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GE Healthcare rpmi 1640 medium
Rpmi 1640 Medium, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rpmi 1640 medium/product/GE Healthcare
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rpmi 1640 medium - by Bioz Stars, 2021-03
86/100 stars

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Article Title: Comparison of the synergistic anticancer activity of AlPcS4 photodynamic therapy in combination with different low-dose chemotherapeutic agents on gastric cancer cells
Article Snippet: Then, AlPcS4 was diluted to a range of 1–32 µg/ml following a gradient dilution method in RPMI-1640 medium on the day of use.

Article Title: Experimental Design Considerations for In Vitro Non-Natural Glycan Display via Metabolic Oligosaccharide Engineering
Article Snippet: 470 ml RPMI 1640 medium (Invitrogen, cat. no. 11875-119) 25 ml fetal bovine serum (FBS; HyClone, cat. no. SH30071.03) 5.0 ml 100× penicillin-streptomycin solution (P/S; Sigma, cat. no. P-0781) Store up to one 1 month at 4°C This medium is used for culturing Jurkat cells.

Infection:

Article Title: lncRNA-CD160 decreases the immunity of CD8+ T cells through epigenetic mechanisms in hepatitis B virus infection
Article Snippet: For the 20 HBV infection mice, 10 mice were injected with LV-lncRNA-CD160 and 10 mice were injected with LV-control. .. CD8+ T cells from patients with CHB infection were transfected with LV-lncRNA-CD160 or LV-control or treated with medium alone (RPMI-1640 medium with 10% FBS) and cultured at 37°C with 5% CO2 for 5 days. .. The culture supernatants were then injected into the HBV-infected mice via intraperitoneal injection.

Transfection:

Article Title: lncRNA-CD160 decreases the immunity of CD8+ T cells through epigenetic mechanisms in hepatitis B virus infection
Article Snippet: For the 20 HBV infection mice, 10 mice were injected with LV-lncRNA-CD160 and 10 mice were injected with LV-control. .. CD8+ T cells from patients with CHB infection were transfected with LV-lncRNA-CD160 or LV-control or treated with medium alone (RPMI-1640 medium with 10% FBS) and cultured at 37°C with 5% CO2 for 5 days. .. The culture supernatants were then injected into the HBV-infected mice via intraperitoneal injection.

Cell Culture:

Article Title: lncRNA-CD160 decreases the immunity of CD8+ T cells through epigenetic mechanisms in hepatitis B virus infection
Article Snippet: For the 20 HBV infection mice, 10 mice were injected with LV-lncRNA-CD160 and 10 mice were injected with LV-control. .. CD8+ T cells from patients with CHB infection were transfected with LV-lncRNA-CD160 or LV-control or treated with medium alone (RPMI-1640 medium with 10% FBS) and cultured at 37°C with 5% CO2 for 5 days. .. The culture supernatants were then injected into the HBV-infected mice via intraperitoneal injection.

Article Title: Autophagy inhibition impairs the epithelial-mesenchymal transition and enhances cisplatin sensitivity in nasopharyngeal carcinoma
Article Snippet: .. In brief, the 6–10B and 5–8F cells were cultured at 3×103 /well in triplicate in 96-well plates and allowed to attach for 12 h. The complete RPMI-1640 medium was then removed and replaced with fresh medium containing drugs. ..

Article Title: Natural killer cells inhibit pulmonary metastasis of hepatocellular carcinoma in nude mice
Article Snippet: Subsequently, the NK cells were activated using recombinant human IL-2 (PeproTech, Inc., Rocky Hill, NJ, USA) at a concentration of 2 pg/ml ( ). .. The MHCC-97H cells were cultured in RPMI-1640 medium containing 10% FBS. .. Detection of NK cell receptors and activated NK cell markers Flow cytometry was used to examine the expression of the following cell surface proteins: Cluster of differentiation (CD)56 [phycoerythrin (PE)-labeled mouse anti-human CD56 clone B159; BD Biosciences, San Diego, CA, USA], CD3 [allophycocyanin (APC)-labeled mouse anti-human CD3 clone UCHTI; BD Pharmingen, San Diego, CA, USA], NKG2D [fluorescein isothiocyanate (FITC)-labeled anti-human NKG2D clone 1D11; eBioscience, Inc., San Diego, CA, USA], NKB1 (FITC-labeled anti-human NKB1 clone DX9; BD Pharmingen), perforin (FITC Perforin Reagent set containing FITC-labeled mouse anti-human perforin clone δG9 and FITC-mouse immunoglobulin (Ig)G2bκ clone 27–35; BD Pharmingen) and granzyme (FITC-labeled mouse anti-human granzyme clone GB11; BD Pharmingen).

Apoptosis Assay:

Article Title: Novel peptide screened from a phage display library antagonizes the activity of CC chemokine receptor 9
Article Snippet: .. Apoptosis assay MOLT4 cells (4×105 /well) were transferred to 24-well plates with each well containing 500 µl RPMI-1640 medium without FBS. .. Each well was treated with a distinct concentration of P1 (0, 0.1 and 1.0 nmol/ml) for 12 h, and then treated with DOX (1 µg/ml) for 12 h. The control groups were treated with various concentrations of P1 (0, 0.1 and 1.0 nmol/ml) for 24 h. The cells were washed twice, and subsequently detected using an Annexin V-FITC and propidium iodide (PI) double staining kit (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) for 15 min at 25°C in the dark, according to the manufacturer's protocol.

Incubation:

Article Title: Hydroxysafflor yellow A induces autophagy in human liver cancer cells by regulating Beclin 1 and ERK expression
Article Snippet: Observation of cell morphology Hep-G2 cells (3x104 cells/ml) were seeded into 6-well plates and incubated for 24 h at 37˚C. .. Subsequently, the RPMI-1640 medium was discarded, 2 µM HSYA was added and the plates were incubated for 24 h at 37˚C. .. Cells were photographed in three randomly selected fields under an inverted microscope (CKX41; Olympus Corporation) at x400 magnification.

Viability Assay:

Article Title: Targeted Inhibition of P4HB Promotes Cell Sensitivity to Gemcitabine in Urothelial Carcinoma of the Bladder
Article Snippet: T24 and 5637 cells were treated with BAC for 72 h and GEM for 48 h. .. Cell Viability Assay and Monoclonal Cell Colony Forming Assay T24 and 5637 cells were seeded in 96-well plates with RPMI-1640 medium containing 10% FBS, followed by treatment with BAC and GEM. .. The viability of BC cells was evaluated by the Cell Counting Kit-8 (CCK-8, Abmole, Houston, USA) assay according to the manufacturer’s instructions.

BAC Assay:

Article Title: Targeted Inhibition of P4HB Promotes Cell Sensitivity to Gemcitabine in Urothelial Carcinoma of the Bladder
Article Snippet: T24 and 5637 cells were treated with BAC for 72 h and GEM for 48 h. .. Cell Viability Assay and Monoclonal Cell Colony Forming Assay T24 and 5637 cells were seeded in 96-well plates with RPMI-1640 medium containing 10% FBS, followed by treatment with BAC and GEM. .. The viability of BC cells was evaluated by the Cell Counting Kit-8 (CCK-8, Abmole, Houston, USA) assay according to the manufacturer’s instructions.

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  • 99
    GE Healthcare phenol red free rpmi 1640
    Depletion of androgen increased AR expression and Akt activity LNCaP cells were grown in regular <t>RPMI</t> 1640 medium with 10% FBS and androgen-depleted medium, phenol red-free RPMI 1640 supplemented with 10% charcoal/dextran-treated FBS for 10 days. Cells were lyzed and 50 μg total protein was resolved by electrophoresis on a 10% SDS-PAGE gel. Immunoblotting was performed using AR and phospho-Akt antibodies, respectively. β-actin protein was used as a loading control.
    Phenol Red Free Rpmi 1640, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenol red free rpmi 1640/product/GE Healthcare
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenol red free rpmi 1640 - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    99
    GE Healthcare rpmi 1640 medium
    Effects of HSC-CM on BM-derived DC differentiation in vitro A. Cell surface marker expression in myeloid cells after HSC-CM treatment measured by flow cytometry. Filled areas are isotype controls; dotted lines are <t>RPMI</t> 1640 medium controls; full lines are HSC-CM. B. Gating strategy of DCs, total MDSCs and subsets. C. The effect of HSC-CM on DCs, MDSCs and MDSC subgroups. Number is percent of the cell population represented by immature DCs (top panel), MDSCs (middle panel) or Mo-MDSCs, G-MDSCs (bottom panel). Percent G-MDSCs was calculated as follows: corrected G-MDSC percent = 100% × CD11b + percent × Ly6G + Ly6C low percent. Corrected Mo-MDSC percent = 100% × CD11b + percent × Ly6G − Ly6C high percent. D. HSC-CM induced MDSCs in a concentration-dependent manner. MEF-CM was a control CM. E. Gr-1 + cells inhibited T-cell proliferation. F. iNOS , Arg-1 , and IL-4Rα expression in Gr-1 + cells according to RT-PCR. Data represent 3-5 independent experiments and are expressed as means ± SD; * P
    Rpmi 1640 Medium, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi 1640 medium/product/GE Healthcare
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rpmi 1640 medium - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

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    Depletion of androgen increased AR expression and Akt activity LNCaP cells were grown in regular RPMI 1640 medium with 10% FBS and androgen-depleted medium, phenol red-free RPMI 1640 supplemented with 10% charcoal/dextran-treated FBS for 10 days. Cells were lyzed and 50 μg total protein was resolved by electrophoresis on a 10% SDS-PAGE gel. Immunoblotting was performed using AR and phospho-Akt antibodies, respectively. β-actin protein was used as a loading control.

    Journal: Oncotarget

    Article Title: Reciprocal feedback inhibition of the androgen receptor and PI3K as a novel therapy for castrate-sensitive and -resistant prostate cancer

    doi:

    Figure Lengend Snippet: Depletion of androgen increased AR expression and Akt activity LNCaP cells were grown in regular RPMI 1640 medium with 10% FBS and androgen-depleted medium, phenol red-free RPMI 1640 supplemented with 10% charcoal/dextran-treated FBS for 10 days. Cells were lyzed and 50 μg total protein was resolved by electrophoresis on a 10% SDS-PAGE gel. Immunoblotting was performed using AR and phospho-Akt antibodies, respectively. β-actin protein was used as a loading control.

    Article Snippet: For the androgen-depletion experiments, LNCaP cells were grown in androgen-depleted medium, phenol red-free RPMI 1640 supplemented with 10% charcoal/dextran-treated FBS (HyClone, Logan, UT).

    Techniques: Expressing, Activity Assay, Electrophoresis, SDS Page

    Effects of HSC-CM on BM-derived DC differentiation in vitro A. Cell surface marker expression in myeloid cells after HSC-CM treatment measured by flow cytometry. Filled areas are isotype controls; dotted lines are RPMI 1640 medium controls; full lines are HSC-CM. B. Gating strategy of DCs, total MDSCs and subsets. C. The effect of HSC-CM on DCs, MDSCs and MDSC subgroups. Number is percent of the cell population represented by immature DCs (top panel), MDSCs (middle panel) or Mo-MDSCs, G-MDSCs (bottom panel). Percent G-MDSCs was calculated as follows: corrected G-MDSC percent = 100% × CD11b + percent × Ly6G + Ly6C low percent. Corrected Mo-MDSC percent = 100% × CD11b + percent × Ly6G − Ly6C high percent. D. HSC-CM induced MDSCs in a concentration-dependent manner. MEF-CM was a control CM. E. Gr-1 + cells inhibited T-cell proliferation. F. iNOS , Arg-1 , and IL-4Rα expression in Gr-1 + cells according to RT-PCR. Data represent 3-5 independent experiments and are expressed as means ± SD; * P

    Journal: Oncotarget

    Article Title: Activated hepatic stellate cells promote liver cancer by induction of myeloid-derived suppressor cells through cyclooxygenase-2

    doi: 10.18632/oncotarget.6839

    Figure Lengend Snippet: Effects of HSC-CM on BM-derived DC differentiation in vitro A. Cell surface marker expression in myeloid cells after HSC-CM treatment measured by flow cytometry. Filled areas are isotype controls; dotted lines are RPMI 1640 medium controls; full lines are HSC-CM. B. Gating strategy of DCs, total MDSCs and subsets. C. The effect of HSC-CM on DCs, MDSCs and MDSC subgroups. Number is percent of the cell population represented by immature DCs (top panel), MDSCs (middle panel) or Mo-MDSCs, G-MDSCs (bottom panel). Percent G-MDSCs was calculated as follows: corrected G-MDSC percent = 100% × CD11b + percent × Ly6G + Ly6C low percent. Corrected Mo-MDSC percent = 100% × CD11b + percent × Ly6G − Ly6C high percent. D. HSC-CM induced MDSCs in a concentration-dependent manner. MEF-CM was a control CM. E. Gr-1 + cells inhibited T-cell proliferation. F. iNOS , Arg-1 , and IL-4Rα expression in Gr-1 + cells according to RT-PCR. Data represent 3-5 independent experiments and are expressed as means ± SD; * P

    Article Snippet: The mouse H22 hepatoma cell line was purchased from Shanghai Cell Bank, Chinese Academy of Sciences, and maintained in RPMI 1640 medium (HyClone, Logan, UT), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin, as previously described [ ].

    Techniques: Derivative Assay, In Vitro, Marker, Expressing, Flow Cytometry, Cytometry, Concentration Assay, Reverse Transcription Polymerase Chain Reaction

    The viability of RAW264.7 murine macrophage cells was measured using MTT assays. The cells were treated with serum-free RPMI-1640 containing various concentrations of LWP (0.3–1 mg mL −1 ) for 12 h. The results are presented as a percentage of the control (CK: untreated cells). The data shown are the mean±s.d. of five determinations.

    Journal: Horticulture Research

    Article Title: Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages

    doi: 10.1038/hortres.2016.17

    Figure Lengend Snippet: The viability of RAW264.7 murine macrophage cells was measured using MTT assays. The cells were treated with serum-free RPMI-1640 containing various concentrations of LWP (0.3–1 mg mL −1 ) for 12 h. The results are presented as a percentage of the control (CK: untreated cells). The data shown are the mean±s.d. of five determinations.

    Article Snippet: RPMI-1640 medium, fetal bovine serum and penicillin/streptomycin solution were from Hyclone.

    Techniques: MTT Assay