rneasy total rna minikit  (Qiagen)


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    Name:
    RNeasy Plus Mini Kit
    Description:
    For phenol free total RNA extraction from cells tissues with removal of genomic DNA contamination Kit contents Qiagen RNeasy Plus Mini Kit 50 preps 30mg Sample 30 to 50L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format 25 min Time Run Ideal for PCR Northern Dot and Slot Blotting Array Analysis Sequencing For Purification of Up to 100g Total RNA from Cells tissues Using gDNA Eliminator Columns Includes RNeasy Mini Spin Columns gDNA Eliminator Spin Columns Collection Tubes RNase free Water and Buffers Benefits Efficient gDNA removal with unique gDNA Eliminator columns no need for DNase High quality total RNA in minutes using fast and simple extraction protocols Phenol free RNA isolation Ideal for sensitive applications such as real time RT PCR and RNA Seq
    Catalog Number:
    74134
    Price:
    360
    Category:
    RNeasy Plus Micro and Mini Kits
    Buy from Supplier


    Structured Review

    Qiagen rneasy total rna minikit
    RNeasy Plus Mini Kit
    For phenol free total RNA extraction from cells tissues with removal of genomic DNA contamination Kit contents Qiagen RNeasy Plus Mini Kit 50 preps 30mg Sample 30 to 50L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format 25 min Time Run Ideal for PCR Northern Dot and Slot Blotting Array Analysis Sequencing For Purification of Up to 100g Total RNA from Cells tissues Using gDNA Eliminator Columns Includes RNeasy Mini Spin Columns gDNA Eliminator Spin Columns Collection Tubes RNase free Water and Buffers Benefits Efficient gDNA removal with unique gDNA Eliminator columns no need for DNase High quality total RNA in minutes using fast and simple extraction protocols Phenol free RNA isolation Ideal for sensitive applications such as real time RT PCR and RNA Seq
    https://www.bioz.com/result/rneasy total rna minikit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rneasy total rna minikit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm
    Article Snippet: RNASeq and KEGG analysis RNAs for five control and four PKP2-cKO mice at 21 dpi were extracted using RNA-easy Mini kit (Qiagen). .. RNA-Seq library preps were made using the Illumina TruSeq RNA Library Preparation Kit v2 using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50 at the Genome Technology Center at NYUMC.

    Construct:

    Article Title: Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer Cells
    Article Snippet: RNA was harvested after 48 h from transfection by RNA isolation kit (RNeasy Mini Kit, Qiagen 74104). .. Two μg of RNA were polyA+ selected and RNA-Seq libraries were constructed using Illumina TruSeq RNA sample prep kit (TruSeq™ RNA Sample Prep Kit v2-Set B, Illumina, RS-122-2002).

    SYBR Green Assay:

    Article Title: Role of EGFR degradation in cisplatin-induced cytotoxicity in head and neck cancer
    Article Snippet: Total RNA was isolated from UMSCC11B cells using the RNA easy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. Diluted cDNA was used to amplify GAPDH (GAPDH F: 5′ GAG TCA ACG GAT TTG GTC GT 3′ and GAPDH R: 5′ TTG ATT TTG GAG GGA TCT CG 3′) and EGFR (EGFR F: 5′ CTC AGC CAC CCA TAT GTA CC 3′ and EGFR R: 5′ CGT CCA TGT CTT CTT CAT CC 3′) by quantitative reverse-transcription PCR (qRT-PCR) using SYBR green chemistry (Applied Biosystems, Warrington, UK).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Increased Tumor Glycolysis Characterizes Immune Resistance to Adoptive T cell Therapy
    Article Snippet: .. Total RNAs were isolated from either patient formalin-fixed paraffin embedded (FFPE) tumor tissue blocks or patient-derived tumor cell lines using the RNeasy FFPE or RNA mini isolation kits (QIAGEN), respectively, following the manufacturer’s recommendation. .. RNA quality was confirmed using the Agilent 2100 Bioanalyzer.

    Expressing:

    Article Title: Dietary DHA amplifies LXA4 circuits in tissues and lymph node PMN and is protective in immune-driven dry eye disease
    Article Snippet: qPCR mRNA from lymph node PMN was isolated using a RNA Easy Mini Kit (Qiagen Sciences, Germantown, MD, USA) and quantified via spectrophotometry. mRNA was then reverse transcribed using a High-Capacity cDNA Kit (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicates using a StepOne Plus qPCR system (Applied Biosystems) β-actin was used as a reference gene and universal mouse reference RNA was used to calculate relative mRNA expression of our samples as previously described , .

    Article Title: YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells
    Article Snippet: Quantitative PCR Total RNA was isolated from HUVEC or sorted bone ECs using RNA Plus mini kit (Qiagen, Cat#74134) according to the manufacturer’s instructions. .. Quantitative PCR was carried out using gene TaqMan Gene Expression Master Mix (ThermoFisher Scientific, Cat#4369016) and specific Taqman probes human: eukaryotic 18S rRNA (4319413E), VEGFA (Hs00900055_m1, ANGPTL4 (Hs01101127_m1), IGFBP2 (Hs01040719_m1), XBP1 (Hs00231936_m1), CTGF (Hs01026927_g1), CYR61(Hs00998500_g1), YAP1(Hs00902712_g1), WWTR1(Hs00210007_m1), HIF1A(Hs00153153_m1) and mouse probes: Vegfa (Mm00437306_m1), Angptl4 (Mm00480431_m1), Ctgf (Mm01192932_g1), Cyr61 (Mm00487499_g1) from ThermoFisher Scientific (Cat# 4331182) using a C1000 Touch Thermal cycler (BIORAD).

    Article Title: Dietary DHA amplifies LXA4 circuits in tissues and lymph node PMN and is protective in immune-driven dry eye disease
    Article Snippet: mRNA from lymph node PMN was isolated using a RNA Easy Mini Kit (Qiagen Sciences, Germantown, MD, USA) and quantified via spectrophotometry. mRNA was then reverse transcribed using a High-Capacity cDNA Kit (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicates using a StepOne Plus qPCR system (Applied Biosystems) β-actin was used as a reference gene and universal mouse reference RNA was used to calculate relative mRNA expression of our samples as previously described , .

    Hybridization:

    Article Title: Ectromelia virus accumulates less double-stranded RNA compared to vaccinia virus in BS-C-1 cells
    Article Snippet: Total RNA was isolated at the indicated times post-infection using the RNeasy Plus Mini Kit (Qiagen), mixed with an equal volume of Glyoxal loading dye, and heated at 50°C for 1 hour. .. Separation, blotting, and hybridization of RNA were performed with the NorthernMax-Gly Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Transfection:

    Article Title: Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer Cells
    Article Snippet: .. RNA was harvested after 48 h from transfection by RNA isolation kit (RNeasy Mini Kit, Qiagen 74104). .. RNA quality check (RNA integrity number (RIN) > 8) was achieved with Fragment Analyzer (Advanced Analytical Technologies, Inc, Ankeny, IA, USA) and quantified with Qubit (Qubit™ RNA HS Assay Kit, ThermoFisher Scientific, Waltham, MA, USA; ).

    Northern Blot:

    Article Title: Ectromelia virus accumulates less double-stranded RNA compared to vaccinia virus in BS-C-1 cells
    Article Snippet: Paragraph title: Northern Blot ... Total RNA was isolated at the indicated times post-infection using the RNeasy Plus Mini Kit (Qiagen), mixed with an equal volume of Glyoxal loading dye, and heated at 50°C for 1 hour.

    Infection:

    Article Title: Ectromelia virus accumulates less double-stranded RNA compared to vaccinia virus in BS-C-1 cells
    Article Snippet: BS-C-1 cells were infected at the indicated MOI with ECTV or VACV or mock treated. .. Total RNA was isolated at the indicated times post-infection using the RNeasy Plus Mini Kit (Qiagen), mixed with an equal volume of Glyoxal loading dye, and heated at 50°C for 1 hour.

    other:

    Article Title: Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment
    Article Snippet: These methods employ solid phase columns for DNA removal as well, but in contrast to the gDNA eliminator column in the RNeasy Plus Mini kit, those columns are incapable of completely eliminating DNA, that is why their respective protocols include an additional DNase step to work efficiently.

    Article Title: Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment
    Article Snippet: We found that vortexing sputum cells with bashing beads and subsequent loading of the lysate onto the gDNA eliminator spin column of the RNeasy Plus Mini kit was necessary and sufficient to completely eliminate DNA from the samples ( ).

    Polymerase Chain Reaction:

    Article Title: Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm
    Article Snippet: RNASeq and KEGG analysis RNAs for five control and four PKP2-cKO mice at 21 dpi were extracted using RNA-easy Mini kit (Qiagen). .. RNA-Seq library preps were made using the Illumina TruSeq RNA Library Preparation Kit v2 using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50 at the Genome Technology Center at NYUMC.

    Article Title: Role of EGFR degradation in cisplatin-induced cytotoxicity in head and neck cancer
    Article Snippet: Total RNA was isolated from UMSCC11B cells using the RNA easy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. Diluted cDNA was used to amplify GAPDH (GAPDH F: 5′ GAG TCA ACG GAT TTG GTC GT 3′ and GAPDH R: 5′ TTG ATT TTG GAG GGA TCT CG 3′) and EGFR (EGFR F: 5′ CTC AGC CAC CCA TAT GTA CC 3′ and EGFR R: 5′ CGT CCA TGT CTT CTT CAT CC 3′) by quantitative reverse-transcription PCR (qRT-PCR) using SYBR green chemistry (Applied Biosystems, Warrington, UK).

    RNA Sequencing Assay:

    Article Title: Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm
    Article Snippet: RNASeq and KEGG analysis RNAs for five control and four PKP2-cKO mice at 21 dpi were extracted using RNA-easy Mini kit (Qiagen). .. RNA-Seq library preps were made using the Illumina TruSeq RNA Library Preparation Kit v2 using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50 at the Genome Technology Center at NYUMC.

    Article Title: Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer Cells
    Article Snippet: Paragraph title: 4.3. RNA-Seq ... RNA was harvested after 48 h from transfection by RNA isolation kit (RNeasy Mini Kit, Qiagen 74104).

    Article Title: Increased Tumor Glycolysis Characterizes Immune Resistance to Adoptive T cell Therapy
    Article Snippet: Total RNAs were isolated from either patient formalin-fixed paraffin embedded (FFPE) tumor tissue blocks or patient-derived tumor cell lines using the RNeasy FFPE or RNA mini isolation kits (QIAGEN), respectively, following the manufacturer’s recommendation. .. The RNA expression levels in tumor tissues were determined by high-throughput RNA sequencing, as described previously ( ).

    RNA HS Assay:

    Article Title: Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer Cells
    Article Snippet: RNA was harvested after 48 h from transfection by RNA isolation kit (RNeasy Mini Kit, Qiagen 74104). .. RNA quality check (RNA integrity number (RIN) > 8) was achieved with Fragment Analyzer (Advanced Analytical Technologies, Inc, Ankeny, IA, USA) and quantified with Qubit (Qubit™ RNA HS Assay Kit, ThermoFisher Scientific, Waltham, MA, USA; ).

    Isolation:

    Article Title: Dietary DHA amplifies LXA4 circuits in tissues and lymph node PMN and is protective in immune-driven dry eye disease
    Article Snippet: .. qPCR mRNA from lymph node PMN was isolated using a RNA Easy Mini Kit (Qiagen Sciences, Germantown, MD, USA) and quantified via spectrophotometry. mRNA was then reverse transcribed using a High-Capacity cDNA Kit (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicates using a StepOne Plus qPCR system (Applied Biosystems) β-actin was used as a reference gene and universal mouse reference RNA was used to calculate relative mRNA expression of our samples as previously described , .

    Article Title: Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment
    Article Snippet: .. Optimizing RNA isolation using the RNeasy Plus Mini kit The RNeasy Plus Mini kit offers two alternative protocols; the standard protocol provides enrichment of the sample with intact mRNAs by eliminating RNAs shorter than 200 nucleotides; the second protocol is designed for the purification of total RNA. ..

    Article Title: Ectromelia virus accumulates less double-stranded RNA compared to vaccinia virus in BS-C-1 cells
    Article Snippet: .. Total RNA was isolated at the indicated times post-infection using the RNeasy Plus Mini Kit (Qiagen), mixed with an equal volume of Glyoxal loading dye, and heated at 50°C for 1 hour. .. Separation, blotting, and hybridization of RNA were performed with the NorthernMax-Gly Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Article Title: Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer Cells
    Article Snippet: .. RNA was harvested after 48 h from transfection by RNA isolation kit (RNeasy Mini Kit, Qiagen 74104). .. RNA quality check (RNA integrity number (RIN) > 8) was achieved with Fragment Analyzer (Advanced Analytical Technologies, Inc, Ankeny, IA, USA) and quantified with Qubit (Qubit™ RNA HS Assay Kit, ThermoFisher Scientific, Waltham, MA, USA; ).

    Article Title: YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells
    Article Snippet: .. Quantitative PCR Total RNA was isolated from HUVEC or sorted bone ECs using RNA Plus mini kit (Qiagen, Cat#74134) according to the manufacturer’s instructions. .. RNA was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad, Cat#1708890).

    Article Title: Anti-vascular effects of the cytosolic phospholipase A2 inhibitor AVX235 in a patient-derived basal-like breast cancer model
    Article Snippet: .. PGE2 EIA RNA was purified from tumor tissue using a standard RNA isolation kit (Qiagen RNeasy mini kit, Cat. no. 74104; Qiagen, Limburg, The Netherlands). .. Tumor samples of 12 ± 3 mg (mean ± SD) were cut from frozen tumors, added to ice-cold lysis buffer with 10 μM indomethacin and 10 μl/ml β-mercaptoethanol, and homogenized using a Precellys 24 (Bertin Corp., Washington, D.C., USA) at 5200 rpm for 20 s. Homogenized samples were treated according to the kit manual.

    Article Title: Role of EGFR degradation in cisplatin-induced cytotoxicity in head and neck cancer
    Article Snippet: .. Total RNA was isolated from UMSCC11B cells using the RNA easy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. RNA (1μg) was reverse transcribed to cDNA using the High Capacity cDNA Archive kit (Applied Biosystems, Foster City, CA) and purified (Millipore Centrifugal Filter Units, Billerica, MA).

    Article Title: High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos
    Article Snippet: Asai et al. [ ] compared three commonly-used methods: TRIzol® , Aurum Total RNA Mini Kit and Qiagen RNeasy Micro Kit, in combination with preservation reagents TRIzol® or RNAlate r® , to obtain high-quality and large quantities of RNA from the copepod Calanus helgolandicus . .. Their results confirmed that the preservation of copepods in RNAlate r® and the extraction with Qiagen RNeasy Micro Kit were the optimal isolation method for high-quality and quantity of RNA for NGS studies of C . helgolandicus .

    Article Title: Increased Tumor Glycolysis Characterizes Immune Resistance to Adoptive T cell Therapy
    Article Snippet: .. Total RNAs were isolated from either patient formalin-fixed paraffin embedded (FFPE) tumor tissue blocks or patient-derived tumor cell lines using the RNeasy FFPE or RNA mini isolation kits (QIAGEN), respectively, following the manufacturer’s recommendation. .. RNA quality was confirmed using the Agilent 2100 Bioanalyzer.

    Flow Cytometry:

    Article Title: Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer Cells
    Article Snippet: RNA was harvested after 48 h from transfection by RNA isolation kit (RNeasy Mini Kit, Qiagen 74104). .. Paired-end (PE) cluster generation was performed using cBot on Flow Cell v3 (TruSeq PE Cluster Kit v3-cBot-HS, Illumina, PE-401-3001).

    Article Title: Anti-vascular effects of the cytosolic phospholipase A2 inhibitor AVX235 in a patient-derived basal-like breast cancer model
    Article Snippet: PGE2 EIA RNA was purified from tumor tissue using a standard RNA isolation kit (Qiagen RNeasy mini kit, Cat. no. 74104; Qiagen, Limburg, The Netherlands). .. For PGE2 analysis, the flow-through of the ethanolic fraction from the RNA isolation kit was collected and stored at -80 °C and at -20 °C prior to use (less than 3 months at -20 °C).

    Labeling:

    Article Title: Increased Tumor Glycolysis Characterizes Immune Resistance to Adoptive T cell Therapy
    Article Snippet: Total RNAs were isolated from either patient formalin-fixed paraffin embedded (FFPE) tumor tissue blocks or patient-derived tumor cell lines using the RNeasy FFPE or RNA mini isolation kits (QIAGEN), respectively, following the manufacturer’s recommendation. .. For each patient-derived tumor cell line, 40 ug of total RNA was used for the labeling reaction.

    Purification:

    Article Title: Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment
    Article Snippet: .. Optimizing RNA isolation using the RNeasy Plus Mini kit The RNeasy Plus Mini kit offers two alternative protocols; the standard protocol provides enrichment of the sample with intact mRNAs by eliminating RNAs shorter than 200 nucleotides; the second protocol is designed for the purification of total RNA. ..

    Article Title: Anti-vascular effects of the cytosolic phospholipase A2 inhibitor AVX235 in a patient-derived basal-like breast cancer model
    Article Snippet: .. PGE2 EIA RNA was purified from tumor tissue using a standard RNA isolation kit (Qiagen RNeasy mini kit, Cat. no. 74104; Qiagen, Limburg, The Netherlands). .. Tumor samples of 12 ± 3 mg (mean ± SD) were cut from frozen tumors, added to ice-cold lysis buffer with 10 μM indomethacin and 10 μl/ml β-mercaptoethanol, and homogenized using a Precellys 24 (Bertin Corp., Washington, D.C., USA) at 5200 rpm for 20 s. Homogenized samples were treated according to the kit manual.

    Article Title: Role of EGFR degradation in cisplatin-induced cytotoxicity in head and neck cancer
    Article Snippet: Total RNA was isolated from UMSCC11B cells using the RNA easy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. RNA (1μg) was reverse transcribed to cDNA using the High Capacity cDNA Archive kit (Applied Biosystems, Foster City, CA) and purified (Millipore Centrifugal Filter Units, Billerica, MA).

    Sequencing:

    Article Title: Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm
    Article Snippet: RNASeq and KEGG analysis RNAs for five control and four PKP2-cKO mice at 21 dpi were extracted using RNA-easy Mini kit (Qiagen). .. Sequencing results were demultiplexed and converted to FASTQ format using Illumina Bcl2FastQ software.

    Quantitative RT-PCR:

    Article Title: Role of EGFR degradation in cisplatin-induced cytotoxicity in head and neck cancer
    Article Snippet: Total RNA was isolated from UMSCC11B cells using the RNA easy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. Diluted cDNA was used to amplify GAPDH (GAPDH F: 5′ GAG TCA ACG GAT TTG GTC GT 3′ and GAPDH R: 5′ TTG ATT TTG GAG GGA TCT CG 3′) and EGFR (EGFR F: 5′ CTC AGC CAC CCA TAT GTA CC 3′ and EGFR R: 5′ CGT CCA TGT CTT CTT CAT CC 3′) by quantitative reverse-transcription PCR (qRT-PCR) using SYBR green chemistry (Applied Biosystems, Warrington, UK).

    IA:

    Article Title: Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer Cells
    Article Snippet: RNA was harvested after 48 h from transfection by RNA isolation kit (RNeasy Mini Kit, Qiagen 74104). .. RNA quality check (RNA integrity number (RIN) > 8) was achieved with Fragment Analyzer (Advanced Analytical Technologies, Inc, Ankeny, IA, USA) and quantified with Qubit (Qubit™ RNA HS Assay Kit, ThermoFisher Scientific, Waltham, MA, USA; ).

    Sample Prep:

    Article Title: Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer Cells
    Article Snippet: RNA was harvested after 48 h from transfection by RNA isolation kit (RNeasy Mini Kit, Qiagen 74104). .. Two μg of RNA were polyA+ selected and RNA-Seq libraries were constructed using Illumina TruSeq RNA sample prep kit (TruSeq™ RNA Sample Prep Kit v2-Set B, Illumina, RS-122-2002).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells
    Article Snippet: .. Quantitative PCR Total RNA was isolated from HUVEC or sorted bone ECs using RNA Plus mini kit (Qiagen, Cat74134) according to the manufacturer’s instructions. .. RNA was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad, Cat#1708890).

    Article Title: Role of EGFR degradation in cisplatin-induced cytotoxicity in head and neck cancer
    Article Snippet: Total RNA was isolated from UMSCC11B cells using the RNA easy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. Diluted cDNA was used to amplify GAPDH (GAPDH F: 5′ GAG TCA ACG GAT TTG GTC GT 3′ and GAPDH R: 5′ TTG ATT TTG GAG GGA TCT CG 3′) and EGFR (EGFR F: 5′ CTC AGC CAC CCA TAT GTA CC 3′ and EGFR R: 5′ CGT CCA TGT CTT CTT CAT CC 3′) by quantitative reverse-transcription PCR (qRT-PCR) using SYBR green chemistry (Applied Biosystems, Warrington, UK).

    Mouse Assay:

    Article Title: Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm
    Article Snippet: .. RNASeq and KEGG analysis RNAs for five control and four PKP2-cKO mice at 21 dpi were extracted using RNA-easy Mini kit (Qiagen). .. RNA-Seq library preps were made using the Illumina TruSeq RNA Library Preparation Kit v2 using 500 ng of total RNA as input, amplified by 12 cycles of PCR, and run on an Illumina 2500 (v4 chemistry), as single read 50 at the Genome Technology Center at NYUMC.

    Software:

    Article Title: Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm
    Article Snippet: RNASeq and KEGG analysis RNAs for five control and four PKP2-cKO mice at 21 dpi were extracted using RNA-easy Mini kit (Qiagen). .. Sequencing results were demultiplexed and converted to FASTQ format using Illumina Bcl2FastQ software.

    Real-time Polymerase Chain Reaction:

    Article Title: Activation of Melanocortin Receptors MC1 and MC5 Attenuates Retinal Damage in Experimental Diabetic Retinopathy
    Article Snippet: .. Real Time PCR Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, West Sussex, UK), according to the manufacturer's instructions. .. Contaminating DNA was removed from RNA preparations using the Ambion Turbo DNA-free system (Life Technologies, Paisley, UK) using manufacturer's instructions.

    Article Title: Dietary DHA amplifies LXA4 circuits in tissues and lymph node PMN and is protective in immune-driven dry eye disease
    Article Snippet: .. qPCR mRNA from lymph node PMN was isolated using a RNA Easy Mini Kit (Qiagen Sciences, Germantown, MD, USA) and quantified via spectrophotometry. mRNA was then reverse transcribed using a High-Capacity cDNA Kit (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicates using a StepOne Plus qPCR system (Applied Biosystems) β-actin was used as a reference gene and universal mouse reference RNA was used to calculate relative mRNA expression of our samples as previously described , .

    Article Title: YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells
    Article Snippet: .. Quantitative PCR Total RNA was isolated from HUVEC or sorted bone ECs using RNA Plus mini kit (Qiagen, Cat#74134) according to the manufacturer’s instructions. .. RNA was reverse-transcribed using the iScript cDNA synthesis kit (Bio-Rad, Cat#1708890).

    Article Title: Dietary DHA amplifies LXA4 circuits in tissues and lymph node PMN and is protective in immune-driven dry eye disease
    Article Snippet: Paragraph title: qPCR ... mRNA from lymph node PMN was isolated using a RNA Easy Mini Kit (Qiagen Sciences, Germantown, MD, USA) and quantified via spectrophotometry. mRNA was then reverse transcribed using a High-Capacity cDNA Kit (Applied Biosystems, Foster City, CA, USA).

    RNA Expression:

    Article Title: Increased Tumor Glycolysis Characterizes Immune Resistance to Adoptive T cell Therapy
    Article Snippet: Total RNAs were isolated from either patient formalin-fixed paraffin embedded (FFPE) tumor tissue blocks or patient-derived tumor cell lines using the RNeasy FFPE or RNA mini isolation kits (QIAGEN), respectively, following the manufacturer’s recommendation. .. The RNA expression levels in tumor tissues were determined by high-throughput RNA sequencing, as described previously ( ).

    Agarose Gel Electrophoresis:

    Article Title: Ectromelia virus accumulates less double-stranded RNA compared to vaccinia virus in BS-C-1 cells
    Article Snippet: Total RNA was isolated at the indicated times post-infection using the RNeasy Plus Mini Kit (Qiagen), mixed with an equal volume of Glyoxal loading dye, and heated at 50°C for 1 hour. .. Briefly, 5 µg total RNA was separated using a 1.2% agarose gel.

    Electrophoresis:

    Article Title: Role of EGFR degradation in cisplatin-induced cytotoxicity in head and neck cancer
    Article Snippet: Total RNA was isolated from UMSCC11B cells using the RNA easy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. .. The PCR products were resolved by electrophoresis on 1.5% agarose gels and melting curve analysis was carried out to confirm the specificity of the product.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Anti-vascular effects of the cytosolic phospholipase A2 inhibitor AVX235 in a patient-derived basal-like breast cancer model
    Article Snippet: .. PGE2 EIA RNA was purified from tumor tissue using a standard RNA isolation kit (Qiagen RNeasy mini kit, Cat. no. 74104; Qiagen, Limburg, The Netherlands). .. Tumor samples of 12 ± 3 mg (mean ± SD) were cut from frozen tumors, added to ice-cold lysis buffer with 10 μM indomethacin and 10 μl/ml β-mercaptoethanol, and homogenized using a Precellys 24 (Bertin Corp., Washington, D.C., USA) at 5200 rpm for 20 s. Homogenized samples were treated according to the kit manual.

    Next-Generation Sequencing:

    Article Title: High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos
    Article Snippet: Several attempts, mainly for non-well-established model organisms, were recently made to establish methods for high-quality RNA extractions, because NGS represents the only way to address specific ecological and evolutionary questions [ , ]. .. Asai et al. [ ] compared three commonly-used methods: TRIzol® , Aurum Total RNA Mini Kit and Qiagen RNeasy Micro Kit, in combination with preservation reagents TRIzol® or RNAlate r® , to obtain high-quality and large quantities of RNA from the copepod Calanus helgolandicus .

    Preserving:

    Article Title: High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos
    Article Snippet: .. Asai et al. [ ] compared three commonly-used methods: TRIzol® , Aurum Total RNA Mini Kit and Qiagen RNeasy Micro Kit, in combination with preservation reagents TRIzol® or RNAlate r® , to obtain high-quality and large quantities of RNA from the copepod Calanus helgolandicus . .. Their results confirmed that the preservation of copepods in RNAlate r® and the extraction with Qiagen RNeasy Micro Kit were the optimal isolation method for high-quality and quantity of RNA for NGS studies of C . helgolandicus .

    Spectrophotometry:

    Article Title: Dietary DHA amplifies LXA4 circuits in tissues and lymph node PMN and is protective in immune-driven dry eye disease
    Article Snippet: .. qPCR mRNA from lymph node PMN was isolated using a RNA Easy Mini Kit (Qiagen Sciences, Germantown, MD, USA) and quantified via spectrophotometry. mRNA was then reverse transcribed using a High-Capacity cDNA Kit (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicates using a StepOne Plus qPCR system (Applied Biosystems) β-actin was used as a reference gene and universal mouse reference RNA was used to calculate relative mRNA expression of our samples as previously described , .

    Article Title: Dietary DHA amplifies LXA4 circuits in tissues and lymph node PMN and is protective in immune-driven dry eye disease
    Article Snippet: .. mRNA from lymph node PMN was isolated using a RNA Easy Mini Kit (Qiagen Sciences, Germantown, MD, USA) and quantified via spectrophotometry. mRNA was then reverse transcribed using a High-Capacity cDNA Kit (Applied Biosystems, Foster City, CA, USA). .. All samples were run in duplicates using a StepOne Plus qPCR system (Applied Biosystems) β-actin was used as a reference gene and universal mouse reference RNA was used to calculate relative mRNA expression of our samples as previously described , .

    Produced:

    Article Title: High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos
    Article Snippet: The most efficient system to stabilize RNA was the TRIzol method that produced high RNA quality and quantity. .. Asai et al. [ ] compared three commonly-used methods: TRIzol® , Aurum Total RNA Mini Kit and Qiagen RNeasy Micro Kit, in combination with preservation reagents TRIzol® or RNAlate r® , to obtain high-quality and large quantities of RNA from the copepod Calanus helgolandicus .

    Concentration Assay:

    Article Title: Activation of Melanocortin Receptors MC1 and MC5 Attenuates Retinal Damage in Experimental Diabetic Retinopathy
    Article Snippet: Real Time PCR Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, West Sussex, UK), according to the manufacturer's instructions. .. The concentration and purity of the RNA were then analysed using the Nandrop ND-1000 (NanoDrop Technologies, Wilmington, DE).

    High Throughput Screening Assay:

    Article Title: High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos
    Article Snippet: Asai et al. [ ] compared three commonly-used methods: TRIzol® , Aurum Total RNA Mini Kit and Qiagen RNeasy Micro Kit, in combination with preservation reagents TRIzol® or RNAlate r® , to obtain high-quality and large quantities of RNA from the copepod Calanus helgolandicus . .. In conclusion, our results provide for the first time a standard protocol for isolating high-quality RNA from sea urchin plutei for high-throughput NGS studies thereby increasing the resources available for a well-established model organism such as P . lividus .

    Article Title: Increased Tumor Glycolysis Characterizes Immune Resistance to Adoptive T cell Therapy
    Article Snippet: Total RNAs were isolated from either patient formalin-fixed paraffin embedded (FFPE) tumor tissue blocks or patient-derived tumor cell lines using the RNeasy FFPE or RNA mini isolation kits (QIAGEN), respectively, following the manufacturer’s recommendation. .. The RNA expression levels in tumor tissues were determined by high-throughput RNA sequencing, as described previously ( ).

    Lysis:

    Article Title: Anti-vascular effects of the cytosolic phospholipase A2 inhibitor AVX235 in a patient-derived basal-like breast cancer model
    Article Snippet: PGE2 EIA RNA was purified from tumor tissue using a standard RNA isolation kit (Qiagen RNeasy mini kit, Cat. no. 74104; Qiagen, Limburg, The Netherlands). .. Tumor samples of 12 ± 3 mg (mean ± SD) were cut from frozen tumors, added to ice-cold lysis buffer with 10 μM indomethacin and 10 μl/ml β-mercaptoethanol, and homogenized using a Precellys 24 (Bertin Corp., Washington, D.C., USA) at 5200 rpm for 20 s. Homogenized samples were treated according to the kit manual.

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    Qiagen rna extraction kit
    si <t>RNA</t> knockdown of TRPM 7 channel abrogates Ca 2+ influx and I spont in <t>K562</t> cells. (A) detection of TRPM 7 transcripts (398 bp) by conventional RT ‐ PCR . Ten μ mol/L doxycycline ( DXC ) almost completely eliminated TRPM 7 mRNA expression. (B) immunoblots of K562 protein extracts by TRPM 7 antibody. Doxycycline concentration dependently (0.1–10 μ mol/L) reduced TRPM 7 protein expression. The results shown in A and B are representative of three independent experiments. (C) fura‐2 Ca 2+ fluorescence imaging from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing tet ‐inducible TRPM 7‐specific si RNA (si TRPM 7). n = 36 and 26, respectively. (D) density of I spont recorded by conventional whole‐cell recording from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing si TRPM 7. To facilitate the induction of TRPM 7 currents, ATP and Mg 2+ were absent in the patch pipette. The numbers of cells tested are shown in parentheses.
    Rna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 938 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy total rna minikit
    si <t>RNA</t> knockdown of TRPM 7 channel abrogates Ca 2+ influx and I spont in <t>K562</t> cells. (A) detection of TRPM 7 transcripts (398 bp) by conventional RT ‐ PCR . Ten μ mol/L doxycycline ( DXC ) almost completely eliminated TRPM 7 mRNA expression. (B) immunoblots of K562 protein extracts by TRPM 7 antibody. Doxycycline concentration dependently (0.1–10 μ mol/L) reduced TRPM 7 protein expression. The results shown in A and B are representative of three independent experiments. (C) fura‐2 Ca 2+ fluorescence imaging from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing tet ‐inducible TRPM 7‐specific si RNA (si TRPM 7). n = 36 and 26, respectively. (D) density of I spont recorded by conventional whole‐cell recording from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing si TRPM 7. To facilitate the induction of TRPM 7 currents, ATP and Mg 2+ were absent in the patch pipette. The numbers of cells tested are shown in parentheses.
    Rneasy Total Rna Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy total rna minikit/product/Qiagen
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    Qiagen rneasy lipid tissue minikit
    si <t>RNA</t> knockdown of TRPM 7 channel abrogates Ca 2+ influx and I spont in <t>K562</t> cells. (A) detection of TRPM 7 transcripts (398 bp) by conventional RT ‐ PCR . Ten μ mol/L doxycycline ( DXC ) almost completely eliminated TRPM 7 mRNA expression. (B) immunoblots of K562 protein extracts by TRPM 7 antibody. Doxycycline concentration dependently (0.1–10 μ mol/L) reduced TRPM 7 protein expression. The results shown in A and B are representative of three independent experiments. (C) fura‐2 Ca 2+ fluorescence imaging from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing tet ‐inducible TRPM 7‐specific si RNA (si TRPM 7). n = 36 and 26, respectively. (D) density of I spont recorded by conventional whole‐cell recording from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing si TRPM 7. To facilitate the induction of TRPM 7 currents, ATP and Mg 2+ were absent in the patch pipette. The numbers of cells tested are shown in parentheses.
    Rneasy Lipid Tissue Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy lipid tissue minikit/product/Qiagen
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    rneasy lipid tissue minikit - by Bioz Stars, 2020-04
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    si RNA knockdown of TRPM 7 channel abrogates Ca 2+ influx and I spont in K562 cells. (A) detection of TRPM 7 transcripts (398 bp) by conventional RT ‐ PCR . Ten μ mol/L doxycycline ( DXC ) almost completely eliminated TRPM 7 mRNA expression. (B) immunoblots of K562 protein extracts by TRPM 7 antibody. Doxycycline concentration dependently (0.1–10 μ mol/L) reduced TRPM 7 protein expression. The results shown in A and B are representative of three independent experiments. (C) fura‐2 Ca 2+ fluorescence imaging from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing tet ‐inducible TRPM 7‐specific si RNA (si TRPM 7). n = 36 and 26, respectively. (D) density of I spont recorded by conventional whole‐cell recording from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing si TRPM 7. To facilitate the induction of TRPM 7 currents, ATP and Mg 2+ were absent in the patch pipette. The numbers of cells tested are shown in parentheses.

    Journal: Physiological Reports

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562

    doi: 10.14814/phy2.13796

    Figure Lengend Snippet: si RNA knockdown of TRPM 7 channel abrogates Ca 2+ influx and I spont in K562 cells. (A) detection of TRPM 7 transcripts (398 bp) by conventional RT ‐ PCR . Ten μ mol/L doxycycline ( DXC ) almost completely eliminated TRPM 7 mRNA expression. (B) immunoblots of K562 protein extracts by TRPM 7 antibody. Doxycycline concentration dependently (0.1–10 μ mol/L) reduced TRPM 7 protein expression. The results shown in A and B are representative of three independent experiments. (C) fura‐2 Ca 2+ fluorescence imaging from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing tet ‐inducible TRPM 7‐specific si RNA (si TRPM 7). n = 36 and 26, respectively. (D) density of I spont recorded by conventional whole‐cell recording from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing si TRPM 7. To facilitate the induction of TRPM 7 currents, ATP and Mg 2+ were absent in the patch pipette. The numbers of cells tested are shown in parentheses.

    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Concentration Assay, Fluorescence, Imaging, Stable Transfection, Transferring

    K562 cell growth is dependent on Ca 2+ influx mediated by TRPM 7 channel. (A) Dependency of K562 cell growth on extracellular Ca 2+ concentration evaluated at 72 h after culture. Open and filled circles indicate K562 cells untreated [ DXC (‐)] or treated with 10 μ mol/L doxycycline [ DXC (+)], respectively. (B) time courses of K562 cell growth under various conditions, i.e. DXC (−) (no doxycycline and normal Ca 2+ in RPMI medium), DXC (−)0Ca 2+ (no doxycycline and Ca 2+ free in the medium), DXC (+) (10 μ mol/L doxycycline and normal Ca 2+ in the medium) and DXC (+)0Ca 2+ (10 μ mol/L doxycycline and Ca 2+ free in the medium). In both types of experiments (A and B), K562 cells stably expressing tet ‐inducible TRPM 7‐si RNA (si TRPM 7) were used. n = 5 for each condition. * P

    Journal: Physiological Reports

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562

    doi: 10.14814/phy2.13796

    Figure Lengend Snippet: K562 cell growth is dependent on Ca 2+ influx mediated by TRPM 7 channel. (A) Dependency of K562 cell growth on extracellular Ca 2+ concentration evaluated at 72 h after culture. Open and filled circles indicate K562 cells untreated [ DXC (‐)] or treated with 10 μ mol/L doxycycline [ DXC (+)], respectively. (B) time courses of K562 cell growth under various conditions, i.e. DXC (−) (no doxycycline and normal Ca 2+ in RPMI medium), DXC (−)0Ca 2+ (no doxycycline and Ca 2+ free in the medium), DXC (+) (10 μ mol/L doxycycline and normal Ca 2+ in the medium) and DXC (+)0Ca 2+ (10 μ mol/L doxycycline and Ca 2+ free in the medium). In both types of experiments (A and B), K562 cells stably expressing tet ‐inducible TRPM 7‐si RNA (si TRPM 7) were used. n = 5 for each condition. * P

    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA.

    Techniques: Concentration Assay, Stable Transfection, Expressing

    Erythroid differentiation of K562 cell by hemin is regulated by Ca 2+ influx mediated by TRPM 7. (A) representative RT ‐ PCR experiments showing hemin‐induced γ ‐globin synthesis in K562 cells untreated (control) and treated with 10 μ mol/L FTY 720 (+ FTY ), 1 mmol/L EGTA (+ EGTA ) or 10 μ mol/L doxycycline (+dxc). In all conditions, K562 cells stably expressing tet ‐inducible TRPM 7‐si RNA (si TRPM 7) were used. The expression of γ ‐globin mRNA was greatly decreased by these procedues, while that of GAPDH stayed almost constant. (B and C) statistical evaluation of pooled data from experiments as shown in A: 10 μ mol/L FTY 720 and 1 mmol/L EGTA (B): si TRPM 7 (C). (D and E) effects of 10 μ mol/L FTY 720 ( FTY ), 1 mmol/L EGTA ( EGTA ) or 10 μ mol/L doxycycline (+dxc) on hemoglobin synthesis at rest or 3 days after hemin treatment in K562‐cells stably expressing si TRPM 7. n = 5 for each condition. * P

    Journal: Physiological Reports

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562

    doi: 10.14814/phy2.13796

    Figure Lengend Snippet: Erythroid differentiation of K562 cell by hemin is regulated by Ca 2+ influx mediated by TRPM 7. (A) representative RT ‐ PCR experiments showing hemin‐induced γ ‐globin synthesis in K562 cells untreated (control) and treated with 10 μ mol/L FTY 720 (+ FTY ), 1 mmol/L EGTA (+ EGTA ) or 10 μ mol/L doxycycline (+dxc). In all conditions, K562 cells stably expressing tet ‐inducible TRPM 7‐si RNA (si TRPM 7) were used. The expression of γ ‐globin mRNA was greatly decreased by these procedues, while that of GAPDH stayed almost constant. (B and C) statistical evaluation of pooled data from experiments as shown in A: 10 μ mol/L FTY 720 and 1 mmol/L EGTA (B): si TRPM 7 (C). (D and E) effects of 10 μ mol/L FTY 720 ( FTY ), 1 mmol/L EGTA ( EGTA ) or 10 μ mol/L doxycycline (+dxc) on hemoglobin synthesis at rest or 3 days after hemin treatment in K562‐cells stably expressing si TRPM 7. n = 5 for each condition. * P

    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Stable Transfection, Expressing