rorγt  (Bioss)

Journal: NPJ Biofilms and Microbiomes

Article Title: Lung microbiota-mediated biotransformation of mogroside preserves pulmonary barrier integrity and attenuates PM 2.5 -induced inflammation via NF-κB–Th17 modulations

doi: 10.1038/s41522-026-00992-y

Figure Lengend Snippet: 6-week observation of experimental subjects from different treatment groups. A compares the body weight changes among the control group (Con), PM₂.₅ group (P) and mogroside + PM₂.₅ group (MG + PM 2.5 ), n = 24. B presents the comparison of the average daily feed intake (ADFI) among the different treatment groups. C Illustrates the histological of lung tissues from the control, P, and MP groups, as shown by H&E and Masson staining, including the degree of damage ( D ) and fibrosis ( E ) in specific regions. F Shows the abundance of IL18, TNFα, and MCP1 proteins in lung tissues, ( G ) ( H ): Comparison of Th17/Treg cell-associated transcriptional factors and cytokine’s expression in lung between three different groups. Western blot, protein abundance of NF-κB, FOXP3, RORγt and mRNA level of Tnf-α, Tgf-β, Il-1β, AhR, Foxp3 and Rorγt in lung tissue, n = 8. All data are presented as mean ± standard error of the mean (SEM), and statistical analysis was performed accordingly. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The sections were incubated overnight at 4 °C with primary antibodies against FOXP1 (Mouse-derived FOXP1 antibody, 92% homologous to chicken FOXP3, dilution 1:100, A12685, ABclonal, China) and RORγt (dilution 1:200, A10240, ABclonal, China).

Techniques: Control, Comparison, Staining, Expressing, Western Blot, Quantitative Proteomics

Journal: NPJ Biofilms and Microbiomes

Article Title: Lung microbiota-mediated biotransformation of mogroside preserves pulmonary barrier integrity and attenuates PM 2.5 -induced inflammation via NF-κB–Th17 modulations

doi: 10.1038/s41522-026-00992-y

Figure Lengend Snippet: 8 days observation of experimental subjects from different treatment groups. BALF was collected from 35-day-old broilers from two donor groups: the control group and a group fed a mogroside-supplemented diet for 35 days. Each type of BALF was processed into two variants: unfiltered (BALF with microbiota) and filtered (BALF without microbiota) to distinguish microbial effects, n = 6. A In vivo distribution of mogrosides of dietary MG supplementation study, n = 8. B Illustrates the histological and th17 differentiation protein of lung tissue among the six group by HE, masson and immunohistochemistry (IHC). Scale bars, 100 μm. IOD, integrated optical density. C Fibrosis scores ( D , E ): Semi-quantitative IHC scoring of FOXP1 and RORγt protein expression in lung. F , G Abundance of NF-κB, IL6, TNFα, RORγt and FOXP3 proteins in lung tissues. H The comparison of mRNA expression level of Tgf-β, Foxp3, Rorγt, Il-17a, Il-17f and Il-22 in the lung tissue among groups. n = 6. All data are presented as mean ± standard error of the mean (SEM), and statistical analysis was performed accordingly (One-way ANOVA only vs. PM 2.5 group), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The sections were incubated overnight at 4 °C with primary antibodies against FOXP1 (Mouse-derived FOXP1 antibody, 92% homologous to chicken FOXP3, dilution 1:100, A12685, ABclonal, China) and RORγt (dilution 1:200, A10240, ABclonal, China).

Techniques: Control, In Vivo, Immunohistochemistry, Expressing, Comparison

Journal: NPJ Biofilms and Microbiomes

Article Title: Lung microbiota-mediated biotransformation of mogroside preserves pulmonary barrier integrity and attenuates PM 2.5 -induced inflammation via NF-κB–Th17 modulations

doi: 10.1038/s41522-026-00992-y

Figure Lengend Snippet: A – F Fibrosis scores, the comparison of protein expression level of NF-κb, TNF-α, IL-6, FOXP1, RORγt; ( G – K ) Tgf-β, Foxp3, Rorγt, Il-17a, Il-17f and Il-22 in the lung tissue among groups, two-way ANOVA analysis, normal (control donor BALF without mogroside), MG (donor BALF with mogroside), M: unfilter (BALF with microbiota), N: filter (BALF without microbiota), Control: n = 5, other groups: n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The sections were incubated overnight at 4 °C with primary antibodies against FOXP1 (Mouse-derived FOXP1 antibody, 92% homologous to chicken FOXP3, dilution 1:100, A12685, ABclonal, China) and RORγt (dilution 1:200, A10240, ABclonal, China).

Techniques: Comparison, Expressing, Control

Journal: Cell Death & Disease

Article Title: Th17-driven CD8 + T cells in hUC-MSC and CAR T-cell dual immunotherapy for superior anti-tumor efficacy

doi: 10.1038/s41419-026-08656-7

Figure Lengend Snippet: CD19 CAR T-cells were co-cultured with Raji cells at a E: T ratio of 1: 5, in the presence or not of hUC-MSCs. CAR T-cells were collected for assessment by flow cytometry at 48 h; A Flow cytometry analysis of CD4 + IL17A + CCR6 + cells, demonstrating a significant increase in the Th17 cell subset in the mCAR group, with subsequent downregulation after RORγt inhibition with GSK805. Data are representative of at least three independent experiments ( n = 3); B Increase of CD19 CAR T-cell cytolysis by MSCs was partially reversed by GSK805. ( n = 3); C MSC-upregulated CD94 and NKG2A expression in CD8 + CAR T-cells is reversed by the Th17 differentiation inhibitor GSK805. Bar graph shows the proportional distribution of CD8⁺CD94⁺NKG2A⁺, CD8⁺CD94⁺NKG2A⁻, and CD8⁺CD94⁻ subpopulations within CD8⁺ CAR T-cells across experimental groups. Scatter plots represent schematic distributions of individual data points. D CD19 CAR T-cells were co-cultured with Raji cells at a E: T ratio of 1: 5 in 6-well plates for 48 h. Live cells (GFP⁻FVD⁻) were sorted into three distinct populations based on CD8, CD94, and NKG2A expression: CD8⁺CD94⁺NKG2A⁺, CD8⁺CD94⁺NKG2A⁻, and CD8⁺CD94⁻. RNA was extracted from each population, reverse transcribed into cDNA, and subjected to quantitative PCR (qPCR) analysis to determine the relative mRNA expression levels of TNF, IFN-γ, GZMB, PRF1, NKG7, and GNLY. E Experimental design of the in vivo experiment on the antitumor effect of MSC on CD19 CAR T-cells, which were purified by sorting after lentiviral transfection and expansion in vitro for 7 days before being i.v. injection into mice. The Th17 differentiation inhibitor GSK805 was given by gavage at 10 mg/kg/day ( n = 5); F Tumor growth monitored by BLI at the indicated time points ( n = 4 or 5); G Flow cytometric analysis of human CD8⁺ T cell subsets in BM at day 7 post CAR-T infusion. MSC treatment increased CD8⁺CD94⁺ CAR T-cell frequencies in BM, consistent with in vitro findings. Data represent mean ± SD ( n = 4). One-way ANOVA was used to assess significance in ( A–D , G ). * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Where indicated, the RORγt inhibitor GSK805 (10 μM, MCE, NJ, USA), purified anti-human IL-1β (1 μg/mL, BioLegend), purified anti-human IL-6 (1 μg/mL, BioLegend), recombinant human IL-1β (1 ng/mL, Pepro Tech) and recombinant human IL-6 (10 ng/mL, Pepro Tech) were added to the co-culture system [ ].

Techniques: Cell Culture, Flow Cytometry, Inhibition, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, In Vivo, Purification, Transfection, In Vitro, Injection