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Roche roche qrt pcr assay
Expression of SMN2 mRNA in SMA patients and healthy controls. SMN2 mRNA was isolated from blood and analyzed using <t>qRT-PCR.</t> Expression level is calculated using 2ˆ-deltaCp of the reference gene. There is a strong overlap of SMN2 mRNA in the different patient groups. Note that in the healthy controls SMN2 levels are lower than levels in patients with the same SMN2 copy number.
Roche Qrt Pcr Assay, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Biomarker for Spinal Muscular Atrophy: Expression of SMN in Peripheral Blood of SMA Patients and Healthy Controls"

Article Title: Biomarker for Spinal Muscular Atrophy: Expression of SMN in Peripheral Blood of SMA Patients and Healthy Controls

Journal: PLoS ONE

doi: 10.1371/journal.pone.0139950

Expression of SMN2 mRNA in SMA patients and healthy controls. SMN2 mRNA was isolated from blood and analyzed using qRT-PCR. Expression level is calculated using 2ˆ-deltaCp of the reference gene. There is a strong overlap of SMN2 mRNA in the different patient groups. Note that in the healthy controls SMN2 levels are lower than levels in patients with the same SMN2 copy number.
Figure Legend Snippet: Expression of SMN2 mRNA in SMA patients and healthy controls. SMN2 mRNA was isolated from blood and analyzed using qRT-PCR. Expression level is calculated using 2ˆ-deltaCp of the reference gene. There is a strong overlap of SMN2 mRNA in the different patient groups. Note that in the healthy controls SMN2 levels are lower than levels in patients with the same SMN2 copy number.

Techniques Used: Expressing, Isolation, Quantitative RT-PCR

Related Articles

DNA Extraction:

Article Title: Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients ▿
Article Snippet: Nevertheless, our data suggested that the sensitivity of the Nanogen PCR assay may have been underestimated. (ii) The QRT-PCR assay from Nanogen yielded significantly higher CMV DNA loads than the other two PCR assays, regardless of the platform used for DNA extraction and the CMV DNA contents of the specimens. .. Interestingly, we found that the CMV DNA loads obtained using the Abbott QRT-PCR assay coupled with the m24 SP extraction system were slightly higher than those obtained by the Roche QRT-PCR assay coupled with the AmpliPrep extraction method for specimens with high CMV DNA concentrations ( > 10,000 copies/ml), but they were slightly lower for specimens with intermediate CMV DNA contents ( > 1,000 but < 10,000 copies/ml).

Flow Cytometry:

Article Title: Biomarker for Spinal Muscular Atrophy: Expression of SMN in Peripheral Blood of SMA Patients and Healthy Controls
Article Snippet: Paragraph title: Supporting Information Transparent reporting of clinical trials CONSORT 2010 Flow Diagram. Comparison of newly developed SMN mRNA and SMN protein assays with previously published assays. Study protocol. Raw data of the assay. TREND Checklist. ... Comparison of Roche qRT-PCR assay on COBAS (SMN2 mRNA assay B) with SMN2 mRNA assay B ([ ]et al).

Intra Assay:

Article Title: Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients ▿
Article Snippet: These differences were overall of greater magnitude than those that would be expected according to the intra-assay variability of the respective QRT-PCR assays, and they were greatest at the lowest CMV DNA concentration. .. Interestingly, we found that the CMV DNA loads obtained using the Abbott QRT-PCR assay coupled with the m24 SP extraction system were slightly higher than those obtained by the Roche QRT-PCR assay coupled with the AmpliPrep extraction method for specimens with high CMV DNA concentrations ( > 10,000 copies/ml), but they were slightly lower for specimens with intermediate CMV DNA contents ( > 1,000 but < 10,000 copies/ml).

Infection:

Article Title: Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients ▿
Article Snippet: Interestingly, we found that the CMV DNA loads obtained using the Abbott QRT-PCR assay coupled with the m24 SP extraction system were slightly higher than those obtained by the Roche QRT-PCR assay coupled with the AmpliPrep extraction method for specimens with high CMV DNA concentrations ( > 10,000 copies/ml), but they were slightly lower for specimens with intermediate CMV DNA contents ( > 1,000 but < 10,000 copies/ml). .. The above findings have relevant implications for the therapeutic management of active CMV infection in Allo-SCT recipients.

Concentration Assay:

Article Title: Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients ▿
Article Snippet: These differences were overall of greater magnitude than those that would be expected according to the intra-assay variability of the respective QRT-PCR assays, and they were greatest at the lowest CMV DNA concentration. .. Interestingly, we found that the CMV DNA loads obtained using the Abbott QRT-PCR assay coupled with the m24 SP extraction system were slightly higher than those obtained by the Roche QRT-PCR assay coupled with the AmpliPrep extraction method for specimens with high CMV DNA concentrations ( > 10,000 copies/ml), but they were slightly lower for specimens with intermediate CMV DNA contents ( > 1,000 but < 10,000 copies/ml).

Quantitative RT-PCR:

Article Title: Biomarker for Spinal Muscular Atrophy: Expression of SMN in Peripheral Blood of SMA Patients and Healthy Controls
Article Snippet: .. Comparison of Roche qRT-PCR assay on COBAS (SMN2 mRNA assay B) with SMN2 mRNA assay B ([ ]et al). .. Note the group of values at the left side of the graph which are moved due to low expression of the GAPDH reference gene as used by Naryshkin et al and a resulting decrease in the delta Cp values.

Article Title: Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients ▿
Article Snippet: .. Interestingly, we found that the CMV DNA loads obtained using the Abbott QRT-PCR assay coupled with the m24 SP extraction system were slightly higher than those obtained by the Roche QRT-PCR assay coupled with the AmpliPrep extraction method for specimens with high CMV DNA concentrations ( > 10,000 copies/ml), but they were slightly lower for specimens with intermediate CMV DNA contents ( > 1,000 but < 10,000 copies/ml). .. In this context, Hanson et al. ( ) compared the Roche CMV UL54 analyte-specific reagent (equivalent to the Roche QRT-PCR assay used in the present study) and the Qiagen realArt CMV LightCycler PCR reagent (essentially the same product as the Abbott QRT-PCR assay used in the current study) and found that the CMV plasma DNA loads measured using the Qiagen assay tended to be lower than those measured with the Roche reagent when using clinical samples but not when employing the OptiQuant panel, in both cases after DNA extraction with the Roche MagnaPure automated platform. (iii) The performance characteristics of the QRT-PCR assays evaluated herein for clinical samples were extensible at cell-based standards from AcroMetrix, despite relevant differences in viral DNA conformation (highly fragmented CMV DNA in plasma specimens and large amounts of concatemers in cell-derived preparations) ( , ).

Expressing:

Article Title: Biomarker for Spinal Muscular Atrophy: Expression of SMN in Peripheral Blood of SMA Patients and Healthy Controls
Article Snippet: Comparison of Roche qRT-PCR assay on COBAS (SMN2 mRNA assay B) with SMN2 mRNA assay B ([ ]et al). .. Note the group of values at the left side of the graph which are moved due to low expression of the GAPDH reference gene as used by Naryshkin et al and a resulting decrease in the delta Cp values.

Polymerase Chain Reaction:

Article Title: Comparative Evaluation of Three Automated Systems for DNA Extraction in Conjunction with Three Commercially Available Real-Time PCR Assays for Quantitation of Plasma Cytomegalovirus DNAemia in Allogeneic Stem Cell Transplant Recipients ▿
Article Snippet: The CMV DNA loads measured by the Abbott PCR and the Roche PCR assays were not significantly different, irrespective of the extraction method and the CMV DNA content of specimens. .. Interestingly, we found that the CMV DNA loads obtained using the Abbott QRT-PCR assay coupled with the m24 SP extraction system were slightly higher than those obtained by the Roche QRT-PCR assay coupled with the AmpliPrep extraction method for specimens with high CMV DNA concentrations ( > 10,000 copies/ml), but they were slightly lower for specimens with intermediate CMV DNA contents ( > 1,000 but < 10,000 copies/ml).

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  • 86
    Roche qrt pcr roche lightcycler technology
    ASPP2 mRNA expression in acute leukemia. <t>qRT-PCR</t> based mRNA expression levels are displayed after normalizing to a healthy blood donor (set as 1) on a logarithmic scale. Cohort analysis reveals significant lower ASPP2 levels for an acute leukemia population compared to a healthy peripheral blood and bone marrow donor cohort (A). Comparison of prognostic risk groups confirms lower ASPP2 expression levels for the good-risk as well as higher-risk cohort when compared to a healthy donor population – whereas attenuated ASPP2 expression levels are more pronounced and statistically significantly different for the higher-risk cohort (B). Analysis of therapy responders (i.e. achievement of complete remission after one cycle of induction chemotherapy) demonstrates significantly lower ASPP2 levels for the therapy-failure population when compared to the responder cohort (including good-/higher-risk pts.) (C). ROC curve analysis defining the ideal threshold to distinguish a definite non-responding sub-population is shown in figure 1D (i.e. patients with attenuated ASPP2 expression levels ≤0.8 are likely not to respond to induction chemotherapy (with no single falsely positive tested patient at this threshold). P-values are provided as indicated by an asterix. Patient characteristics, including definitions of the prognostic risk groups, are summarized in Table 1 and 2.
    Qrt Pcr Roche Lightcycler Technology, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr roche lightcycler technology/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qrt pcr roche lightcycler technology - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    88
    Roche roche qrt pcr assay
    Expression of SMN2 mRNA in SMA patients and healthy controls. SMN2 mRNA was isolated from blood and analyzed using <t>qRT-PCR.</t> Expression level is calculated using 2ˆ-deltaCp of the reference gene. There is a strong overlap of SMN2 mRNA in the different patient groups. Note that in the healthy controls SMN2 levels are lower than levels in patients with the same SMN2 copy number.
    Roche Qrt Pcr Assay, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/roche qrt pcr assay/product/Roche
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    roche qrt pcr assay - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    92
    Roche qrt pcr roche lightcycler 480 system
    Immune responses of CiIFN1/3 to plasmids containing CpG ODNs Left, the mRNA expressions of CiIFN1 (A) and CiIFN3 (B) were measured at 24 and 48 h post-infection. CIK cells were stimulated with PBS (control) or plasmids containing CpG ODNs and infected with GCRV. Other captions were the same as Figure 3 . Right, CIK cells were co-transfected with 800 ng of pRL-TK and IFN1pro-luc (A) or IFN3pro-luc (B) in 24-well plates. At 16 h post-transfection, the cells were stimulated with PBS (control) or plasmids and infected with GCRV for 16 h or uninfected. Dual luciferase reporter assays were conducted at 12 h after GCRV infection. Treatment durations in this assay was far shorter than those in <t>qRT-PCR</t> which caused different results. Error bars indicate standard deviation (n = 4). Asterisks indicate significant difference from control (*, P ≤ 0.05).
    Qrt Pcr Roche Lightcycler 480 System, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr roche lightcycler 480 system/product/Roche
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qrt pcr roche lightcycler 480 system - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    ASPP2 mRNA expression in acute leukemia. qRT-PCR based mRNA expression levels are displayed after normalizing to a healthy blood donor (set as 1) on a logarithmic scale. Cohort analysis reveals significant lower ASPP2 levels for an acute leukemia population compared to a healthy peripheral blood and bone marrow donor cohort (A). Comparison of prognostic risk groups confirms lower ASPP2 expression levels for the good-risk as well as higher-risk cohort when compared to a healthy donor population – whereas attenuated ASPP2 expression levels are more pronounced and statistically significantly different for the higher-risk cohort (B). Analysis of therapy responders (i.e. achievement of complete remission after one cycle of induction chemotherapy) demonstrates significantly lower ASPP2 levels for the therapy-failure population when compared to the responder cohort (including good-/higher-risk pts.) (C). ROC curve analysis defining the ideal threshold to distinguish a definite non-responding sub-population is shown in figure 1D (i.e. patients with attenuated ASPP2 expression levels ≤0.8 are likely not to respond to induction chemotherapy (with no single falsely positive tested patient at this threshold). P-values are provided as indicated by an asterix. Patient characteristics, including definitions of the prognostic risk groups, are summarized in Table 1 and 2.

    Journal: PLoS ONE

    Article Title: Attenuated Expression of Apoptosis Stimulating Protein of p53-2 (ASPP2) in Human Acute Leukemia Is Associated with Therapy Failure

    doi: 10.1371/journal.pone.0080193

    Figure Lengend Snippet: ASPP2 mRNA expression in acute leukemia. qRT-PCR based mRNA expression levels are displayed after normalizing to a healthy blood donor (set as 1) on a logarithmic scale. Cohort analysis reveals significant lower ASPP2 levels for an acute leukemia population compared to a healthy peripheral blood and bone marrow donor cohort (A). Comparison of prognostic risk groups confirms lower ASPP2 expression levels for the good-risk as well as higher-risk cohort when compared to a healthy donor population – whereas attenuated ASPP2 expression levels are more pronounced and statistically significantly different for the higher-risk cohort (B). Analysis of therapy responders (i.e. achievement of complete remission after one cycle of induction chemotherapy) demonstrates significantly lower ASPP2 levels for the therapy-failure population when compared to the responder cohort (including good-/higher-risk pts.) (C). ROC curve analysis defining the ideal threshold to distinguish a definite non-responding sub-population is shown in figure 1D (i.e. patients with attenuated ASPP2 expression levels ≤0.8 are likely not to respond to induction chemotherapy (with no single falsely positive tested patient at this threshold). P-values are provided as indicated by an asterix. Patient characteristics, including definitions of the prognostic risk groups, are summarized in Table 1 and 2.

    Article Snippet: ASPP2 mRNA expression levels, relative to GAPD as the housekeeping gene, were determined by qRT-PCR Roche® LightCycler Technology (Roche, Basel, Switzerland).

    Techniques: Expressing, Quantitative RT-PCR

    Expression of SMN2 mRNA in SMA patients and healthy controls. SMN2 mRNA was isolated from blood and analyzed using qRT-PCR. Expression level is calculated using 2ˆ-deltaCp of the reference gene. There is a strong overlap of SMN2 mRNA in the different patient groups. Note that in the healthy controls SMN2 levels are lower than levels in patients with the same SMN2 copy number.

    Journal: PLoS ONE

    Article Title: Biomarker for Spinal Muscular Atrophy: Expression of SMN in Peripheral Blood of SMA Patients and Healthy Controls

    doi: 10.1371/journal.pone.0139950

    Figure Lengend Snippet: Expression of SMN2 mRNA in SMA patients and healthy controls. SMN2 mRNA was isolated from blood and analyzed using qRT-PCR. Expression level is calculated using 2ˆ-deltaCp of the reference gene. There is a strong overlap of SMN2 mRNA in the different patient groups. Note that in the healthy controls SMN2 levels are lower than levels in patients with the same SMN2 copy number.

    Article Snippet: Comparison of Roche qRT-PCR assay on COBAS (SMN2 mRNA assay B) with SMN2 mRNA assay B ([ ]et al).

    Techniques: Expressing, Isolation, Quantitative RT-PCR

    Immune responses of CiIFN1/3 to plasmids containing CpG ODNs Left, the mRNA expressions of CiIFN1 (A) and CiIFN3 (B) were measured at 24 and 48 h post-infection. CIK cells were stimulated with PBS (control) or plasmids containing CpG ODNs and infected with GCRV. Other captions were the same as Figure 3 . Right, CIK cells were co-transfected with 800 ng of pRL-TK and IFN1pro-luc (A) or IFN3pro-luc (B) in 24-well plates. At 16 h post-transfection, the cells were stimulated with PBS (control) or plasmids and infected with GCRV for 16 h or uninfected. Dual luciferase reporter assays were conducted at 12 h after GCRV infection. Treatment durations in this assay was far shorter than those in qRT-PCR which caused different results. Error bars indicate standard deviation (n = 4). Asterisks indicate significant difference from control (*, P ≤ 0.05).

    Journal: Oncotarget

    Article Title: A plasmid containing CpG ODN as vaccine adjuvant against grass carp reovirus in grass carp Ctenopharyngodon idella

    doi: 10.18632/oncotarget.21245

    Figure Lengend Snippet: Immune responses of CiIFN1/3 to plasmids containing CpG ODNs Left, the mRNA expressions of CiIFN1 (A) and CiIFN3 (B) were measured at 24 and 48 h post-infection. CIK cells were stimulated with PBS (control) or plasmids containing CpG ODNs and infected with GCRV. Other captions were the same as Figure 3 . Right, CIK cells were co-transfected with 800 ng of pRL-TK and IFN1pro-luc (A) or IFN3pro-luc (B) in 24-well plates. At 16 h post-transfection, the cells were stimulated with PBS (control) or plasmids and infected with GCRV for 16 h or uninfected. Dual luciferase reporter assays were conducted at 12 h after GCRV infection. Treatment durations in this assay was far shorter than those in qRT-PCR which caused different results. Error bars indicate standard deviation (n = 4). Asterisks indicate significant difference from control (*, P ≤ 0.05).

    Article Snippet: qRT-PCR Roche LightCycler® 480 system was used to quantify the mRNA expression of the listed genes followed by their GenBank accession number: CiTLR9 (FJ969850), CiRIG-I (GQ478334), CiIFN1 (DQ357216), CiIFN3 (KU182642), CiIFNγ2 (AGQ16237), CiIL-2 (AF486820), CiIL-12 (KF944668), NFκB1 (nuclear factor-kappaB 1) (KY613788), NFκB2 (KY613789), VP4 (GQ469997), CiIgM (DQ417927), CiIgD (GQ429174), CiIgZ (GQ201421), CiTNF-α (HQ696609), CiMx2 (JF699168) and endogenous reference (EF1α, GQ266394 and 18S rRNA, EU047719) using BioEasy Master Mix (SYBR Green) (Hangzhou Bioer Technology Co., Ltd, China).

    Techniques: Infection, Transfection, Luciferase, Quantitative RT-PCR, Standard Deviation