rpmi 1640  (GE Healthcare)

 
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    Name:
    RPMI 1640 with L glutamine
    Description:

    Catalog Number:
    sh30011.02
    Price:
    46.16 USD
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    Structured Review

    GE Healthcare rpmi 1640
    (A) Cross-reactivity of the anti-FIV factor(s). PBMC infected in vitro with FIV-Pet for 10 days were cultured for 11 days with supernatant collected from FIV-PPR-infected cat 306 PBMC (306 Sup) which had been stimulated with autologous APC for 2 weeks. Control cells were cultured with complete RPMI 1640. Supernatant and medium were added to the infected cells every 3 days. FIV replication determined by an FIV p24 ELISA for cells cultured with supernatant was compared with that for control cells cultured without supernatant. The data are representative of three different experiments. (B) Cell specificity of the anti-FIV factor(s). Chronically FIV-Pet-infected CrFK cells (10 5 /ml) were cultured for 4 days with supernatant collected from FIV-PPR-infected cat 306 PBMC (306 Sup) which had been stimulated with autologous APC for 2 weeks. Control cells were cultured with complete DMEM. Supernatant and medium were added to cells every 2 days. FIV replication determined by an FIV capsid antigen ELISA for cells cultured with supernatant was compared with that for control cells cultured without supernatant. The data are representative of three separate experiments. Error bars in both panels indicate standard deviations.

    https://www.bioz.com/result/rpmi 1640/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rpmi 1640 - by Bioz Stars, 2021-03
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    Images

    1) Product Images from "Anti-Feline Immunodeficiency Virus (FIV) Soluble Factor(s) Produced from Antigen-Stimulated Feline CD8+ T Lymphocytes Suppresses FIV Replication"

    Article Title: Anti-Feline Immunodeficiency Virus (FIV) Soluble Factor(s) Produced from Antigen-Stimulated Feline CD8+ T Lymphocytes Suppresses FIV Replication

    Journal: Journal of Virology

    doi:

    (A) Cross-reactivity of the anti-FIV factor(s). PBMC infected in vitro with FIV-Pet for 10 days were cultured for 11 days with supernatant collected from FIV-PPR-infected cat 306 PBMC (306 Sup) which had been stimulated with autologous APC for 2 weeks. Control cells were cultured with complete RPMI 1640. Supernatant and medium were added to the infected cells every 3 days. FIV replication determined by an FIV p24 ELISA for cells cultured with supernatant was compared with that for control cells cultured without supernatant. The data are representative of three different experiments. (B) Cell specificity of the anti-FIV factor(s). Chronically FIV-Pet-infected CrFK cells (10 5 /ml) were cultured for 4 days with supernatant collected from FIV-PPR-infected cat 306 PBMC (306 Sup) which had been stimulated with autologous APC for 2 weeks. Control cells were cultured with complete DMEM. Supernatant and medium were added to cells every 2 days. FIV replication determined by an FIV capsid antigen ELISA for cells cultured with supernatant was compared with that for control cells cultured without supernatant. The data are representative of three separate experiments. Error bars in both panels indicate standard deviations.
    Figure Legend Snippet: (A) Cross-reactivity of the anti-FIV factor(s). PBMC infected in vitro with FIV-Pet for 10 days were cultured for 11 days with supernatant collected from FIV-PPR-infected cat 306 PBMC (306 Sup) which had been stimulated with autologous APC for 2 weeks. Control cells were cultured with complete RPMI 1640. Supernatant and medium were added to the infected cells every 3 days. FIV replication determined by an FIV p24 ELISA for cells cultured with supernatant was compared with that for control cells cultured without supernatant. The data are representative of three different experiments. (B) Cell specificity of the anti-FIV factor(s). Chronically FIV-Pet-infected CrFK cells (10 5 /ml) were cultured for 4 days with supernatant collected from FIV-PPR-infected cat 306 PBMC (306 Sup) which had been stimulated with autologous APC for 2 weeks. Control cells were cultured with complete DMEM. Supernatant and medium were added to cells every 2 days. FIV replication determined by an FIV capsid antigen ELISA for cells cultured with supernatant was compared with that for control cells cultured without supernatant. The data are representative of three separate experiments. Error bars in both panels indicate standard deviations.

    Techniques Used: Infection, In Vitro, Positron Emission Tomography, Cell Culture, Enzyme-linked Immunosorbent Assay

    Kinetics of FIV replication in PBMC of infected cats. PBMC of uninfected and FIV-infected cats were cultured at a concentration of 5 × 10 5 /ml in RPMI-1640 supplemented with 100 U of hr IL-2 per ml after ConA stimulation for the first 3 days. The culture supernatants were harvested on the indicated days of culturing. FIV replication was measured by use of an FIV capsid antigen (p24) ELISA. Data are for uninfected cat AUS3 (diamonds) and FIV-infected cats 306 (circles) and 308 (squares).
    Figure Legend Snippet: Kinetics of FIV replication in PBMC of infected cats. PBMC of uninfected and FIV-infected cats were cultured at a concentration of 5 × 10 5 /ml in RPMI-1640 supplemented with 100 U of hr IL-2 per ml after ConA stimulation for the first 3 days. The culture supernatants were harvested on the indicated days of culturing. FIV replication was measured by use of an FIV capsid antigen (p24) ELISA. Data are for uninfected cat AUS3 (diamonds) and FIV-infected cats 306 (circles) and 308 (squares).

    Techniques Used: Infection, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Kinetics of FIV replication in CD4 + and CD8 + T lymphocytes of infected cats. CD4 + and CD8 + T cells were prepared by the panning method and stimulated with ConA for the first 3 days. Cells were cultured in complete RPMI-1640 supplemented with 100 U of hr IL-2 per ml at a concentration of 5 × 10 5 /ml. FIV replication was measured in the culture supernatants by an ELISA. Data are for FIV-infected cat 306 CD4 + (closed circles) and CD8 + (open circles) T cells and FIV-infected cat 308 CD4 + (closed squares) and CD8 + (open squares) T cells.
    Figure Legend Snippet: Kinetics of FIV replication in CD4 + and CD8 + T lymphocytes of infected cats. CD4 + and CD8 + T cells were prepared by the panning method and stimulated with ConA for the first 3 days. Cells were cultured in complete RPMI-1640 supplemented with 100 U of hr IL-2 per ml at a concentration of 5 × 10 5 /ml. FIV replication was measured in the culture supernatants by an ELISA. Data are for FIV-infected cat 306 CD4 + (closed circles) and CD8 + (open circles) T cells and FIV-infected cat 308 CD4 + (closed squares) and CD8 + (open squares) T cells.

    Techniques Used: Infection, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay

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    Centrifugation:

    Article Title: Analysis of the Interaction between Globular Head Modules of Human C1q and Its Candidate Receptor gC1qR
    Article Snippet: .. To isolate monocytes, blood in RPMI 1640 was separated on a Ficol column (Ficol-Plaque Plus, GE healthcare) by centrifugation at 2000 rpm for 16 min at room temperature. ..

    Cell Culture:

    Article Title: Combining angiotensin II blockade and renin receptor inhibition results in enhanced antifibrotic effect in experimental nephritis
    Article Snippet: Glomeruli from individual rats were isolated by graded sieving with 150-, 125-, and 75-μm mesh metal sieves as described previously ( , ). .. Five thousand glomeruli/well were resuspended and cultured in a six-well-plate with 2 ml of RPMI 1640 supplemented with 2.5% FBS (Hyclone Laboratory, Logan, UT), 100 U/ml penicillin, 100 μg/ml streptomycin, 0.1 U/ml insulin, and 25 mmol/l HEPES buffer. ..

    Article Title: The Ability of Different Ketohexoses to Alter Apo-A-I Structure and Function In Vitro and to Induce Hepatosteatosis, Oxidative Stress, and Impaired Plasma Lipid Profile in Hyperlipidemic Zebrafish
    Article Snippet: Subsequently, it was filtered (0.2 μ m) and investigated by using a thiobarbituric acid reacting substances (TBARS) assay to establish the degree of oxidation [ ]. .. Cell Culture The human monocyte cell line, THP-1, was acquired from the (ATCC, #TIB-202™; Manassas, VA, USA) and sustained in RPMI1640 (Hyclone, Logan, Utah) with 10% fetal bovine serum (FBS) supplemented. .. The cell line below 20 passages were used and incubated in phorbol 12-myristate 13-acetate (PMA; final 150 nM) supplemented medium in 24-well plates for 48 hours at 37°C in a humidified incubator (5% CO2 ) to induce macrophage differentiation.

    Concentration Assay:

    Article Title: Paeoniflorin suppresses TGF-β mediated epithelial-mesenchymal transition in pulmonary fibrosis through a Smad-dependent pathway
    Article Snippet: To identify the mechanism by which paeoniflorin suppresses the synthesis of type I collagen in PF, the present study was aimed at investigating the effect of paeoniflorin on TGF-β mediated pulmonary EMT using in vivo and in vitro assays. .. Paeoniflorin (purity > 95%, MW : 480.45, dissolved in DMSO to a final concentration lower than 0.1%) was purchased from Nanjing Zelang Medical Technology Co, Ltd (Nanjing, China); prednisone acetate was purchased from Zhejiang Xianju Pharmaceutical Co, Ltd (Taizhou, China); bleomycin hydrochloride (BLM) was purchased from Nippon Kayaku (Tokyo, Japan); RPMI-1640 and fetal bovine serum (FBS) were purchased from HyClone (Logan, USA); recombinant human TGF-β1 was purchased from R & D Systems (Minneapolis, USA); E-cadherin, Smad2/3, p-Smad2 and p-Smad3 antibodies were purchased from Cell Signaling Technology (Boston, MA, USA); α-SMA antibodies were purchased from Epitomics (Burlingame, CA, USA); FSP-1, Smad7, ALK5 and vimentin antibodies were purchased from Bioworld Technology, Inc (Minneapolis, USA); Akt, p-Akt; JNK, p-JNK, ERK, p-ERK, p38, p-p38 and GAPDH antibodies were purchased from Kangchen Biotech (Shanghai, China); type I collagen ELISA kits were purchased from Abcam (Cambridge, UK); iScript cDNA synthesis kits and SsoFast EvaGreen Supermix were purchased from Bio-Rad (Hercules, USA); and TRIzol reagent was purchased from TransGen Biotech (Beijing, China). .. Male ICR mice weighing 22±2 g were purchased from the Comparative Medicine Centre of Yangzhou University (Yangzhou, China).

    Recombinant:

    Article Title: Paeoniflorin suppresses TGF-β mediated epithelial-mesenchymal transition in pulmonary fibrosis through a Smad-dependent pathway
    Article Snippet: To identify the mechanism by which paeoniflorin suppresses the synthesis of type I collagen in PF, the present study was aimed at investigating the effect of paeoniflorin on TGF-β mediated pulmonary EMT using in vivo and in vitro assays. .. Paeoniflorin (purity > 95%, MW : 480.45, dissolved in DMSO to a final concentration lower than 0.1%) was purchased from Nanjing Zelang Medical Technology Co, Ltd (Nanjing, China); prednisone acetate was purchased from Zhejiang Xianju Pharmaceutical Co, Ltd (Taizhou, China); bleomycin hydrochloride (BLM) was purchased from Nippon Kayaku (Tokyo, Japan); RPMI-1640 and fetal bovine serum (FBS) were purchased from HyClone (Logan, USA); recombinant human TGF-β1 was purchased from R & D Systems (Minneapolis, USA); E-cadherin, Smad2/3, p-Smad2 and p-Smad3 antibodies were purchased from Cell Signaling Technology (Boston, MA, USA); α-SMA antibodies were purchased from Epitomics (Burlingame, CA, USA); FSP-1, Smad7, ALK5 and vimentin antibodies were purchased from Bioworld Technology, Inc (Minneapolis, USA); Akt, p-Akt; JNK, p-JNK, ERK, p-ERK, p38, p-p38 and GAPDH antibodies were purchased from Kangchen Biotech (Shanghai, China); type I collagen ELISA kits were purchased from Abcam (Cambridge, UK); iScript cDNA synthesis kits and SsoFast EvaGreen Supermix were purchased from Bio-Rad (Hercules, USA); and TRIzol reagent was purchased from TransGen Biotech (Beijing, China). .. Male ICR mice weighing 22±2 g were purchased from the Comparative Medicine Centre of Yangzhou University (Yangzhou, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Paeoniflorin suppresses TGF-β mediated epithelial-mesenchymal transition in pulmonary fibrosis through a Smad-dependent pathway
    Article Snippet: To identify the mechanism by which paeoniflorin suppresses the synthesis of type I collagen in PF, the present study was aimed at investigating the effect of paeoniflorin on TGF-β mediated pulmonary EMT using in vivo and in vitro assays. .. Paeoniflorin (purity > 95%, MW : 480.45, dissolved in DMSO to a final concentration lower than 0.1%) was purchased from Nanjing Zelang Medical Technology Co, Ltd (Nanjing, China); prednisone acetate was purchased from Zhejiang Xianju Pharmaceutical Co, Ltd (Taizhou, China); bleomycin hydrochloride (BLM) was purchased from Nippon Kayaku (Tokyo, Japan); RPMI-1640 and fetal bovine serum (FBS) were purchased from HyClone (Logan, USA); recombinant human TGF-β1 was purchased from R & D Systems (Minneapolis, USA); E-cadherin, Smad2/3, p-Smad2 and p-Smad3 antibodies were purchased from Cell Signaling Technology (Boston, MA, USA); α-SMA antibodies were purchased from Epitomics (Burlingame, CA, USA); FSP-1, Smad7, ALK5 and vimentin antibodies were purchased from Bioworld Technology, Inc (Minneapolis, USA); Akt, p-Akt; JNK, p-JNK, ERK, p-ERK, p38, p-p38 and GAPDH antibodies were purchased from Kangchen Biotech (Shanghai, China); type I collagen ELISA kits were purchased from Abcam (Cambridge, UK); iScript cDNA synthesis kits and SsoFast EvaGreen Supermix were purchased from Bio-Rad (Hercules, USA); and TRIzol reagent was purchased from TransGen Biotech (Beijing, China). .. Male ICR mice weighing 22±2 g were purchased from the Comparative Medicine Centre of Yangzhou University (Yangzhou, China).

    Purification:

    Article Title: Periscope Proteins are variable length regulators of bacterial cell surface interactions
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    GE Healthcare phenol red free rpmi 1640
    Depletion of androgen increased AR expression and Akt activity LNCaP cells were grown in regular <t>RPMI</t> 1640 medium with 10% FBS and androgen-depleted medium, phenol red-free RPMI 1640 supplemented with 10% charcoal/dextran-treated FBS for 10 days. Cells were lyzed and 50 μg total protein was resolved by electrophoresis on a 10% SDS-PAGE gel. Immunoblotting was performed using AR and phospho-Akt antibodies, respectively. β-actin protein was used as a loading control.
    Phenol Red Free Rpmi 1640, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenol red free rpmi 1640/product/GE Healthcare
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    GE Healthcare rpmi 1640 medium
    Effects of HSC-CM on BM-derived DC differentiation in vitro A. Cell surface marker expression in myeloid cells after HSC-CM treatment measured by flow cytometry. Filled areas are isotype controls; dotted lines are <t>RPMI</t> 1640 medium controls; full lines are HSC-CM. B. Gating strategy of DCs, total MDSCs and subsets. C. The effect of HSC-CM on DCs, MDSCs and MDSC subgroups. Number is percent of the cell population represented by immature DCs (top panel), MDSCs (middle panel) or Mo-MDSCs, G-MDSCs (bottom panel). Percent G-MDSCs was calculated as follows: corrected G-MDSC percent = 100% × CD11b + percent × Ly6G + Ly6C low percent. Corrected Mo-MDSC percent = 100% × CD11b + percent × Ly6G − Ly6C high percent. D. HSC-CM induced MDSCs in a concentration-dependent manner. MEF-CM was a control CM. E. Gr-1 + cells inhibited T-cell proliferation. F. iNOS , Arg-1 , and IL-4Rα expression in Gr-1 + cells according to RT-PCR. Data represent 3-5 independent experiments and are expressed as means ± SD; * P
    Rpmi 1640 Medium, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/GE Healthcare
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rpmi 1640 medium - by Bioz Stars, 2021-03
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    Image Search Results


    Depletion of androgen increased AR expression and Akt activity LNCaP cells were grown in regular RPMI 1640 medium with 10% FBS and androgen-depleted medium, phenol red-free RPMI 1640 supplemented with 10% charcoal/dextran-treated FBS for 10 days. Cells were lyzed and 50 μg total protein was resolved by electrophoresis on a 10% SDS-PAGE gel. Immunoblotting was performed using AR and phospho-Akt antibodies, respectively. β-actin protein was used as a loading control.

    Journal: Oncotarget

    Article Title: Reciprocal feedback inhibition of the androgen receptor and PI3K as a novel therapy for castrate-sensitive and -resistant prostate cancer

    doi:

    Figure Lengend Snippet: Depletion of androgen increased AR expression and Akt activity LNCaP cells were grown in regular RPMI 1640 medium with 10% FBS and androgen-depleted medium, phenol red-free RPMI 1640 supplemented with 10% charcoal/dextran-treated FBS for 10 days. Cells were lyzed and 50 μg total protein was resolved by electrophoresis on a 10% SDS-PAGE gel. Immunoblotting was performed using AR and phospho-Akt antibodies, respectively. β-actin protein was used as a loading control.

    Article Snippet: For the androgen-depletion experiments, LNCaP cells were grown in androgen-depleted medium, phenol red-free RPMI 1640 supplemented with 10% charcoal/dextran-treated FBS (HyClone, Logan, UT).

    Techniques: Expressing, Activity Assay, Electrophoresis, SDS Page

    Effects of HSC-CM on BM-derived DC differentiation in vitro A. Cell surface marker expression in myeloid cells after HSC-CM treatment measured by flow cytometry. Filled areas are isotype controls; dotted lines are RPMI 1640 medium controls; full lines are HSC-CM. B. Gating strategy of DCs, total MDSCs and subsets. C. The effect of HSC-CM on DCs, MDSCs and MDSC subgroups. Number is percent of the cell population represented by immature DCs (top panel), MDSCs (middle panel) or Mo-MDSCs, G-MDSCs (bottom panel). Percent G-MDSCs was calculated as follows: corrected G-MDSC percent = 100% × CD11b + percent × Ly6G + Ly6C low percent. Corrected Mo-MDSC percent = 100% × CD11b + percent × Ly6G − Ly6C high percent. D. HSC-CM induced MDSCs in a concentration-dependent manner. MEF-CM was a control CM. E. Gr-1 + cells inhibited T-cell proliferation. F. iNOS , Arg-1 , and IL-4Rα expression in Gr-1 + cells according to RT-PCR. Data represent 3-5 independent experiments and are expressed as means ± SD; * P

    Journal: Oncotarget

    Article Title: Activated hepatic stellate cells promote liver cancer by induction of myeloid-derived suppressor cells through cyclooxygenase-2

    doi: 10.18632/oncotarget.6839

    Figure Lengend Snippet: Effects of HSC-CM on BM-derived DC differentiation in vitro A. Cell surface marker expression in myeloid cells after HSC-CM treatment measured by flow cytometry. Filled areas are isotype controls; dotted lines are RPMI 1640 medium controls; full lines are HSC-CM. B. Gating strategy of DCs, total MDSCs and subsets. C. The effect of HSC-CM on DCs, MDSCs and MDSC subgroups. Number is percent of the cell population represented by immature DCs (top panel), MDSCs (middle panel) or Mo-MDSCs, G-MDSCs (bottom panel). Percent G-MDSCs was calculated as follows: corrected G-MDSC percent = 100% × CD11b + percent × Ly6G + Ly6C low percent. Corrected Mo-MDSC percent = 100% × CD11b + percent × Ly6G − Ly6C high percent. D. HSC-CM induced MDSCs in a concentration-dependent manner. MEF-CM was a control CM. E. Gr-1 + cells inhibited T-cell proliferation. F. iNOS , Arg-1 , and IL-4Rα expression in Gr-1 + cells according to RT-PCR. Data represent 3-5 independent experiments and are expressed as means ± SD; * P

    Article Snippet: The mouse H22 hepatoma cell line was purchased from Shanghai Cell Bank, Chinese Academy of Sciences, and maintained in RPMI 1640 medium (HyClone, Logan, UT), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin, as previously described [ ].

    Techniques: Derivative Assay, In Vitro, Marker, Expressing, Flow Cytometry, Cytometry, Concentration Assay, Reverse Transcription Polymerase Chain Reaction

    The viability of RAW264.7 murine macrophage cells was measured using MTT assays. The cells were treated with serum-free RPMI-1640 containing various concentrations of LWP (0.3–1 mg mL −1 ) for 12 h. The results are presented as a percentage of the control (CK: untreated cells). The data shown are the mean±s.d. of five determinations.

    Journal: Horticulture Research

    Article Title: Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages

    doi: 10.1038/hortres.2016.17

    Figure Lengend Snippet: The viability of RAW264.7 murine macrophage cells was measured using MTT assays. The cells were treated with serum-free RPMI-1640 containing various concentrations of LWP (0.3–1 mg mL −1 ) for 12 h. The results are presented as a percentage of the control (CK: untreated cells). The data shown are the mean±s.d. of five determinations.

    Article Snippet: RPMI-1640 medium, fetal bovine serum and penicillin/streptomycin solution were from Hyclone.

    Techniques: MTT Assay