rpmi1640 media  (Thermo Fisher)


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  • 99
    Name:
    RPMI 1640 Medium Dutch modification 1X
    Description:
    Roswell Park Memorial Institute RPMI 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa Jurkat MCF 7 PC12 PBMC astrocytes and carcinomas We offer a variety of Gibco RPMI 1640 modifications for a range of cell culture applications Find the right formulation using the media selector tool This RPMI is modified as follows WithWithoutPhenol RedL glutamineHEPESLow sodium bicarbonate The complete formulation is available Gibco RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins RPMI 1640 contains biotin vitamin B12 and PABA which are not found in Eagle s Minimal Essential Medium or Dulbecco s Modified Eagle Medium In addition the vitamins inositol and choline are present in very high concentrations This RPMI formulation has the Dutch modification addition of HEPES with sodium bicarbonate lowered to 1 g L Product Intended UseFor Research Use Only Not for use in diagnostic procedures cGMP Manufacturing and Quality SystemGibco RPMI 1640 is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard RPMI 1640 contains no proteins lipids or growth factors Therefore RPMI 1640 requires supplementation commonly with 10 Fetal Bovine Serum FBS RPMI 1640 uses a sodium bicarbonate buffer system 1 0 g L and therefore requires a 5 7 CO2 environment to maintain physiological pH
    Catalog Number:
    22409031
    Price:
    None
    Applications:
    Cell Culture|Mammalian Cell Culture
    Category:
    Cell Culture Transfection Reagents
    Buy from Supplier


    Structured Review

    Thermo Fisher rpmi1640 media
    The synergistic anticancer effects of vorinostat and EGCG were measured by cytotoxic and growth inhibition responses. A 3 × 10 4 aliquot of cells for the cell cytotoxicity assay and 3 × 10 3 cells for growth inhibition assay were seeded in 96-well plates. <t>RPMI1640</t> media supplemented 10% FBS was used to asses tumor cell growth inhibition, whereas serum-free media were used for cell cytotoxicity assay. Single agent treatment: (a) and (b), EGCG; (c) and (d), vorinostat. Combined vorinostat and EGCG: (e) and (f). Growth inhibition: (a), (c), and (e). Cell cytotoxicity: (b), (d), and (f). * P
    Roswell Park Memorial Institute RPMI 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa Jurkat MCF 7 PC12 PBMC astrocytes and carcinomas We offer a variety of Gibco RPMI 1640 modifications for a range of cell culture applications Find the right formulation using the media selector tool This RPMI is modified as follows WithWithoutPhenol RedL glutamineHEPESLow sodium bicarbonate The complete formulation is available Gibco RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins RPMI 1640 contains biotin vitamin B12 and PABA which are not found in Eagle s Minimal Essential Medium or Dulbecco s Modified Eagle Medium In addition the vitamins inositol and choline are present in very high concentrations This RPMI formulation has the Dutch modification addition of HEPES with sodium bicarbonate lowered to 1 g L Product Intended UseFor Research Use Only Not for use in diagnostic procedures cGMP Manufacturing and Quality SystemGibco RPMI 1640 is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard RPMI 1640 contains no proteins lipids or growth factors Therefore RPMI 1640 requires supplementation commonly with 10 Fetal Bovine Serum FBS RPMI 1640 uses a sodium bicarbonate buffer system 1 0 g L and therefore requires a 5 7 CO2 environment to maintain physiological pH
    https://www.bioz.com/result/rpmi1640 media/product/Thermo Fisher
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 media - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Synergistic Anticancer Effects of Vorinostat and Epigallocatechin-3-Gallate against HuCC-T1 Human Cholangiocarcinoma Cells"

    Article Title: Synergistic Anticancer Effects of Vorinostat and Epigallocatechin-3-Gallate against HuCC-T1 Human Cholangiocarcinoma Cells

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2013/185158

    The synergistic anticancer effects of vorinostat and EGCG were measured by cytotoxic and growth inhibition responses. A 3 × 10 4 aliquot of cells for the cell cytotoxicity assay and 3 × 10 3 cells for growth inhibition assay were seeded in 96-well plates. RPMI1640 media supplemented 10% FBS was used to asses tumor cell growth inhibition, whereas serum-free media were used for cell cytotoxicity assay. Single agent treatment: (a) and (b), EGCG; (c) and (d), vorinostat. Combined vorinostat and EGCG: (e) and (f). Growth inhibition: (a), (c), and (e). Cell cytotoxicity: (b), (d), and (f). * P
    Figure Legend Snippet: The synergistic anticancer effects of vorinostat and EGCG were measured by cytotoxic and growth inhibition responses. A 3 × 10 4 aliquot of cells for the cell cytotoxicity assay and 3 × 10 3 cells for growth inhibition assay were seeded in 96-well plates. RPMI1640 media supplemented 10% FBS was used to asses tumor cell growth inhibition, whereas serum-free media were used for cell cytotoxicity assay. Single agent treatment: (a) and (b), EGCG; (c) and (d), vorinostat. Combined vorinostat and EGCG: (e) and (f). Growth inhibition: (a), (c), and (e). Cell cytotoxicity: (b), (d), and (f). * P

    Techniques Used: Inhibition, Cytotoxicity Assay, Growth Inhibition Assay

    Related Articles

    other:

    Article Title: High Expression of IGFBP7 in Fibroblasts Induced by Colorectal Cancer Cells Is Co-Regulated by TGF-? and Wnt Signaling in a Smad2/3-Dvl2/3-Dependent Manner
    Article Snippet: Preparation of SW620 supernatant CRC cell line SW620 was plated in culture flasks (8×105 cells) in RPMI1640 supplemented with Penicillin-Streptomycin Solution (as above), and 10% heat-inactivated fetal bovine serum (Gibco, USA).

    Article Title: Beyond Warburg effect - dual metabolic nature of cancer cells
    Article Snippet: Cell proliferation, glucose consumption and lactate generation 4T1, Bcap37, A549 and HeLa cells are maintained in RPMI-1640 (Invitrogen) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin/streptomycin and 2 mM L-glutamine (complete RPMI).

    Cell Culture:

    Article Title: High Expression of IGFBP7 in Fibroblasts Induced by Colorectal Cancer Cells Is Co-Regulated by TGF-? and Wnt Signaling in a Smad2/3-Dvl2/3-Dependent Manner
    Article Snippet: .. Cell cultures Fibroblast (HELF) and CRC cell line SW620 were both cultured in RPMI1640 (Gibco, USA) supplemented with Penicillin-Streptomycin Solution (100 U/ml penicillin, 0.1 mg/ml streptomycin, Gibco, USA), 10% heat-inactivated fetal bovine serum, 50% supernatant of CRC cell line SW620. ..

    Article Title: The Use of Porous Scaffold as a Tumor Model
    Article Snippet: .. Standard Monolayer Culture NCI-H460 cells were cultured in RPMI1640 (GIBCO, Life Technologies, Victoria, Australia) supplemented with 2 mM glutamine and 5% Fetal Calf Serum (FCS, Interpath Services, Melbourne, Australia) at 37°C in 5% CO2 /humidified air. ..

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    Thermo Fisher cardiomyocyte differentiation media
    iPSC-derived cardiomyocytes elicit a cellular response to hypoxia. (A) Experimental design of the study. Cardiomyocytes differentiated from iPSCs (iPSC-CMs) from 15 Yoruba individuals were cultured in normoxic conditions (10% oxygen - condition A) and subjected to 6 hours of hypoxia (1% oxygen - condition B) followed by 6 and 24 hours of re-oxygenation (10% oxygen - conditions C and D). Immunocytochemistry of a representative <t>cardiomyocyte</t> culture where green: TNNT2; blue: nuclei. (B) Peri-cellular oxygen levels of each condition. Each point represents one individual undergoing the oxygen stress experiment. (C) Relative levels of BNP, a marker for cardiac stress, released into cell culture media. Asterisk denotes a statistically significant difference in BNP release (* p
    Cardiomyocyte Differentiation Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cardiomyocyte differentiation media/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cardiomyocyte differentiation media - by Bioz Stars, 2020-09
    88/100 stars
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    99
    Thermo Fisher rpmi1640 media
    iPSC-derived cardiomyocytes elicit a cellular response to hypoxia. (A) Experimental design of the study. Cardiomyocytes differentiated from iPSCs (iPSC-CMs) from 15 Yoruba individuals were cultured in normoxic conditions (10% oxygen - condition A) and subjected to 6 hours of hypoxia (1% oxygen - condition B) followed by 6 and 24 hours of re-oxygenation (10% oxygen - conditions C and D). Immunocytochemistry of a representative <t>cardiomyocyte</t> culture where green: TNNT2; blue: nuclei. (B) Peri-cellular oxygen levels of each condition. Each point represents one individual undergoing the oxygen stress experiment. (C) Relative levels of BNP, a marker for cardiac stress, released into cell culture media. Asterisk denotes a statistically significant difference in BNP release (* p
    Rpmi1640 Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1481 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi1640 media/product/Thermo Fisher
    Average 99 stars, based on 1481 article reviews
    Price from $9.99 to $1999.99
    rpmi1640 media - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    iPSC-derived cardiomyocytes elicit a cellular response to hypoxia. (A) Experimental design of the study. Cardiomyocytes differentiated from iPSCs (iPSC-CMs) from 15 Yoruba individuals were cultured in normoxic conditions (10% oxygen - condition A) and subjected to 6 hours of hypoxia (1% oxygen - condition B) followed by 6 and 24 hours of re-oxygenation (10% oxygen - conditions C and D). Immunocytochemistry of a representative cardiomyocyte culture where green: TNNT2; blue: nuclei. (B) Peri-cellular oxygen levels of each condition. Each point represents one individual undergoing the oxygen stress experiment. (C) Relative levels of BNP, a marker for cardiac stress, released into cell culture media. Asterisk denotes a statistically significant difference in BNP release (* p

    Journal: bioRxiv

    Article Title: Dynamic effects of genetic variation on gene expression revealed following hypoxic stress in cardiomyocytes

    doi: 10.1101/2020.03.28.012823

    Figure Lengend Snippet: iPSC-derived cardiomyocytes elicit a cellular response to hypoxia. (A) Experimental design of the study. Cardiomyocytes differentiated from iPSCs (iPSC-CMs) from 15 Yoruba individuals were cultured in normoxic conditions (10% oxygen - condition A) and subjected to 6 hours of hypoxia (1% oxygen - condition B) followed by 6 and 24 hours of re-oxygenation (10% oxygen - conditions C and D). Immunocytochemistry of a representative cardiomyocyte culture where green: TNNT2; blue: nuclei. (B) Peri-cellular oxygen levels of each condition. Each point represents one individual undergoing the oxygen stress experiment. (C) Relative levels of BNP, a marker for cardiac stress, released into cell culture media. Asterisk denotes a statistically significant difference in BNP release (* p

    Article Snippet: Briefly, on Day 0, iPSC lines at 70-100% confluence in 100 mm plates were treated with 12 μM GSK3 inhibitor CHIR99021 trihydrochloride (4953, Tocris Bioscience, Bristol, UK) in 12 ml Cardiomyocyte Differentiation Media [500 mL RPMI1640 (15-040-CM ThermoFisher Scientific), 10 mL B-27 Minus Insulin (A1895601, ThermoFisher Scientific), 5 mL Glutamax (35050-061, ThermoFisher Scientific), and 5 mL Penicillin/Streptomycin)], and a 1:100 dilution of Matrigel.

    Techniques: Derivative Assay, Cell Culture, Immunocytochemistry, Marker