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SLIT2 LTD robo1 binding hs structures
SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized <t>Robo1</t> on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1
Robo1 Binding Hs Structures, supplied by SLIT2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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robo1 binding hs structures - by Bioz Stars, 2020-09
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1) Product Images from "Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1"

Article Title: Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1

Journal: Journal of the American Chemical Society

doi: 10.1021/jacs.6b08161

SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized Robo1 on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1
Figure Legend Snippet: SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized Robo1 on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1

Techniques Used: SPR Assay, Concentration Assay, Binding Assay, Chromatin Immunoprecipitation

Robo1-binding octa- and tetrasaccharides reverted the inhibition of Slit2–Robo1 signaling on mouse diaphragm endothelial cell migration. Serum-starved immortalized mouse lung endothelial cells in serum-free medium were seeded into the upper chamber
Figure Legend Snippet: Robo1-binding octa- and tetrasaccharides reverted the inhibition of Slit2–Robo1 signaling on mouse diaphragm endothelial cell migration. Serum-starved immortalized mouse lung endothelial cells in serum-free medium were seeded into the upper chamber

Techniques Used: Binding Assay, Inhibition, Migration

Composition analysis of the octasaccharide mixture, the GFP-bound fraction, and the Robo1-bound fraction. Each composition is given as follows: [ΔHexA, HexA, GlcN, Ac, SO 3 ]. Abundances are relative to the overall amount of HS detected in that
Figure Legend Snippet: Composition analysis of the octasaccharide mixture, the GFP-bound fraction, and the Robo1-bound fraction. Each composition is given as follows: [ΔHexA, HexA, GlcN, Ac, SO 3 ]. Abundances are relative to the overall amount of HS detected in that

Techniques Used:

Related Articles

Binding Assay:

Article Title: Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1
Article Snippet: .. To determine if the deduced Robo1 binding HS structures modulate Slit2–Robo1 signaling in a cellular context, the Robo1-binding octasaccharide ( 1 ), the Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA-GlcNS6S ( 19 ), and the non-Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA2S-GlcNS6S ( 20 ) were tested by cosupplementing with Slit2 in the lower chamber in the assay ( ). .. The addition of the Robo1-binding oligosaccharides 1 and 19 , but not the non-Robo1-binding oligosaccharide 20 , increased the number of endothelial cells that migrated into the lower chamber side, showing that the added Robo1-binding oligosaccharides inhibited Slit2–Robo1 signaling in the assay.

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    SLIT2 LTD robo1 binding hs structures
    SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized <t>Robo1</t> on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1
    Robo1 Binding Hs Structures, supplied by SLIT2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/robo1 binding hs structures/product/SLIT2 LTD
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    robo1 binding hs structures - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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    SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized Robo1 on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1

    Journal: Journal of the American Chemical Society

    Article Title: Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1

    doi: 10.1021/jacs.6b08161

    Figure Lengend Snippet: SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized Robo1 on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1

    Article Snippet: To determine if the deduced Robo1 binding HS structures modulate Slit2–Robo1 signaling in a cellular context, the Robo1-binding octasaccharide ( 1 ), the Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA-GlcNS6S ( 19 ), and the non-Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA2S-GlcNS6S ( 20 ) were tested by cosupplementing with Slit2 in the lower chamber in the assay ( ).

    Techniques: SPR Assay, Concentration Assay, Binding Assay, Chromatin Immunoprecipitation

    Robo1-binding octa- and tetrasaccharides reverted the inhibition of Slit2–Robo1 signaling on mouse diaphragm endothelial cell migration. Serum-starved immortalized mouse lung endothelial cells in serum-free medium were seeded into the upper chamber

    Journal: Journal of the American Chemical Society

    Article Title: Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1

    doi: 10.1021/jacs.6b08161

    Figure Lengend Snippet: Robo1-binding octa- and tetrasaccharides reverted the inhibition of Slit2–Robo1 signaling on mouse diaphragm endothelial cell migration. Serum-starved immortalized mouse lung endothelial cells in serum-free medium were seeded into the upper chamber

    Article Snippet: To determine if the deduced Robo1 binding HS structures modulate Slit2–Robo1 signaling in a cellular context, the Robo1-binding octasaccharide ( 1 ), the Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA-GlcNS6S ( 19 ), and the non-Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA2S-GlcNS6S ( 20 ) were tested by cosupplementing with Slit2 in the lower chamber in the assay ( ).

    Techniques: Binding Assay, Inhibition, Migration

    Composition analysis of the octasaccharide mixture, the GFP-bound fraction, and the Robo1-bound fraction. Each composition is given as follows: [ΔHexA, HexA, GlcN, Ac, SO 3 ]. Abundances are relative to the overall amount of HS detected in that

    Journal: Journal of the American Chemical Society

    Article Title: Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1

    doi: 10.1021/jacs.6b08161

    Figure Lengend Snippet: Composition analysis of the octasaccharide mixture, the GFP-bound fraction, and the Robo1-bound fraction. Each composition is given as follows: [ΔHexA, HexA, GlcN, Ac, SO 3 ]. Abundances are relative to the overall amount of HS detected in that

    Article Snippet: To determine if the deduced Robo1 binding HS structures modulate Slit2–Robo1 signaling in a cellular context, the Robo1-binding octasaccharide ( 1 ), the Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA-GlcNS6S ( 19 ), and the non-Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA2S-GlcNS6S ( 20 ) were tested by cosupplementing with Slit2 in the lower chamber in the assay ( ).

    Techniques: