rotor gene q real time pcr cycler  (Qiagen)


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    Name:
    Rotor Gene Q MDx System
    Description:
    For in vitro diagnostic use with FDA cleared or approved nucleic acid tests in clinical laboratories Kit contents Real time PCR cycler with 6 channels the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests laptop computer software accessories 1 year warranty on parts and labor installation and training Benefits Optimal thermal and optical performance due to rotary format Robust technology requiring minimal maintenance Easy to use software with advanced security features
    Catalog Number:
    9002036
    Price:
    Inquire
    Category:
    Rotor Gene Q MDx US
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    Structured Review

    Qiagen rotor gene q real time pcr cycler
    Rotor Gene Q MDx System
    For in vitro diagnostic use with FDA cleared or approved nucleic acid tests in clinical laboratories Kit contents Real time PCR cycler with 6 channels the red and HRM channels are not intended for use with FDA cleared or approved nucleic acid tests laptop computer software accessories 1 year warranty on parts and labor installation and training Benefits Optimal thermal and optical performance due to rotary format Robust technology requiring minimal maintenance Easy to use software with advanced security features
    https://www.bioz.com/result/rotor gene q real time pcr cycler/product/Qiagen
    Average 90 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    rotor gene q real time pcr cycler - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: Clinical performance of an analytically validated assay in comparison to microarray technology to assess PITX2 DNA-methylation in breast cancer
    Article Snippet: The PITX2 qMSP assay was optimized for the usage as an in vitro diagnostic assay following the CLSI guidelines ( https://clsi.org/ ; see also ). .. PCR analysis is performed on Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, Hilden, Germany).

    Article Title: New Drugs/Drug News
    Article Snippet: Source: ; The Wall Street Journal , May 1, 2012 Name: Rotor-Gene Q MDx Manufacturer: Qiagen, The Netherlands Approval Date: April 17, 2012 Purpose: This automated molecular detection platform is based on real-time polymerase chain reaction (PCR) technology. .. The system is intended for in vitro diagnostic use with FDA-approved nucleic acid tests, such as those used for hepatitis B and C and HIV infections, in clinical laboratories.

    Clone Assay:

    Article Title: Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat
    Article Snippet: Paragraph title: cDNA cloning from R. pirica tadpoles and quantitative real-time PCR ... Quantitative real time PCR was performed using Rotor Gene Q MDx 5plex HRM (QIAGEN).

    Amplification:

    Article Title: Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat
    Article Snippet: The amplification procedure was performed according to the manufacturer’s protocol. .. Quantitative real time PCR was performed using Rotor Gene Q MDx 5plex HRM (QIAGEN).

    Article Title: Methylenetetrahydrofolate reductase gene C677T and A1298C polymorphisms and susceptibility to recurrent pregnancy loss
    Article Snippet: For all subjects, molecular testing was performed on venous blood samples (in EDTA-K3) according to the following steps: i) DNA was isolated from 25 µl venous blood using a DNA extraction kit obtained from Nuclear Laser Medicine Srl (Settala, Italy; cat. no. AA1001); ii) to study the C677T and A1298C variants of the MTHFR gene, the target genomic area was amplified by polymerase chain reaction (PCR) using two commercial kits provided by Nuclear Laser Medicine Srl [cat. nos. .. Following kit protocols, this enabled the detection of single nucleotide polymorphisms using a Rotor-Gene Q MDx (Qiagen Srl, Milan, Italy) using fluorescent oligos annealed to specific DNA target sequences in the gene of interest, and the PCR products were quantified using the Fluorescent Resonance Energy Transfer method ( ); iii) the variants of the target sequences were measured from melting curves.

    Article Title: Clinical performance of an analytically validated assay in comparison to microarray technology to assess PITX2 DNA-methylation in breast cancer
    Article Snippet: Validity criteria for run controls and sample testing include cycle threshold (Ct), amplification curve anomalies, PMR range for the controls (PITX2 RGQ PCR reference 50, PITX2 RGQ PCR reference low, PITX2 RGQ PCR negative control (NC), PITX2 RGQ PCR NTC (NTC)) and delta-PMR threshold for sample duplicates as described in detail in the handbook . .. PCR analysis is performed on Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, Hilden, Germany).

    Article Title: Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice
    Article Snippet: Paragraph title: HRM-PCR Amplification and Data Analysis ... SSR-HRM analysis was performed using a Rotor-Gene Q MDx (QIAGEN) real-time PCR machine.

    Article Title: Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species
    Article Snippet: .. DNA amplification was performed in a Rotor-Gene Q MDx (QIAGEN) real-time PCR under the following conditions: 94 °C for 3 min, followed by 40 cycles of 94 °C for 30 s, 56 °C for 30 s and 72 °C for 45 s. The fluorescent data were acquired at the end of each extension step during the PCR cycles. .. HRM analysis was performed immediately following the completion of PCR amplification.

    Article Title: Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice
    Article Snippet: .. HRM-PCR Amplification and Data Analysis SSR-HRM analysis was performed using a Rotor-Gene Q MDx (QIAGEN) real-time PCR machine. .. The HRM reaction mixture contained 50 ng of genomic DNA, 5 μL of 2× HRM master mix (QIAGEN, Germany), 0.5 μL of 10 μmol/L of both forward and reverse primers, and distilled water was added to reach the final volume.

    Mass Spectrometry:

    Article Title: New Drugs/Drug News
    Article Snippet: Paragraph title: Marvin M. Goldenberg, PhD, RPh, MS ... Source: ; The Wall Street Journal , May 1, 2012 Name: Rotor-Gene Q MDx Manufacturer: Qiagen, The Netherlands Approval Date: April 17, 2012 Purpose: This automated molecular detection platform is based on real-time polymerase chain reaction (PCR) technology.

    Quantitative RT-PCR:

    Article Title: Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation
    Article Snippet: Paragraph title: 2.4. Real-Time RT-qPCR Analysis ... The QuantiFast SYBR® Green PCR Kit (Qiagen, Hilden, Germany) was used in the Rotor-Gene Q MDx 5plex Platform (Qiagen, Hilden, Germany), according to a previously published protocol (Al-Zghoul et al., 2019).

    SYBR Green Assay:

    Article Title: Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat
    Article Snippet: Quantitative real time PCR was performed using Rotor Gene Q MDx 5plex HRM (QIAGEN). .. In the qPCR, 18S ribosomal RNA obtained from R. pirica was used as the internal control. qPCR was performed using Rotor-Gene SYBR® Green PCR Kit (QIAGEN) as described in the manufacturer’s protocol.

    Article Title: Molecular Signature of Indeterminate Thyroid Lesions: Current Methods to Improve Fine Needle Aspiration Cytology (FNAC) Diagnosis
    Article Snippet: Two µL of cDNA was used as template for real time PCR (RT-PCR) to measure miRNA expression levels with the miScript SYBR Green PCR Kit (Qiagen) with specific primers for miRNA-95 and -190 (Qiagen). .. RT-PCR was performed in duplicate on the Rotor-gene Q MDx (Qiagen) under the following cycling conditions: 95 °C for 15 min, 40 cycles at 94 °C for 15 s, 55 °C for 30 s and 70 °C for 30 s. Relative expression levels were calculated using the 2−ΔΔC t method with the miRNA-16 as endogenous control. miRNA expression correctly identified 38/43 (specificity of 88.4%) of the benign lesions and 27/32 (sensitivity of 84.3%) of malignant sample.

    Article Title: Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation
    Article Snippet: .. The QuantiFast SYBR® Green PCR Kit (Qiagen, Hilden, Germany) was used in the Rotor-Gene Q MDx 5plex Platform (Qiagen, Hilden, Germany), according to a previously published protocol (Al-Zghoul et al., 2019). .. Triplicates from each cDNA library were analyzed. shows the sequences of the primers that were used in the real-time RT-PCR analysis.

    Article Title: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
    Article Snippet: .. For nDKO and control samples, RT-PCR was completed using QuantiTect® SYBR® Green RT-PCR Kit in Qiagen Rotor-Gene Q MDx (Qiagen Inc.) and 0.5 ng of mRNA. ..

    Incubation:

    Article Title: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
    Article Snippet: Resuspension Media [0.05 mg/mL DNase1 (Roche), 10% fetal bovine serum (Wisent Bioproducts) in DMEM:F12 phenol-free medium] was added to each tube and incubated for five minutes. .. For nDKO and control samples, RT-PCR was completed using QuantiTect® SYBR® Green RT-PCR Kit in Qiagen Rotor-Gene Q MDx (Qiagen Inc.) and 0.5 ng of mRNA.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Epigenetic IVD Tests for Personalized Precision Medicine in Cancer
    Article Snippet: The Therascreen PITX2 RGQ PCR assay , developed by in conjunction with the Therawis Diagnostics Company, analyzes the methylation status of the PITX2 gene obtained from the DNA of FFPE biospecimens. .. It runs in the real-time Rotor-Gene Q MDx thermal cycler (Qiagen) or a Rotor-Gene Q MDx 5plex HRM instrument (Qiagen).

    Infection:

    Article Title: New Drugs/Drug News
    Article Snippet: Associated risks include device failure, clinician error, damage to the abdominal cavity, infection, and tissue inflammation. .. Source: ; The Wall Street Journal , May 1, 2012 Name: Rotor-Gene Q MDx Manufacturer: Qiagen, The Netherlands Approval Date: April 17, 2012 Purpose: This automated molecular detection platform is based on real-time polymerase chain reaction (PCR) technology.

    Expressing:

    Article Title: Molecular Signature of Indeterminate Thyroid Lesions: Current Methods to Improve Fine Needle Aspiration Cytology (FNAC) Diagnosis
    Article Snippet: .. RT-PCR was performed in duplicate on the Rotor-gene Q MDx (Qiagen) under the following cycling conditions: 95 °C for 15 min, 40 cycles at 94 °C for 15 s, 55 °C for 30 s and 70 °C for 30 s. Relative expression levels were calculated using the 2−ΔΔC t method with the miRNA-16 as endogenous control. miRNA expression correctly identified 38/43 (specificity of 88.4%) of the benign lesions and 27/32 (sensitivity of 84.3%) of malignant sample. ..

    Förster Resonance Energy Transfer:

    Article Title: Methylenetetrahydrofolate reductase gene C677T and A1298C polymorphisms and susceptibility to recurrent pregnancy loss
    Article Snippet: .. Following kit protocols, this enabled the detection of single nucleotide polymorphisms using a Rotor-Gene Q MDx (Qiagen Srl, Milan, Italy) using fluorescent oligos annealed to specific DNA target sequences in the gene of interest, and the PCR products were quantified using the Fluorescent Resonance Energy Transfer method ( ); iii) the variants of the target sequences were measured from melting curves. .. The results were compared through receiver operating characteristic (ROC) curve using JMP software version 2 ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Molecular Signature of Indeterminate Thyroid Lesions: Current Methods to Improve Fine Needle Aspiration Cytology (FNAC) Diagnosis
    Article Snippet: .. RT-PCR was performed in duplicate on the Rotor-gene Q MDx (Qiagen) under the following cycling conditions: 95 °C for 15 min, 40 cycles at 94 °C for 15 s, 55 °C for 30 s and 70 °C for 30 s. Relative expression levels were calculated using the 2−ΔΔC t method with the miRNA-16 as endogenous control. miRNA expression correctly identified 38/43 (specificity of 88.4%) of the benign lesions and 27/32 (sensitivity of 84.3%) of malignant sample. ..

    Article Title: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
    Article Snippet: .. For nDKO and control samples, RT-PCR was completed using QuantiTect® SYBR® Green RT-PCR Kit in Qiagen Rotor-Gene Q MDx (Qiagen Inc.) and 0.5 ng of mRNA. ..

    other:

    Article Title: Evaluation of six TaqMan RT-rtPCR kits on two thermocyclers for the reliable detection of rabies virus RNA
    Article Snippet: The analytical sensitivity of 18.1–25.8 copies/µL for Rotor-Gene Q MDx and 16.7–21.5 copies/µL for Mx3005P were acceptable.

    Sequencing:

    Article Title: Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat
    Article Snippet: Each cDNA fragment was cloned into pCRII-TOPO (Invitrogen), and sequencing was carried out using BigDye terminator version 3.1 and an ABI 3100 DNA sequencer (Applied Biosystems). .. Quantitative real time PCR was performed using Rotor Gene Q MDx 5plex HRM (QIAGEN).

    DNA Extraction:

    Article Title: Methylenetetrahydrofolate reductase gene C677T and A1298C polymorphisms and susceptibility to recurrent pregnancy loss
    Article Snippet: Paragraph title: DNA extraction and molecular analysis ... Following kit protocols, this enabled the detection of single nucleotide polymorphisms using a Rotor-Gene Q MDx (Qiagen Srl, Milan, Italy) using fluorescent oligos annealed to specific DNA target sequences in the gene of interest, and the PCR products were quantified using the Fluorescent Resonance Energy Transfer method ( ); iii) the variants of the target sequences were measured from melting curves.

    Fluorescence:

    Article Title: Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice
    Article Snippet: SSR-HRM analysis was performed using a Rotor-Gene Q MDx (QIAGEN) real-time PCR machine. .. HRM-PCR was conducted in a 72-well carousel with the following reaction conditions: an initial denaturing step of 94°C for 3 min, followed by 40 cycles of 94°C for 30 s, primer annealing temperature for 30 s, and 72°C for 30 s. Fluorescence data was collected at the end of each extension step of each PCR cycle.

    Article Title: Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species
    Article Snippet: DNA amplification was performed in a Rotor-Gene Q MDx (QIAGEN) real-time PCR under the following conditions: 94 °C for 3 min, followed by 40 cycles of 94 °C for 30 s, 56 °C for 30 s and 72 °C for 45 s. The fluorescent data were acquired at the end of each extension step during the PCR cycles. .. Fluorescence was continuously monitored during the slow warming up of the gradient curve with a sharp fluorescence drop near the denaturation temperature.

    Article Title: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
    Article Snippet: Paragraph title: Fluorescence-Activated Cell Sorting (FACS) Analysis and PCR ... For nDKO and control samples, RT-PCR was completed using QuantiTect® SYBR® Green RT-PCR Kit in Qiagen Rotor-Gene Q MDx (Qiagen Inc.) and 0.5 ng of mRNA.

    Article Title: Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice
    Article Snippet: HRM-PCR Amplification and Data Analysis SSR-HRM analysis was performed using a Rotor-Gene Q MDx (QIAGEN) real-time PCR machine. .. HRM-PCR was conducted in a 72-well carousel with the following reaction conditions: an initial denaturing step of 94°C for 3 min, followed by 40 cycles of 94°C for 30 s, primer annealing temperature for 30 s, and 72°C for 30 s. Fluorescence data was collected at the end of each extension step of each PCR cycle.

    Methylation:

    Article Title: Clinical performance of an analytically validated assay in comparison to microarray technology to assess PITX2 DNA-methylation in breast cancer
    Article Snippet: The PMR (percent methylation ratio) is calculated applying the following formula: PMR = 100/(1 + 2exp(CtFAM(methylated) − CtHEX(unmethylated) )] . .. PCR analysis is performed on Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, Hilden, Germany).

    Article Title: Epigenetic IVD Tests for Personalized Precision Medicine in Cancer
    Article Snippet: PITX2 methylation is assessed by methylation-specific real-time PCR and exploits the quantitative PCR (qPCR) oligonucleotide hydrolysis principle of two TaqMan probes labeled with different fluorescent dies (FAM™ for fully methylated and HEX™ for fully unmethylated DNA) in combination with methylation nonspecific primers to measure the methylation status of the target sequences of PITX2 gene promoter 2 in bisulfite-treated DNA. .. It runs in the real-time Rotor-Gene Q MDx thermal cycler (Qiagen) or a Rotor-Gene Q MDx 5plex HRM instrument (Qiagen).

    Isolation:

    Article Title: Methylenetetrahydrofolate reductase gene C677T and A1298C polymorphisms and susceptibility to recurrent pregnancy loss
    Article Snippet: For all subjects, molecular testing was performed on venous blood samples (in EDTA-K3) according to the following steps: i) DNA was isolated from 25 µl venous blood using a DNA extraction kit obtained from Nuclear Laser Medicine Srl (Settala, Italy; cat. no. AA1001); ii) to study the C677T and A1298C variants of the MTHFR gene, the target genomic area was amplified by polymerase chain reaction (PCR) using two commercial kits provided by Nuclear Laser Medicine Srl [cat. nos. .. Following kit protocols, this enabled the detection of single nucleotide polymorphisms using a Rotor-Gene Q MDx (Qiagen Srl, Milan, Italy) using fluorescent oligos annealed to specific DNA target sequences in the gene of interest, and the PCR products were quantified using the Fluorescent Resonance Energy Transfer method ( ); iii) the variants of the target sequences were measured from melting curves.

    Article Title: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
    Article Snippet: Isolated cells were sorted with a Beckman MoFlo AstriosEQ (Beckman Coulter Canada, Mississauga, ON, Canada) for GFP (for Nestin GFP mice) or YFP (for nDKO and control mice) using the University of Ottawa FACS Core Facility. mRNA was extracted using Arcturus Picopure RNA Isolation Kit (Applied Biosystems, ThermoFisher). .. For nDKO and control samples, RT-PCR was completed using QuantiTect® SYBR® Green RT-PCR Kit in Qiagen Rotor-Gene Q MDx (Qiagen Inc.) and 0.5 ng of mRNA.

    Negative Control:

    Article Title: Clinical performance of an analytically validated assay in comparison to microarray technology to assess PITX2 DNA-methylation in breast cancer
    Article Snippet: Validity criteria for run controls and sample testing include cycle threshold (Ct), amplification curve anomalies, PMR range for the controls (PITX2 RGQ PCR reference 50, PITX2 RGQ PCR reference low, PITX2 RGQ PCR negative control (NC), PITX2 RGQ PCR NTC (NTC)) and delta-PMR threshold for sample duplicates as described in detail in the handbook . .. PCR analysis is performed on Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, Hilden, Germany).

    Labeling:

    Article Title: Epigenetic IVD Tests for Personalized Precision Medicine in Cancer
    Article Snippet: PITX2 methylation is assessed by methylation-specific real-time PCR and exploits the quantitative PCR (qPCR) oligonucleotide hydrolysis principle of two TaqMan probes labeled with different fluorescent dies (FAM™ for fully methylated and HEX™ for fully unmethylated DNA) in combination with methylation nonspecific primers to measure the methylation status of the target sequences of PITX2 gene promoter 2 in bisulfite-treated DNA. .. It runs in the real-time Rotor-Gene Q MDx thermal cycler (Qiagen) or a Rotor-Gene Q MDx 5plex HRM instrument (Qiagen).

    Purification:

    Article Title: Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat
    Article Snippet: Mixed total RNA (1 μg) purified from the tail parts of tadpoles exposed to different predation threats were used for cDNA synthesis using 10 pmol of anchored T17ADP primer (5′-GAG TCG ACT CGA GAA TTC T17 -3′) and Superscript™III (Invitrogen). .. Quantitative real time PCR was performed using Rotor Gene Q MDx 5plex HRM (QIAGEN).

    Polymerase Chain Reaction:

    Article Title: Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat
    Article Snippet: The selected cDNA fragments were produced by PCR using gene specific primers and KOD FX Neo (TOYOBO). .. Quantitative real time PCR was performed using Rotor Gene Q MDx 5plex HRM (QIAGEN).

    Article Title: Methylenetetrahydrofolate reductase gene C677T and A1298C polymorphisms and susceptibility to recurrent pregnancy loss
    Article Snippet: .. Following kit protocols, this enabled the detection of single nucleotide polymorphisms using a Rotor-Gene Q MDx (Qiagen Srl, Milan, Italy) using fluorescent oligos annealed to specific DNA target sequences in the gene of interest, and the PCR products were quantified using the Fluorescent Resonance Energy Transfer method ( ); iii) the variants of the target sequences were measured from melting curves. .. The results were compared through receiver operating characteristic (ROC) curve using JMP software version 2 ( ).

    Article Title: Surgical and Pathological Changes after Radiofrequency Ablation of Thyroid Nodules
    Article Snippet: BRAF mutational status (including V600E/Ec, V600D, 600K, and V600R mutations) was evaluated by Therascreen BRAF RSQ PCR kit (Qiagen, UK). .. DNA was extracted from paraffin blocks and tested by using real-time polymerase chain reaction on the Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, UK) and on the PyroMark Q24 System (Qiagen, UK), respectively.

    Article Title: Evaluation of six TaqMan RT-rtPCR kits on two thermocyclers for the reliable detection of rabies virus RNA
    Article Snippet: The same PCR thermal cycling conditions ( ) were applied for the 6 kits tested (i.e., 45 cycles of 30 s at 94°C, 30 s at 55°C, and 30 s at 72°C). .. Real-time PCRs were performed on 2 real-time thermocyclers: Rotor-Gene Q MDx (RG, Qiagen) and Mx3005P (Agilent Technologies, Les Ulis, France).

    Article Title: Molecular Signature of Indeterminate Thyroid Lesions: Current Methods to Improve Fine Needle Aspiration Cytology (FNAC) Diagnosis
    Article Snippet: Two µL of cDNA was used as template for real time PCR (RT-PCR) to measure miRNA expression levels with the miScript SYBR Green PCR Kit (Qiagen) with specific primers for miRNA-95 and -190 (Qiagen). .. RT-PCR was performed in duplicate on the Rotor-gene Q MDx (Qiagen) under the following cycling conditions: 95 °C for 15 min, 40 cycles at 94 °C for 15 s, 55 °C for 30 s and 70 °C for 30 s. Relative expression levels were calculated using the 2−ΔΔC t method with the miRNA-16 as endogenous control. miRNA expression correctly identified 38/43 (specificity of 88.4%) of the benign lesions and 27/32 (sensitivity of 84.3%) of malignant sample.

    Article Title: Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation
    Article Snippet: .. The QuantiFast SYBR® Green PCR Kit (Qiagen, Hilden, Germany) was used in the Rotor-Gene Q MDx 5plex Platform (Qiagen, Hilden, Germany), according to a previously published protocol (Al-Zghoul et al., 2019). .. Triplicates from each cDNA library were analyzed. shows the sequences of the primers that were used in the real-time RT-PCR analysis.

    Article Title: Clinical performance of an analytically validated assay in comparison to microarray technology to assess PITX2 DNA-methylation in breast cancer
    Article Snippet: .. PCR analysis is performed on Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, Hilden, Germany). .. Patients and tumor samples For the analytical and technical evaluation of the therascreen PITX2 RGQ PCR assay, Formalin-Fixed Paraffin-Embedded (FFPE) tumor tissue samples from invasive breast cancers (n = 131; thereof 61 ER+ and 68 ER−) were collected at the Institute of Pathology, Technische Universität München.

    Article Title: New Drugs/Drug News
    Article Snippet: .. Source: ; The Wall Street Journal , May 1, 2012 Name: Rotor-Gene Q MDx Manufacturer: Qiagen, The Netherlands Approval Date: April 17, 2012 Purpose: This automated molecular detection platform is based on real-time polymerase chain reaction (PCR) technology. .. The system is intended for in vitro diagnostic use with FDA-approved nucleic acid tests, such as those used for hepatitis B and C and HIV infections, in clinical laboratories.

    Article Title: Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice
    Article Snippet: SSR-HRM analysis was performed using a Rotor-Gene Q MDx (QIAGEN) real-time PCR machine. .. HRM-PCR was conducted in a 72-well carousel with the following reaction conditions: an initial denaturing step of 94°C for 3 min, followed by 40 cycles of 94°C for 30 s, primer annealing temperature for 30 s, and 72°C for 30 s. Fluorescence data was collected at the end of each extension step of each PCR cycle.

    Article Title: Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species
    Article Snippet: .. DNA amplification was performed in a Rotor-Gene Q MDx (QIAGEN) real-time PCR under the following conditions: 94 °C for 3 min, followed by 40 cycles of 94 °C for 30 s, 56 °C for 30 s and 72 °C for 45 s. The fluorescent data were acquired at the end of each extension step during the PCR cycles. .. HRM analysis was performed immediately following the completion of PCR amplification.

    Article Title: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
    Article Snippet: Paragraph title: Fluorescence-Activated Cell Sorting (FACS) Analysis and PCR ... For nDKO and control samples, RT-PCR was completed using QuantiTect® SYBR® Green RT-PCR Kit in Qiagen Rotor-Gene Q MDx (Qiagen Inc.) and 0.5 ng of mRNA.

    Article Title: Epigenetic IVD Tests for Personalized Precision Medicine in Cancer
    Article Snippet: Paragraph title: The Therascreen PITX2 RGQ PCR Kit ... It runs in the real-time Rotor-Gene Q MDx thermal cycler (Qiagen) or a Rotor-Gene Q MDx 5plex HRM instrument (Qiagen).

    Article Title: Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice
    Article Snippet: HRM-PCR Amplification and Data Analysis SSR-HRM analysis was performed using a Rotor-Gene Q MDx (QIAGEN) real-time PCR machine. .. HRM-PCR was conducted in a 72-well carousel with the following reaction conditions: an initial denaturing step of 94°C for 3 min, followed by 40 cycles of 94°C for 30 s, primer annealing temperature for 30 s, and 72°C for 30 s. Fluorescence data was collected at the end of each extension step of each PCR cycle.

    Staining:

    Article Title: Surgical and Pathological Changes after Radiofrequency Ablation of Thyroid Nodules
    Article Snippet: In one case HBME-1 staining, which is considered a good marker of malignancy [ , ], was also performed and evaluated. .. DNA was extracted from paraffin blocks and tested by using real-time polymerase chain reaction on the Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, UK) and on the PyroMark Q24 System (Qiagen, UK), respectively.

    cDNA Library Assay:

    Article Title: Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation
    Article Snippet: The QuantiFast SYBR® Green PCR Kit (Qiagen, Hilden, Germany) was used in the Rotor-Gene Q MDx 5plex Platform (Qiagen, Hilden, Germany), according to a previously published protocol (Al-Zghoul et al., 2019). .. Triplicates from each cDNA library were analyzed. shows the sequences of the primers that were used in the real-time RT-PCR analysis.

    Activated Clotting Time Assay:

    Article Title: Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat
    Article Snippet: Mixed total RNA (1 μg) purified from the tail parts of tadpoles exposed to different predation threats were used for cDNA synthesis using 10 pmol of anchored T17ADP primer (5′-GAG TCG ACT CGA GAA TTC T17 -3′) and Superscript™III (Invitrogen). .. Quantitative real time PCR was performed using Rotor Gene Q MDx 5plex HRM (QIAGEN).

    Mouse Assay:

    Article Title: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
    Article Snippet: Isolated cells were sorted with a Beckman MoFlo AstriosEQ (Beckman Coulter Canada, Mississauga, ON, Canada) for GFP (for Nestin GFP mice) or YFP (for nDKO and control mice) using the University of Ottawa FACS Core Facility. mRNA was extracted using Arcturus Picopure RNA Isolation Kit (Applied Biosystems, ThermoFisher). .. For nDKO and control samples, RT-PCR was completed using QuantiTect® SYBR® Green RT-PCR Kit in Qiagen Rotor-Gene Q MDx (Qiagen Inc.) and 0.5 ng of mRNA.

    Software:

    Article Title: Clinical performance of an analytically validated assay in comparison to microarray technology to assess PITX2 DNA-methylation in breast cancer
    Article Snippet: After bisulfite exposure to distinguish between methylated and unmethylated PITX2, the percent methylation ration (PMR) of three CpG motifs of the PITX2 gene promoter 2 is quantified by qMSP and calculated by the Rotor-Gene AssayManager® software with Gamma Plug-in plus a kit-specific PITX2 Assay Profile for automated analysis and quality control including all validity criteria. .. PCR analysis is performed on Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, Hilden, Germany).

    Article Title: Epigenetic IVD Tests for Personalized Precision Medicine in Cancer
    Article Snippet: It runs in the real-time Rotor-Gene Q MDx thermal cycler (Qiagen) or a Rotor-Gene Q MDx 5plex HRM instrument (Qiagen). .. The percentage of the methylation ratio (PMR = 100/(1 + 2exp(CtFAM(methylated) − CtHEX(unmethylated))]) is calculated by the Rotor-Gene AssayManager® software with a Gamma Plug-in plus a kit-specific PITX2 Assay Profile for automated analyses and quality control, including all the validity criteria.

    Real-time Polymerase Chain Reaction:

    Article Title: Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat
    Article Snippet: .. Quantitative real time PCR was performed using Rotor Gene Q MDx 5plex HRM (QIAGEN). .. In the qPCR, 18S ribosomal RNA obtained from R. pirica was used as the internal control. qPCR was performed using Rotor-Gene SYBR® Green PCR Kit (QIAGEN) as described in the manufacturer’s protocol.

    Article Title: Surgical and Pathological Changes after Radiofrequency Ablation of Thyroid Nodules
    Article Snippet: .. DNA was extracted from paraffin blocks and tested by using real-time polymerase chain reaction on the Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, UK) and on the PyroMark Q24 System (Qiagen, UK), respectively. ..

    Article Title: Molecular Signature of Indeterminate Thyroid Lesions: Current Methods to Improve Fine Needle Aspiration Cytology (FNAC) Diagnosis
    Article Snippet: Two µL of cDNA was used as template for real time PCR (RT-PCR) to measure miRNA expression levels with the miScript SYBR Green PCR Kit (Qiagen) with specific primers for miRNA-95 and -190 (Qiagen). .. RT-PCR was performed in duplicate on the Rotor-gene Q MDx (Qiagen) under the following cycling conditions: 95 °C for 15 min, 40 cycles at 94 °C for 15 s, 55 °C for 30 s and 70 °C for 30 s. Relative expression levels were calculated using the 2−ΔΔC t method with the miRNA-16 as endogenous control. miRNA expression correctly identified 38/43 (specificity of 88.4%) of the benign lesions and 27/32 (sensitivity of 84.3%) of malignant sample.

    Article Title: Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation
    Article Snippet: Real-Time RT-qPCR Analysis IFN-α, IFN-β, IFN-γ, IL-4, IL-8, IL-15, IL-16, IL-17, and IL-18 mRNA gene expressions were measured using relative-quantitative real-time PCR (RT-qPCR). .. The QuantiFast SYBR® Green PCR Kit (Qiagen, Hilden, Germany) was used in the Rotor-Gene Q MDx 5plex Platform (Qiagen, Hilden, Germany), according to a previously published protocol (Al-Zghoul et al., 2019).

    Article Title: New Drugs/Drug News
    Article Snippet: .. Source: ; The Wall Street Journal , May 1, 2012 Name: Rotor-Gene Q MDx Manufacturer: Qiagen, The Netherlands Approval Date: April 17, 2012 Purpose: This automated molecular detection platform is based on real-time polymerase chain reaction (PCR) technology. .. The system is intended for in vitro diagnostic use with FDA-approved nucleic acid tests, such as those used for hepatitis B and C and HIV infections, in clinical laboratories.

    Article Title: Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice
    Article Snippet: .. SSR-HRM analysis was performed using a Rotor-Gene Q MDx (QIAGEN) real-time PCR machine. .. The HRM reaction mixture contained 50 ng of genomic DNA, 5 μL of 2× HRM master mix (QIAGEN, Germany), 0.5 μL of 10 μmol/L of both forward and reverse primers, and distilled water was added to reach the final volume.

    Article Title: Applying high-resolution melting (HRM) technology to identify five commonly used Artemisia species
    Article Snippet: .. DNA amplification was performed in a Rotor-Gene Q MDx (QIAGEN) real-time PCR under the following conditions: 94 °C for 3 min, followed by 40 cycles of 94 °C for 30 s, 56 °C for 30 s and 72 °C for 45 s. The fluorescent data were acquired at the end of each extension step during the PCR cycles. .. HRM analysis was performed immediately following the completion of PCR amplification.

    Article Title: Epigenetic IVD Tests for Personalized Precision Medicine in Cancer
    Article Snippet: PITX2 methylation is assessed by methylation-specific real-time PCR and exploits the quantitative PCR (qPCR) oligonucleotide hydrolysis principle of two TaqMan probes labeled with different fluorescent dies (FAM™ for fully methylated and HEX™ for fully unmethylated DNA) in combination with methylation nonspecific primers to measure the methylation status of the target sequences of PITX2 gene promoter 2 in bisulfite-treated DNA. .. It runs in the real-time Rotor-Gene Q MDx thermal cycler (Qiagen) or a Rotor-Gene Q MDx 5plex HRM instrument (Qiagen).

    Article Title: Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice
    Article Snippet: .. HRM-PCR Amplification and Data Analysis SSR-HRM analysis was performed using a Rotor-Gene Q MDx (QIAGEN) real-time PCR machine. .. The HRM reaction mixture contained 50 ng of genomic DNA, 5 μL of 2× HRM master mix (QIAGEN, Germany), 0.5 μL of 10 μmol/L of both forward and reverse primers, and distilled water was added to reach the final volume.

    Multiplex Assay:

    Article Title: New Drugs/Drug News
    Article Snippet: Source: ; The Wall Street Journal , May 1, 2012 Name: Rotor-Gene Q MDx Manufacturer: Qiagen, The Netherlands Approval Date: April 17, 2012 Purpose: This automated molecular detection platform is based on real-time polymerase chain reaction (PCR) technology. .. With a choice of up to six excitation sources and six detection filters, combined with a short, fixed optical path, the device can be used for multiplex reactions.

    In Vitro:

    Article Title: Clinical performance of an analytically validated assay in comparison to microarray technology to assess PITX2 DNA-methylation in breast cancer
    Article Snippet: The PITX2 qMSP assay was optimized for the usage as an in vitro diagnostic assay following the CLSI guidelines ( https://clsi.org/ ; see also ). .. PCR analysis is performed on Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, Hilden, Germany).

    Article Title: New Drugs/Drug News
    Article Snippet: Source: ; The Wall Street Journal , May 1, 2012 Name: Rotor-Gene Q MDx Manufacturer: Qiagen, The Netherlands Approval Date: April 17, 2012 Purpose: This automated molecular detection platform is based on real-time polymerase chain reaction (PCR) technology. .. The system is intended for in vitro diagnostic use with FDA-approved nucleic acid tests, such as those used for hepatitis B and C and HIV infections, in clinical laboratories.

    Produced:

    Article Title: Gene expression profiles in Rana pirica tadpoles following exposure to a predation threat
    Article Snippet: The selected cDNA fragments were produced by PCR using gene specific primers and KOD FX Neo (TOYOBO). .. Quantitative real time PCR was performed using Rotor Gene Q MDx 5plex HRM (QIAGEN).

    Marker:

    Article Title: Surgical and Pathological Changes after Radiofrequency Ablation of Thyroid Nodules
    Article Snippet: In one case HBME-1 staining, which is considered a good marker of malignancy [ , ], was also performed and evaluated. .. DNA was extracted from paraffin blocks and tested by using real-time polymerase chain reaction on the Rotor-Gene Q MDx 5plex HRM instrument (Qiagen, UK) and on the PyroMark Q24 System (Qiagen, UK), respectively.

    FACS:

    Article Title: Adult hippocampal neurogenesis occurs in the absence of Presenilin 1 and Presenilin 2
    Article Snippet: Paragraph title: Fluorescence-Activated Cell Sorting (FACS) Analysis and PCR ... For nDKO and control samples, RT-PCR was completed using QuantiTect® SYBR® Green RT-PCR Kit in Qiagen Rotor-Gene Q MDx (Qiagen Inc.) and 0.5 ng of mRNA.

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    Qiagen rotor gene sybr green pcr kit
    Rotor Gene Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rotor gene sybr green pcr kit/product/Qiagen
    Average 90 stars, based on 273 article reviews
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    rotor gene sybr green pcr kit - by Bioz Stars, 2020-01
    90/100 stars
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    90
    Qiagen rotor gene q 2plex hrm system
    Rotor Gene Q 2plex Hrm System, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rotor gene q 2plex hrm system/product/Qiagen
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rotor gene q 2plex hrm system - by Bioz Stars, 2020-01
    90/100 stars
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