rneasy plant mini kit  (Qiagen)


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    Structured Review

    Qiagen rneasy plant mini kit
    Rneasy Plant Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant mini kit/product/Qiagen
    Average 99 stars, based on 1665 article reviews
    Price from $9.99 to $1999.99
    rneasy plant mini kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: .. RNA extraction and library preparation Total RNA was extracted from 100 mg leaf samples using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. The quality of RNA was assessed using denaturing agarose gel stained with gelred (Biotium, USA) and quantified using a Nanodrop 2000 (Thermo Fisher Scientific, MA, USA).

    Article Title: Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement
    Article Snippet: .. RNA EXTRACTION AND RT-PCR ANALYSIS Total RNA was extracted from 100 mg young leaf tissue of 10 transgenic lines and non-transgenic control plants using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. The quantity and quality (A260/230 and A260/280 ) of total RNA were determined using the Nanodrop 2000.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement
    Article Snippet: .. RNA EXTRACTION AND RT-PCR ANALYSIS Total RNA was extracted from 100 mg young leaf tissue of 10 transgenic lines and non-transgenic control plants using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. The quantity and quality (A260/230 and A260/280 ) of total RNA were determined using the Nanodrop 2000.

    Agarose Gel Electrophoresis:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: RNA extraction and library preparation Total RNA was extracted from 100 mg leaf samples using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. The quality of RNA was assessed using denaturing agarose gel stained with gelred (Biotium, USA) and quantified using a Nanodrop 2000 (Thermo Fisher Scientific, MA, USA).

    In Vitro:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: One month-old in vitro rooted plantlets were artificially inoculated with 100 μl of bacterial culture (108 cfu ml−1 ) of Xcm. .. The inoculated leaf samples were collected at 0, 12 hpi, 48 hpi and RNA were isolated using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen).

    Synthesized:

    Article Title: Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement
    Article Snippet: RNA EXTRACTION AND RT-PCR ANALYSIS Total RNA was extracted from 100 mg young leaf tissue of 10 transgenic lines and non-transgenic control plants using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. Complementary DNA (cDNA) was synthesized using 1 μg of total RNA and reverse transcriptase of the Maxima H Minus First Strand cDNA synthesis kit with oligoDT primers (Thermo scientific).

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: The inoculated leaf samples were collected at 0, 12 hpi, 48 hpi and RNA were isolated using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. First strand cDNA was synthesized using reverse transcriptase of the Maxima H Minus First Strand cDNA synthesis kit with oligo DT primers (Thermo scientific).

    Isolation:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: .. The inoculated leaf samples were collected at 0, 12 hpi, 48 hpi and RNA were isolated using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. First strand cDNA was synthesized using reverse transcriptase of the Maxima H Minus First Strand cDNA synthesis kit with oligo DT primers (Thermo scientific).

    Magnetic Beads:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: RNA extraction and library preparation Total RNA was extracted from 100 mg leaf samples using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. Poly (A) mRNA was obtained from 1 µg of the total RNA using poly-T oligos attached to magnetic beads.

    Quantitative RT-PCR:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: Paragraph title: Quantitative RT-PCR validation ... The inoculated leaf samples were collected at 0, 12 hpi, 48 hpi and RNA were isolated using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen).

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: The primers were designed using the PrimerQuest Tool (Integrated DNA Technologies, Iowa, USA) using the 2 primers, intercalating dyes qPCR design type. .. The inoculated leaf samples were collected at 0, 12 hpi, 48 hpi and RNA were isolated using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen).

    Transgenic Assay:

    Article Title: Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement
    Article Snippet: .. RNA EXTRACTION AND RT-PCR ANALYSIS Total RNA was extracted from 100 mg young leaf tissue of 10 transgenic lines and non-transgenic control plants using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. The quantity and quality (A260/230 and A260/280 ) of total RNA were determined using the Nanodrop 2000.

    Staining:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: RNA extraction and library preparation Total RNA was extracted from 100 mg leaf samples using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. The quality of RNA was assessed using denaturing agarose gel stained with gelred (Biotium, USA) and quantified using a Nanodrop 2000 (Thermo Fisher Scientific, MA, USA).

    Polymerase Chain Reaction:

    Article Title: Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement
    Article Snippet: RNA EXTRACTION AND RT-PCR ANALYSIS Total RNA was extracted from 100 mg young leaf tissue of 10 transgenic lines and non-transgenic control plants using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. RNA was checked with PCR for absence of genomic DNA.

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: The inoculated leaf samples were collected at 0, 12 hpi, 48 hpi and RNA were isolated using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. The primer specificity for each gene was confirmed by PCR and melting curve in qRT-PCR.

    RNA Sequencing Assay:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: Quantitative RT-PCR validation To verify the results of RNA-Seq analysis, qRT-PCR analysis was performed with 30 selected DEGs using gene specific primers (Supplementary Table ). .. The inoculated leaf samples were collected at 0, 12 hpi, 48 hpi and RNA were isolated using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen).

    Sample Prep:

    Article Title: Molecular Basis of Disease Resistance in Banana Progenitor Musa balbisiana against Xanthomonas campestris pv. musacearum
    Article Snippet: RNA extraction and library preparation Total RNA was extracted from 100 mg leaf samples using the RNeasy plant mini kit (Qiagen, GmbH, Hilden, Germany) and treated with DNase (RNeasy Plant Mini kit, Qiagen). .. A total of 30 cDNA libraries were prepared in each of the two treatments [three biological replicates at each of the three time points (0, 12 hpi and 48 hpi) per genotype inoculated with bacterial culture and two time points (12 hpi, 48 hpi) inoculated with sterile water] using Illumina TruSeq RNA Sample Preparation Kit v2.

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  • About
  • News
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  • Team
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  • Bioz Stars
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  • 90
    Qiagen rneasy plant mini kit
    Rneasy Plant Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant mini kit/product/Qiagen
    Average 90 stars, based on 1665 article reviews
    Price from $9.99 to $1999.99
    rneasy plant mini kit - by Bioz Stars, 2020-01
    90/100 stars
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