rock inhibitor y 27632  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC rock inhibitor y 27632
    Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 - by Bioz Stars, 2024-05
    94/100 stars

    Images

    protein kinase rock inhibitor y 27632  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC protein kinase rock inhibitor y 27632
    ( A ) Representative phase-contrast images show the morphology of cells (Scale bars: 200 μm). Quantitative data on cell number ( B ) and cell viability ( C ) are shown. ( D ) Morphological index, the ratio of longitudinal to the transverse axis. LA: Longitudinal axis; TA: Transverse axis. All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; & p < 0.0001 vs . PP+SB43 group in all time points, 6 and 48 hours.
    Protein Kinase Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein kinase rock inhibitor y 27632/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protein kinase rock inhibitor y 27632 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    1) Product Images from "Hydrostatic pressure mediates epithelial-mesenchymal transition of cholangiocytes through RhoA/ROCK and TGF-β/smad pathways"

    Article Title: Hydrostatic pressure mediates epithelial-mesenchymal transition of cholangiocytes through RhoA/ROCK and TGF-β/smad pathways

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0300548

    ( A ) Representative phase-contrast images show the morphology of cells (Scale bars: 200 μm). Quantitative data on cell number ( B ) and cell viability ( C ) are shown. ( D ) Morphological index, the ratio of longitudinal to the transverse axis. LA: Longitudinal axis; TA: Transverse axis. All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; & p < 0.0001 vs . PP+SB43 group in all time points, 6 and 48 hours.
    Figure Legend Snippet: ( A ) Representative phase-contrast images show the morphology of cells (Scale bars: 200 μm). Quantitative data on cell number ( B ) and cell viability ( C ) are shown. ( D ) Morphological index, the ratio of longitudinal to the transverse axis. LA: Longitudinal axis; TA: Transverse axis. All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; & p < 0.0001 vs . PP+SB43 group in all time points, 6 and 48 hours.

    Techniques Used:

    Immunofluorescent images of vimentin (left panel) and quantitative data (right side) at 6 hours ( A ) and 48 hours ( B ) after treatments (Scale bars in images: 20 μm). Immunofluorescent images of ZEB1 expression (left panel) and quantitative data (right side) at 6 hr ( C ) and 48 hr ( D ) after treatment (Scale bars in images: 50 μm). All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.
    Figure Legend Snippet: Immunofluorescent images of vimentin (left panel) and quantitative data (right side) at 6 hours ( A ) and 48 hours ( B ) after treatments (Scale bars in images: 20 μm). Immunofluorescent images of ZEB1 expression (left panel) and quantitative data (right side) at 6 hr ( C ) and 48 hr ( D ) after treatment (Scale bars in images: 50 μm). All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.

    Techniques Used: Expressing

    Representative images (left) and quantitative data (right bar graph) show the immunostaining of the pSmad2/3 at 48 hr after treatments (Scale bars: 50 μm). All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.
    Figure Legend Snippet: Representative images (left) and quantitative data (right bar graph) show the immunostaining of the pSmad2/3 at 48 hr after treatments (Scale bars: 50 μm). All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.

    Techniques Used: Immunostaining

    Double immunostaining analysis on the expression of collagen 1α (Green) and fibronectin (Red) in cholangiocytes at 48 hr after treatments (Scale bars: 20 μm). All data are presented as mean ± SD from four independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.
    Figure Legend Snippet: Double immunostaining analysis on the expression of collagen 1α (Green) and fibronectin (Red) in cholangiocytes at 48 hr after treatments (Scale bars: 20 μm). All data are presented as mean ± SD from four independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.

    Techniques Used: Double Immunostaining, Expressing

    y 27632 rock inhibitor  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC y 27632 rock inhibitor
    Y 27632 Rock Inhibitor, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/y 27632 rock inhibitor/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    y 27632 rock inhibitor - by Bioz Stars, 2024-05
    86/100 stars

    Images

    rock inhibitor y 27632  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC rock inhibitor y 27632
    Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    rock inhibitor y 27632  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC rock inhibitor y 27632
    Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    rock inhibitor y 27632  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC rock inhibitor y 27632
    Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 - by Bioz Stars, 2024-05
    94/100 stars

    Images

    rock inhibitor y 27632 10µm  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC rock inhibitor y 27632 10µm
    Rock Inhibitor Y 27632 10µm, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632 10µm/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 10µm - by Bioz Stars, 2024-05
    86/100 stars

    Images

    rock inhibitor y 27632 10µm  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC rock inhibitor y 27632 10µm
    Rock Inhibitor Y 27632 10µm, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632 10µm/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 10µm - by Bioz Stars, 2024-05
    86/100 stars

    Images

    rock inhibitor y 27632  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC rock inhibitor y 27632
    Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 - by Bioz Stars, 2024-05
    99/100 stars

    Images

    rock inhibitor y 27632  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC rock inhibitor y 27632
    Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 - by Bioz Stars, 2024-05
    94/100 stars

    Images

    rock inhibitor y 27632  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    ATCC rock inhibitor y 27632
    Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 - by Bioz Stars, 2024-05
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    ATCC rock inhibitor y 27632
    Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632/product/ATCC
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 - by Bioz Stars, 2024-05
    94/100 stars
      Buy from Supplier

    86
    ATCC protein kinase rock inhibitor y 27632
    ( A ) Representative phase-contrast images show the morphology of cells (Scale bars: 200 μm). Quantitative data on cell number ( B ) and cell viability ( C ) are shown. ( D ) Morphological index, the ratio of longitudinal to the transverse axis. LA: Longitudinal axis; TA: Transverse axis. All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; & p < 0.0001 vs . PP+SB43 group in all time points, 6 and 48 hours.
    Protein Kinase Rock Inhibitor Y 27632, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein kinase rock inhibitor y 27632/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protein kinase rock inhibitor y 27632 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    ATCC y 27632 rock inhibitor
    ( A ) Representative phase-contrast images show the morphology of cells (Scale bars: 200 μm). Quantitative data on cell number ( B ) and cell viability ( C ) are shown. ( D ) Morphological index, the ratio of longitudinal to the transverse axis. LA: Longitudinal axis; TA: Transverse axis. All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; & p < 0.0001 vs . PP+SB43 group in all time points, 6 and 48 hours.
    Y 27632 Rock Inhibitor, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/y 27632 rock inhibitor/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    y 27632 rock inhibitor - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    ATCC rock inhibitor y 27632 10µm
    ( A ) Representative phase-contrast images show the morphology of cells (Scale bars: 200 μm). Quantitative data on cell number ( B ) and cell viability ( C ) are shown. ( D ) Morphological index, the ratio of longitudinal to the transverse axis. LA: Longitudinal axis; TA: Transverse axis. All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; & p < 0.0001 vs . PP+SB43 group in all time points, 6 and 48 hours.
    Rock Inhibitor Y 27632 10µm, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rock inhibitor y 27632 10µm/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rock inhibitor y 27632 10µm - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Representative phase-contrast images show the morphology of cells (Scale bars: 200 μm). Quantitative data on cell number ( B ) and cell viability ( C ) are shown. ( D ) Morphological index, the ratio of longitudinal to the transverse axis. LA: Longitudinal axis; TA: Transverse axis. All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; & p < 0.0001 vs . PP+SB43 group in all time points, 6 and 48 hours.

    Journal: PLOS ONE

    Article Title: Hydrostatic pressure mediates epithelial-mesenchymal transition of cholangiocytes through RhoA/ROCK and TGF-β/smad pathways

    doi: 10.1371/journal.pone.0300548

    Figure Lengend Snippet: ( A ) Representative phase-contrast images show the morphology of cells (Scale bars: 200 μm). Quantitative data on cell number ( B ) and cell viability ( C ) are shown. ( D ) Morphological index, the ratio of longitudinal to the transverse axis. LA: Longitudinal axis; TA: Transverse axis. All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; & p < 0.0001 vs . PP+SB43 group in all time points, 6 and 48 hours.

    Article Snippet: When the cells were grown to 50–70% confluence, we randomly kept the cells at following conditions: 1) under normal atmospheric condition in ordinary incubator (AP group); 2) under 50 mmHg hydrostatic pressure induced by a commercial device as described previously [ ] (PP group); 3) under 50 mmHg hydrostatic pressure with the addition of Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM; ATCC, ACS-3030 ™ , USA) in medium (PP+Y27 group); and 4) under 50 mmHg hydrostatic pressure with the addition of SB-431542 (10 μM; MCE, HY-10431, Japan), an inhibitor to activin receptor-like kinase 5 (ALK5, also known as TGF-β type I receptor) (PP+SB43 group).

    Techniques:

    Immunofluorescent images of vimentin (left panel) and quantitative data (right side) at 6 hours ( A ) and 48 hours ( B ) after treatments (Scale bars in images: 20 μm). Immunofluorescent images of ZEB1 expression (left panel) and quantitative data (right side) at 6 hr ( C ) and 48 hr ( D ) after treatment (Scale bars in images: 50 μm). All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.

    Journal: PLOS ONE

    Article Title: Hydrostatic pressure mediates epithelial-mesenchymal transition of cholangiocytes through RhoA/ROCK and TGF-β/smad pathways

    doi: 10.1371/journal.pone.0300548

    Figure Lengend Snippet: Immunofluorescent images of vimentin (left panel) and quantitative data (right side) at 6 hours ( A ) and 48 hours ( B ) after treatments (Scale bars in images: 20 μm). Immunofluorescent images of ZEB1 expression (left panel) and quantitative data (right side) at 6 hr ( C ) and 48 hr ( D ) after treatment (Scale bars in images: 50 μm). All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.

    Article Snippet: When the cells were grown to 50–70% confluence, we randomly kept the cells at following conditions: 1) under normal atmospheric condition in ordinary incubator (AP group); 2) under 50 mmHg hydrostatic pressure induced by a commercial device as described previously [ ] (PP group); 3) under 50 mmHg hydrostatic pressure with the addition of Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM; ATCC, ACS-3030 ™ , USA) in medium (PP+Y27 group); and 4) under 50 mmHg hydrostatic pressure with the addition of SB-431542 (10 μM; MCE, HY-10431, Japan), an inhibitor to activin receptor-like kinase 5 (ALK5, also known as TGF-β type I receptor) (PP+SB43 group).

    Techniques: Expressing

    Representative images (left) and quantitative data (right bar graph) show the immunostaining of the pSmad2/3 at 48 hr after treatments (Scale bars: 50 μm). All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.

    Journal: PLOS ONE

    Article Title: Hydrostatic pressure mediates epithelial-mesenchymal transition of cholangiocytes through RhoA/ROCK and TGF-β/smad pathways

    doi: 10.1371/journal.pone.0300548

    Figure Lengend Snippet: Representative images (left) and quantitative data (right bar graph) show the immunostaining of the pSmad2/3 at 48 hr after treatments (Scale bars: 50 μm). All data are presented as mean ± SD from three independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.

    Article Snippet: When the cells were grown to 50–70% confluence, we randomly kept the cells at following conditions: 1) under normal atmospheric condition in ordinary incubator (AP group); 2) under 50 mmHg hydrostatic pressure induced by a commercial device as described previously [ ] (PP group); 3) under 50 mmHg hydrostatic pressure with the addition of Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM; ATCC, ACS-3030 ™ , USA) in medium (PP+Y27 group); and 4) under 50 mmHg hydrostatic pressure with the addition of SB-431542 (10 μM; MCE, HY-10431, Japan), an inhibitor to activin receptor-like kinase 5 (ALK5, also known as TGF-β type I receptor) (PP+SB43 group).

    Techniques: Immunostaining

    Double immunostaining analysis on the expression of collagen 1α (Green) and fibronectin (Red) in cholangiocytes at 48 hr after treatments (Scale bars: 20 μm). All data are presented as mean ± SD from four independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.

    Journal: PLOS ONE

    Article Title: Hydrostatic pressure mediates epithelial-mesenchymal transition of cholangiocytes through RhoA/ROCK and TGF-β/smad pathways

    doi: 10.1371/journal.pone.0300548

    Figure Lengend Snippet: Double immunostaining analysis on the expression of collagen 1α (Green) and fibronectin (Red) in cholangiocytes at 48 hr after treatments (Scale bars: 20 μm). All data are presented as mean ± SD from four independent experiments. AP: atmospheric pressure; PP: positive pressure; PP+Y27: positive pressure plus ROCK inhibitor Y-27632. PP+SB43: positive pressure plus ALK5 inhibitor SB-431542. * p < 0.0001 vs . AP group; # p < 0.0001 vs . PP group; $ p < 0.0001 vs . PP+SB43 group.

    Article Snippet: When the cells were grown to 50–70% confluence, we randomly kept the cells at following conditions: 1) under normal atmospheric condition in ordinary incubator (AP group); 2) under 50 mmHg hydrostatic pressure induced by a commercial device as described previously [ ] (PP group); 3) under 50 mmHg hydrostatic pressure with the addition of Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM; ATCC, ACS-3030 ™ , USA) in medium (PP+Y27 group); and 4) under 50 mmHg hydrostatic pressure with the addition of SB-431542 (10 μM; MCE, HY-10431, Japan), an inhibitor to activin receptor-like kinase 5 (ALK5, also known as TGF-β type I receptor) (PP+SB43 group).

    Techniques: Double Immunostaining, Expressing