rneasy minelute column  (Qiagen)

 
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    Name:
    RNeasy MinElute Cleanup Kit
    Description:
    For RNA cleanup and concentration with small elution volumes Kit contents Qiagen RNeasy MinElute Cleanup Kit 50 preps 200L Sample 10 to 14L Elution Volume Crude RNA preps Sample Total RNA Purification Silica Technology MinElute Spin Column Format Ideal for PCR qPCR Real time PCR Microarray Includes 50 RNeasy MinElute Spin Columns Collection Tubes 1 5mL and 2mL RNase free Reagents and Buffers Benefits Efficient cleanup of enzymatic reactions Concentration of small amounts of RNA to only 10 µl Cleanup of RNA purified by different methods High quality RNA in less than 15 m
    Catalog Number:
    74204
    Price:
    348
    Category:
    RNeasy MinElute Cleanup Kit
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    Structured Review

    Qiagen rneasy minelute column
    RNeasy MinElute Cleanup Kit
    For RNA cleanup and concentration with small elution volumes Kit contents Qiagen RNeasy MinElute Cleanup Kit 50 preps 200L Sample 10 to 14L Elution Volume Crude RNA preps Sample Total RNA Purification Silica Technology MinElute Spin Column Format Ideal for PCR qPCR Real time PCR Microarray Includes 50 RNeasy MinElute Spin Columns Collection Tubes 1 5mL and 2mL RNase free Reagents and Buffers Benefits Efficient cleanup of enzymatic reactions Concentration of small amounts of RNA to only 10 µl Cleanup of RNA purified by different methods High quality RNA in less than 15 m
    https://www.bioz.com/result/rneasy minelute column/product/Qiagen
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    rneasy minelute column - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins
    Article Snippet: .. Quantitative Reverse Transcription-Polymerase Chain Reaction (Quantitative RT-PCR) Total RNA was extracted using the RNeasy MinElute Cleaning Kit (Qiagen), and the cDNAs were synthesized using the PrimeScript® RT Master Mix (Takara Bio) according to the manufacturer's specifications. .. Quantitative PCR analysis was performed using gene-specific primers (Table S4), the Power SYBR® Green PCR Master Mix, and the 7300 Real-Time PCR System (Applied Biosystems).

    Isolation:

    Article Title: Transcriptome Analysis of an Insecticide Resistant Housefly Strain: Insights about SNPs and Regulatory Elements in Cytochrome P450 Genes
    Article Snippet: .. Isolated RNA was DNase-treated and concentrated using the RNeasy MinElute Kit (Qiagen). .. Gel electrophoresis and spectrophotometry (Nanodrop; NanoDrop Technologies, Wilmington, USA) was performed to assess the integrity and the concentration of each RNA sample, which was dissolved in RNase-free water and stored at -20°C until use.

    Quantitative RT-PCR:

    Article Title: Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins
    Article Snippet: .. Quantitative Reverse Transcription-Polymerase Chain Reaction (Quantitative RT-PCR) Total RNA was extracted using the RNeasy MinElute Cleaning Kit (Qiagen), and the cDNAs were synthesized using the PrimeScript® RT Master Mix (Takara Bio) according to the manufacturer's specifications. .. Quantitative PCR analysis was performed using gene-specific primers (Table S4), the Power SYBR® Green PCR Master Mix, and the 7300 Real-Time PCR System (Applied Biosystems).

    Purification:

    Article Title: Restricted leucine zipper dimerization and specificity of DNA recognition of the melanocyte master regulator MITF
    Article Snippet: .. RNA was purified with the RNEasy Minelute kit (Qiagen). .. Purified RNA (2 μg in a 20-μL reaction) was used for cDNA synthesis with a High-Capacity cDNA reverse transcription kit (Applied Biosystems) and anchored Oligo(dT)20 primer (Invitrogen).

    Article Title: Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers
    Article Snippet: .. The associated feature with lowest p -value to the parvovirus clusters suggested a contamination from the RNeasy MinElute purification kit (f076) manufactured by Qiagen (p -value: 5.48e-38). .. In addition, a single cluster annotated as NCBI taxonomy species Acanthocystis turfacea Chlorella virus MN0810.1 (accession JX997174.1, taxa-id 1278272) with lowest associated p -value (p -value = 4.19e-12) to laboratory kit DNase/RNase: Promega DNase stop solution (f069).

    Article Title: Genome-wide meta-analysis of maize heterosis reveals the potential role of additive gene expression at pericentromeric loci
    Article Snippet: .. The RNeasy MinElute Kit (Qiagen, Hilden, Germany) was used for purification and removal of unbound dye. .. Microarray analysis Expression analysis of MPH-correlated genes was performed with a custom-made 1.5k-microarray (GEO Platform accession number GPL17936) with 732 genes printed in duplicates.

    Article Title: Transcriptome and Proteome Data Reveal Candidate Genes for Pollinator Attraction in Sexually Deceptive Orchids
    Article Snippet: .. Extracts were further purified by using RNeasy MinElute Cleanup columns (Qiagen) according to the manufacturer’s protocol. .. The extracted RNAs were analysed for potential degradation by gel electrophoresis and on a Bioanalyzer 2100 (Agilent), and RNA concentration was quantified using the fluorometric Qubit Quantitation Platform (Invitrogen).

    Significance Assay:

    Article Title: Identification of Known and Novel Recurrent Viral Sequences in Data from Multiple Patients and Multiple Cancers
    Article Snippet: .. The associated feature with lowest p -value to the parvovirus clusters suggested a contamination from the RNeasy MinElute purification kit (f076) manufactured by Qiagen (p -value: 5.48e-38). .. In addition, a single cluster annotated as NCBI taxonomy species Acanthocystis turfacea Chlorella virus MN0810.1 (accession JX997174.1, taxa-id 1278272) with lowest associated p -value (p -value = 4.19e-12) to laboratory kit DNase/RNase: Promega DNase stop solution (f069).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins
    Article Snippet: .. Quantitative Reverse Transcription-Polymerase Chain Reaction (Quantitative RT-PCR) Total RNA was extracted using the RNeasy MinElute Cleaning Kit (Qiagen), and the cDNAs were synthesized using the PrimeScript® RT Master Mix (Takara Bio) according to the manufacturer's specifications. .. Quantitative PCR analysis was performed using gene-specific primers (Table S4), the Power SYBR® Green PCR Master Mix, and the 7300 Real-Time PCR System (Applied Biosystems).

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    Qiagen rna affinity columns
    Functional and structural validation of newly discovered HIV-1 <t>RNA</t> motifs ( a ) Scheme for simultaneous deconvolution and structural analysis of a mixture of native sequence and U3 PK mutant genomes. (b) SHAPE profiles for the U3 PK pseudoknot bridging U3 and R. The experiment simultaneously probed a mixture of viruses with native sequence and mutant U3 PK <t>RNAs.</t> Secondary structure for the native sequence is shown as arcs below the y-axis intercept. Significant SHAPE reactivity differences are emphasized with yellow vertical lines (see Online Methods). (c) Direct growth competition and viral spread for U3 PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. Percentage of mutant in the initial inoculum is presented as a grey square at day 0. p24 levels correspond to the amount of HIV-1 capsid protein. ( d ) SHAPE profiles for the RT PK pseudoknot within the reverse transcriptase coding region. In this case, SHAPE data were obtained in separate experiments for each virus. ( e ) Viral spread and direct growth competition for RT PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. For the competition data, y-axes are shown on an expanded scale for clarity.
    Rna Affinity Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy minelute spin column
    Functional and structural validation of newly discovered HIV-1 <t>RNA</t> motifs ( a ) Scheme for simultaneous deconvolution and structural analysis of a mixture of native sequence and U3 PK mutant genomes. (b) SHAPE profiles for the U3 PK pseudoknot bridging U3 and R. The experiment simultaneously probed a mixture of viruses with native sequence and mutant U3 PK <t>RNAs.</t> Secondary structure for the native sequence is shown as arcs below the y-axis intercept. Significant SHAPE reactivity differences are emphasized with yellow vertical lines (see Online Methods). (c) Direct growth competition and viral spread for U3 PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. Percentage of mutant in the initial inoculum is presented as a grey square at day 0. p24 levels correspond to the amount of HIV-1 capsid protein. ( d ) SHAPE profiles for the RT PK pseudoknot within the reverse transcriptase coding region. In this case, SHAPE data were obtained in separate experiments for each virus. ( e ) Viral spread and direct growth competition for RT PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. For the competition data, y-axes are shown on an expanded scale for clarity.
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    Functional and structural validation of newly discovered HIV-1 <t>RNA</t> motifs ( a ) Scheme for simultaneous deconvolution and structural analysis of a mixture of native sequence and U3 PK mutant genomes. (b) SHAPE profiles for the U3 PK pseudoknot bridging U3 and R. The experiment simultaneously probed a mixture of viruses with native sequence and mutant U3 PK <t>RNAs.</t> Secondary structure for the native sequence is shown as arcs below the y-axis intercept. Significant SHAPE reactivity differences are emphasized with yellow vertical lines (see Online Methods). (c) Direct growth competition and viral spread for U3 PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. Percentage of mutant in the initial inoculum is presented as a grey square at day 0. p24 levels correspond to the amount of HIV-1 capsid protein. ( d ) SHAPE profiles for the RT PK pseudoknot within the reverse transcriptase coding region. In this case, SHAPE data were obtained in separate experiments for each virus. ( e ) Viral spread and direct growth competition for RT PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. For the competition data, y-axes are shown on an expanded scale for clarity.
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    Qiagen plant rneasy mini kit
    Functional and structural validation of newly discovered HIV-1 <t>RNA</t> motifs ( a ) Scheme for simultaneous deconvolution and structural analysis of a mixture of native sequence and U3 PK mutant genomes. (b) SHAPE profiles for the U3 PK pseudoknot bridging U3 and R. The experiment simultaneously probed a mixture of viruses with native sequence and mutant U3 PK <t>RNAs.</t> Secondary structure for the native sequence is shown as arcs below the y-axis intercept. Significant SHAPE reactivity differences are emphasized with yellow vertical lines (see Online Methods). (c) Direct growth competition and viral spread for U3 PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. Percentage of mutant in the initial inoculum is presented as a grey square at day 0. p24 levels correspond to the amount of HIV-1 capsid protein. ( d ) SHAPE profiles for the RT PK pseudoknot within the reverse transcriptase coding region. In this case, SHAPE data were obtained in separate experiments for each virus. ( e ) Viral spread and direct growth competition for RT PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. For the competition data, y-axes are shown on an expanded scale for clarity.
    Plant Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Functional and structural validation of newly discovered HIV-1 RNA motifs ( a ) Scheme for simultaneous deconvolution and structural analysis of a mixture of native sequence and U3 PK mutant genomes. (b) SHAPE profiles for the U3 PK pseudoknot bridging U3 and R. The experiment simultaneously probed a mixture of viruses with native sequence and mutant U3 PK RNAs. Secondary structure for the native sequence is shown as arcs below the y-axis intercept. Significant SHAPE reactivity differences are emphasized with yellow vertical lines (see Online Methods). (c) Direct growth competition and viral spread for U3 PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. Percentage of mutant in the initial inoculum is presented as a grey square at day 0. p24 levels correspond to the amount of HIV-1 capsid protein. ( d ) SHAPE profiles for the RT PK pseudoknot within the reverse transcriptase coding region. In this case, SHAPE data were obtained in separate experiments for each virus. ( e ) Viral spread and direct growth competition for RT PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. For the competition data, y-axes are shown on an expanded scale for clarity.

    Journal: Nature methods

    Article Title: RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP)

    doi: 10.1038/nmeth.3029

    Figure Lengend Snippet: Functional and structural validation of newly discovered HIV-1 RNA motifs ( a ) Scheme for simultaneous deconvolution and structural analysis of a mixture of native sequence and U3 PK mutant genomes. (b) SHAPE profiles for the U3 PK pseudoknot bridging U3 and R. The experiment simultaneously probed a mixture of viruses with native sequence and mutant U3 PK RNAs. Secondary structure for the native sequence is shown as arcs below the y-axis intercept. Significant SHAPE reactivity differences are emphasized with yellow vertical lines (see Online Methods). (c) Direct growth competition and viral spread for U3 PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. Percentage of mutant in the initial inoculum is presented as a grey square at day 0. p24 levels correspond to the amount of HIV-1 capsid protein. ( d ) SHAPE profiles for the RT PK pseudoknot within the reverse transcriptase coding region. In this case, SHAPE data were obtained in separate experiments for each virus. ( e ) Viral spread and direct growth competition for RT PK mutant and native sequence NL4-3 HIV-1 virions in Jurkat cells. For the competition data, y-axes are shown on an expanded scale for clarity.

    Article Snippet: Following modification, RNAs were isolated using either RNA affinity columns (RNeasy MinElute; Qiagen) or G-50 spin columns (GE Healthcare).

    Techniques: Functional Assay, Sequencing, Mutagenesis