rneasy plus mini kit  (Qiagen)


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  • 99

    Structured Review

    Qiagen rneasy plus mini kit
    Rneasy Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plus mini kit/product/Qiagen
    Average 99 stars, based on 841 article reviews
    Price from $9.99 to $1999.99
    rneasy plus mini kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: A population of gut epithelial enterochromaffin cells is mechanosensitive and requires Piezo2 to convert force into serotonin release
    Article Snippet: The remaining supernatant was divided and incubated overnight with either a mouse monoclonal anti-HA antibody (901513; Biolegend) or Mouse IgG (MABF1081Z; Millipore) coupled to Protein G magnetic beads (Dynabeads; Invitrogen). .. RLT buffer (RNeasy Plus Mini Kit; Qiagen) supplemented with β-mercaptoethanol was added to each sample and then vortexed for 30-s RNA isolation was then performed on all samples using the RNeasy Plus Mini Kit (Qiagen).

    Isolation:

    Article Title: A population of gut epithelial enterochromaffin cells is mechanosensitive and requires Piezo2 to convert force into serotonin release
    Article Snippet: .. RLT buffer (RNeasy Plus Mini Kit; Qiagen) supplemented with β-mercaptoethanol was added to each sample and then vortexed for 30-s RNA isolation was then performed on all samples using the RNeasy Plus Mini Kit (Qiagen). ..

    Homogenization:

    Article Title: A population of gut epithelial enterochromaffin cells is mechanosensitive and requires Piezo2 to convert force into serotonin release
    Article Snippet: Samples were prepared by homogenizing the epithelial tissue pellet with homogenization buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM Mg Cl2 , 1% Nonidet P-40, 1 mM DTT, 200 U/mL Promega RNasin, 1 mg/mL heparin, 100 µg/mL cycloheximide, Sigma protease inhibitor mixture). .. RLT buffer (RNeasy Plus Mini Kit; Qiagen) supplemented with β-mercaptoethanol was added to each sample and then vortexed for 30-s RNA isolation was then performed on all samples using the RNeasy Plus Mini Kit (Qiagen).

    Incubation:

    Article Title: A population of gut epithelial enterochromaffin cells is mechanosensitive and requires Piezo2 to convert force into serotonin release
    Article Snippet: After overnight incubation, the beads were washed three times for a total of 30 min in a high-salt buffer (50 mM Tris, pH 7.5, 300 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100 µg/mL cycloheximide). .. RLT buffer (RNeasy Plus Mini Kit; Qiagen) supplemented with β-mercaptoethanol was added to each sample and then vortexed for 30-s RNA isolation was then performed on all samples using the RNeasy Plus Mini Kit (Qiagen).

    Protease Inhibitor:

    Article Title: A population of gut epithelial enterochromaffin cells is mechanosensitive and requires Piezo2 to convert force into serotonin release
    Article Snippet: Samples were prepared by homogenizing the epithelial tissue pellet with homogenization buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM Mg Cl2 , 1% Nonidet P-40, 1 mM DTT, 200 U/mL Promega RNasin, 1 mg/mL heparin, 100 µg/mL cycloheximide, Sigma protease inhibitor mixture). .. RLT buffer (RNeasy Plus Mini Kit; Qiagen) supplemented with β-mercaptoethanol was added to each sample and then vortexed for 30-s RNA isolation was then performed on all samples using the RNeasy Plus Mini Kit (Qiagen).

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  • 90
    Qiagen rneasy plus mini kit
    Rneasy Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plus mini kit/product/Qiagen
    Average 90 stars, based on 841 article reviews
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    Qiagen buffer rlt plus
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