rneazy micro kit  (Qiagen)

 
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    Name:
    RNeasy Micro Kit
    Description:
    For purification of up to 45 µg total RNA from cell and tissue samples Kit contents Qiagen RNeasy Micro Kit 50 preps 10 to 14L Elution Volume 5mg Sample Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes 50 RNeasy MinElute Spin Columns Collection Tubes 1 5mL and 2mL RNase free DNase I Carrier RNA RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from very small amounts of starting material No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation
    Catalog Number:
    74004
    Price:
    492
    Category:
    RNeasy Micro Kit
    Buy from Supplier


    Structured Review

    Qiagen rneazy micro kit
    RNeasy Micro Kit
    For purification of up to 45 µg total RNA from cell and tissue samples Kit contents Qiagen RNeasy Micro Kit 50 preps 10 to 14L Elution Volume 5mg Sample Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes 50 RNeasy MinElute Spin Columns Collection Tubes 1 5mL and 2mL RNase free DNase I Carrier RNA RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from very small amounts of starting material No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation
    https://www.bioz.com/result/rneazy micro kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rneazy micro kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. Ten days post-differentiation, EBs in the supernatant were harvested by centrifugation (BeckmanAllegra-6R, 1000 rpm, 5 min) and RNA was isolated using the RNeasy Micro Kit (Qiagen). .. Total RNA (500 ng) was reverse-transcribed using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 and used as template in subsequent PCR with Taq DNA Polymerase.

    Isolation:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. Ten days post-differentiation, EBs in the supernatant were harvested by centrifugation (BeckmanAllegra-6R, 1000 rpm, 5 min) and RNA was isolated using the RNeasy Micro Kit (Qiagen). .. Total RNA (500 ng) was reverse-transcribed using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 and used as template in subsequent PCR with Taq DNA Polymerase.

    Article Title: Global Array-Based Transcriptomics from Minimal Input RNA Utilising an Optimal RNA Isolation Process Combined with SPIA cDNA Probes
    Article Snippet: .. Total RNA from minimal cell numbers was also extracted using the RNeasy Micro RNA isolation kit with the following modifications (process B) ( ). ..

    Article Title: Non‐invasive lung cancer diagnosis by detection of GATA6 and NKX2‐1 isoforms in exhaled breath condensate
    Article Snippet: .. Total RNA was isolated using the RNeasy Micro Kit (Qiagen), and isoform‐specific expression analysis was performed as explained above. .. Total protein extracts from control and LC snap‐frozen tissue samples were analyzed by Western blotting following standard protocols (Singh et al , ) and using antibodies specific for CD63 (ab8219, Abcam), TSG101 (sc‐7964, Santa Cruz), and ACTB (ab6276, Abcam).

    Article Title: Gene Expression Analysis of In Vivo Fluorescent Cells
    Article Snippet: .. Conventional methods RNA was isolated either by the TRIzol (Invitrogen, Germany) method or by use of an RNeasy Micro Kit (QIAGEN, Germany) according to manufacturers' recommendations. .. RNA amplification Total RNA (2–3 ng) was amplified using the MessageAmp II aRNA Amplification Kit (Ambion, USA) according to manufacturer's recommendations.

    Article Title: Non‐invasive lung cancer diagnosis by detection of GATA6 and NKX2‐1 isoforms in exhaled breath condensate
    Article Snippet: .. Total RNA isolation from EBC was performed using 500 μl of sample and the RNeasy Micro Kit (Qiagen). .. Complementary DNA (cDNA) was synthetized using the High Capacity cDNA Reverse Transcription kit (Applied Biosystem) with 0.5–0.7 μg (EBC) or 1 μg (FFPE sample) total RNA.

    RNA Extraction:

    Article Title: Primary versus castration-resistant prostate cancer: modeling through novel murine prostate cancer cell lines
    Article Snippet: .. RNA extraction and Quantitative real time PCR (qRT-PCR) RNeasy Micro Kit (Qiagen) was used to extract total RNA from 1 × 106 PLum-AD and PLum-AI cells. .. Super Script III First Strand Synthesis System for RT-PCR (Invitrogen) was used to generate cDNA from the extracted total RNA.

    Quantitative RT-PCR:

    Article Title: Primary versus castration-resistant prostate cancer: modeling through novel murine prostate cancer cell lines
    Article Snippet: .. RNA extraction and Quantitative real time PCR (qRT-PCR) RNeasy Micro Kit (Qiagen) was used to extract total RNA from 1 × 106 PLum-AD and PLum-AI cells. .. Super Script III First Strand Synthesis System for RT-PCR (Invitrogen) was used to generate cDNA from the extracted total RNA.

    Purification:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: .. One volume of freshly prepared RNase-free 70% ethanol was added to the lysate and total RNA purification was performed by following RNeasy® Micro user guide (QIAGEN, Cat #74004). .. RNase-free DNase set (QIAGEN, Cat#79254) was utilized to remove genomic DNA that can interfere with downstream applications.

    Real-time Polymerase Chain Reaction:

    Article Title: Primary versus castration-resistant prostate cancer: modeling through novel murine prostate cancer cell lines
    Article Snippet: .. RNA extraction and Quantitative real time PCR (qRT-PCR) RNeasy Micro Kit (Qiagen) was used to extract total RNA from 1 × 106 PLum-AD and PLum-AI cells. .. Super Script III First Strand Synthesis System for RT-PCR (Invitrogen) was used to generate cDNA from the extracted total RNA.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. RT-PCR and PCR Total RNA was harvested using RNeasy Micro Kit (Qiagen) and quantified by spectrophotometer. .. 500 ng of RNA was used for cDNA synthesis using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 (Invitrogen).

    Spectrophotometry:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. RT-PCR and PCR Total RNA was harvested using RNeasy Micro Kit (Qiagen) and quantified by spectrophotometer. .. 500 ng of RNA was used for cDNA synthesis using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 (Invitrogen).

    Expressing:

    Article Title: Non‐invasive lung cancer diagnosis by detection of GATA6 and NKX2‐1 isoforms in exhaled breath condensate
    Article Snippet: .. Total RNA was isolated using the RNeasy Micro Kit (Qiagen), and isoform‐specific expression analysis was performed as explained above. .. Total protein extracts from control and LC snap‐frozen tissue samples were analyzed by Western blotting following standard protocols (Singh et al , ) and using antibodies specific for CD63 (ab8219, Abcam), TSG101 (sc‐7964, Santa Cruz), and ACTB (ab6276, Abcam).

    Polymerase Chain Reaction:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. RT-PCR and PCR Total RNA was harvested using RNeasy Micro Kit (Qiagen) and quantified by spectrophotometer. .. 500 ng of RNA was used for cDNA synthesis using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 (Invitrogen).

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  • 99
    Qiagen rneazy micro kit
    Rneazy Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneazy micro kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rneazy micro kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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