rpmi 1640 media (GE Healthcare)

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RPMI 1640 media with L glutamine

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Catalog Number:

sh30027.01

Price:

21.81 USD

Size:

500 mL

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Structured Review

GE Healthcare rpmi 1640 media

https://www.bioz.com/result/rpmi 1640 media/product/GE Healthcare
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

rpmi 1640 media - by Bioz Stars, 2021-03

99/100 stars

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In Vitro:

Article Title: RNA interference in Fasciola gigantica: Establishing and optimization of experimental RNAi in the newly excysted juveniles of the fluke
Article Snippet: Species identification of the NEJs for F . gigantica was done by PCR amplification and sequencing of ITS-2 and 28S rDNA markers [ ]. .. In vitro maintenance of the NEJs Three commercially available culture media RPMI-1640 (Hyclone, USA), DMEM (Hyclone, USA) and DME / F12 (1:1) (Hyclone, USA) were tested for supporting the in vitro survival and growth of the juveniles. .. Growth media were supplemented with the final concentrations of foetal bovine serum (10%) (Hyclone, USA) / chicken serum (50%) (Himedia, India), glucose (2%) and HEPES (25 mM) for enhancing their efficacy in extending the survival of the juveniles.

Cell Culture:

Article Title: The effects of black cohosh on the regulation of estrogen receptor (ERα) and progesterone receptor (PR) in breast cancer cells
Article Snippet: Cell culture and treatment with ligands The human breast cancer cell line, T-47D, was obtained from American Type Culture Collection (Manassas, VA, USA). .. These cells were routinely cultured following the same protocol as previous studies in our laboratory., – Cells were incubated at 37°C in an incubator with 5% CO2 in RPMI-1640 media (Hyclone, Logan, UT, USA) and 10% fetal bovine serum (FBS; Hyclone) that contain growth factors and exogenous steroids which assist in cell growth and proliferation. .. Once the cells acquired proper confluency, the medium was changed to a 5% dextran-coated charcoal (DCC)-stripped FBS.

Article Title: Complement inhibitor factor H expressed by breast cancer cells differentiates CD14+ human monocytes into immunosuppressive macrophages
Article Snippet: .. Monocytes were cultured in RPMI 1640 (#SH30027.01; GE Healthcare Life Sciences) for 48 h, and Macrophage-SFM medium (#11500426; Gibco) for 7 days, at density 1x106 /ml. ..

Article Title: Rhizoma Paridis Saponins Suppresses Tumor Growth in a Rat Model of N-Nitrosomethylbenzylamine-Induced Esophageal Cancer by Inhibiting Cyclooxygenases-2 Pathway
Article Snippet: The other human esophageal squamous cell carcinoma cell line KYSE150 was a gift from Dr. Yutaka Shimada, who established this cell line at the Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Japan [ ]. .. Both cell lines were cultured in RPMI-1640 media (HyClone, Logan, UT, USA) supplemented with 10% FCS (Gibco, Grand Island, NY, USA), 100 units/mL penicillin, and 100 μg/mL streptomycin (Thermo Scientific, Rockford, IL, USA) at 37°C in 5% CO2 . .. Cell viability assay Cell viability was measured by the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) dye reduction assay (Sigma-Aldrich, St. Louis, USA).

Article Title: BEX1 acts as a tumor suppressor in acute myeloid leukemia
Article Snippet: Since BEX1 is capable of limiting cell proliferation, colony formation, tumor formation and inducing apoptosis, drugs that enhance BEX1 expression would be beneficial for the treatment of patients with loss of BEX1 expression in FLT-ITD driven AML. .. Cell culture The human AML cell lines, MV4-11, and MOLM-13, were maintained in RPMI-1640 media (Hyclone, Thermo Scientific, Waltham, MA) supplemented with 10% heat-inactivated fetal bovine serum (Life Technologies, Carlsbad, CA) and 1% penicillin and streptomycin. .. The murine hematopoietic cell line Ba/F3 and the myeloid cell line 32D were cultured in the same medium with addition of 10 ng/ml murine interleukin 3 (IL3) as recommended before [ ].

Incubation:

Multiple Displacement Amplification:

Article Title:
Article Snippet: .. MCF-7, MDA-MB-231, BT-474, DU4475, SKBR3, and T47-D BC cells, and HeLa cervical cancer cells were obtained from American Type Culture Collection and routinely grown in phenol red–containing HyClone RPMI 1640 media with 10% (v/v) heat-inactivated FBS (GE Healthcare Life Sciences) and 1% penicillin-streptomycin. ..

Modification:

Article Title: Matriptase Is Involved in ErbB-2-Induced Prostate Cancer Cell Invasion
Article Snippet: Lipofectamine 2000 transfection reagent and penicillin-streptomycin were purchased from Invitrogen (Carlsbad, CA). .. Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium, and RPMI 1640 culture media were obtained from Hyclone (Logan, UT). .. Protein assay kits were from Bio-Rad (Hercules, CA).

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rpmi 1640 media (GE Healthcare)

GE Healthcare rpmi 1640 media

Concentration-dependent effects of BC on ERα levels. Notes: T-47D cells were cultured in <t>RPMI-1640</t> medium supplemented with 10% FBS for 2 days followed by 6 days in media containing 5% DCC-stripped FBS with media changed every 48 hours. On the seventh day, cells were treated with BC for 24 hours at concentrations of 5–100 µM. Cellular protein extracts were prepared followed by protein quantification, SDS-PAGE, and Western blot analysis. The control lane, Cs, represents cells grown in the absence of ligands in media containing 5% DCC-stripped FBS. The relative intensity of ERα protein, as compared to Cs, is displayed as the mean ± SEM. The asterisk indicates significant difference with respect to the control. * p

Rpmi 1640 Media, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rpmi 1640 media/product/GE Healthcare
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

rpmi 1640 media - by Bioz Stars, 2021-03

99/100 stars

Buy from Supplier

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Concentration-dependent effects of BC on ERα levels. Notes: T-47D cells were cultured in RPMI-1640 medium supplemented with 10% FBS for 2 days followed by 6 days in media containing 5% DCC-stripped FBS with media changed every 48 hours. On the seventh day, cells were treated with BC for 24 hours at concentrations of 5–100 µM. Cellular protein extracts were prepared followed by protein quantification, SDS-PAGE, and Western blot analysis. The control lane, Cs, represents cells grown in the absence of ligands in media containing 5% DCC-stripped FBS. The relative intensity of ERα protein, as compared to Cs, is displayed as the mean ± SEM. The asterisk indicates significant difference with respect to the control. * p

Journal: Breast Cancer : Targets and Therapy

Article Title: The effects of black cohosh on the regulation of estrogen receptor (ERα) and progesterone receptor (PR) in breast cancer cells

doi: 10.2147/BCTT.S144865

Figure Lengend Snippet: Concentration-dependent effects of BC on ERα levels. Notes: T-47D cells were cultured in RPMI-1640 medium supplemented with 10% FBS for 2 days followed by 6 days in media containing 5% DCC-stripped FBS with media changed every 48 hours. On the seventh day, cells were treated with BC for 24 hours at concentrations of 5–100 µM. Cellular protein extracts were prepared followed by protein quantification, SDS-PAGE, and Western blot analysis. The control lane, Cs, represents cells grown in the absence of ligands in media containing 5% DCC-stripped FBS. The relative intensity of ERα protein, as compared to Cs, is displayed as the mean ± SEM. The asterisk indicates significant difference with respect to the control. * p

Article Snippet: These cells were routinely cultured following the same protocol as previous studies in our laboratory., – Cells were incubated at 37°C in an incubator with 5% CO2 in RPMI-1640 media (Hyclone, Logan, UT, USA) and 10% fetal bovine serum (FBS; Hyclone) that contain growth factors and exogenous steroids which assist in cell growth and proliferation.

Techniques: Concentration Assay, Cell Culture, Droplet Countercurrent Chromatography, SDS Page, Western Blot

Concentration-dependent effects of BC on PR-A/B levels. Notes: T-47D cells were cultured in RPMI-1640 medium supplemented with 10% FBS for 2 days followed by 6 days in media containing 5% DCC-stripped FBS with media changed every 48 hours. On the seventh day, cells were treated with BC for 24 hours at concentrations of 5–100 µM. Cellular protein extracts were prepared followed by protein quantification, SDS-PAGE, and Western blot analysis. The control lane, Cs, represents cells grown in the absence of ligands in media containing 5% DCC-stripped FBS. The relative intensity of PR-A/B protein, as compared to Cs, is displayed as the mean ± SEM. The asterisk indicates significant difference with respect to the control. *** p

Journal: Breast Cancer : Targets and Therapy

Article Title: The effects of black cohosh on the regulation of estrogen receptor (ERα) and progesterone receptor (PR) in breast cancer cells

doi: 10.2147/BCTT.S144865

Figure Lengend Snippet: Concentration-dependent effects of BC on PR-A/B levels. Notes: T-47D cells were cultured in RPMI-1640 medium supplemented with 10% FBS for 2 days followed by 6 days in media containing 5% DCC-stripped FBS with media changed every 48 hours. On the seventh day, cells were treated with BC for 24 hours at concentrations of 5–100 µM. Cellular protein extracts were prepared followed by protein quantification, SDS-PAGE, and Western blot analysis. The control lane, Cs, represents cells grown in the absence of ligands in media containing 5% DCC-stripped FBS. The relative intensity of PR-A/B protein, as compared to Cs, is displayed as the mean ± SEM. The asterisk indicates significant difference with respect to the control. *** p

Techniques: Concentration Assay, Cell Culture, Droplet Countercurrent Chromatography, SDS Page, Western Blot

Cell growth and phosphorylation status of AKT in acid-tolerable H-2452AcT cells. (A, B) H-2452 and H-2452AcT cells were incubated with the RPMI-1640 medium containing (a) or not containing (b) 3.8 μM of lactic acid for 24 h, 48 h, and 72 h. The cell viability and p-AKT level were determined using an MTT assay and a western-blot analysis, respectively. (C) Cells were incubated with the RPMI-1640 medium without lactic acid for 24 h, 48 h, and 72 h. The cell distributions in the sub-G 0 /G 1 , G 0 /G 1 , S, and G 2 /M phases were analyzed using flow cytometry following a propidium-iodide staining (20 μg/ml). The error bars indicate the mean ± standard deviation for three independent experiments. The β-actin was used as a loading control. * P

Journal: Molecules and Cells

Article Title: Cariporide Enhances the DNA Damage and Apoptosis in Acid-tolerable Malignant Mesothelioma H-2452 Cells

doi: 10.14348/molcells.2017.0059

Figure Lengend Snippet: Cell growth and phosphorylation status of AKT in acid-tolerable H-2452AcT cells. (A, B) H-2452 and H-2452AcT cells were incubated with the RPMI-1640 medium containing (a) or not containing (b) 3.8 μM of lactic acid for 24 h, 48 h, and 72 h. The cell viability and p-AKT level were determined using an MTT assay and a western-blot analysis, respectively. (C) Cells were incubated with the RPMI-1640 medium without lactic acid for 24 h, 48 h, and 72 h. The cell distributions in the sub-G 0 /G 1 , G 0 /G 1 , S, and G 2 /M phases were analyzed using flow cytometry following a propidium-iodide staining (20 μg/ml). The error bars indicate the mean ± standard deviation for three independent experiments. The β-actin was used as a loading control. * P

Article Snippet: The H-2452 cells were maintained at 37°C in an RPMI-1640 medium (SH30027.01; GE Healthcare Life Sci., Australia) supplemented with 10% fetal bovine serum (SH30084.03; GE Healthcare Life Sci.), 100 U/ml penicillin, and 100 μg/ml streptomycin.

Techniques: Incubation, MTT Assay, Western Blot, Flow Cytometry, Cytometry, Staining, Standard Deviation

FH changes the morphology and prolongs the viability of monocytes. (a) Morphological changes in human peripheral blood CD14 + monocytes upon incubation for 48 h with RPMI 1640 medium, 150 μg/ml FH, α1-AT or heat-denatured FH. (b) Cell death assessed by Sytox green inclusion of monocytes incubated with medium, 150 μg/ml FH or α1-AT for 8 days. (c) Morphology of mouse bone marrow progenitor cells incubated with medium or 150 μg/ml FH for 6 days. (d–i) Scanning electron microscopy of monocytes incubated with medium (d–f) or 150 μg/ml FH (G-I) for 6 days. (j–n) Quantification of cell circularity (j), elongation (k), cell area (l), cellular vesicles (m) and cell-cell contact (n) of CD14 + monocytes cultured for 2 and 6 days in medium, 150 μg/ml FH or α1-AT. Data are means ± SD of n=500 cells.

Journal: Oncoimmunology

Article Title: Complement inhibitor factor H expressed by breast cancer cells differentiates CD14+ human monocytes into immunosuppressive macrophages

doi: 10.1080/2162402X.2020.1731135

Figure Lengend Snippet: FH changes the morphology and prolongs the viability of monocytes. (a) Morphological changes in human peripheral blood CD14 + monocytes upon incubation for 48 h with RPMI 1640 medium, 150 μg/ml FH, α1-AT or heat-denatured FH. (b) Cell death assessed by Sytox green inclusion of monocytes incubated with medium, 150 μg/ml FH or α1-AT for 8 days. (c) Morphology of mouse bone marrow progenitor cells incubated with medium or 150 μg/ml FH for 6 days. (d–i) Scanning electron microscopy of monocytes incubated with medium (d–f) or 150 μg/ml FH (G-I) for 6 days. (j–n) Quantification of cell circularity (j), elongation (k), cell area (l), cellular vesicles (m) and cell-cell contact (n) of CD14 + monocytes cultured for 2 and 6 days in medium, 150 μg/ml FH or α1-AT. Data are means ± SD of n=500 cells.

Article Snippet: Monocytes were cultured in RPMI 1640 (#SH30027.01; GE Healthcare Life Sciences) for 48 h, and Macrophage-SFM medium (#11500426; Gibco) for 7 days, at density 1x106 /ml.

Techniques: Incubation, Electron Microscopy, Cell Culture

Survival of F . gigantica NEJs in RPMI-1640, DMEM and DME/F-12 culture media supplemented with 10% foetal bovine serum, 2% glucose and 25 mM HEPES. NEJs showed higher % survival (≥ 80%) in RPMI-1640 which was lower in DMEM and DME/F-12 medium (≥75% and ≥72%), respectively at 3 weeks of culture. All flukes died by 28–30 days of culture.

Journal: PLoS Neglected Tropical Diseases

Article Title: RNA interference in Fasciola gigantica: Establishing and optimization of experimental RNAi in the newly excysted juveniles of the fluke

doi: 10.1371/journal.pntd.0006109

Figure Lengend Snippet: Survival of F . gigantica NEJs in RPMI-1640, DMEM and DME/F-12 culture media supplemented with 10% foetal bovine serum, 2% glucose and 25 mM HEPES. NEJs showed higher % survival (≥ 80%) in RPMI-1640 which was lower in DMEM and DME/F-12 medium (≥75% and ≥72%), respectively at 3 weeks of culture. All flukes died by 28–30 days of culture.

Article Snippet: In vitro maintenance of the NEJs Three commercially available culture media RPMI-1640 (Hyclone, USA), DMEM (Hyclone, USA) and DME / F12 (1:1) (Hyclone, USA) were tested for supporting the in vitro survival and growth of the juveniles.

Techniques: