rneasy minikit  (Qiagen)

 
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    Name:
    RNeasy Mini Kit
    Description:
    For purification of up to 100 µg total RNA from cells tissues and yeast Kit contents Qiagen RNeasy Mini Kit 50 preps 0 5 to 30mg Sample 30 to 100L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR For Purification of Up to 100g Total RNA from Cells Tissues and Yeast Includes 250 RNeasy Mini Spin Columns Collection Tubes 1 5mL and 2mL RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from small amounts of starting material No phenol chloroform extraction no CsCl gradients no LiCl or ethanol precipitatio
    Catalog Number:
    74104
    Price:
    314
    Category:
    RNeasy Mini Kit
    Buy from Supplier


    Structured Review

    Qiagen rneasy minikit
    RNeasy Mini Kit
    For purification of up to 100 µg total RNA from cells tissues and yeast Kit contents Qiagen RNeasy Mini Kit 50 preps 0 5 to 30mg Sample 30 to 100L Elution Volume Tissue Cells Sample Total RNA Purification Silica Technology Spin Column Format Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR For Purification of Up to 100g Total RNA from Cells Tissues and Yeast Includes 250 RNeasy Mini Spin Columns Collection Tubes 1 5mL and 2mL RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from small amounts of starting material No phenol chloroform extraction no CsCl gradients no LiCl or ethanol precipitatio
    https://www.bioz.com/result/rneasy minikit/product/Qiagen
    Average 99 stars, based on 2619 article reviews
    Price from $9.99 to $1999.99
    rneasy minikit - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game"

    Article Title: Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game

    Journal: Journal of Virology

    doi: 10.1128/JVI.01464-15

    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.
    Figure Legend Snippet: Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.

    Techniques Used: Real-time Polymerase Chain Reaction, Transfection, Isolation

    Related Articles

    RNA Extraction:

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

    Article Title: NF-κB promotes the stem-like properties of leukemia cells by activation of LIN28B
    Article Snippet: .. RNA extraction and qRT-PCR RNeasy Mini Kit (Qiagen Hilden, Germany) was purchased for RNA extraction. .. In brief, cDNA was synthesized by using the i-Script™ Reverse Transcription Supermix (Biorad, Hercules, CA, United States) from 1 µg of total RNA.

    Article Title: Astrocyte-elevated gene-1 confers resistance to pemetrexed in non-small cell lung cancer by upregulating thymidylate synthase expression
    Article Snippet: .. RNA extraction and quantitative real-time PCR Total RNA was extracted using a Qiagen mRNA easy mini kit (Qiagen). .. First-strand complementary DNA synthesis was performed from 2 μg of total RNA by using Invitrogen SuperScript Reverse Transcriptase (Thermo Fisher Scientific, Carlsbad, CA, USA).

    Filtration:

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

    Homogenization:

    Article Title: Monkeypox virus induces the synthesis of less dsRNA than vaccinia virus, and is more resistant to the anti-poxvirus drug, IBT, than vaccinia virus
    Article Snippet: .. Total RNA was extracted with the RNeasy Mini Kit, using QiaShredder homogenization and in-column DNase treatment as described by the manufacturer (Qiagen). .. An equal volume of total RNA (2 μL) was diluted in ddH2 0 (198 μL).

    Isolation:

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

    Article Title: PTK787/ZK22258 attenuates stellate cell activation and hepatic fibrosis in vivo by inhibiting VEGF signaling
    Article Snippet: .. Total RNA was isolated from liver tissues and HSCs using RNeasy Mini kit (Qiagen Inc., Hilden, Germany), according to the manufacturer’s instruction. .. For cDNA synthesis, Taqman reverse transcription reagents were used as described in the manufacture’s protocol (PE Applied Biosystems, Foster City, CA, USA).

    Quantitative RT-PCR:

    Article Title: NF-κB promotes the stem-like properties of leukemia cells by activation of LIN28B
    Article Snippet: .. RNA extraction and qRT-PCR RNeasy Mini Kit (Qiagen Hilden, Germany) was purchased for RNA extraction. .. In brief, cDNA was synthesized by using the i-Script™ Reverse Transcription Supermix (Biorad, Hercules, CA, United States) from 1 µg of total RNA.

    Article Title: PDCD6 is an independent predictor of progression free survival in epithelial ovarian cancer
    Article Snippet: .. Quantitative real-time polymerase chain reaction (qRT-PCR) RNeasy Mini Kits (QIAGEN Inc., Valencia, CA) were used to extract total RNA from cells and tissue. .. RNA (5 ng) was reverse transcribed to cDNA using a SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen Corp., Carlsbad, CA).

    Purification:

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Astrocyte-elevated gene-1 confers resistance to pemetrexed in non-small cell lung cancer by upregulating thymidylate synthase expression
    Article Snippet: .. RNA extraction and quantitative real-time PCR Total RNA was extracted using a Qiagen mRNA easy mini kit (Qiagen). .. First-strand complementary DNA synthesis was performed from 2 μg of total RNA by using Invitrogen SuperScript Reverse Transcriptase (Thermo Fisher Scientific, Carlsbad, CA, USA).

    Article Title: PDCD6 is an independent predictor of progression free survival in epithelial ovarian cancer
    Article Snippet: .. Quantitative real-time polymerase chain reaction (qRT-PCR) RNeasy Mini Kits (QIAGEN Inc., Valencia, CA) were used to extract total RNA from cells and tissue. .. RNA (5 ng) was reverse transcribed to cDNA using a SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen Corp., Carlsbad, CA).

    Sequencing:

    Article Title: Glycosylation is an Androgen-Regulated Process Essential for Prostate Cancer Cell Viability
    Article Snippet: .. 2.1 RNA-Seq and Analysis For RNA-Seq of LNCaP cells: RNA was prepared for sequencing using a RNA Easy Kit (Qiagen 74104). .. All RNA samples were DNase I treated using a DNA-free kit (Invitrogen) and stored at − 80 °C prior to RNA quality control check using 2100 Agilent Bioanalyser and mRNA library prep using TruSeq mRNA library kit (Illumina).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Comparison of RNA extraction methods to augment the sensitivity for the differentiation of vesicular stomatitis virus Indiana1 and New Jersey
    Article Snippet: .. In summary, the results of the study showed that the use of Qiagen RNeasy mini kit in addition to the one‐step RT‐PCR kit (Qiagen, Valencia, CA) as a simple, practicable, and reliable tool for rapid, highly sensitive, and specific differential diagnosis of VSV‐IND and VSV‐NJ in cell lysate and spiked samples. .. In summary, the results of the study showed that the use of Qiagen RNeasy mini kit in addition to the one‐step RT‐PCR kit (Qiagen, Valencia, CA) as a simple, practicable, and reliable tool for rapid, highly sensitive, and specific differential diagnosis of VSV‐IND and VSV‐NJ in cell lysate and spiked samples.

    RNA Sequencing Assay:

    Article Title: Glycosylation is an Androgen-Regulated Process Essential for Prostate Cancer Cell Viability
    Article Snippet: .. 2.1 RNA-Seq and Analysis For RNA-Seq of LNCaP cells: RNA was prepared for sequencing using a RNA Easy Kit (Qiagen 74104). .. All RNA samples were DNase I treated using a DNA-free kit (Invitrogen) and stored at − 80 °C prior to RNA quality control check using 2100 Agilent Bioanalyser and mRNA library prep using TruSeq mRNA library kit (Illumina).

    Molecular Weight:

    Article Title: Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles
    Article Snippet: .. Total RNA Extraction and Small RNAs Enrichment Protocols Total RNA was extracted using three different methods: an acid phenol/guanidine isothiocyanate solution (TRIzol Reagent, Invitrogen), a glass-fiber filtration protocol (MirVana miRNA Isolation Kit, Ambion) that provides also a procedure to isolate and enrich low molecular weight (LMW) RNAs from higher molecular weight (HMW) RNAs, and another common glass-fiber purification protocol (RNEasy Mini Kit, Qiagen). ..

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  • 99
    Qiagen rneasy minikit
    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen <t>RNeasy</t> <t>minikit.</t>
    Rneasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy minikit/product/Qiagen
    Average 99 stars, based on 2619 article reviews
    Price from $9.99 to $1999.99
    rneasy minikit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Qiagen rneasy plant minikit
    Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the <t>RNeasy</t> plant <t>minikit</t> and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.
    Rneasy Plant Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant minikit/product/Qiagen
    Average 99 stars, based on 95 article reviews
    Price from $9.99 to $1999.99
    rneasy plant minikit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.

    Journal: Journal of Virology

    Article Title: Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game

    doi: 10.1128/JVI.01464-15

    Figure Lengend Snippet: Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.

    Article Snippet: Forty-eight hours after siRNA transfection, total RNA was isolated from mock-infected and transfected HEp-2C cells and Vero cells using an RNeasy minikit (Qiagen) according to the manufacturer's protocol.

    Techniques: Real-time Polymerase Chain Reaction, Transfection, Isolation

    The interaction of DDX6 with HCV core and viral RNA is dependent on the C-terminal domain of DDX6. (A) Schematic representation of EYFP-DDX6 and related mutants, ΔC (C-terminal deletion mutant lacking 183 amino acids), EQ (mutation in the DEAD-box helicase motif II involving the substitution of Glu-247 by Gln), and empty vector expressing EYFP alone. The ΔC mutation removes the second of two conserved RecA domains, identified by the shaded bars within the DDX6 sequence. (B) HJ3-5 HCV-infected FT3-7 cells were transfected with DNA vector expressing various forms of EYFP-DDX6 or just the EYFP. Cell lysates were prepared 2 days later and subjected to coimmunoprecipitation with anti-GFP (rabbit polyclonal; Clontech) antibody. Coimmunoprecipitation samples were immunoblotted with GFP (top panel) and core-specific (bottom panel) antibodies. Input represents 1/20 of the immunoprecipitation (IP) for anti-GFP blot and 1/80 of the IP for HCV core blot. Total RNA isolated from immunoprecipitates using an RNeasy minikit (Qiagen) was subjected to RT-PCR for detection of genomic HCV RNA (primers targeting the NS3 region) and GAPDH mRNA. (C) Huh-7-191/20 cells were induced to express HCV core protein by removing tetracycline from the medium for 3 days. Cells were then transfected with various DNAs and subjected to coimmunoprecipitation exactly as described for panel B.

    Journal: Journal of Virology

    Article Title: DDX6 (Rck/p54) Is Required for Efficient Hepatitis C Virus Replication but Not for Internal Ribosome Entry Site-Directed Translation ▿

    doi: 10.1128/JVI.00397-10

    Figure Lengend Snippet: The interaction of DDX6 with HCV core and viral RNA is dependent on the C-terminal domain of DDX6. (A) Schematic representation of EYFP-DDX6 and related mutants, ΔC (C-terminal deletion mutant lacking 183 amino acids), EQ (mutation in the DEAD-box helicase motif II involving the substitution of Glu-247 by Gln), and empty vector expressing EYFP alone. The ΔC mutation removes the second of two conserved RecA domains, identified by the shaded bars within the DDX6 sequence. (B) HJ3-5 HCV-infected FT3-7 cells were transfected with DNA vector expressing various forms of EYFP-DDX6 or just the EYFP. Cell lysates were prepared 2 days later and subjected to coimmunoprecipitation with anti-GFP (rabbit polyclonal; Clontech) antibody. Coimmunoprecipitation samples were immunoblotted with GFP (top panel) and core-specific (bottom panel) antibodies. Input represents 1/20 of the immunoprecipitation (IP) for anti-GFP blot and 1/80 of the IP for HCV core blot. Total RNA isolated from immunoprecipitates using an RNeasy minikit (Qiagen) was subjected to RT-PCR for detection of genomic HCV RNA (primers targeting the NS3 region) and GAPDH mRNA. (C) Huh-7-191/20 cells were induced to express HCV core protein by removing tetracycline from the medium for 3 days. Cells were then transfected with various DNAs and subjected to coimmunoprecipitation exactly as described for panel B.

    Article Snippet: Total RNA was isolated from cells by using the RNeasy minikit (Qiagen) and analyzed using reagents supplied with the NorthernMax kit (Applied Biosystems).

    Techniques: Mutagenesis, Plasmid Preparation, Expressing, Sequencing, Infection, Transfection, Immunoprecipitation, Isolation, Reverse Transcription Polymerase Chain Reaction

    Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the RNeasy plant minikit and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.

    Journal: Journal of Virology

    Article Title: Natural 2?,5?-Phosphodiester Bonds Found at the Ligation Sites of Peach Latent Mosaic Viroid

    doi: 10.1128/JVI.75.1.19-25.2001

    Figure Lengend Snippet: Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the RNeasy plant minikit and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.

    Article Snippet: The first procedure used an RNeasy plant minikit (Qiagen) to prepare RNA from 50 to 100 mg of tissue as specified by the manufacturer.

    Techniques: Polyacrylamide Gel Electrophoresis, Incubation