rneasy minelute cleanup kit  (Qiagen)


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    Structured Review

    Qiagen rneasy minelute cleanup kit
    Rneasy Minelute Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 988 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy minelute cleanup kit/product/Qiagen
    Average 99 stars, based on 988 article reviews
    Price from $9.99 to $1999.99
    rneasy minelute cleanup kit - by Bioz Stars, 2020-01
    99/100 stars

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    Centrifugation:

    Article Title: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism
    Article Snippet: After 5 min at room temperature and centrifugation (1 min, 12,000× g , 4 °C), supernatants were transferred into a tube containing 300 μl chloroform (VWR, cat. no. 22711290), vortexed, incubated 3 min at room temperature, and centrifuged (15 min, 12,000× g , 4 °C). .. Total RNA was depleted from rRNA using the Ribo-Zero™ Bacteria Kit (Illumina, cat. no. MRZB12424) as recommended by the manufacturer. rRNA-depleted RNA was purified using the RNeasy MinElute CleanUp Kit (Qiagen, cat.no.

    RNA Sequencing Assay:

    Article Title: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism
    Article Snippet: Total RNA was depleted from rRNA using the Ribo-Zero™ Bacteria Kit (Illumina, cat. no. MRZB12424) as recommended by the manufacturer. rRNA-depleted RNA was purified using the RNeasy MinElute CleanUp Kit (Qiagen, cat.no. .. 74204). cDNA synthesis and library preparation were performed using the ScriptSeq™v2 RNA-Seq Library Preparation Kit (epicenter, cat. no. / ). cDNA was purified using the MinElute PCR Purification Kit (Qiagen, cat. no. 28004).

    RNA Extraction:

    Article Title: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism
    Article Snippet: RNA extraction was completed as described by the manufacturer including on-column DNA digestion using the RNase-free DNAse set (Qiagen, cat. no. 79254). .. Total RNA was depleted from rRNA using the Ribo-Zero™ Bacteria Kit (Illumina, cat. no. MRZB12424) as recommended by the manufacturer. rRNA-depleted RNA was purified using the RNeasy MinElute CleanUp Kit (Qiagen, cat.no.

    Purification:

    Article Title: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism
    Article Snippet: .. Total RNA was depleted from rRNA using the Ribo-Zero™ Bacteria Kit (Illumina, cat. no. MRZB12424) as recommended by the manufacturer. rRNA-depleted RNA was purified using the RNeasy MinElute CleanUp Kit (Qiagen, cat.no. .. 74204). cDNA synthesis and library preparation were performed using the ScriptSeq™v2 RNA-Seq Library Preparation Kit (epicenter, cat. no. / ). cDNA was purified using the MinElute PCR Purification Kit (Qiagen, cat. no. 28004).

    Incubation:

    Article Title: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism
    Article Snippet: After 5 min at room temperature and centrifugation (1 min, 12,000× g , 4 °C), supernatants were transferred into a tube containing 300 μl chloroform (VWR, cat. no. 22711290), vortexed, incubated 3 min at room temperature, and centrifuged (15 min, 12,000× g , 4 °C). .. Total RNA was depleted from rRNA using the Ribo-Zero™ Bacteria Kit (Illumina, cat. no. MRZB12424) as recommended by the manufacturer. rRNA-depleted RNA was purified using the RNeasy MinElute CleanUp Kit (Qiagen, cat.no.

    Polymerase Chain Reaction:

    Article Title: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism
    Article Snippet: Total RNA was depleted from rRNA using the Ribo-Zero™ Bacteria Kit (Illumina, cat. no. MRZB12424) as recommended by the manufacturer. rRNA-depleted RNA was purified using the RNeasy MinElute CleanUp Kit (Qiagen, cat.no. .. 74204). cDNA synthesis and library preparation were performed using the ScriptSeq™v2 RNA-Seq Library Preparation Kit (epicenter, cat. no. / ). cDNA was purified using the MinElute PCR Purification Kit (Qiagen, cat. no. 28004).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism
    Article Snippet: .. Total RNA was depleted from rRNA using the Ribo-Zero™ Bacteria Kit (Illumina, cat. no. MRZB12424) as recommended by the manufacturer. rRNA-depleted RNA was purified using the RNeasy MinElute CleanUp Kit (Qiagen, cat.no. .. 74204). cDNA synthesis and library preparation were performed using the ScriptSeq™v2 RNA-Seq Library Preparation Kit (epicenter, cat. no. / ). cDNA was purified using the MinElute PCR Purification Kit (Qiagen, cat. no. 28004).

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    Qiagen rneasy minelute cleanup kit
    Rneasy Minelute Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 988 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy minelute cleanup kit/product/Qiagen
    Average 90 stars, based on 988 article reviews
    Price from $9.99 to $1999.99
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    90/100 stars
      Buy from Supplier

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