derived tumor tissues"

    Article Title: Medical gas plasma augments bladder cancer cell toxicity in preclinical models and patient-derived tumor tissues

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2022.07.012

    ROS/RNS profile of kINPen plasma and cellular oxidation. ( a ) Schematic overview of ROS/RNS characterization in plasma gas and liquid phase. ( b ) Optical emission spectroscopy (OES) profile of the kINPen plasma jet indicating the presence of hydroxyl radicals ( . OH), molecular nitrogen (N 2 ) as part of the second positive system of nitrogen, and excited argon (Ar) peaks (n = 10). ( c ) pH in gas plasma-oxidized NaCl (n = 3). ( d-f ) Quantification of hydrogen peroxide (H 2 O 2 ; d ), nitrite (NO 2 – ; e ), and nitrate (NO 3 – ; f ) in gas plasma-oxidized liquids immediately (bars) and 1 h after gas plasma treatment in the presence (circle) or absence (triangle) of cancer cells (n = 3); bar graphs show mean ± standard error of the mean (SEM). ( g ) Representative images of intracellular ROS evaluated via DCF fluorescence. ( h ) Quantitative image analysis of DCF fluorescence intensities immediately, 6 h, and 12 h after gas plasma treatment in malignant ( I ) RT-112, ( II ) SCaBER, and ( III ) T24 cells, and ( IV ) CLS cells normalized to control (n = 3); graphs show mean and biological replicates; statistical analysis was performed using one-way analysis of variance (ANOVA) with Dunnett post-hoc testing (***p < 0.001). Scale bar = 100 µm.
    Figure Legend Snippet: ROS/RNS profile of kINPen plasma and cellular oxidation. ( a ) Schematic overview of ROS/RNS characterization in plasma gas and liquid phase. ( b ) Optical emission spectroscopy (OES) profile of the kINPen plasma jet indicating the presence of hydroxyl radicals ( . OH), molecular nitrogen (N 2 ) as part of the second positive system of nitrogen, and excited argon (Ar) peaks (n = 10). ( c ) pH in gas plasma-oxidized NaCl (n = 3). ( d-f ) Quantification of hydrogen peroxide (H 2 O 2 ; d ), nitrite (NO 2 – ; e ), and nitrate (NO 3 – ; f ) in gas plasma-oxidized liquids immediately (bars) and 1 h after gas plasma treatment in the presence (circle) or absence (triangle) of cancer cells (n = 3); bar graphs show mean ± standard error of the mean (SEM). ( g ) Representative images of intracellular ROS evaluated via DCF fluorescence. ( h ) Quantitative image analysis of DCF fluorescence intensities immediately, 6 h, and 12 h after gas plasma treatment in malignant ( I ) RT-112, ( II ) SCaBER, and ( III ) T24 cells, and ( IV ) CLS cells normalized to control (n = 3); graphs show mean and biological replicates; statistical analysis was performed using one-way analysis of variance (ANOVA) with Dunnett post-hoc testing (***p < 0.001). Scale bar = 100 µm.

    Techniques Used: Spectroscopy, Fluorescence

    Gas plasma treatment reduced metabolic activity, motility, and viability in urothelial cancer cell lines. ( a ) Schematic overview of gas plasma treatment performed in 2D in vitro experiments. ( b ) representative images of resazurin metabolization. ( c ) Metabolic activity quantification normalized to respective controls indicating IC 50 values (box) calculated from non-linear regression of log-transformed x-values (n = 8); graph shows mean. ( d ) Representative flow cytometry intensity histogram of caspase 3/7 activation. ( e ) Apoptosis quantification normalized to respective controls (n = 5); heat map shows mean; statistical analysis was performed using one-way analysis of variance (ANOVA) with Dunnett post-hoc testing (*p < 0.05, **p < 0.01; ***p < 0.001). ( f ) Representative images of DAPI and Mitotracker Red staining. ( g-h ) Quantification of ( g ) percent DAPI + cells and ( h ) mitochondrial membrane potential intensity evaluated until 24 h after gas plasma treatment normalized against the 0 h control (n = 3); graphs show mean. ( i ) 10–90 % percentile box plots (mean indicated by + ) showing quantification of cellular motility for ( I ) RT-112, ( II ) SCaBER, and ( III ) T24 cells (n = 3 with 5 technical replicates each); statistical analysis was performed using unpaired t -test (***p < 0.001). ns = non-significant. Scale bar = 100 µm (f).
    Figure Legend Snippet: Gas plasma treatment reduced metabolic activity, motility, and viability in urothelial cancer cell lines. ( a ) Schematic overview of gas plasma treatment performed in 2D in vitro experiments. ( b ) representative images of resazurin metabolization. ( c ) Metabolic activity quantification normalized to respective controls indicating IC 50 values (box) calculated from non-linear regression of log-transformed x-values (n = 8); graph shows mean. ( d ) Representative flow cytometry intensity histogram of caspase 3/7 activation. ( e ) Apoptosis quantification normalized to respective controls (n = 5); heat map shows mean; statistical analysis was performed using one-way analysis of variance (ANOVA) with Dunnett post-hoc testing (*p < 0.05, **p < 0.01; ***p < 0.001). ( f ) Representative images of DAPI and Mitotracker Red staining. ( g-h ) Quantification of ( g ) percent DAPI + cells and ( h ) mitochondrial membrane potential intensity evaluated until 24 h after gas plasma treatment normalized against the 0 h control (n = 3); graphs show mean. ( i ) 10–90 % percentile box plots (mean indicated by + ) showing quantification of cellular motility for ( I ) RT-112, ( II ) SCaBER, and ( III ) T24 cells (n = 3 with 5 technical replicates each); statistical analysis was performed using unpaired t -test (***p < 0.001). ns = non-significant. Scale bar = 100 µm (f).

    Techniques Used: Activity Assay, In Vitro, Transformation Assay, Flow Cytometry, Activation Assay, Staining

    Gas plasma exposure elicits cytotoxicity in 3D urothelial cancer spheroids and vascularized tumor tissues in ovo. ( a ) Schematic overview of the experimental 3D spheroid procedure and representative brightfield images of successful tumor spheroid formation of the tumor cell lines but not CLS cells. ( b ) Representative images of algorithm-based 3D tumor spheroid toxicity image analysis. ( c ) Relative growth change of urothelial cancer spheroids after gas plasma treatment compared to argon gas control (n = 3); bar graph shows mean ± standard error of the mean (SEM). ( d ) Quantification of sytox green-positive spots 24 h and 48 h after gas plasma treatment of ( I ) RT-112, ( II ) SCaBER, and ( III ) T24 spheroids; graphs show mean ± SEM; statistical analysis was performed using one-way analysis of variance (ANOVA) with Dunnett post-hoc testing (*p < 0.05, **p < 0.01; ***p < 0.001). ( e ) Schematic overview of in ovo experimental procedures (left) showing representative images of argon gas and gas plasma treatment performed in ovo (right). ( f–h ) Representative pictures of excised in ovo tumors followed by assessment of tumor weight of RT-112 (n = 11 (control), n = 8 (plasma); f ), SCaBER (n = 9 (control), n = 8 (plasma); g ), and T24 (n = 7 (control), n = 6 (plasma); h ) cells; graphs show mean and biological replicates; statistical analysis was performed using unpaired t -test (**p < 0.01; ***p < 0.001). Scale bar = 100 µm (b).
    Figure Legend Snippet: Gas plasma exposure elicits cytotoxicity in 3D urothelial cancer spheroids and vascularized tumor tissues in ovo. ( a ) Schematic overview of the experimental 3D spheroid procedure and representative brightfield images of successful tumor spheroid formation of the tumor cell lines but not CLS cells. ( b ) Representative images of algorithm-based 3D tumor spheroid toxicity image analysis. ( c ) Relative growth change of urothelial cancer spheroids after gas plasma treatment compared to argon gas control (n = 3); bar graph shows mean ± standard error of the mean (SEM). ( d ) Quantification of sytox green-positive spots 24 h and 48 h after gas plasma treatment of ( I ) RT-112, ( II ) SCaBER, and ( III ) T24 spheroids; graphs show mean ± SEM; statistical analysis was performed using one-way analysis of variance (ANOVA) with Dunnett post-hoc testing (*p < 0.05, **p < 0.01; ***p < 0.001). ( e ) Schematic overview of in ovo experimental procedures (left) showing representative images of argon gas and gas plasma treatment performed in ovo (right). ( f–h ) Representative pictures of excised in ovo tumors followed by assessment of tumor weight of RT-112 (n = 11 (control), n = 8 (plasma); f ), SCaBER (n = 9 (control), n = 8 (plasma); g ), and T24 (n = 7 (control), n = 6 (plasma); h ) cells; graphs show mean and biological replicates; statistical analysis was performed using unpaired t -test (**p < 0.01; ***p < 0.001). Scale bar = 100 µm (b).

    Techniques Used: In Ovo

    rt-112 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH rt-112 cells
    Rt 112 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rt112 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH rt112 cells
    Rt112 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rt 112  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH rt 112
    Rt 112, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    treatments rt 112 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH treatments rt 112 cells
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    treatments rt 112 cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH treatments rt 112 cells
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