rneasy minikint  (Qiagen)


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    Structured Review

    Qiagen rneasy minikint
    Rneasy Minikint, supplied by Qiagen, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy minikint/product/Qiagen
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rneasy minikint - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Marker:

    Article Title: Quantification of Changes in Morphology, Mechanotransduction, and Gene Expression in Bovine Articular Chondrocytes in Response to 2-Dimensional Culture Indicates the Existence of a Novel Phenotype
    Article Snippet: Expression Profiling of Expanded Chondrocytes The change in expression of key marker genes was studied by reverse-transcriptase polymerase chain reaction (RT-PCR) at the end of each passage. .. There was 1 µg of RNA sample isolated using RNEasy minikint (Qiagen, Hilden, Germany) and reverse transcribed using the ImPromII Reverse Transcription (RT) System (Promega, Fitchburg, WI) per the manufacturers’ instructions.

    Isolation:

    Article Title: Quantification of Changes in Morphology, Mechanotransduction, and Gene Expression in Bovine Articular Chondrocytes in Response to 2-Dimensional Culture Indicates the Existence of a Novel Phenotype
    Article Snippet: .. There was 1 µg of RNA sample isolated using RNEasy minikint (Qiagen, Hilden, Germany) and reverse transcribed using the ImPromII Reverse Transcription (RT) System (Promega, Fitchburg, WI) per the manufacturers’ instructions. .. Published sequences were used or novel sequences designed using the Primer3 online tool and tested for specificity by running an online alignment search using Basic Local Alignment Search Tool (BLAST) on the National Center for Biotechnology Information (NCBI) website ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Quantification of Changes in Morphology, Mechanotransduction, and Gene Expression in Bovine Articular Chondrocytes in Response to 2-Dimensional Culture Indicates the Existence of a Novel Phenotype
    Article Snippet: Expression Profiling of Expanded Chondrocytes The change in expression of key marker genes was studied by reverse-transcriptase polymerase chain reaction (RT-PCR) at the end of each passage. .. There was 1 µg of RNA sample isolated using RNEasy minikint (Qiagen, Hilden, Germany) and reverse transcribed using the ImPromII Reverse Transcription (RT) System (Promega, Fitchburg, WI) per the manufacturers’ instructions.

    Expressing:

    Article Title: Quantification of Changes in Morphology, Mechanotransduction, and Gene Expression in Bovine Articular Chondrocytes in Response to 2-Dimensional Culture Indicates the Existence of a Novel Phenotype
    Article Snippet: Paragraph title: Expression Profiling of Expanded Chondrocytes ... There was 1 µg of RNA sample isolated using RNEasy minikint (Qiagen, Hilden, Germany) and reverse transcribed using the ImPromII Reverse Transcription (RT) System (Promega, Fitchburg, WI) per the manufacturers’ instructions.

    Polymerase Chain Reaction:

    Article Title: Quantification of Changes in Morphology, Mechanotransduction, and Gene Expression in Bovine Articular Chondrocytes in Response to 2-Dimensional Culture Indicates the Existence of a Novel Phenotype
    Article Snippet: Expression Profiling of Expanded Chondrocytes The change in expression of key marker genes was studied by reverse-transcriptase polymerase chain reaction (RT-PCR) at the end of each passage. .. There was 1 µg of RNA sample isolated using RNEasy minikint (Qiagen, Hilden, Germany) and reverse transcribed using the ImPromII Reverse Transcription (RT) System (Promega, Fitchburg, WI) per the manufacturers’ instructions.

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  • 99
    Qiagen rna extraction kit
    si <t>RNA</t> knockdown of TRPM 7 channel abrogates Ca 2+ influx and I spont in <t>K562</t> cells. (A) detection of TRPM 7 transcripts (398 bp) by conventional RT ‐ PCR . Ten μ mol/L doxycycline ( DXC ) almost completely eliminated TRPM 7 mRNA expression. (B) immunoblots of K562 protein extracts by TRPM 7 antibody. Doxycycline concentration dependently (0.1–10 μ mol/L) reduced TRPM 7 protein expression. The results shown in A and B are representative of three independent experiments. (C) fura‐2 Ca 2+ fluorescence imaging from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing tet ‐inducible TRPM 7‐specific si RNA (si TRPM 7). n = 36 and 26, respectively. (D) density of I spont recorded by conventional whole‐cell recording from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing si TRPM 7. To facilitate the induction of TRPM 7 currents, ATP and Mg 2+ were absent in the patch pipette. The numbers of cells tested are shown in parentheses.
    Rna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3099 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna extraction kit/product/Qiagen
    Average 99 stars, based on 3099 article reviews
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    85
    Qiagen mouse perihematomal tissue
    Molecular networks regulated by ICH in <t>perihematomal</t> tissue. Each quadrant shows a network of interacting genes and gene products whose expression is differentially regulated in response to ICH. Genes colored red are upregulated; genes colored green are
    Mouse Perihematomal Tissue, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse perihematomal tissue/product/Qiagen
    Average 85 stars, based on 1 article reviews
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    Image Search Results


    si RNA knockdown of TRPM 7 channel abrogates Ca 2+ influx and I spont in K562 cells. (A) detection of TRPM 7 transcripts (398 bp) by conventional RT ‐ PCR . Ten μ mol/L doxycycline ( DXC ) almost completely eliminated TRPM 7 mRNA expression. (B) immunoblots of K562 protein extracts by TRPM 7 antibody. Doxycycline concentration dependently (0.1–10 μ mol/L) reduced TRPM 7 protein expression. The results shown in A and B are representative of three independent experiments. (C) fura‐2 Ca 2+ fluorescence imaging from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing tet ‐inducible TRPM 7‐specific si RNA (si TRPM 7). n = 36 and 26, respectively. (D) density of I spont recorded by conventional whole‐cell recording from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing si TRPM 7. To facilitate the induction of TRPM 7 currents, ATP and Mg 2+ were absent in the patch pipette. The numbers of cells tested are shown in parentheses.

    Journal: Physiological Reports

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562

    doi: 10.14814/phy2.13796

    Figure Lengend Snippet: si RNA knockdown of TRPM 7 channel abrogates Ca 2+ influx and I spont in K562 cells. (A) detection of TRPM 7 transcripts (398 bp) by conventional RT ‐ PCR . Ten μ mol/L doxycycline ( DXC ) almost completely eliminated TRPM 7 mRNA expression. (B) immunoblots of K562 protein extracts by TRPM 7 antibody. Doxycycline concentration dependently (0.1–10 μ mol/L) reduced TRPM 7 protein expression. The results shown in A and B are representative of three independent experiments. (C) fura‐2 Ca 2+ fluorescence imaging from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing tet ‐inducible TRPM 7‐specific si RNA (si TRPM 7). n = 36 and 26, respectively. (D) density of I spont recorded by conventional whole‐cell recording from untreated ([ DXC (−)] and 10 μ mol/L doxycycline‐treated [ DXC (+)] K562 cells stably expressing si TRPM 7. To facilitate the induction of TRPM 7 currents, ATP and Mg 2+ were absent in the patch pipette. The numbers of cells tested are shown in parentheses.

    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Concentration Assay, Fluorescence, Imaging, Stable Transfection, Transferring

    K562 cell growth is dependent on Ca 2+ influx mediated by TRPM 7 channel. (A) Dependency of K562 cell growth on extracellular Ca 2+ concentration evaluated at 72 h after culture. Open and filled circles indicate K562 cells untreated [ DXC (‐)] or treated with 10 μ mol/L doxycycline [ DXC (+)], respectively. (B) time courses of K562 cell growth under various conditions, i.e. DXC (−) (no doxycycline and normal Ca 2+ in RPMI medium), DXC (−)0Ca 2+ (no doxycycline and Ca 2+ free in the medium), DXC (+) (10 μ mol/L doxycycline and normal Ca 2+ in the medium) and DXC (+)0Ca 2+ (10 μ mol/L doxycycline and Ca 2+ free in the medium). In both types of experiments (A and B), K562 cells stably expressing tet ‐inducible TRPM 7‐si RNA (si TRPM 7) were used. n = 5 for each condition. * P

    Journal: Physiological Reports

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562

    doi: 10.14814/phy2.13796

    Figure Lengend Snippet: K562 cell growth is dependent on Ca 2+ influx mediated by TRPM 7 channel. (A) Dependency of K562 cell growth on extracellular Ca 2+ concentration evaluated at 72 h after culture. Open and filled circles indicate K562 cells untreated [ DXC (‐)] or treated with 10 μ mol/L doxycycline [ DXC (+)], respectively. (B) time courses of K562 cell growth under various conditions, i.e. DXC (−) (no doxycycline and normal Ca 2+ in RPMI medium), DXC (−)0Ca 2+ (no doxycycline and Ca 2+ free in the medium), DXC (+) (10 μ mol/L doxycycline and normal Ca 2+ in the medium) and DXC (+)0Ca 2+ (10 μ mol/L doxycycline and Ca 2+ free in the medium). In both types of experiments (A and B), K562 cells stably expressing tet ‐inducible TRPM 7‐si RNA (si TRPM 7) were used. n = 5 for each condition. * P

    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA.

    Techniques: Concentration Assay, Stable Transfection, Expressing

    Erythroid differentiation of K562 cell by hemin is regulated by Ca 2+ influx mediated by TRPM 7. (A) representative RT ‐ PCR experiments showing hemin‐induced γ ‐globin synthesis in K562 cells untreated (control) and treated with 10 μ mol/L FTY 720 (+ FTY ), 1 mmol/L EGTA (+ EGTA ) or 10 μ mol/L doxycycline (+dxc). In all conditions, K562 cells stably expressing tet ‐inducible TRPM 7‐si RNA (si TRPM 7) were used. The expression of γ ‐globin mRNA was greatly decreased by these procedues, while that of GAPDH stayed almost constant. (B and C) statistical evaluation of pooled data from experiments as shown in A: 10 μ mol/L FTY 720 and 1 mmol/L EGTA (B): si TRPM 7 (C). (D and E) effects of 10 μ mol/L FTY 720 ( FTY ), 1 mmol/L EGTA ( EGTA ) or 10 μ mol/L doxycycline (+dxc) on hemoglobin synthesis at rest or 3 days after hemin treatment in K562‐cells stably expressing si TRPM 7. n = 5 for each condition. * P

    Journal: Physiological Reports

    Article Title: TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562. TRPM7‐mediated spontaneous Ca2+ entry regulates the proliferation and differentiation of human leukemia cell line K562

    doi: 10.14814/phy2.13796

    Figure Lengend Snippet: Erythroid differentiation of K562 cell by hemin is regulated by Ca 2+ influx mediated by TRPM 7. (A) representative RT ‐ PCR experiments showing hemin‐induced γ ‐globin synthesis in K562 cells untreated (control) and treated with 10 μ mol/L FTY 720 (+ FTY ), 1 mmol/L EGTA (+ EGTA ) or 10 μ mol/L doxycycline (+dxc). In all conditions, K562 cells stably expressing tet ‐inducible TRPM 7‐si RNA (si TRPM 7) were used. The expression of γ ‐globin mRNA was greatly decreased by these procedues, while that of GAPDH stayed almost constant. (B and C) statistical evaluation of pooled data from experiments as shown in A: 10 μ mol/L FTY 720 and 1 mmol/L EGTA (B): si TRPM 7 (C). (D and E) effects of 10 μ mol/L FTY 720 ( FTY ), 1 mmol/L EGTA ( EGTA ) or 10 μ mol/L doxycycline (+dxc) on hemoglobin synthesis at rest or 3 days after hemin treatment in K562‐cells stably expressing si TRPM 7. n = 5 for each condition. * P

    Article Snippet: Reverse transcription polymerase reaction (RT‐PCR) and real‐time PCR Total RNA (about 1 μ g) was extracted from about a million of K562 cells with the total RNA extraction kit (RNeasy Minikit, Qiagen) and reverse transcribed (in a 10 μ L scale) by SuperScriptII (Thermo Fisher Scientific) with a randomized or an oligo‐dT primer to obtain the first strand DNA.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Stable Transfection, Expressing

    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.

    Journal: Journal of Virology

    Article Title: Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game

    doi: 10.1128/JVI.01464-15

    Figure Lengend Snippet: Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.

    Article Snippet: Forty-eight hours after siRNA transfection, total RNA was isolated from mock-infected and transfected HEp-2C cells and Vero cells using an RNeasy minikit (Qiagen) according to the manufacturer's protocol.

    Techniques: Real-time Polymerase Chain Reaction, Transfection, Isolation

    Hbo1 mutant alleles and expression patterns. (A) Schematic of wild-type and Hbo1 mutant alleles, with numbered black boxes representing exons. (B) Three-way PCR used to genotype Hbo1 + (wild-type), Hbo1 lox (floxed), and Hbo1 − (null) alleles, shown here in E8.5 embryos. (C to H) Radioactive RNA in situ hybridization depicting strong and ubiquitous Hbo1 expression at E8.5 and E9.5. Note the particularly high levels of expression in the chorionic plate (CP) at E8.5 and E9.5 as well as in the foregut (FG) and hindgut (HG) regions at E9.5. No Hbo1 mRNA was detectable in Hbo1 −/− embryos (G and H). SP, spongiotrophoblast; AL, allantois; H E, hematoxylin and eosin. (I and J) HBO1 immunohistochemistry shows strong nuclear localization of HBO1 protein in wild-type controls (arrowheads, I), while no HBO1 protein is present in Hbo1 −/− embryos (J). SM, somites. (K) Northern blot showing Hbo1 mRNA expression in adult and embryonic tissues. Bar, 75 μm (C and D), 350 μm (E and F), 212 μm (G and H), or 162 μm (I and J).

    Journal: Molecular and Cellular Biology

    Article Title: HBO1 Is Required for H3K14 Acetylation and Normal Transcriptional Activity during Embryonic Development ▿

    doi: 10.1128/MCB.00159-10

    Figure Lengend Snippet: Hbo1 mutant alleles and expression patterns. (A) Schematic of wild-type and Hbo1 mutant alleles, with numbered black boxes representing exons. (B) Three-way PCR used to genotype Hbo1 + (wild-type), Hbo1 lox (floxed), and Hbo1 − (null) alleles, shown here in E8.5 embryos. (C to H) Radioactive RNA in situ hybridization depicting strong and ubiquitous Hbo1 expression at E8.5 and E9.5. Note the particularly high levels of expression in the chorionic plate (CP) at E8.5 and E9.5 as well as in the foregut (FG) and hindgut (HG) regions at E9.5. No Hbo1 mRNA was detectable in Hbo1 −/− embryos (G and H). SP, spongiotrophoblast; AL, allantois; H E, hematoxylin and eosin. (I and J) HBO1 immunohistochemistry shows strong nuclear localization of HBO1 protein in wild-type controls (arrowheads, I), while no HBO1 protein is present in Hbo1 −/− embryos (J). SM, somites. (K) Northern blot showing Hbo1 mRNA expression in adult and embryonic tissues. Bar, 75 μm (C and D), 350 μm (E and F), 212 μm (G and H), or 162 μm (I and J).

    Article Snippet: For reverse transcriptase quantitative PCR (RT-qPCR) analysis for gene expression, RNA was purified using RNA extraction columns (RNeasy minikit; Qiagen) with an on-column DNase digest.

    Techniques: Mutagenesis, Expressing, Polymerase Chain Reaction, RNA In Situ Hybridization, Immunohistochemistry, Northern Blot

    Molecular networks regulated by ICH in perihematomal tissue. Each quadrant shows a network of interacting genes and gene products whose expression is differentially regulated in response to ICH. Genes colored red are upregulated; genes colored green are

    Journal:

    Article Title: Genomic profiles of damage and protection in human intracerebral hemorrhage

    doi: 10.1038/jcbfm.2008.77

    Figure Lengend Snippet: Molecular networks regulated by ICH in perihematomal tissue. Each quadrant shows a network of interacting genes and gene products whose expression is differentially regulated in response to ICH. Genes colored red are upregulated; genes colored green are

    Article Snippet: Total RNA was isolated from mouse perihematomal tissue (RNeasy minikit, Qiagen, Valencia, CA, USA) and assayed for integrity (Nanochip, Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Expressing

    Gene Expression Profile of Human Perihematomal Tissue

    Journal:

    Article Title: Genomic profiles of damage and protection in human intracerebral hemorrhage

    doi: 10.1038/jcbfm.2008.77

    Figure Lengend Snippet: Gene Expression Profile of Human Perihematomal Tissue

    Article Snippet: Total RNA was isolated from mouse perihematomal tissue (RNeasy minikit, Qiagen, Valencia, CA, USA) and assayed for integrity (Nanochip, Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Expressing

    Mouse ICH model and qRT-PCT testing of differentially regulated genes in human perihematomal tissue. ( A ) T1-weighted MRI image of human ICH case, HS6 (). ( B ) Nissl photomicrograph of mouse ICH model. Boxes in mouse ICH model indicate regions shown

    Journal:

    Article Title: Genomic profiles of damage and protection in human intracerebral hemorrhage

    doi: 10.1038/jcbfm.2008.77

    Figure Lengend Snippet: Mouse ICH model and qRT-PCT testing of differentially regulated genes in human perihematomal tissue. ( A ) T1-weighted MRI image of human ICH case, HS6 (). ( B ) Nissl photomicrograph of mouse ICH model. Boxes in mouse ICH model indicate regions shown

    Article Snippet: Total RNA was isolated from mouse perihematomal tissue (RNeasy minikit, Qiagen, Valencia, CA, USA) and assayed for integrity (Nanochip, Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Magnetic Resonance Imaging

    Cellular pattern of annexins and inflammatory cells in perihematomal tissue. Annexin A2 immunoreactivity in the cortex, white matter, and striatum adjacent to ICH ( A ) and in the contralateral hemisphere ( B ). The area shown is indicated in the two boxes

    Journal:

    Article Title: Genomic profiles of damage and protection in human intracerebral hemorrhage

    doi: 10.1038/jcbfm.2008.77

    Figure Lengend Snippet: Cellular pattern of annexins and inflammatory cells in perihematomal tissue. Annexin A2 immunoreactivity in the cortex, white matter, and striatum adjacent to ICH ( A ) and in the contralateral hemisphere ( B ). The area shown is indicated in the two boxes

    Article Snippet: Total RNA was isolated from mouse perihematomal tissue (RNeasy minikit, Qiagen, Valencia, CA, USA) and assayed for integrity (Nanochip, Agilent Technologies, Santa Clara, CA, USA).

    Techniques:

    Cellular Expression Pattern of Aquaporin 9, CCR1, and IL1R1 expression in perihematomal tissue. ( A ) Aquaporin 9 (red) and GFAP (green) immunostaining in periinfarct tissue. Colocalized staining is seen as yellow (asterisks). ( B ) GFAP (green), IL1R1 (red),

    Journal:

    Article Title: Genomic profiles of damage and protection in human intracerebral hemorrhage

    doi: 10.1038/jcbfm.2008.77

    Figure Lengend Snippet: Cellular Expression Pattern of Aquaporin 9, CCR1, and IL1R1 expression in perihematomal tissue. ( A ) Aquaporin 9 (red) and GFAP (green) immunostaining in periinfarct tissue. Colocalized staining is seen as yellow (asterisks). ( B ) GFAP (green), IL1R1 (red),

    Article Snippet: Total RNA was isolated from mouse perihematomal tissue (RNeasy minikit, Qiagen, Valencia, CA, USA) and assayed for integrity (Nanochip, Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Expressing, Immunostaining, Staining

    Microarray analysis of human perihematomal brain samples. ( A ) Standard deviation of array signals across control and experimental cases after RMA normalization. Lines in each column indicate mean and boxes indicated 2 standard deviations. Red boxes are

    Journal:

    Article Title: Genomic profiles of damage and protection in human intracerebral hemorrhage

    doi: 10.1038/jcbfm.2008.77

    Figure Lengend Snippet: Microarray analysis of human perihematomal brain samples. ( A ) Standard deviation of array signals across control and experimental cases after RMA normalization. Lines in each column indicate mean and boxes indicated 2 standard deviations. Red boxes are

    Article Snippet: Total RNA was isolated from mouse perihematomal tissue (RNeasy minikit, Qiagen, Valencia, CA, USA) and assayed for integrity (Nanochip, Agilent Technologies, Santa Clara, CA, USA).

    Techniques: Microarray, Standard Deviation