rneasy micro plus kit  (Qiagen)

 
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    Name:
    RNeasy Micro Kit
    Description:
    For purification of up to 45 µg total RNA from cell and tissue samples Kit contents Qiagen RNeasy Micro Kit 50 preps 10 to 14L Elution Volume 5mg Sample Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes 50 RNeasy MinElute Spin Columns Collection Tubes 1 5mL and 2mL RNase free DNase I Carrier RNA RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from very small amounts of starting material No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation
    Catalog Number:
    74004
    Price:
    492
    Category:
    RNeasy Micro Kit
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    Structured Review

    Qiagen rneasy micro plus kit
    RNeasy Micro Kit
    For purification of up to 45 µg total RNA from cell and tissue samples Kit contents Qiagen RNeasy Micro Kit 50 preps 10 to 14L Elution Volume 5mg Sample Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes 50 RNeasy MinElute Spin Columns Collection Tubes 1 5mL and 2mL RNase free DNase I Carrier RNA RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from very small amounts of starting material No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation
    https://www.bioz.com/result/rneasy micro plus kit/product/Qiagen
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    rneasy micro plus kit - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. Ten days post-differentiation, EBs in the supernatant were harvested by centrifugation (BeckmanAllegra-6R, 1000 rpm, 5 min) and RNA was isolated using the RNeasy Micro Kit (Qiagen). .. Total RNA (500 ng) was reverse-transcribed using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 and used as template in subsequent PCR with Taq DNA Polymerase.

    Isolation:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. Ten days post-differentiation, EBs in the supernatant were harvested by centrifugation (BeckmanAllegra-6R, 1000 rpm, 5 min) and RNA was isolated using the RNeasy Micro Kit (Qiagen). .. Total RNA (500 ng) was reverse-transcribed using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 and used as template in subsequent PCR with Taq DNA Polymerase.

    Article Title: Global Array-Based Transcriptomics from Minimal Input RNA Utilising an Optimal RNA Isolation Process Combined with SPIA cDNA Probes
    Article Snippet: .. Total RNA from minimal cell numbers was also extracted using the RNeasy Micro RNA isolation kit with the following modifications (process B) ( ). ..

    Article Title: Non‐invasive lung cancer diagnosis by detection of GATA6 and NKX2‐1 isoforms in exhaled breath condensate
    Article Snippet: .. Total RNA was isolated using the RNeasy Micro Kit (Qiagen), and isoform‐specific expression analysis was performed as explained above. .. Total protein extracts from control and LC snap‐frozen tissue samples were analyzed by Western blotting following standard protocols (Singh et al , ) and using antibodies specific for CD63 (ab8219, Abcam), TSG101 (sc‐7964, Santa Cruz), and ACTB (ab6276, Abcam).

    Article Title: Non‐invasive lung cancer diagnosis by detection of GATA6 and NKX2‐1 isoforms in exhaled breath condensate
    Article Snippet: .. Total RNA isolation from EBC was performed using 500 μl of sample and the RNeasy Micro Kit (Qiagen). .. Complementary DNA (cDNA) was synthetized using the High Capacity cDNA Reverse Transcription kit (Applied Biosystem) with 0.5–0.7 μg (EBC) or 1 μg (FFPE sample) total RNA.

    Article Title: Global Array-Based Transcriptomics from Minimal Input RNA Utilising an Optimal RNA Isolation Process Combined with SPIA cDNA Probes
    Article Snippet: .. RNA isolation from vascular endothelial biopsies was also carried out using the RNeasy Micro RNA isolation kit (Qiagen) following process B ( ). ..

    Spectrophotometry:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. RT-PCR and PCR Total RNA was harvested using RNeasy Micro Kit (Qiagen) and quantified by spectrophotometer. .. 500 ng of RNA was used for cDNA synthesis using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 (Invitrogen).

    Purification:

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq
    Article Snippet: .. One volume of freshly prepared RNase-free 70% ethanol was added to the lysate and total RNA purification was performed by following RNeasy® Micro user guide (QIAGEN, Cat #74004). .. RNase-free DNase set (QIAGEN, Cat#79254) was utilized to remove genomic DNA that can interfere with downstream applications.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. RT-PCR and PCR Total RNA was harvested using RNeasy Micro Kit (Qiagen) and quantified by spectrophotometer. .. 500 ng of RNA was used for cDNA synthesis using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 (Invitrogen).

    Expressing:

    Article Title: Non‐invasive lung cancer diagnosis by detection of GATA6 and NKX2‐1 isoforms in exhaled breath condensate
    Article Snippet: .. Total RNA was isolated using the RNeasy Micro Kit (Qiagen), and isoform‐specific expression analysis was performed as explained above. .. Total protein extracts from control and LC snap‐frozen tissue samples were analyzed by Western blotting following standard protocols (Singh et al , ) and using antibodies specific for CD63 (ab8219, Abcam), TSG101 (sc‐7964, Santa Cruz), and ACTB (ab6276, Abcam).

    Polymerase Chain Reaction:

    Article Title: Antibody-Directed Lentiviral Gene Transduction for Live-Cell Monitoring and Selection of Human iPS and hES Cells
    Article Snippet: .. RT-PCR and PCR Total RNA was harvested using RNeasy Micro Kit (Qiagen) and quantified by spectrophotometer. .. 500 ng of RNA was used for cDNA synthesis using Superscript III Reverse Transcriptase primed with oligo(dT)12–18 (Invitrogen).

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    Qiagen rna column based isolation kits
    Islet1 knock-down affects sensory neuron differentiation. (A-C) In control embryos (A, B) , Rohon-Beard cells (RBs) express runx3 . Islet1 knock-down leads to fewer cells with robust expression of runx3 (C). The asterisk denotes a cell expressing the gene. (D-F’) <t>Tg(-3.4neurog1:gfp)sb4</t> control (D-E’) and E3 morphant (F, F’) 24 hours post-fertilization (hpf) embryos were examined for expression of olig4 (red). (D-E’) In lateral views of the dorsal spinal cord, <t>RNA</t> in situ hybridization reveals expression of the interneuron marker olig4 (red). Green fluorescent protein (GFP) + neurons do not express olig4 and comprise RBs and dorsal lateral ascending interneurons (see Figure 2 ). (F-F’) Islet1 knock-down leads to an increase in the number of olig4 expressing cells within the dorsal spinal cord. However, similar to RBs of control embryos (D-E’) , RB-like neurons do not express detectable levels of olig4 (F) . (G-I) At 72 hpf, dorsal root ganglia (DRGs) are easily identified as GFP + neurons with large somata located near the spinal cord/notochord border. (G, H) In control embryos, DRG neurons project from their soma bipolar axons that extend dorsally and ventrally (asterisks). (I) Islet1 knock-down reduces the number of GFP + DRGs. Furthermore, for the few GFP + DRGs remaining, their axons show abnormal morphologies. Scale bars = 50 μm in A (for A-C ), D (for D-F ’) and G (for G-I ). CtlMO, 5-base mismatched; islet1 (Sp)E3MO; E3MO, E3 morpholino; Uninj, uninjected.
    Rna Column Based Isolation Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna column based isolation kits/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rna column based isolation kits - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Qiagen rneasy kit
    A, Effect of POU1F1 R271W on Icer and Crem mRNA expression in GH4C1 cells. Total RNA from cells infected with the <t>lentiviral</t> vectors (with or without the transgene) was isolated by using the <t>RNeasy</t> Kit (QIAGEN), and reverse transcription was performed
    Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2846 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy kit/product/Qiagen
    Average 99 stars, based on 2846 article reviews
    Price from $9.99 to $1999.99
    rneasy kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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    Islet1 knock-down affects sensory neuron differentiation. (A-C) In control embryos (A, B) , Rohon-Beard cells (RBs) express runx3 . Islet1 knock-down leads to fewer cells with robust expression of runx3 (C). The asterisk denotes a cell expressing the gene. (D-F’) Tg(-3.4neurog1:gfp)sb4 control (D-E’) and E3 morphant (F, F’) 24 hours post-fertilization (hpf) embryos were examined for expression of olig4 (red). (D-E’) In lateral views of the dorsal spinal cord, RNA in situ hybridization reveals expression of the interneuron marker olig4 (red). Green fluorescent protein (GFP) + neurons do not express olig4 and comprise RBs and dorsal lateral ascending interneurons (see Figure 2 ). (F-F’) Islet1 knock-down leads to an increase in the number of olig4 expressing cells within the dorsal spinal cord. However, similar to RBs of control embryos (D-E’) , RB-like neurons do not express detectable levels of olig4 (F) . (G-I) At 72 hpf, dorsal root ganglia (DRGs) are easily identified as GFP + neurons with large somata located near the spinal cord/notochord border. (G, H) In control embryos, DRG neurons project from their soma bipolar axons that extend dorsally and ventrally (asterisks). (I) Islet1 knock-down reduces the number of GFP + DRGs. Furthermore, for the few GFP + DRGs remaining, their axons show abnormal morphologies. Scale bars = 50 μm in A (for A-C ), D (for D-F ’) and G (for G-I ). CtlMO, 5-base mismatched; islet1 (Sp)E3MO; E3MO, E3 morpholino; Uninj, uninjected.

    Journal: Neural Development

    Article Title: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability

    doi: 10.1186/1749-8104-9-19

    Figure Lengend Snippet: Islet1 knock-down affects sensory neuron differentiation. (A-C) In control embryos (A, B) , Rohon-Beard cells (RBs) express runx3 . Islet1 knock-down leads to fewer cells with robust expression of runx3 (C). The asterisk denotes a cell expressing the gene. (D-F’) Tg(-3.4neurog1:gfp)sb4 control (D-E’) and E3 morphant (F, F’) 24 hours post-fertilization (hpf) embryos were examined for expression of olig4 (red). (D-E’) In lateral views of the dorsal spinal cord, RNA in situ hybridization reveals expression of the interneuron marker olig4 (red). Green fluorescent protein (GFP) + neurons do not express olig4 and comprise RBs and dorsal lateral ascending interneurons (see Figure 2 ). (F-F’) Islet1 knock-down leads to an increase in the number of olig4 expressing cells within the dorsal spinal cord. However, similar to RBs of control embryos (D-E’) , RB-like neurons do not express detectable levels of olig4 (F) . (G-I) At 72 hpf, dorsal root ganglia (DRGs) are easily identified as GFP + neurons with large somata located near the spinal cord/notochord border. (G, H) In control embryos, DRG neurons project from their soma bipolar axons that extend dorsally and ventrally (asterisks). (I) Islet1 knock-down reduces the number of GFP + DRGs. Furthermore, for the few GFP + DRGs remaining, their axons show abnormal morphologies. Scale bars = 50 μm in A (for A-C ), D (for D-F ’) and G (for G-I ). CtlMO, 5-base mismatched; islet1 (Sp)E3MO; E3MO, E3 morpholino; Uninj, uninjected.

    Article Snippet: Real time quantitative PCR Total RNA was extracted from FAC sorted GFP+ cells using RNA column-based isolation kits, RNeasy® Micro kit (Qiagen).

    Techniques: Expressing, RNA In Situ Hybridization, Marker

    A, Effect of POU1F1 R271W on Icer and Crem mRNA expression in GH4C1 cells. Total RNA from cells infected with the lentiviral vectors (with or without the transgene) was isolated by using the RNeasy Kit (QIAGEN), and reverse transcription was performed

    Journal: Molecular Endocrinology

    Article Title: Research Resource: A Genome-Wide Study Identifies Potential New Target Genes for POU1F1

    doi: 10.1210/me.2011-1308

    Figure Lengend Snippet: A, Effect of POU1F1 R271W on Icer and Crem mRNA expression in GH4C1 cells. Total RNA from cells infected with the lentiviral vectors (with or without the transgene) was isolated by using the RNeasy Kit (QIAGEN), and reverse transcription was performed

    Article Snippet: Total RNA from cells infected with the lentiviral vectors was isolated by using the RNeasy Kit (Qiagen, Hilden, Germany), including on-column DNase I digestion according to the manufacturer's recommendations.

    Techniques: Expressing, Infection, Isolation

    Quantitative RT-PCR analysis of PAI-1 mRNA levels in patients. The total RNA was harvested from the peripheral blood mononuclear cells using an RNeasy kit according to the manufacturer’s instructions. The RT-PCR experiments were repeated at least 3 times. RNA was reverse transcribed into cDNA using random primers in a Reverse Transcription II system according to the manufacturer’s instructions. The expression of PAI-1 mRNA was quantified by quantitative PCR using an ABI Prism Sequence Detection System. Template-negative and RT-negative conditions were used as controls. Amplification of the endogenous GAPDH cDNA was monitored. The levels (mean value) of PAI-1 transcripts in patients were calculated. RT-PCR, reverse-transcription-PCR, PAI-1, plasminogen activator inhibitor-1.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Therapeutic effects of calcium dobesilate on diabetic nephropathy mediated through reduction of expression of PAI-1

    doi: 10.3892/etm.2012.755

    Figure Lengend Snippet: Quantitative RT-PCR analysis of PAI-1 mRNA levels in patients. The total RNA was harvested from the peripheral blood mononuclear cells using an RNeasy kit according to the manufacturer’s instructions. The RT-PCR experiments were repeated at least 3 times. RNA was reverse transcribed into cDNA using random primers in a Reverse Transcription II system according to the manufacturer’s instructions. The expression of PAI-1 mRNA was quantified by quantitative PCR using an ABI Prism Sequence Detection System. Template-negative and RT-negative conditions were used as controls. Amplification of the endogenous GAPDH cDNA was monitored. The levels (mean value) of PAI-1 transcripts in patients were calculated. RT-PCR, reverse-transcription-PCR, PAI-1, plasminogen activator inhibitor-1.

    Article Snippet: The total RNA was harvested from peripheral blood mononuclear cells using the RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction, Sequencing, Amplification, Polymerase Chain Reaction

    2′- O methylation of internal adenosine. (A) Incorporation of 3 H-methyl into polyA. Homopolymer RNAs (1 µg) were incubated with 2 µg of DENV-4 MTase in the presence of [ 3 H-methyl]-SAM. After the methylation reaction, the un-incorporated [ 3 H-methyl]-SAM was removed by RNeasy kit. The amount of 3 H-methyl incorporation was measured by a MicroBeta counting. (B) SPA-based methylation analysis of oligo (A) 12 , (Am) 12 , and (m 6 ,m 6 A) 12 . All three RNA oligos were 3′-end biotinylated to facilitate SPA analysis. Am indicates that the 2′-OH of adenosine is methylated. m 6 ,m 6 A indicates that the amino N 6 position of adenosine is double methylated. (C) SPA-based methylation analysis of DENV-1 RNA. pppA-RNAs, representing the 5′ 211 nt of DENV-1 genome, were in vitro transcribed using biotinylated-CTP plus unmodified ATP, 2′- O -methyladenosine triphosphate (AmTP), or N 6 methyl adenosine triphosphate (m 6 ATP). The transcription reactions generated pppA-RNA, ppp(Am)-RNA, and ppp(m 6 A)RNA, respectively. The RNAs were then subjected to SPA-based internal methylation analysis. Average results and standard deviations from three independent experiments are presented.

    Journal: PLoS Pathogens

    Article Title: 2?-O Methylation of Internal Adenosine by Flavivirus NS5 Methyltransferase

    doi: 10.1371/journal.ppat.1002642

    Figure Lengend Snippet: 2′- O methylation of internal adenosine. (A) Incorporation of 3 H-methyl into polyA. Homopolymer RNAs (1 µg) were incubated with 2 µg of DENV-4 MTase in the presence of [ 3 H-methyl]-SAM. After the methylation reaction, the un-incorporated [ 3 H-methyl]-SAM was removed by RNeasy kit. The amount of 3 H-methyl incorporation was measured by a MicroBeta counting. (B) SPA-based methylation analysis of oligo (A) 12 , (Am) 12 , and (m 6 ,m 6 A) 12 . All three RNA oligos were 3′-end biotinylated to facilitate SPA analysis. Am indicates that the 2′-OH of adenosine is methylated. m 6 ,m 6 A indicates that the amino N 6 position of adenosine is double methylated. (C) SPA-based methylation analysis of DENV-1 RNA. pppA-RNAs, representing the 5′ 211 nt of DENV-1 genome, were in vitro transcribed using biotinylated-CTP plus unmodified ATP, 2′- O -methyladenosine triphosphate (AmTP), or N 6 methyl adenosine triphosphate (m 6 ATP). The transcription reactions generated pppA-RNA, ppp(Am)-RNA, and ppp(m 6 A)RNA, respectively. The RNAs were then subjected to SPA-based internal methylation analysis. Average results and standard deviations from three independent experiments are presented.

    Article Snippet: For each time point, total cellular RNA was extracted using RNeasy kit (Qiagen).

    Techniques: Methylation, Incubation, In Vitro, Generated