rneasy mini kit  (Qiagen)


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    Structured Review

    Qiagen rneasy mini kit
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 8038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy mini kit/product/Qiagen
    Average 99 stars, based on 8038 article reviews
    Price from $9.99 to $1999.99
    rneasy mini kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: RNAseq Analysis Highlights Specific Transcriptome Signatures of Yeast and Mycelial Growth Phases in the Dutch Elm Disease Fungus Ophiostoma novo-ulmi
    Article Snippet: RNA extraction, cDNA library production, and RNA sequencing Samples were harvested by centrifugation (5 min, 4700 g, 4°) for liquid cultures or by collecting mycelium directly on the membrane for solid cultures. .. Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, yeast protocol) and quality control was assured using a spectrophotometer (NanoDrop ND-1000, Thermo Scientific) and a bioanalyzer (BioAnalyzer RNA 6000 Nano Kit, Agilent Technologies).

    Amplification:

    Article Title: Differential regulation of miRNA and mRNA expression in the myocardium of Nrf2 knockout mice
    Article Snippet: Next generation mRNA sequencing Cardiac RNA was isolated from 6 to 8 month male WT and Nrf2−/− (n = 3–4/group) using the RNeasy Mini Kit (Qiagen, Cat. 74,106) according to the manufacturer’s instructions. .. The cBot was used to apply 18pM of the sequencing library to a TruSeq v3 flowcell (Illumina) and the TruSeq SR Cluster Kit (Illumina, Cat. GD-401-3001) was used for clonal amplification.

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Microarray Expression Analysis Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Synthesized:

    Article Title: RNAseq Analysis Highlights Specific Transcriptome Signatures of Yeast and Mycelial Growth Phases in the Dutch Elm Disease Fungus Ophiostoma novo-ulmi
    Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, yeast protocol) and quality control was assured using a spectrophotometer (NanoDrop ND-1000, Thermo Scientific) and a bioanalyzer (BioAnalyzer RNA 6000 Nano Kit, Agilent Technologies). .. Complementary DNA (cDNA) libraries were synthesized using the TruSeq RNA Sample Preparation Kit v2 (Illumina) with 1 μg of starting material per sample at the Plateforme d’Analyses Génomiques (IBIS/Université Laval).

    Article Title: Clinical relevance of the transcriptional signature regulated by CDC42 in colorectal cancer
    Article Snippet: RNA extraction from cell lines and tissues and qPCR Total RNA was prepared from the cell lines using the RNeasy Mini kit (Qiagen, Hilden, Germany) and prepared from xenograft and patient tissues using Trizol® Reagent (Invitrogen) and cleaned up with RNeasy Mini kit (Qiagen) by following the manufacturer's instructions. .. First strand cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Archive Kit (Applied Biosystems) at 37°C for 2 h. The expression of CACNA2D2 and CDC42 genes was quantified by real-time PCR.

    Article Title: Functional Annotation of the Ophiostoma novo-ulmi Genome: Insights into the Phytopathogenicity of the Fungal Agent of Dutch Elm Disease
    Article Snippet: .. RNA-seq Analysis Using techniques similar to those listed above, yeast- (liquid) and mycelial-form (solid) H327 cultures grown in/on complete medium ( ) were harvested after 5 days growth, RNAs were extracted using the RNeasy Mini Kit (QIAGEN; yeast protocol) and nucleic acids were quality controlled using the NanoDrop and BioAnalyzer RNA 6000 Nano Kit (Agilent Technologies). mRNA-focused cDNA libraries were synthesized using the TruSeq RNA Sample Preparation Kit v2 (Illumina) using 1 µg of starting material at the IBIS/Université Laval Plate-forme d’Analyses Génomiques. .. Libraries were sequenced on one lane of an Illumina HiSeq 2500 (v1.9 single-end 100 bp) at the McGill University and Génome Québec Innovation Centre.

    SYBR Green Assay:

    Article Title: The effect of mutant SOD1 dismutase activity on non-cell autonomous degeneration in familial amyotrophic lateral sclerosis
    Article Snippet: Approximately 1200 MNs from the anterior horn of each mouse spinal cord were collected into 75 μl of RLT buffer (Qiagen RNeasy Mini Kit, QIAGEN, Valencia, CA), and RNA was isolated using an RNeasy Mini Kit (QIAGEN). .. The RNA from the MNs was treated with DNase I (Invitrogen, Carlsbad, CA) to eliminate genomic DNA contamination followed by first-strand cDNA synthesis and qPCR using a SuperScript III two-step qPCR kit with Sybr green (Invitrogen). qPCR was performed on an iCycler Thermal Cycler machine (Biorad, Hercules, CA) using the following protocol: 1 cycle 50°C for 2 min, 1 cycle 95°C for 2 min; 40 cycles 95°C for 15s, 60°C for 30s.

    Microarray:

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Paragraph title: Microarray Expression Analysis ... Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions.

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: .. Microarray Expression Analysis Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Expressing:

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Paragraph title: Microarray Expression Analysis ... Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions.

    Article Title: The effect of mutant SOD1 dismutase activity on non-cell autonomous degeneration in familial amyotrophic lateral sclerosis
    Article Snippet: Paragraph title: Studies of human SOD1 expression ... Approximately 1200 MNs from the anterior horn of each mouse spinal cord were collected into 75 μl of RLT buffer (Qiagen RNeasy Mini Kit, QIAGEN, Valencia, CA), and RNA was isolated using an RNeasy Mini Kit (QIAGEN).

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: .. Microarray Expression Analysis Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Article Title: Clinical relevance of the transcriptional signature regulated by CDC42 in colorectal cancer
    Article Snippet: RNA extraction from cell lines and tissues and qPCR Total RNA was prepared from the cell lines using the RNeasy Mini kit (Qiagen, Hilden, Germany) and prepared from xenograft and patient tissues using Trizol® Reagent (Invitrogen) and cleaned up with RNeasy Mini kit (Qiagen) by following the manufacturer's instructions. .. First strand cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Archive Kit (Applied Biosystems) at 37°C for 2 h. The expression of CACNA2D2 and CDC42 genes was quantified by real-time PCR.

    Article Title: Functional Annotation of the Ophiostoma novo-ulmi Genome: Insights into the Phytopathogenicity of the Fungal Agent of Dutch Elm Disease
    Article Snippet: RNA-seq Analysis Using techniques similar to those listed above, yeast- (liquid) and mycelial-form (solid) H327 cultures grown in/on complete medium ( ) were harvested after 5 days growth, RNAs were extracted using the RNeasy Mini Kit (QIAGEN; yeast protocol) and nucleic acids were quality controlled using the NanoDrop and BioAnalyzer RNA 6000 Nano Kit (Agilent Technologies). mRNA-focused cDNA libraries were synthesized using the TruSeq RNA Sample Preparation Kit v2 (Illumina) using 1 µg of starting material at the IBIS/Université Laval Plate-forme d’Analyses Génomiques. .. The two samples (yeast + mycelial) utilized herein for gene correction were part of 12 bar-coded samples run on the same above lane that will be the subject of a forthcoming study on H327 dimorphism expression profiling (Nigg M and Bernier L, unpublished data).

    Real-time Polymerase Chain Reaction:

    Article Title: Study of Carbon Nano-Tubes Effects on the Chondrogenesis of Human Adipose Derived Stem Cells in Alginate Scaffold
    Article Snippet: Paragraph title: Real-time polymerase chain reaction (PCR) ... The resultant solution was centrifuged for 10 min at 1200 rpm and the derived cell pellet was used for the ribonucleic acid (RNA) extraction with RNeasy mini kit (Qiagen, Cat. No.

    Article Title: The effect of mutant SOD1 dismutase activity on non-cell autonomous degeneration in familial amyotrophic lateral sclerosis
    Article Snippet: In order to determine the amount of knockdown of human SOD1 in MNs following Cre excision, we performed laser capture microdissection followed by real-time reverse transcriptase-polymerase chain reaction (qPCR). .. Approximately 1200 MNs from the anterior horn of each mouse spinal cord were collected into 75 μl of RLT buffer (Qiagen RNeasy Mini Kit, QIAGEN, Valencia, CA), and RNA was isolated using an RNeasy Mini Kit (QIAGEN).

    Article Title: Clinical relevance of the transcriptional signature regulated by CDC42 in colorectal cancer
    Article Snippet: .. RNA extraction from cell lines and tissues and qPCR Total RNA was prepared from the cell lines using the RNeasy Mini kit (Qiagen, Hilden, Germany) and prepared from xenograft and patient tissues using Trizol® Reagent (Invitrogen) and cleaned up with RNeasy Mini kit (Qiagen) by following the manufacturer's instructions. .. Total RNA from each sample was quantified by the Nanodrop ND 1000 and RNA integrity was assessed by standard electrophoresis on agarose gel.

    Derivative Assay:

    Article Title: Study of Carbon Nano-Tubes Effects on the Chondrogenesis of Human Adipose Derived Stem Cells in Alginate Scaffold
    Article Snippet: .. The resultant solution was centrifuged for 10 min at 1200 rpm and the derived cell pellet was used for the ribonucleic acid (RNA) extraction with RNeasy mini kit (Qiagen, Cat. No. .. [ ] For lysing cells, first, a 990 μl Trizol (Invitrogen) and 10 μl of 2-mercaptoethanol (Sigma) solution was mixed and kept in room temperature (RT) for 5 min. Next, 200 μl chloroform was added and shacked firmly for 15 s and was kept in RT for 2-3 min and centrifuged for 15 min at 4° at 12,000 g. The supernatant aqueous phase was transferred to 1.5 ml microtube and the same volume of ethanol 70% was added and mixed.

    Infection:

    Article Title: Mycobacterium tuberculosis–induced IFN-β production requires cytosolic DNA and RNA sensing pathways
    Article Snippet: .. Total cellular RNA was isolated from infected cells using Qiagen RNeasy Mini Kit (cat. 74136; Qiagen), and cytosolic RNA from cytosolic fraction by ethanol precipitation. .. For cytosolic RNA, cells were washed with precold PBS three times and then resuspended in lysis buffer (20 mM Hepes [pH 7.4], 250 mM sucrose, 10 mM KCl, 2 mM MgCl2 , and 1 mM EDTA) plus RNase inhibitor (M0314; NEB) and disrupted with a syringe and 27 G needle by 25 strikes.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: GINS, a central nexus in the archaeal DNA replication fork
    Article Snippet: RNA was extracted using the RNeasy Mini Kit (Qiagen, Crawley, UK) and treated with 7.5 U RNase-free DNaseI (Roche, Basel, Switzerland) for 1 h at 37°C in a 50 μl reaction containing 0.25 × NEBuffer 3 (New England Biolabs, Ipswich, MA, USA), and cleaned up with the RNeasy Mini Kit (Qiagen). .. A 10 μg portion of RNA was used with the OneStep RT–PCR Kit (Qiagen) under the following PCR conditions: 45 s denaturation, 45 s annealing (60°C), 29 cycles.

    Sequencing:

    Article Title: Differential regulation of miRNA and mRNA expression in the myocardium of Nrf2 knockout mice
    Article Snippet: .. Next generation mRNA sequencing Cardiac RNA was isolated from 6 to 8 month male WT and Nrf2−/− (n = 3–4/group) using the RNeasy Mini Kit (Qiagen, Cat. 74,106) according to the manufacturer’s instructions. .. After confirmation of sample purity, intact poly(A) transcripts were purified from total RNA using oligo(dT) magnetic beads and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS-122-2102).

    Article Title: The effect of mutant SOD1 dismutase activity on non-cell autonomous degeneration in familial amyotrophic lateral sclerosis
    Article Snippet: Approximately 1200 MNs from the anterior horn of each mouse spinal cord were collected into 75 μl of RLT buffer (Qiagen RNeasy Mini Kit, QIAGEN, Valencia, CA), and RNA was isolated using an RNeasy Mini Kit (QIAGEN). .. Details were previously published regarding the sequence of the primers and probes for SOD1 ( ) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ( ).

    Article Title: Clinical relevance of the transcriptional signature regulated by CDC42 in colorectal cancer
    Article Snippet: RNA extraction from cell lines and tissues and qPCR Total RNA was prepared from the cell lines using the RNeasy Mini kit (Qiagen, Hilden, Germany) and prepared from xenograft and patient tissues using Trizol® Reagent (Invitrogen) and cleaned up with RNeasy Mini kit (Qiagen) by following the manufacturer's instructions. .. Each cDNA sample was analyzed in triplicate using the ABI PRISM 770 Sequence Detector (Applied Biosystems).

    Article Title: Functional Annotation of the Ophiostoma novo-ulmi Genome: Insights into the Phytopathogenicity of the Fungal Agent of Dutch Elm Disease
    Article Snippet: RNA-seq Analysis Using techniques similar to those listed above, yeast- (liquid) and mycelial-form (solid) H327 cultures grown in/on complete medium ( ) were harvested after 5 days growth, RNAs were extracted using the RNeasy Mini Kit (QIAGEN; yeast protocol) and nucleic acids were quality controlled using the NanoDrop and BioAnalyzer RNA 6000 Nano Kit (Agilent Technologies). mRNA-focused cDNA libraries were synthesized using the TruSeq RNA Sample Preparation Kit v2 (Illumina) using 1 µg of starting material at the IBIS/Université Laval Plate-forme d’Analyses Génomiques. .. The raw RNA-seq data were submitted to the NCBI Sequence Read Archive under accession SRP047075.

    RNA Sequencing Assay:

    Article Title: RNAseq Analysis Highlights Specific Transcriptome Signatures of Yeast and Mycelial Growth Phases in the Dutch Elm Disease Fungus Ophiostoma novo-ulmi
    Article Snippet: Paragraph title: RNA extraction, cDNA library production, and RNA sequencing ... Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, yeast protocol) and quality control was assured using a spectrophotometer (NanoDrop ND-1000, Thermo Scientific) and a bioanalyzer (BioAnalyzer RNA 6000 Nano Kit, Agilent Technologies).

    Article Title: Functional Annotation of the Ophiostoma novo-ulmi Genome: Insights into the Phytopathogenicity of the Fungal Agent of Dutch Elm Disease
    Article Snippet: .. RNA-seq Analysis Using techniques similar to those listed above, yeast- (liquid) and mycelial-form (solid) H327 cultures grown in/on complete medium ( ) were harvested after 5 days growth, RNAs were extracted using the RNeasy Mini Kit (QIAGEN; yeast protocol) and nucleic acids were quality controlled using the NanoDrop and BioAnalyzer RNA 6000 Nano Kit (Agilent Technologies). mRNA-focused cDNA libraries were synthesized using the TruSeq RNA Sample Preparation Kit v2 (Illumina) using 1 µg of starting material at the IBIS/Université Laval Plate-forme d’Analyses Génomiques. .. Libraries were sequenced on one lane of an Illumina HiSeq 2500 (v1.9 single-end 100 bp) at the McGill University and Génome Québec Innovation Centre.

    Magnetic Beads:

    Article Title: Differential regulation of miRNA and mRNA expression in the myocardium of Nrf2 knockout mice
    Article Snippet: Next generation mRNA sequencing Cardiac RNA was isolated from 6 to 8 month male WT and Nrf2−/− (n = 3–4/group) using the RNeasy Mini Kit (Qiagen, Cat. 74,106) according to the manufacturer’s instructions. .. After confirmation of sample purity, intact poly(A) transcripts were purified from total RNA using oligo(dT) magnetic beads and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS-122-2102).

    Isolation:

    Article Title: The Ameliorative Effects of a Tocotrienol-Rich Fraction on the AGE-RAGE Axis and Hypertension in High-Fat-Diet-Fed Rats with Metabolic Syndrome
    Article Snippet: .. RNA Extraction and cDNA Synthesis Total RNA of the rWAT was isolated with Tri-RNA reagent (Favorgen, Ping-Tung, Taiwan) followed by the Qiagen RNeasy Mini kit (Qiagen, Hilden, Germany) to clean up the RNA sample, while that of the liver was extracted with Qiagen RNeasy Mini kit directly. .. The concentration and purity of the RNA were determined by measuring the absorbance at 260 nm and 280 nm with Infinite® 200 PRO (TECAN, Zürich, Switzerland).

    Article Title: Mycobacterium tuberculosis–induced IFN-β production requires cytosolic DNA and RNA sensing pathways
    Article Snippet: .. Total cellular RNA was isolated from infected cells using Qiagen RNeasy Mini Kit (cat. 74136; Qiagen), and cytosolic RNA from cytosolic fraction by ethanol precipitation. .. For cytosolic RNA, cells were washed with precold PBS three times and then resuspended in lysis buffer (20 mM Hepes [pH 7.4], 250 mM sucrose, 10 mM KCl, 2 mM MgCl2 , and 1 mM EDTA) plus RNase inhibitor (M0314; NEB) and disrupted with a syringe and 27 G needle by 25 strikes.

    Article Title: Increased susceptibility of post-weaning rats on high-fat diet to metabolic syndrome
    Article Snippet: .. RNA extraction and cDNA synthesis Total RNA extraction of the liver was conducted using Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany), whereas that of rWAT was isolated with Tri-RNA reagent (Favorgen, Ping-Tung, Taiwan) and Qiagen RNeasy Mini Kit. .. The concentration and purity of the RNA were determined by measuring the absorbance at 260 nm and 280 nm with Infinite® 200 PRO (TECAN, Zürich, Switzerland).

    Article Title: Differential regulation of miRNA and mRNA expression in the myocardium of Nrf2 knockout mice
    Article Snippet: .. Next generation mRNA sequencing Cardiac RNA was isolated from 6 to 8 month male WT and Nrf2−/− (n = 3–4/group) using the RNeasy Mini Kit (Qiagen, Cat. 74,106) according to the manufacturer’s instructions. .. After confirmation of sample purity, intact poly(A) transcripts were purified from total RNA using oligo(dT) magnetic beads and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS-122-2102).

    Article Title: One millimetre makes the difference: high-resolution analysis of methane-oxidizing bacteria and their specific activity at the oxic-anoxic interface in a flooded paddy soil
    Article Snippet: .. RNA was prepared by digestion of 1 mg total nucleic acid with RQ1 RNase-free DNase (Promega, Madison, WI, USA) and subsequent purification using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to manufacturer's instructions. pmoA transcripts were enriched using the mRNA-only Prokaryotic mRNA Isolation Kit (Epicentre Biotechnologies, Madison, WI, USA) and again purified with the RNeasy Mini Kit (Qiagen). .. cDNA was synthesized and pmoA was amplified using the One-step Access RT-PCR System (Promega) with the forward primer A189f (5′-GGNGACTGGGACTTCTGG-3′) ( ) and the reverse primer mb661r (5′-CCGGMGCAACGTCYTTACC-3′) ( ).

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: .. Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Article Title: The effect of mutant SOD1 dismutase activity on non-cell autonomous degeneration in familial amyotrophic lateral sclerosis
    Article Snippet: .. Approximately 1200 MNs from the anterior horn of each mouse spinal cord were collected into 75 μl of RLT buffer (Qiagen RNeasy Mini Kit, QIAGEN, Valencia, CA), and RNA was isolated using an RNeasy Mini Kit (QIAGEN). .. The RNA from the MNs was treated with DNase I (Invitrogen, Carlsbad, CA) to eliminate genomic DNA contamination followed by first-strand cDNA synthesis and qPCR using a SuperScript III two-step qPCR kit with Sybr green (Invitrogen). qPCR was performed on an iCycler Thermal Cycler machine (Biorad, Hercules, CA) using the following protocol: 1 cycle 50°C for 2 min, 1 cycle 95°C for 2 min; 40 cycles 95°C for 15s, 60°C for 30s.

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: .. Microarray Expression Analysis Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Labeling:

    Article Title: Mycobacterium tuberculosis–induced IFN-β production requires cytosolic DNA and RNA sensing pathways
    Article Snippet: The Raw264.7 cells were infected with labeled M.tb strains at a MOI of 10 for 24 h at 37°C. .. Total cellular RNA was isolated from infected cells using Qiagen RNeasy Mini Kit (cat. 74136; Qiagen), and cytosolic RNA from cytosolic fraction by ethanol precipitation.

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Microarray Expression Analysis Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Purification:

    Article Title: Mycobacterium tuberculosis–induced IFN-β production requires cytosolic DNA and RNA sensing pathways
    Article Snippet: Paragraph title: Purification of EU-labeled M.tb RNAs from infected host cells ... Total cellular RNA was isolated from infected cells using Qiagen RNeasy Mini Kit (cat. 74136; Qiagen), and cytosolic RNA from cytosolic fraction by ethanol precipitation.

    Article Title: Differential regulation of miRNA and mRNA expression in the myocardium of Nrf2 knockout mice
    Article Snippet: Next generation mRNA sequencing Cardiac RNA was isolated from 6 to 8 month male WT and Nrf2−/− (n = 3–4/group) using the RNeasy Mini Kit (Qiagen, Cat. 74,106) according to the manufacturer’s instructions. .. After confirmation of sample purity, intact poly(A) transcripts were purified from total RNA using oligo(dT) magnetic beads and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS-122-2102).

    Article Title: Microarray Expression Analysis of the Main Inflorescence in Brassica napus
    Article Snippet: RNA extraction Using an RNeasy Mini Kit (Cat. 74124, Qiagen, Mississauga, ON) according to the manufacturer's recommendations, the total RNA of inflorescence primordia was extracted in biological triplicate, each consisting of three main inflorescence sections taken from three independent plants. .. The RNA yield and purity were determined spectrophotometrically with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, USA), and the intactness of RNA was verified by electrophoresis on a 1% agarose gel with 1×TBE running buffer at 70 V for 40 min. Purified total RNA was precipitated and re-suspended in DEPC-treated water to a final concentration of about 500 ng/µL.

    Article Title: One millimetre makes the difference: high-resolution analysis of methane-oxidizing bacteria and their specific activity at the oxic-anoxic interface in a flooded paddy soil
    Article Snippet: .. RNA was prepared by digestion of 1 mg total nucleic acid with RQ1 RNase-free DNase (Promega, Madison, WI, USA) and subsequent purification using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to manufacturer's instructions. pmoA transcripts were enriched using the mRNA-only Prokaryotic mRNA Isolation Kit (Epicentre Biotechnologies, Madison, WI, USA) and again purified with the RNeasy Mini Kit (Qiagen). .. cDNA was synthesized and pmoA was amplified using the One-step Access RT-PCR System (Promega) with the forward primer A189f (5′-GGNGACTGGGACTTCTGG-3′) ( ) and the reverse primer mb661r (5′-CCGGMGCAACGTCYTTACC-3′) ( ).

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: .. Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: .. Microarray Expression Analysis Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Polymerase Chain Reaction:

    Article Title: Study of Carbon Nano-Tubes Effects on the Chondrogenesis of Human Adipose Derived Stem Cells in Alginate Scaffold
    Article Snippet: Paragraph title: Real-time polymerase chain reaction (PCR) ... The resultant solution was centrifuged for 10 min at 1200 rpm and the derived cell pellet was used for the ribonucleic acid (RNA) extraction with RNeasy mini kit (Qiagen, Cat. No.

    Article Title: GINS, a central nexus in the archaeal DNA replication fork
    Article Snippet: RNA was extracted using the RNeasy Mini Kit (Qiagen, Crawley, UK) and treated with 7.5 U RNase-free DNaseI (Roche, Basel, Switzerland) for 1 h at 37°C in a 50 μl reaction containing 0.25 × NEBuffer 3 (New England Biolabs, Ipswich, MA, USA), and cleaned up with the RNeasy Mini Kit (Qiagen). .. A 10 μg portion of RNA was used with the OneStep RT–PCR Kit (Qiagen) under the following PCR conditions: 45 s denaturation, 45 s annealing (60°C), 29 cycles.

    Lysis:

    Article Title: Mycobacterium tuberculosis–induced IFN-β production requires cytosolic DNA and RNA sensing pathways
    Article Snippet: Total cellular RNA was isolated from infected cells using Qiagen RNeasy Mini Kit (cat. 74136; Qiagen), and cytosolic RNA from cytosolic fraction by ethanol precipitation. .. For cytosolic RNA, cells were washed with precold PBS three times and then resuspended in lysis buffer (20 mM Hepes [pH 7.4], 250 mM sucrose, 10 mM KCl, 2 mM MgCl2 , and 1 mM EDTA) plus RNase inhibitor (M0314; NEB) and disrupted with a syringe and 27 G needle by 25 strikes.

    cDNA Library Assay:

    Article Title: RNAseq Analysis Highlights Specific Transcriptome Signatures of Yeast and Mycelial Growth Phases in the Dutch Elm Disease Fungus Ophiostoma novo-ulmi
    Article Snippet: Paragraph title: RNA extraction, cDNA library production, and RNA sequencing ... Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, yeast protocol) and quality control was assured using a spectrophotometer (NanoDrop ND-1000, Thermo Scientific) and a bioanalyzer (BioAnalyzer RNA 6000 Nano Kit, Agilent Technologies).

    Agarose Gel Electrophoresis:

    Article Title: The Ameliorative Effects of a Tocotrienol-Rich Fraction on the AGE-RAGE Axis and Hypertension in High-Fat-Diet-Fed Rats with Metabolic Syndrome
    Article Snippet: RNA Extraction and cDNA Synthesis Total RNA of the rWAT was isolated with Tri-RNA reagent (Favorgen, Ping-Tung, Taiwan) followed by the Qiagen RNeasy Mini kit (Qiagen, Hilden, Germany) to clean up the RNA sample, while that of the liver was extracted with Qiagen RNeasy Mini kit directly. .. RNA integrity was examined with agarose gel electrophoresis to check 18 S and 28 S ribosomal RNA.

    Article Title: Increased susceptibility of post-weaning rats on high-fat diet to metabolic syndrome
    Article Snippet: RNA extraction and cDNA synthesis Total RNA extraction of the liver was conducted using Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany), whereas that of rWAT was isolated with Tri-RNA reagent (Favorgen, Ping-Tung, Taiwan) and Qiagen RNeasy Mini Kit. .. RNA integrity was examined with agarose gel electrophoresis to check 18S and 28S ribosomal RNA.

    Article Title: Microarray Expression Analysis of the Main Inflorescence in Brassica napus
    Article Snippet: RNA extraction Using an RNeasy Mini Kit (Cat. 74124, Qiagen, Mississauga, ON) according to the manufacturer's recommendations, the total RNA of inflorescence primordia was extracted in biological triplicate, each consisting of three main inflorescence sections taken from three independent plants. .. The RNA yield and purity were determined spectrophotometrically with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, USA), and the intactness of RNA was verified by electrophoresis on a 1% agarose gel with 1×TBE running buffer at 70 V for 40 min. Purified total RNA was precipitated and re-suspended in DEPC-treated water to a final concentration of about 500 ng/µL.

    Article Title: Clinical relevance of the transcriptional signature regulated by CDC42 in colorectal cancer
    Article Snippet: RNA extraction from cell lines and tissues and qPCR Total RNA was prepared from the cell lines using the RNeasy Mini kit (Qiagen, Hilden, Germany) and prepared from xenograft and patient tissues using Trizol® Reagent (Invitrogen) and cleaned up with RNeasy Mini kit (Qiagen) by following the manufacturer's instructions. .. Total RNA from each sample was quantified by the Nanodrop ND 1000 and RNA integrity was assessed by standard electrophoresis on agarose gel.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Identification of a common reference gene pair for qPCR in human mesenchymal stromal cells from different tissue sources treated with VEGF
    Article Snippet: .. Nucleic acid extraction Total RNA was extracted using Qiagen RNeasy® Mini Kit (Qiagen Hamburg GmbH, Hamburg, Germany, cat.no. .. 1 ml syringe (Omnific‒F 1 ml, B.Braun Melsunger AG, Germany, cat.no.

    Chromatin Immunoprecipitation:

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Microarray Expression Analysis Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. For microarray analysis, total RNA was amplified and transcribed into fluorescent cRNA with Agilent Quick Amp Labeling protocol (version 5.7; Agilent Technologies), and then hybridized to the Agilent SureHyb microarray chip (Agilent Technologies).

    Software:

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. The Agilent Feature Extraction software (v11.0.0.1) was used to analyze the acquired array images and obtain scaled quantitative information.

    Article Title: COMMD7 Regulates NF-κB Signaling Pathway in Hepatocellular Carcinoma Stem-like Cells
    Article Snippet: Microarray Expression Analysis Total RNAs were isolated from cells using the QIAGEN RNeasy Mini Kit (Valencia, CA, USA) and purified using the RNeasy Mini kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions. .. The Agilent Feature Extraction software (v11.0.0.1) was used to analyze the acquired array images and obtain scaled quantitative information.

    Electrophoresis:

    Article Title: Microarray Expression Analysis of the Main Inflorescence in Brassica napus
    Article Snippet: RNA extraction Using an RNeasy Mini Kit (Cat. 74124, Qiagen, Mississauga, ON) according to the manufacturer's recommendations, the total RNA of inflorescence primordia was extracted in biological triplicate, each consisting of three main inflorescence sections taken from three independent plants. .. The RNA yield and purity were determined spectrophotometrically with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, USA), and the intactness of RNA was verified by electrophoresis on a 1% agarose gel with 1×TBE running buffer at 70 V for 40 min. Purified total RNA was precipitated and re-suspended in DEPC-treated water to a final concentration of about 500 ng/µL.

    Article Title: Clinical relevance of the transcriptional signature regulated by CDC42 in colorectal cancer
    Article Snippet: RNA extraction from cell lines and tissues and qPCR Total RNA was prepared from the cell lines using the RNeasy Mini kit (Qiagen, Hilden, Germany) and prepared from xenograft and patient tissues using Trizol® Reagent (Invitrogen) and cleaned up with RNeasy Mini kit (Qiagen) by following the manufacturer's instructions. .. Total RNA from each sample was quantified by the Nanodrop ND 1000 and RNA integrity was assessed by standard electrophoresis on agarose gel.

    RNA Extraction:

    Article Title: Study of Carbon Nano-Tubes Effects on the Chondrogenesis of Human Adipose Derived Stem Cells in Alginate Scaffold
    Article Snippet: .. The resultant solution was centrifuged for 10 min at 1200 rpm and the derived cell pellet was used for the ribonucleic acid (RNA) extraction with RNeasy mini kit (Qiagen, Cat. No. .. [ ] For lysing cells, first, a 990 μl Trizol (Invitrogen) and 10 μl of 2-mercaptoethanol (Sigma) solution was mixed and kept in room temperature (RT) for 5 min. Next, 200 μl chloroform was added and shacked firmly for 15 s and was kept in RT for 2-3 min and centrifuged for 15 min at 4° at 12,000 g. The supernatant aqueous phase was transferred to 1.5 ml microtube and the same volume of ethanol 70% was added and mixed.

    Article Title: The Ameliorative Effects of a Tocotrienol-Rich Fraction on the AGE-RAGE Axis and Hypertension in High-Fat-Diet-Fed Rats with Metabolic Syndrome
    Article Snippet: .. RNA Extraction and cDNA Synthesis Total RNA of the rWAT was isolated with Tri-RNA reagent (Favorgen, Ping-Tung, Taiwan) followed by the Qiagen RNeasy Mini kit (Qiagen, Hilden, Germany) to clean up the RNA sample, while that of the liver was extracted with Qiagen RNeasy Mini kit directly. .. The concentration and purity of the RNA were determined by measuring the absorbance at 260 nm and 280 nm with Infinite® 200 PRO (TECAN, Zürich, Switzerland).

    Article Title: Increased susceptibility of post-weaning rats on high-fat diet to metabolic syndrome
    Article Snippet: .. RNA extraction and cDNA synthesis Total RNA extraction of the liver was conducted using Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany), whereas that of rWAT was isolated with Tri-RNA reagent (Favorgen, Ping-Tung, Taiwan) and Qiagen RNeasy Mini Kit. .. The concentration and purity of the RNA were determined by measuring the absorbance at 260 nm and 280 nm with Infinite® 200 PRO (TECAN, Zürich, Switzerland).

    Article Title: RNAseq Analysis Highlights Specific Transcriptome Signatures of Yeast and Mycelial Growth Phases in the Dutch Elm Disease Fungus Ophiostoma novo-ulmi
    Article Snippet: Paragraph title: RNA extraction, cDNA library production, and RNA sequencing ... Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, yeast protocol) and quality control was assured using a spectrophotometer (NanoDrop ND-1000, Thermo Scientific) and a bioanalyzer (BioAnalyzer RNA 6000 Nano Kit, Agilent Technologies).

    Article Title: Microarray Expression Analysis of the Main Inflorescence in Brassica napus
    Article Snippet: .. RNA extraction Using an RNeasy Mini Kit (Cat. 74124, Qiagen, Mississauga, ON) according to the manufacturer's recommendations, the total RNA of inflorescence primordia was extracted in biological triplicate, each consisting of three main inflorescence sections taken from three independent plants. .. Trace amounts of DNA were removed using DNase I (Cat. 18068-015, Invitrogen, USA).

    Article Title: Clinical relevance of the transcriptional signature regulated by CDC42 in colorectal cancer
    Article Snippet: .. RNA extraction from cell lines and tissues and qPCR Total RNA was prepared from the cell lines using the RNeasy Mini kit (Qiagen, Hilden, Germany) and prepared from xenograft and patient tissues using Trizol® Reagent (Invitrogen) and cleaned up with RNeasy Mini kit (Qiagen) by following the manufacturer's instructions. .. Total RNA from each sample was quantified by the Nanodrop ND 1000 and RNA integrity was assessed by standard electrophoresis on agarose gel.

    Sample Prep:

    Article Title: RNAseq Analysis Highlights Specific Transcriptome Signatures of Yeast and Mycelial Growth Phases in the Dutch Elm Disease Fungus Ophiostoma novo-ulmi
    Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, yeast protocol) and quality control was assured using a spectrophotometer (NanoDrop ND-1000, Thermo Scientific) and a bioanalyzer (BioAnalyzer RNA 6000 Nano Kit, Agilent Technologies). .. Complementary DNA (cDNA) libraries were synthesized using the TruSeq RNA Sample Preparation Kit v2 (Illumina) with 1 μg of starting material per sample at the Plateforme d’Analyses Génomiques (IBIS/Université Laval).

    Article Title: Functional Annotation of the Ophiostoma novo-ulmi Genome: Insights into the Phytopathogenicity of the Fungal Agent of Dutch Elm Disease
    Article Snippet: .. RNA-seq Analysis Using techniques similar to those listed above, yeast- (liquid) and mycelial-form (solid) H327 cultures grown in/on complete medium ( ) were harvested after 5 days growth, RNAs were extracted using the RNeasy Mini Kit (QIAGEN; yeast protocol) and nucleic acids were quality controlled using the NanoDrop and BioAnalyzer RNA 6000 Nano Kit (Agilent Technologies). mRNA-focused cDNA libraries were synthesized using the TruSeq RNA Sample Preparation Kit v2 (Illumina) using 1 µg of starting material at the IBIS/Université Laval Plate-forme d’Analyses Génomiques. .. Libraries were sequenced on one lane of an Illumina HiSeq 2500 (v1.9 single-end 100 bp) at the McGill University and Génome Québec Innovation Centre.

    Ethanol Precipitation:

    Article Title: Mycobacterium tuberculosis–induced IFN-β production requires cytosolic DNA and RNA sensing pathways
    Article Snippet: .. Total cellular RNA was isolated from infected cells using Qiagen RNeasy Mini Kit (cat. 74136; Qiagen), and cytosolic RNA from cytosolic fraction by ethanol precipitation. .. For cytosolic RNA, cells were washed with precold PBS three times and then resuspended in lysis buffer (20 mM Hepes [pH 7.4], 250 mM sucrose, 10 mM KCl, 2 mM MgCl2 , and 1 mM EDTA) plus RNase inhibitor (M0314; NEB) and disrupted with a syringe and 27 G needle by 25 strikes.

    Laser Capture Microdissection:

    Article Title: The effect of mutant SOD1 dismutase activity on non-cell autonomous degeneration in familial amyotrophic lateral sclerosis
    Article Snippet: To preserve the integrity of the RNA, section cutting and staining were carried out just before laser capture microdissection (Leica Microsystems, Wetzlar, Germany). .. Approximately 1200 MNs from the anterior horn of each mouse spinal cord were collected into 75 μl of RLT buffer (Qiagen RNeasy Mini Kit, QIAGEN, Valencia, CA), and RNA was isolated using an RNeasy Mini Kit (QIAGEN).

    Spectrophotometry:

    Article Title: Study of Carbon Nano-Tubes Effects on the Chondrogenesis of Human Adipose Derived Stem Cells in Alginate Scaffold
    Article Snippet: The resultant solution was centrifuged for 10 min at 1200 rpm and the derived cell pellet was used for the ribonucleic acid (RNA) extraction with RNeasy mini kit (Qiagen, Cat. No. .. The extent of derived RNA was measured by spectrophotometer (Biophotometer, Eppendorf) at 260/280 nm wave length.

    Article Title: RNAseq Analysis Highlights Specific Transcriptome Signatures of Yeast and Mycelial Growth Phases in the Dutch Elm Disease Fungus Ophiostoma novo-ulmi
    Article Snippet: .. Total RNA was extracted using the RNeasy Mini Kit (QIAGEN, yeast protocol) and quality control was assured using a spectrophotometer (NanoDrop ND-1000, Thermo Scientific) and a bioanalyzer (BioAnalyzer RNA 6000 Nano Kit, Agilent Technologies). .. Complementary DNA (cDNA) libraries were synthesized using the TruSeq RNA Sample Preparation Kit v2 (Illumina) with 1 μg of starting material per sample at the Plateforme d’Analyses Génomiques (IBIS/Université Laval).

    Article Title: Microarray Expression Analysis of the Main Inflorescence in Brassica napus
    Article Snippet: RNA extraction Using an RNeasy Mini Kit (Cat. 74124, Qiagen, Mississauga, ON) according to the manufacturer's recommendations, the total RNA of inflorescence primordia was extracted in biological triplicate, each consisting of three main inflorescence sections taken from three independent plants. .. The RNA yield and purity were determined spectrophotometrically with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, USA), and the intactness of RNA was verified by electrophoresis on a 1% agarose gel with 1×TBE running buffer at 70 V for 40 min. Purified total RNA was precipitated and re-suspended in DEPC-treated water to a final concentration of about 500 ng/µL.

    Article Title: Identification of a common reference gene pair for qPCR in human mesenchymal stromal cells from different tissue sources treated with VEGF
    Article Snippet: Nucleic acid extraction Total RNA was extracted using Qiagen RNeasy® Mini Kit (Qiagen Hamburg GmbH, Hamburg, Germany, cat.no. .. RNA purity was measured using NanoDrop® 1000 Spectrophotometer (Thermo Scientific, MA, USA), and the eluate was stored at −80°C.

    Concentration Assay:

    Article Title: The Ameliorative Effects of a Tocotrienol-Rich Fraction on the AGE-RAGE Axis and Hypertension in High-Fat-Diet-Fed Rats with Metabolic Syndrome
    Article Snippet: RNA Extraction and cDNA Synthesis Total RNA of the rWAT was isolated with Tri-RNA reagent (Favorgen, Ping-Tung, Taiwan) followed by the Qiagen RNeasy Mini kit (Qiagen, Hilden, Germany) to clean up the RNA sample, while that of the liver was extracted with Qiagen RNeasy Mini kit directly. .. The concentration and purity of the RNA were determined by measuring the absorbance at 260 nm and 280 nm with Infinite® 200 PRO (TECAN, Zürich, Switzerland).

    Article Title: Increased susceptibility of post-weaning rats on high-fat diet to metabolic syndrome
    Article Snippet: RNA extraction and cDNA synthesis Total RNA extraction of the liver was conducted using Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany), whereas that of rWAT was isolated with Tri-RNA reagent (Favorgen, Ping-Tung, Taiwan) and Qiagen RNeasy Mini Kit. .. The concentration and purity of the RNA were determined by measuring the absorbance at 260 nm and 280 nm with Infinite® 200 PRO (TECAN, Zürich, Switzerland).

    Article Title: Microarray Expression Analysis of the Main Inflorescence in Brassica napus
    Article Snippet: RNA extraction Using an RNeasy Mini Kit (Cat. 74124, Qiagen, Mississauga, ON) according to the manufacturer's recommendations, the total RNA of inflorescence primordia was extracted in biological triplicate, each consisting of three main inflorescence sections taken from three independent plants. .. The RNA yield and purity were determined spectrophotometrically with a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, USA), and the intactness of RNA was verified by electrophoresis on a 1% agarose gel with 1×TBE running buffer at 70 V for 40 min. Purified total RNA was precipitated and re-suspended in DEPC-treated water to a final concentration of about 500 ng/µL.

    Staining:

    Article Title: The effect of mutant SOD1 dismutase activity on non-cell autonomous degeneration in familial amyotrophic lateral sclerosis
    Article Snippet: In brief, the lumbar spinal cords were sectioned at 7 μm, mounted on glass-foil (Leica), and then immediatedly Nissl stained. .. Approximately 1200 MNs from the anterior horn of each mouse spinal cord were collected into 75 μl of RLT buffer (Qiagen RNeasy Mini Kit, QIAGEN, Valencia, CA), and RNA was isolated using an RNeasy Mini Kit (QIAGEN).

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    Qiagen rneasy plus mini kit
    Rneasy Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy mini kit
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 8038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy mini kit/product/Qiagen
    Average 90 stars, based on 8038 article reviews
    Price from $9.99 to $1999.99
    rneasy mini kit - by Bioz Stars, 2020-01
    90/100 stars
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