rneasy micro kit  (Qiagen)


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    Structured Review

    Qiagen rneasy micro kit
    Rneasy Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy micro kit/product/Qiagen
    Average 99 stars, based on 2018 article reviews
    Price from $9.99 to $1999.99
    rneasy micro kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Transcriptome dynamics of the stomatal lineage: birth, amplification and termination of a self-renewing population
    Article Snippet: .. Positive events were sorted directly into 350 ml RNA extraction buffer (RNeasy™ Micro Kit, QIAGEN) and total RNA of extracted with the RNeasy™ Micro Kit (QIAGEN) including on column DNase treatment. .. cDNA libraries for high throughput sequencing were prepared from ~10 ng of total RNA from each cell sample. cDNA was generated using the PrepX SPIA RNA-Seq Kit and libraries were generated using the ILM DNA Library Kit (Wafergen) according to the manufacturer’s protocol.

    Article Title: Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
    Article Snippet: RNA isolation and microarray hybridization The following samples were collected and processed for RNA extraction ( ): (1) epithelial cells from the BR monoculture; (2) epithelial cells and fibroblasts from the BRF coculture; (3) bronchial epithelial cells that were isolated/scraped from the exposed BRF co-culture (henceforth, they will be referred to as scraped bronchial epithelial samples [sBR]). .. Extraction and purification of the mRNAs and microRNAs were performed using a RNA with RNeasy micro kit (Qiagen, Cat. No. 74004) RNA with RNeasy micro kit (Cat. No. 74004 Qiagen).

    RNA Sequencing Assay:

    Article Title: Transcriptome dynamics of the stomatal lineage: birth, amplification and termination of a self-renewing population
    Article Snippet: Protoplast signals gated for RNA sequencing analysis are shown in . .. Positive events were sorted directly into 350 ml RNA extraction buffer (RNeasy™ Micro Kit, QIAGEN) and total RNA of extracted with the RNeasy™ Micro Kit (QIAGEN) including on column DNase treatment.

    Article Title: Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells
    Article Snippet: .. RNA-Seq assay Total RNA from NPCs was extracted using the RNeasy Micro Kit (Qiagen, 74004), treated with TURBO DNase (Ambion, AM2238) for 1 h at 37 °C, and then re-purified with the Qiagen RNeasy Micro Kit. .. RNA samples were quantified using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Q32852); purity was evaluated using NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies) and the integrity was verified using the Agilent RNA 6000 Pico Kit (Agilent Technologies, 5067-1513) in the 2100 Bioanalyzer Instrument (Agilent Technologies).

    RNA HS Assay:

    Article Title: Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells
    Article Snippet: RNA-Seq assay Total RNA from NPCs was extracted using the RNeasy Micro Kit (Qiagen, 74004), treated with TURBO DNase (Ambion, AM2238) for 1 h at 37 °C, and then re-purified with the Qiagen RNeasy Micro Kit. .. RNA samples were quantified using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Q32852); purity was evaluated using NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies) and the integrity was verified using the Agilent RNA 6000 Pico Kit (Agilent Technologies, 5067-1513) in the 2100 Bioanalyzer Instrument (Agilent Technologies).

    Microarray:

    Article Title: Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
    Article Snippet: Paragraph title: RNA isolation and microarray hybridization ... Extraction and purification of the mRNAs and microRNAs were performed using a RNA with RNeasy micro kit (Qiagen, Cat. No. 74004) RNA with RNeasy micro kit (Cat. No. 74004 Qiagen).

    Isolation:

    Article Title: Transcriptome dynamics of the stomatal lineage: birth, amplification and termination of a self-renewing population
    Article Snippet: Protoplast isolation and FACS from reporter line seedlings were performed as described in ( ) for FACS on a FACSAria II (BD Biosciences) fitted with a 100 μm nozzle. .. Positive events were sorted directly into 350 ml RNA extraction buffer (RNeasy™ Micro Kit, QIAGEN) and total RNA of extracted with the RNeasy™ Micro Kit (QIAGEN) including on column DNase treatment.

    Article Title: Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
    Article Snippet: Paragraph title: RNA isolation and microarray hybridization ... Extraction and purification of the mRNAs and microRNAs were performed using a RNA with RNeasy micro kit (Qiagen, Cat. No. 74004) RNA with RNeasy micro kit (Cat. No. 74004 Qiagen).

    Spectrophotometry:

    Article Title: Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells
    Article Snippet: RNA-Seq assay Total RNA from NPCs was extracted using the RNeasy Micro Kit (Qiagen, 74004), treated with TURBO DNase (Ambion, AM2238) for 1 h at 37 °C, and then re-purified with the Qiagen RNeasy Micro Kit. .. RNA samples were quantified using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Q32852); purity was evaluated using NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies) and the integrity was verified using the Agilent RNA 6000 Pico Kit (Agilent Technologies, 5067-1513) in the 2100 Bioanalyzer Instrument (Agilent Technologies).

    Article Title: Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
    Article Snippet: Extraction and purification of the mRNAs and microRNAs were performed using a RNA with RNeasy micro kit (Qiagen, Cat. No. 74004) RNA with RNeasy micro kit (Cat. No. 74004 Qiagen). .. The concentration of the isolated RNAs was measured by a NanoDrop ND1000 Spectrophotometer (Thermo Scientific, Waltham, Massachusetts).

    Purification:

    Article Title: Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
    Article Snippet: .. Extraction and purification of the mRNAs and microRNAs were performed using a RNA with RNeasy micro kit (Qiagen, Cat. No. 74004) RNA with RNeasy micro kit (Cat. No. 74004 Qiagen). .. The concentration of the isolated RNAs was measured by a NanoDrop ND1000 Spectrophotometer (Thermo Scientific, Waltham, Massachusetts).

    Concentration Assay:

    Article Title: Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
    Article Snippet: Extraction and purification of the mRNAs and microRNAs were performed using a RNA with RNeasy micro kit (Qiagen, Cat. No. 74004) RNA with RNeasy micro kit (Cat. No. 74004 Qiagen). .. The concentration of the isolated RNAs was measured by a NanoDrop ND1000 Spectrophotometer (Thermo Scientific, Waltham, Massachusetts).

    Co-Culture Assay:

    Article Title: Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
    Article Snippet: RNA isolation and microarray hybridization The following samples were collected and processed for RNA extraction ( ): (1) epithelial cells from the BR monoculture; (2) epithelial cells and fibroblasts from the BRF coculture; (3) bronchial epithelial cells that were isolated/scraped from the exposed BRF co-culture (henceforth, they will be referred to as scraped bronchial epithelial samples [sBR]). .. Extraction and purification of the mRNAs and microRNAs were performed using a RNA with RNeasy micro kit (Qiagen, Cat. No. 74004) RNA with RNeasy micro kit (Cat. No. 74004 Qiagen).

    FACS:

    Article Title: Transcriptome dynamics of the stomatal lineage: birth, amplification and termination of a self-renewing population
    Article Snippet: Paragraph title: FACS and RNA extraction ... Positive events were sorted directly into 350 ml RNA extraction buffer (RNeasy™ Micro Kit, QIAGEN) and total RNA of extracted with the RNeasy™ Micro Kit (QIAGEN) including on column DNase treatment.

    Sequencing:

    Article Title: Discordant congenital Zika syndrome twins show differential in vitro viral susceptibility of neural progenitor cells
    Article Snippet: RNA-Seq assay Total RNA from NPCs was extracted using the RNeasy Micro Kit (Qiagen, 74004), treated with TURBO DNase (Ambion, AM2238) for 1 h at 37 °C, and then re-purified with the Qiagen RNeasy Micro Kit. .. Tagged libraries were pooled and sequenced (300 cycles, paired-end sequencing) in the Illumina HiSeq 4000 instrument using a HiSeq 4000 SBS Kit (Illumina, FC-410-1003).

    Lysis:

    Article Title: Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
    Article Snippet: Briefly, the tissue inserts were washed 3 times with ice-cold PBS and lysed using RLT lysis buffer containing 1% β-mercaptoethanol (Cat. No. 79216; Qiagen, Venlo, The Netherlands) and then stored at −80°C until further processing. .. Extraction and purification of the mRNAs and microRNAs were performed using a RNA with RNeasy micro kit (Qiagen, Cat. No. 74004) RNA with RNeasy micro kit (Cat. No. 74004 Qiagen).

    Hybridization:

    Article Title: Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing Fibroblasts
    Article Snippet: Paragraph title: RNA isolation and microarray hybridization ... Extraction and purification of the mRNAs and microRNAs were performed using a RNA with RNeasy micro kit (Qiagen, Cat. No. 74004) RNA with RNeasy micro kit (Cat. No. 74004 Qiagen).

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    Qiagen rneasy plus mini kit
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