Structured Review

Qiagen rneasy plus kit
Rneasy Plus Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rneasy plus kit/product/Qiagen
Average 96 stars, based on 29 article reviews
Price from $9.99 to $1999.99
rneasy plus kit - by Bioz Stars, 2020-05
96/100 stars

Images

Related Articles

Isolation:

Article Title: Ectopic protein interactions within BRD4–chromatin complexes drive oncogenic megadomain formation in NUT midline carcinoma
Article Snippet: .. RNA was extracted from live cultured 24335 cells using the RNeasy Plus Kit (Qiagen) followed by the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). rRNA removal was performed using the Ribo-Zero Kit (Epicentre) following the manufacturer’s instructions. .. The library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer’s instructions and sequenced at the Partners HealthCare Personalized Medicine/Genetics and Genomics core facility ( ) using the HiSeq 2500 (Illumina).

Cell Culture:

Article Title: Ectopic protein interactions within BRD4–chromatin complexes drive oncogenic megadomain formation in NUT midline carcinoma
Article Snippet: .. RNA was extracted from live cultured 24335 cells using the RNeasy Plus Kit (Qiagen) followed by the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). rRNA removal was performed using the Ribo-Zero Kit (Epicentre) following the manufacturer’s instructions. .. The library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer’s instructions and sequenced at the Partners HealthCare Personalized Medicine/Genetics and Genomics core facility ( ) using the HiSeq 2500 (Illumina).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen rneasy kit
    2′- O methylation of internal adenosine. (A) Incorporation of 3 H-methyl into polyA. Homopolymer RNAs (1 µg) were incubated with 2 µg of DENV-4 MTase in the presence of [ 3 H-methyl]-SAM. After the methylation reaction, the un-incorporated [ 3 H-methyl]-SAM was removed by <t>RNeasy</t> kit. The amount of 3 H-methyl incorporation was measured by a MicroBeta counting. (B) SPA-based methylation analysis of oligo (A) 12 , (Am) 12 , and (m 6 ,m 6 A) 12 . All three <t>RNA</t> oligos were 3′-end biotinylated to facilitate SPA analysis. Am indicates that the 2′-OH of adenosine is methylated. m 6 ,m 6 A indicates that the amino N 6 position of adenosine is double methylated. (C) SPA-based methylation analysis of DENV-1 RNA. pppA-RNAs, representing the 5′ 211 nt of DENV-1 genome, were in vitro transcribed using biotinylated-CTP plus unmodified ATP, 2′- O -methyladenosine triphosphate (AmTP), or N 6 methyl adenosine triphosphate (m 6 ATP). The transcription reactions generated pppA-RNA, ppp(Am)-RNA, and ppp(m 6 A)RNA, respectively. The RNAs were then subjected to SPA-based internal methylation analysis. Average results and standard deviations from three independent experiments are presented.
    Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3876 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy kit/product/Qiagen
    Average 99 stars, based on 3876 article reviews
    Price from $9.99 to $1999.99
    rneasy kit - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Qiagen enscs
    Effect on gene expression of Bmi1 overexpression in <t>eNSCs</t> compared to aNSCs. ( A ) Comparison of transcript levels measured by <t>RNA-seq</t> (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P
    Enscs, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enscs/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    enscs - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    2′- O methylation of internal adenosine. (A) Incorporation of 3 H-methyl into polyA. Homopolymer RNAs (1 µg) were incubated with 2 µg of DENV-4 MTase in the presence of [ 3 H-methyl]-SAM. After the methylation reaction, the un-incorporated [ 3 H-methyl]-SAM was removed by RNeasy kit. The amount of 3 H-methyl incorporation was measured by a MicroBeta counting. (B) SPA-based methylation analysis of oligo (A) 12 , (Am) 12 , and (m 6 ,m 6 A) 12 . All three RNA oligos were 3′-end biotinylated to facilitate SPA analysis. Am indicates that the 2′-OH of adenosine is methylated. m 6 ,m 6 A indicates that the amino N 6 position of adenosine is double methylated. (C) SPA-based methylation analysis of DENV-1 RNA. pppA-RNAs, representing the 5′ 211 nt of DENV-1 genome, were in vitro transcribed using biotinylated-CTP plus unmodified ATP, 2′- O -methyladenosine triphosphate (AmTP), or N 6 methyl adenosine triphosphate (m 6 ATP). The transcription reactions generated pppA-RNA, ppp(Am)-RNA, and ppp(m 6 A)RNA, respectively. The RNAs were then subjected to SPA-based internal methylation analysis. Average results and standard deviations from three independent experiments are presented.

    Journal: PLoS Pathogens

    Article Title: 2?-O Methylation of Internal Adenosine by Flavivirus NS5 Methyltransferase

    doi: 10.1371/journal.ppat.1002642

    Figure Lengend Snippet: 2′- O methylation of internal adenosine. (A) Incorporation of 3 H-methyl into polyA. Homopolymer RNAs (1 µg) were incubated with 2 µg of DENV-4 MTase in the presence of [ 3 H-methyl]-SAM. After the methylation reaction, the un-incorporated [ 3 H-methyl]-SAM was removed by RNeasy kit. The amount of 3 H-methyl incorporation was measured by a MicroBeta counting. (B) SPA-based methylation analysis of oligo (A) 12 , (Am) 12 , and (m 6 ,m 6 A) 12 . All three RNA oligos were 3′-end biotinylated to facilitate SPA analysis. Am indicates that the 2′-OH of adenosine is methylated. m 6 ,m 6 A indicates that the amino N 6 position of adenosine is double methylated. (C) SPA-based methylation analysis of DENV-1 RNA. pppA-RNAs, representing the 5′ 211 nt of DENV-1 genome, were in vitro transcribed using biotinylated-CTP plus unmodified ATP, 2′- O -methyladenosine triphosphate (AmTP), or N 6 methyl adenosine triphosphate (m 6 ATP). The transcription reactions generated pppA-RNA, ppp(Am)-RNA, and ppp(m 6 A)RNA, respectively. The RNAs were then subjected to SPA-based internal methylation analysis. Average results and standard deviations from three independent experiments are presented.

    Article Snippet: For each time point, total cellular RNA was extracted using RNeasy kit (Qiagen).

    Techniques: Methylation, Incubation, In Vitro, Generated

    Quantitative RT-PCR analysis of PAI-1 mRNA levels in patients. The total RNA was harvested from the peripheral blood mononuclear cells using an RNeasy kit according to the manufacturer’s instructions. The RT-PCR experiments were repeated at least 3 times. RNA was reverse transcribed into cDNA using random primers in a Reverse Transcription II system according to the manufacturer’s instructions. The expression of PAI-1 mRNA was quantified by quantitative PCR using an ABI Prism Sequence Detection System. Template-negative and RT-negative conditions were used as controls. Amplification of the endogenous GAPDH cDNA was monitored. The levels (mean value) of PAI-1 transcripts in patients were calculated. RT-PCR, reverse-transcription-PCR, PAI-1, plasminogen activator inhibitor-1.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Therapeutic effects of calcium dobesilate on diabetic nephropathy mediated through reduction of expression of PAI-1

    doi: 10.3892/etm.2012.755

    Figure Lengend Snippet: Quantitative RT-PCR analysis of PAI-1 mRNA levels in patients. The total RNA was harvested from the peripheral blood mononuclear cells using an RNeasy kit according to the manufacturer’s instructions. The RT-PCR experiments were repeated at least 3 times. RNA was reverse transcribed into cDNA using random primers in a Reverse Transcription II system according to the manufacturer’s instructions. The expression of PAI-1 mRNA was quantified by quantitative PCR using an ABI Prism Sequence Detection System. Template-negative and RT-negative conditions were used as controls. Amplification of the endogenous GAPDH cDNA was monitored. The levels (mean value) of PAI-1 transcripts in patients were calculated. RT-PCR, reverse-transcription-PCR, PAI-1, plasminogen activator inhibitor-1.

    Article Snippet: The total RNA was harvested from peripheral blood mononuclear cells using the RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction, Sequencing, Amplification, Polymerase Chain Reaction

    SPP1 expression is up-regulated in glioma initiating cells ( A ) Representative examples of dot plots and histograms of Rhod 123(–) subpopulations from 5 glioma cell lines were acquired by flow cytometry. Glioma cells (1 × 10 7 ) were trypsinized and incubated with 0.1 μg/ml Rhod123 for 20 min in 37°C. After washing the cells were placed in 37°C for 90 min for Rhod123 exclusion in a dark compartment. Cells kept on ice to inhibit exclusion of Rhod123 were used as a positive control for gating in flow cytometry. Fractions of Rhod123(+) and Rhod123(–) cells were sorted using FACS Aria and a left panel shows a gating strategy. ( B ) Analysis of SPP1, OCT3/4 and NANOG gene expression in Rhod 123(–) subpopulations sorted from four human glioma cell lines. Total RNA was isolated from sorted cells using Qiagen RNeasy kit and the levels of SPP1, NANOG and OCT3/4 mRNA were determined by qPCR in Rhod 123(+) and Rhod 123(–) subpopulations; their expression in Rhod 123(+) subpopulations was taken as 1 (a red line). P values were calculated with a t-test and considered significant when *P ≤ 0.05 and * *P ≤ 0.01. ( C ) Quantification of the expression of Spp1, Oct3/4 and Nanog in Rhod 123(–) subpopulations isolated from rat C6 glioma cells versus their levels in Rhod 123(+) subpopulations taken as 1 (a red line); n = 3.

    Journal: Oncotarget

    Article Title: The embryonic type of SPP1 transcriptional regulation is re-activated in glioblastoma

    doi: 10.18632/oncotarget.14092

    Figure Lengend Snippet: SPP1 expression is up-regulated in glioma initiating cells ( A ) Representative examples of dot plots and histograms of Rhod 123(–) subpopulations from 5 glioma cell lines were acquired by flow cytometry. Glioma cells (1 × 10 7 ) were trypsinized and incubated with 0.1 μg/ml Rhod123 for 20 min in 37°C. After washing the cells were placed in 37°C for 90 min for Rhod123 exclusion in a dark compartment. Cells kept on ice to inhibit exclusion of Rhod123 were used as a positive control for gating in flow cytometry. Fractions of Rhod123(+) and Rhod123(–) cells were sorted using FACS Aria and a left panel shows a gating strategy. ( B ) Analysis of SPP1, OCT3/4 and NANOG gene expression in Rhod 123(–) subpopulations sorted from four human glioma cell lines. Total RNA was isolated from sorted cells using Qiagen RNeasy kit and the levels of SPP1, NANOG and OCT3/4 mRNA were determined by qPCR in Rhod 123(+) and Rhod 123(–) subpopulations; their expression in Rhod 123(+) subpopulations was taken as 1 (a red line). P values were calculated with a t-test and considered significant when *P ≤ 0.05 and * *P ≤ 0.01. ( C ) Quantification of the expression of Spp1, Oct3/4 and Nanog in Rhod 123(–) subpopulations isolated from rat C6 glioma cells versus their levels in Rhod 123(+) subpopulations taken as 1 (a red line); n = 3.

    Article Snippet: Real- time PCR Total RNA was isolated from glioma cells using Qiagen RNeasy kit, 1 μg was used as a template.

    Techniques: Expressing, Flow Cytometry, Cytometry, Incubation, Positive Control, FACS, Isolation, Real-time Polymerase Chain Reaction

    Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Journal: Scientific Reports

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    doi: 10.1038/s41598-018-25921-8

    Figure Lengend Snippet: Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Article Snippet: High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples.

    Techniques: Expressing, Over Expression, RNA Sequencing Assay, Plasmid Preparation

    Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Journal: Scientific Reports

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    doi: 10.1038/s41598-018-25921-8

    Figure Lengend Snippet: Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Article Snippet: High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples.

    Techniques: Expressing, Over Expression, RNA Sequencing Assay