rneasy maxi kit  (Qiagen)


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    RNeasy Maxi Kit
    Description:
    For purification of up to 6 mg total RNA from cells tissues and yeast Kit contents Qiagen RNeasy Maxi Kit 800 to 2400L Elution Volume 150mg to 1g Sample 1 hr Time prep Spin Column Format Silica Technology Manual Processing Ideal for PCR qPCR Real time RT PCR Microarray Applications For Purification of up to 6mg Total RNA from Cells Tissues and Yeast Includes 12 RNeasy Maxi Spin Columns Collection Tubes 50mL RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from large amounts of starting material No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation
    Catalog Number:
    75162
    Price:
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    RNeasy Maxi Kit
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    Structured Review

    Qiagen rneasy maxi kit
    RNeasy Maxi Kit
    For purification of up to 6 mg total RNA from cells tissues and yeast Kit contents Qiagen RNeasy Maxi Kit 800 to 2400L Elution Volume 150mg to 1g Sample 1 hr Time prep Spin Column Format Silica Technology Manual Processing Ideal for PCR qPCR Real time RT PCR Microarray Applications For Purification of up to 6mg Total RNA from Cells Tissues and Yeast Includes 12 RNeasy Maxi Spin Columns Collection Tubes 50mL RNase free Reagents and Buffers Benefits Fast procedure delivering high quality total RNA in minutes Ready to use RNA for high performance in any downstream application Consistent RNA yields from large amounts of starting material No phenol chloroform extraction No CsCl gradients no LiCl or ethanol precipitation
    https://www.bioz.com/result/rneasy maxi kit/product/Qiagen
    Average 99 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    rneasy maxi kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A Novel Xylosylphosphotransferase Activity Discovered in Cryptococcus neoformans *
    Article Snippet: The plasmid contains two opposing pairs of promoters and terminators cloned from the GAL7 locus (NCBI accession number ) flanking a ∼250-bp fragment of the URA5 gene (NCBI accession number ). .. The upper aqueous phase was mixed with an equal volume of 70% ethanol and applied to an RNeasy® Maxi column (RNeasy® Maxi Kit, Qiagen).

    Centrifugation:

    Article Title: Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation
    Article Snippet: RNA preparation RNA was extracted using Trizol reagent (Invitrogen) and RNeasy maxi kit (Qiagen). .. RNA samples were concentrated to greater than 1 mg/ml by centrifugation on a MicroCon 100 filter unit at 500 g. The concentration and 260/280 OD ratio of the RNA was determined by spectrophotometry and RNA was stored at −80°C.

    Article Title: A Novel Xylosylphosphotransferase Activity Discovered in Cryptococcus neoformans *
    Article Snippet: To isolate total RNA, an overnight culture of the C. neoformans strain JEC21 was harvested by centrifugation (3,000 × g ; 10 min) and washed with 50 ml of diethylpyrocarbonate-treated water; the pellet was frozen in a dry ice/methanol bath and lyophilized overnight. .. The upper aqueous phase was mixed with an equal volume of 70% ethanol and applied to an RNeasy® Maxi column (RNeasy® Maxi Kit, Qiagen).

    Luciferase:

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification
    Article Snippet: Chemically modified, co-transcriptionally capped Cap 1 Cas9 and firefly luciferase (FLuc) mRNAs were synthesized by T7 RNA polymerase in vitro transcription. .. We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy.

    Synthesized:

    Article Title: ?-Synuclein Increases the Cellular Level of Phospholipase C?1
    Article Snippet: Total RNA was extracted from the cell lysate using the RNeasy Maxi Kit (Qiagen, Valencia, CA) with DNAse I treatment to eliminate genomic DNA contamination. .. First strand cDNA was synthesized from 5µg total RNA using the Superscript III Reverse Transcriptase (Invitrogen, Carlsbad, CA).

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification
    Article Snippet: Chemically modified, co-transcriptionally capped Cap 1 Cas9 and firefly luciferase (FLuc) mRNAs were synthesized by T7 RNA polymerase in vitro transcription. .. We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy.

    Quantitative RT-PCR:

    Article Title: Inflammatory Proprotein Convertase-Matrix Metalloproteinase Proteolytic Pathway in Antigen-presenting Cells as a Step to Autoimmune Multiple Sclerosis *
    Article Snippet: Paragraph title: Quantitative RT-PCR (Q-RT-PCR) ... Total RNA was isolated from the cells using the RNeasy Maxi kit (Qiagen).

    SYBR Green Assay:

    Article Title: ?-Synuclein Increases the Cellular Level of Phospholipase C?1
    Article Snippet: Total RNA was extracted from the cell lysate using the RNeasy Maxi Kit (Qiagen, Valencia, CA) with DNAse I treatment to eliminate genomic DNA contamination. .. The cDNA samples were diluted 1:16 and real-time PCR was performed using the QuantiTect SyBr Green PCR kit (Qiagen, Valencia, CA) in an ABI 7300 Real Time PCR System (Applied Biosystems,).

    Article Title: Inflammatory Proprotein Convertase-Matrix Metalloproteinase Proteolytic Pathway in Antigen-presenting Cells as a Step to Autoimmune Multiple Sclerosis *
    Article Snippet: Total RNA was isolated from the cells using the RNeasy Maxi kit (Qiagen). .. The cDNA (25 ng) and the primers (400 n m ) were used in the duplicate 30-μl reactions with the 1× SYBR Green PCR Master Mix (Applied Biosystems).

    Incubation:

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification
    Article Snippet: Transcription reactions were incubated at 37°C for 2 hr and treated with final 0.4 U/μL DNase I (M0303L) in 1× DNase I buffer for 15 min at 37°C. .. We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy.

    Article Title: Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation
    Article Snippet: In another set of experiments, control cells were incubated in DIP differentiation media for 72 h, while experimental cells were incubated in DIP differentiation media except that EGF was present. .. RNA preparation RNA was extracted using Trizol reagent (Invitrogen) and RNeasy maxi kit (Qiagen).

    Article Title: A Novel Xylosylphosphotransferase Activity Discovered in Cryptococcus neoformans *
    Article Snippet: Four milliliters of 0.5-mm glass beads were added to the dried pellet, and the sample was beaten to a fine powder using a vortex mixer, mixed with 4 ml of TRIzol® (Invitrogen), and incubated at room temperature for 5 min. Next, the sample was mixed with 800 μl of chloroform, incubated at room temperature for 3 min, and centrifuged at 3,000 × g for 30 min. .. The upper aqueous phase was mixed with an equal volume of 70% ethanol and applied to an RNeasy® Maxi column (RNeasy® Maxi Kit, Qiagen).

    Article Title: Phenotypic and genetic characterization of a novel phenotype in pigs characterized by juvenile hairlessness and age dependent emphysema
    Article Snippet: ITGB6 coding sequence Total RNA was isolated from lung tissue samples from two hh piglets using Qiagen (Germany) RNeasy Maxi kit according to the supplier's instructions. .. Four micrograms of the total RNA were incubated for 30 min at 37°C with RQ1 RNase-Free Dnase (Promega, USA) in a 20-μl reaction.

    Expressing:

    Article Title: Exosomes harbor B cell targets in pancreatic adenocarcinoma and exert decoy function against complement-mediated cytotoxicity
    Article Snippet: Paragraph title: Gene expression analysis ... mRNA extraction was performed by using RNeasy Maxi Kit (Qiagen).

    Article Title: Genome-wide distribution of Auts2 binding localizes with active neurodevelopmental genes
    Article Snippet: RNA-seq Total RNA was extracted from two replicates of E16.5 mouse forebrain tissue and purified using the RNeasy Maxi Kit (Qiagen, Venlo, Limburg, The Netherlands) and sent to Otogenetics for ribosomal RNA depletion, cDNA production using random primers, library preparation, and pair end sequencing (~100 bp) using Illumina HiSeq2000. .. Expression of each transcript was quantified as fragments per kilobase of transcript per million fragments mapped (FPKM) by dividing the total number of reads mapping to each transcript by transcript length, and the total number of reads aligned to the genome divided by one million.

    Modification:

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification
    Article Snippet: Paragraph title: In Vitro Transcription of Modified mRNAs ... We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy.

    Hybridization:

    Article Title: Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation
    Article Snippet: RNA preparation RNA was extracted using Trizol reagent (Invitrogen) and RNeasy maxi kit (Qiagen). .. Labeling, hybridization and analysis Mouse (Development) Oligo Microarrays were purchased from Agilent Technologies.

    High Performance Liquid Chromatography:

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification
    Article Snippet: We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy. .. A portion of each mRNA was purified by HPLC as described by Kariko et al., except that mRNA was recovered from HPLC fractions by RNeasy purification.

    Cell Culture:

    Article Title: Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation
    Article Snippet: Materials and methods Cell preparation HC11 mouse mammary epithelial cells were cultured in RPMI 1640 medium containing 10% fetal calf serum, 5 µg/ml Insulin, 10 mM Hepes and 10 ng/ml epidermal growth factor (EGF) (Cerrito et al. ). .. RNA preparation RNA was extracted using Trizol reagent (Invitrogen) and RNeasy maxi kit (Qiagen).

    Generated:

    Article Title: Insertion Sequence ISEcp1B Is Involved in Expression and Mobilization of a blaCTX-M ?-Lactamase Gene
    Article Snippet: Total RNAs of E. coli DH10B (pA-1) and E. coli DH10B(pB-1) were extracted using the Qiagen RNeasy maxi-kit. .. The cDNA was generated from the CTX-M-REV primer (Table ), which had been previously 5′ end labeled with [γ-32 P]ATP and polynucleotide kinase.

    Sequencing:

    Article Title: Exosomes harbor B cell targets in pancreatic adenocarcinoma and exert decoy function against complement-mediated cytotoxicity
    Article Snippet: mRNA extraction was performed by using RNeasy Maxi Kit (Qiagen). .. Paired-end sequencing with the read length of 50 bases were performed on the Illumina HiSeq 2000 platform following the manufacturer’s instructions.

    Article Title: Genome-wide distribution of Auts2 binding localizes with active neurodevelopmental genes
    Article Snippet: .. RNA-seq Total RNA was extracted from two replicates of E16.5 mouse forebrain tissue and purified using the RNeasy Maxi Kit (Qiagen, Venlo, Limburg, The Netherlands) and sent to Otogenetics for ribosomal RNA depletion, cDNA production using random primers, library preparation, and pair end sequencing (~100 bp) using Illumina HiSeq2000. ..

    Article Title: Phenotypic and genetic characterization of a novel phenotype in pigs characterized by juvenile hairlessness and age dependent emphysema
    Article Snippet: .. ITGB6 coding sequence Total RNA was isolated from lung tissue samples from two hh piglets using Qiagen (Germany) RNeasy Maxi kit according to the supplier's instructions. .. Four micrograms of the total RNA were incubated for 30 min at 37°C with RQ1 RNase-Free Dnase (Promega, USA) in a 20-μl reaction.

    Sonication:

    Article Title: Dual Promoters Are Responsible for Transcription Initiation of the fla/che Operon in Bacillus subtilis
    Article Snippet: .. Total RNA was extracted by using the RNeasy Maxi RNA isolation kit (Qiagen) as recommended by the manufacturer except that cells were disrupted by sonication. ..

    In Vivo:

    Article Title: Dual Promoters Are Responsible for Transcription Initiation of the fla/che Operon in Bacillus subtilis
    Article Snippet: To determine if promoter consensus sequences identified by computer analyses are functional in vivo, total RNA was purified from the appropriate strains and subjected to primer extension analyses. .. Total RNA was extracted by using the RNeasy Maxi RNA isolation kit (Qiagen) as recommended by the manufacturer except that cells were disrupted by sonication.

    RNA Sequencing Assay:

    Article Title: Exosomes harbor B cell targets in pancreatic adenocarcinoma and exert decoy function against complement-mediated cytotoxicity
    Article Snippet: mRNA extraction was performed by using RNeasy Maxi Kit (Qiagen). .. RNA-Seq libraries were prepared by using standard Illumina reagents and protocols.

    Article Title: Genome-wide distribution of Auts2 binding localizes with active neurodevelopmental genes
    Article Snippet: .. RNA-seq Total RNA was extracted from two replicates of E16.5 mouse forebrain tissue and purified using the RNeasy Maxi Kit (Qiagen, Venlo, Limburg, The Netherlands) and sent to Otogenetics for ribosomal RNA depletion, cDNA production using random primers, library preparation, and pair end sequencing (~100 bp) using Illumina HiSeq2000. ..

    Isolation:

    Article Title: A high‐throughput method to identify trans‐activation domains within transcription factor sequences
    Article Snippet: .. Total RNA was isolated using Qiagen RNeasy Maxi or Mini Prep Kit for GFP‐negative and GFP‐positive cells, respectively. .. Poly‐A+ RNA purification was performed using Invitrogen Dynabeads Oligo‐dT25 (cat. no. 610‐05) followed by Ambion TURBO DNase (cat. no. AM2239) treatment according to the manufacturer's protocols, also described previously in Ref (Arnold et al , ).

    Article Title: Inflammatory Proprotein Convertase-Matrix Metalloproteinase Proteolytic Pathway in Antigen-presenting Cells as a Step to Autoimmune Multiple Sclerosis *
    Article Snippet: .. Total RNA was isolated from the cells using the RNeasy Maxi kit (Qiagen). .. RNA (3.0 μg) was primed with oligo(dT) and then reverse-transcribed using the QuantiTech reverse transcription kit (Qiagen).

    Article Title: Dual Promoters Are Responsible for Transcription Initiation of the fla/che Operon in Bacillus subtilis
    Article Snippet: .. Total RNA was extracted by using the RNeasy Maxi RNA isolation kit (Qiagen) as recommended by the manufacturer except that cells were disrupted by sonication. ..

    Article Title: A Novel Xylosylphosphotransferase Activity Discovered in Cryptococcus neoformans *
    Article Snippet: The upper aqueous phase was mixed with an equal volume of 70% ethanol and applied to an RNeasy® Maxi column (RNeasy® Maxi Kit, Qiagen). .. Total RNA was isolated according to the manufacturer's protocol and used to generate cDNA using the SuperscriptTM first strand synthesis system for reverse transcription-PCR (Invitrogen).

    Article Title: Phenotypic and genetic characterization of a novel phenotype in pigs characterized by juvenile hairlessness and age dependent emphysema
    Article Snippet: .. ITGB6 coding sequence Total RNA was isolated from lung tissue samples from two hh piglets using Qiagen (Germany) RNeasy Maxi kit according to the supplier's instructions. .. Four micrograms of the total RNA were incubated for 30 min at 37°C with RQ1 RNase-Free Dnase (Promega, USA) in a 20-μl reaction.

    RNA Extraction:

    Article Title: ?-Synuclein Increases the Cellular Level of Phospholipase C?1
    Article Snippet: Paragraph title: 2.6 RNA extraction, cDNA synthesis and real-time PCR analysis ... Total RNA was extracted from the cell lysate using the RNeasy Maxi Kit (Qiagen, Valencia, CA) with DNAse I treatment to eliminate genomic DNA contamination.

    Article Title: Insertion Sequence ISEcp1B Is Involved in Expression and Mobilization of a blaCTX-M ?-Lactamase Gene
    Article Snippet: Paragraph title: RNA extraction and primer extension analysis. ... Total RNAs of E. coli DH10B (pA-1) and E. coli DH10B(pB-1) were extracted using the Qiagen RNeasy maxi-kit.

    Article Title: Dual Promoters Are Responsible for Transcription Initiation of the fla/che Operon in Bacillus subtilis
    Article Snippet: Paragraph title: RNA extraction and primer extension analysis. ... Total RNA was extracted by using the RNeasy Maxi RNA isolation kit (Qiagen) as recommended by the manufacturer except that cells were disrupted by sonication.

    Labeling:

    Article Title: Insertion Sequence ISEcp1B Is Involved in Expression and Mobilization of a blaCTX-M ?-Lactamase Gene
    Article Snippet: Total RNAs of E. coli DH10B (pA-1) and E. coli DH10B(pB-1) were extracted using the Qiagen RNeasy maxi-kit. .. The cDNA was generated from the CTX-M-REV primer (Table ), which had been previously 5′ end labeled with [γ-32 P]ATP and polynucleotide kinase.

    Article Title: Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation
    Article Snippet: RNA preparation RNA was extracted using Trizol reagent (Invitrogen) and RNeasy maxi kit (Qiagen). .. Labeling, hybridization and analysis Mouse (Development) Oligo Microarrays were purchased from Agilent Technologies.

    Purification:

    Article Title: A high‐throughput method to identify trans‐activation domains within transcription factor sequences
    Article Snippet: Total RNA was isolated using Qiagen RNeasy Maxi or Mini Prep Kit for GFP‐negative and GFP‐positive cells, respectively. .. Poly‐A+ RNA purification was performed using Invitrogen Dynabeads Oligo‐dT25 (cat. no. 610‐05) followed by Ambion TURBO DNase (cat. no. AM2239) treatment according to the manufacturer's protocols, also described previously in Ref (Arnold et al , ).

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification
    Article Snippet: .. We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy. .. A portion of each mRNA was purified by HPLC as described by Kariko et al., except that mRNA was recovered from HPLC fractions by RNeasy purification.

    Article Title: Genome-wide distribution of Auts2 binding localizes with active neurodevelopmental genes
    Article Snippet: .. RNA-seq Total RNA was extracted from two replicates of E16.5 mouse forebrain tissue and purified using the RNeasy Maxi Kit (Qiagen, Venlo, Limburg, The Netherlands) and sent to Otogenetics for ribosomal RNA depletion, cDNA production using random primers, library preparation, and pair end sequencing (~100 bp) using Illumina HiSeq2000. ..

    Article Title: Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation
    Article Snippet: RNA preparation RNA was extracted using Trizol reagent (Invitrogen) and RNeasy maxi kit (Qiagen). .. Alternatively, the RNA was purified to recover mRNA using Oligtex mRNA kit.

    Article Title: Dual Promoters Are Responsible for Transcription Initiation of the fla/che Operon in Bacillus subtilis
    Article Snippet: To determine if promoter consensus sequences identified by computer analyses are functional in vivo, total RNA was purified from the appropriate strains and subjected to primer extension analyses. .. Total RNA was extracted by using the RNeasy Maxi RNA isolation kit (Qiagen) as recommended by the manufacturer except that cells were disrupted by sonication.

    Polymerase Chain Reaction:

    Article Title: ?-Synuclein Increases the Cellular Level of Phospholipase C?1
    Article Snippet: Total RNA was extracted from the cell lysate using the RNeasy Maxi Kit (Qiagen, Valencia, CA) with DNAse I treatment to eliminate genomic DNA contamination. .. The cDNA samples were diluted 1:16 and real-time PCR was performed using the QuantiTect SyBr Green PCR kit (Qiagen, Valencia, CA) in an ABI 7300 Real Time PCR System (Applied Biosystems,).

    Article Title: Inflammatory Proprotein Convertase-Matrix Metalloproteinase Proteolytic Pathway in Antigen-presenting Cells as a Step to Autoimmune Multiple Sclerosis *
    Article Snippet: Total RNA was isolated from the cells using the RNeasy Maxi kit (Qiagen). .. The cDNA (25 ng) and the primers (400 n m ) were used in the duplicate 30-μl reactions with the 1× SYBR Green PCR Master Mix (Applied Biosystems).

    Article Title: A Novel Xylosylphosphotransferase Activity Discovered in Cryptococcus neoformans *
    Article Snippet: The upper aqueous phase was mixed with an equal volume of 70% ethanol and applied to an RNeasy® Maxi column (RNeasy® Maxi Kit, Qiagen). .. Each was PCR-amplified from JEC21 cDNA using primer pairs that introduced SpeI restriction sites at both ends (CNJ001/CNJ002 and MCR035/MCR036).

    Spectroscopy:

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification
    Article Snippet: We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy. .. Transcription quality was measured by bioanalyzer analysis (Agilent 2100 Bioanalyzer). mRNA concentrations were measured by UV spectroscopy and corrected for predicted extinction coefficient.

    Plasmid Preparation:

    Article Title: A Novel Xylosylphosphotransferase Activity Discovered in Cryptococcus neoformans *
    Article Snippet: I-SceI digestion linearizes the plasmid while exposing telomeric sequences ( ). .. The upper aqueous phase was mixed with an equal volume of 70% ethanol and applied to an RNeasy® Maxi column (RNeasy® Maxi Kit, Qiagen).

    Real-time Polymerase Chain Reaction:

    Article Title: ?-Synuclein Increases the Cellular Level of Phospholipase C?1
    Article Snippet: Paragraph title: 2.6 RNA extraction, cDNA synthesis and real-time PCR analysis ... Total RNA was extracted from the cell lysate using the RNeasy Maxi Kit (Qiagen, Valencia, CA) with DNAse I treatment to eliminate genomic DNA contamination.

    Functional Assay:

    Article Title: Dual Promoters Are Responsible for Transcription Initiation of the fla/che Operon in Bacillus subtilis
    Article Snippet: To determine if promoter consensus sequences identified by computer analyses are functional in vivo, total RNA was purified from the appropriate strains and subjected to primer extension analyses. .. Total RNA was extracted by using the RNeasy Maxi RNA isolation kit (Qiagen) as recommended by the manufacturer except that cells were disrupted by sonication.

    In Vitro:

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification
    Article Snippet: Paragraph title: In Vitro Transcription of Modified mRNAs ... We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy.

    Spectrophotometry:

    Article Title: ?-Synuclein Increases the Cellular Level of Phospholipase C?1
    Article Snippet: Total RNA was extracted from the cell lysate using the RNeasy Maxi Kit (Qiagen, Valencia, CA) with DNAse I treatment to eliminate genomic DNA contamination. .. The RNA was quantitated by spectrophotometry, the samples were diluted to nominally identical concentrations and then quantitated a second time.

    Article Title: Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation
    Article Snippet: RNA preparation RNA was extracted using Trizol reagent (Invitrogen) and RNeasy maxi kit (Qiagen). .. RNA samples were concentrated to greater than 1 mg/ml by centrifugation on a MicroCon 100 filter unit at 500 g. The concentration and 260/280 OD ratio of the RNA was determined by spectrophotometry and RNA was stored at −80°C.

    Concentration Assay:

    Article Title: Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation
    Article Snippet: RNA preparation RNA was extracted using Trizol reagent (Invitrogen) and RNeasy maxi kit (Qiagen). .. RNA samples were concentrated to greater than 1 mg/ml by centrifugation on a MicroCon 100 filter unit at 500 g. The concentration and 260/280 OD ratio of the RNA was determined by spectrophotometry and RNA was stored at −80°C.

    Lysis:

    Article Title: ?-Synuclein Increases the Cellular Level of Phospholipase C?1
    Article Snippet: Briefly, control HEK cells and HEK cells overexpressing α-synuclein were rinsed twice in PBS prior to lysis in RLT buffer containing 1% β-mercaptoethanol (Qiagen, Valencia, CA). .. Total RNA was extracted from the cell lysate using the RNeasy Maxi Kit (Qiagen, Valencia, CA) with DNAse I treatment to eliminate genomic DNA contamination.

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  • 99
    Qiagen rneasy maxi
    Indel Formation in CD34+ HSPCs Nucleofected with Modified Cas9 <t>mRNAs</t> CD34+ HSPCs were nucleofected with 3 μg of the indicated Cas9 mRNA and 2 μg IL2RGlocus MS-sgRNA. 6 μg Cas9 RNP complexed to 3.2 μg IL2RGlocus MS-sgRNA was nucleofected for comparison. ARCA 5meC/Ψ is our previously published Cas9 mRNA 6 and was also included for comparison. Cells were isolated after 4 days, and indel formation was assessed by TIDE analysis. Bars represent mean ± SEM of at least 5 independent transfections. White and gray bars indicate <t>RNeasy</t> and HPLC-purified mRNAs, respectively. sgRNA complexed to Cas9 RNP was included as a control. ***p
    Rneasy Maxi, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy maxi/product/Qiagen
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    rneasy maxi - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    86
    Qiagen p aeruginosa mus
    Schematic representations of the genetic environment of the bla OXA-18 gene (A) and the bla OXA-20 ) in P. <t>aeruginosa</t> <t>MUS.</t> The coding regions are shown as boxes, with an arrow indicating the orientation of transcription. Black circles indicate
    P Aeruginosa Mus, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p aeruginosa mus - by Bioz Stars, 2020-04
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    Indel Formation in CD34+ HSPCs Nucleofected with Modified Cas9 mRNAs CD34+ HSPCs were nucleofected with 3 μg of the indicated Cas9 mRNA and 2 μg IL2RGlocus MS-sgRNA. 6 μg Cas9 RNP complexed to 3.2 μg IL2RGlocus MS-sgRNA was nucleofected for comparison. ARCA 5meC/Ψ is our previously published Cas9 mRNA 6 and was also included for comparison. Cells were isolated after 4 days, and indel formation was assessed by TIDE analysis. Bars represent mean ± SEM of at least 5 independent transfections. White and gray bars indicate RNeasy and HPLC-purified mRNAs, respectively. sgRNA complexed to Cas9 RNP was included as a control. ***p

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

    doi: 10.1016/j.omtn.2018.06.010

    Figure Lengend Snippet: Indel Formation in CD34+ HSPCs Nucleofected with Modified Cas9 mRNAs CD34+ HSPCs were nucleofected with 3 μg of the indicated Cas9 mRNA and 2 μg IL2RGlocus MS-sgRNA. 6 μg Cas9 RNP complexed to 3.2 μg IL2RGlocus MS-sgRNA was nucleofected for comparison. ARCA 5meC/Ψ is our previously published Cas9 mRNA 6 and was also included for comparison. Cells were isolated after 4 days, and indel formation was assessed by TIDE analysis. Bars represent mean ± SEM of at least 5 independent transfections. White and gray bars indicate RNeasy and HPLC-purified mRNAs, respectively. sgRNA complexed to Cas9 RNP was included as a control. ***p

    Article Snippet: We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy.

    Techniques: Modification, Mass Spectrometry, Isolation, Transfection, High Performance Liquid Chromatography, Purification

    Indel Formation in CD34+ HSPCs Nucleofected with Modified Cas9 mRNAs CD34+ HSPCs were nucleofected with 3 μg of the indicated Cas9 mRNA and 2 μg IL2RGlocus MS-sgRNA. 6 μg Cas9 RNP complexed to 3.2 μg IL2RGlocus MS-sgRNA was nucleofected for comparison. ARCA 5meC/Ψ is our previously published Cas9 and was also included for comparison. Cells were isolated after 4 days, and indel formation was assessed by TIDE analysis. Bars represent mean ± SEM of at least 5 independent transfections. White and gray bars indicate RNeasy and HPLC-purified mRNAs, respectively. sgRNA complexed to Cas9 RNP was included as a control. ***p

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

    doi: 10.1016/j.omtn.2018.06.010

    Figure Lengend Snippet: Indel Formation in CD34+ HSPCs Nucleofected with Modified Cas9 mRNAs CD34+ HSPCs were nucleofected with 3 μg of the indicated Cas9 mRNA and 2 μg IL2RGlocus MS-sgRNA. 6 μg Cas9 RNP complexed to 3.2 μg IL2RGlocus MS-sgRNA was nucleofected for comparison. ARCA 5meC/Ψ is our previously published Cas9 and was also included for comparison. Cells were isolated after 4 days, and indel formation was assessed by TIDE analysis. Bars represent mean ± SEM of at least 5 independent transfections. White and gray bars indicate RNeasy and HPLC-purified mRNAs, respectively. sgRNA complexed to Cas9 RNP was included as a control. ***p

    Article Snippet: We initially made anti-reverse CleanCap trimers with a 3′-O-methyl group on the sugar of the m7 G to prevent incorporation in the opposite orientation, but we found this to be unnecessary, as the 3′-O-methyl version functioned equivalently to CleanCap with a 3′ OH. mRNAs were purified by RNeasy Maxi (QIAGEN, 75162), phosphatase treated for 1 hr with final 0.25 U/μg Antarctic phosphatase (M0289L) in 1× Antarctic phosphatase buffer, and then re-purified by RNeasy.

    Techniques: Modification, Mass Spectrometry, Isolation, Transfection, High Performance Liquid Chromatography, Purification

    Schematic representations of the genetic environment of the bla OXA-18 gene (A) and the bla OXA-20 ) in P. aeruginosa MUS. The coding regions are shown as boxes, with an arrow indicating the orientation of transcription. Black circles indicate

    Journal:

    Article Title: Genetic Structure Associated with blaOXA-18, Encoding a Clavulanic Acid-Inhibited Extended-Spectrum Oxacillinase ▿

    doi: 10.1128/AAC.00403-08

    Figure Lengend Snippet: Schematic representations of the genetic environment of the bla OXA-18 gene (A) and the bla OXA-20 ) in P. aeruginosa MUS. The coding regions are shown as boxes, with an arrow indicating the orientation of transcription. Black circles indicate

    Article Snippet: Then, 5 μg of total RNAs extracted from P. aeruginosa MUS (Qiagen RNeasy Maxi kit) was used to determine the bla OXA-18 initiation site of transcription.

    Techniques:

    Molecular comparison of bla OXA-18 -producing P. aeruginosa isolates. (A and B) PFGE with SpeI-restricted DNA (A) and bla OXA-18 hybridization of the SpeI PFGE gel (B). Lane 1, P. aeruginosa MUS; lane 2, P. aeruginosa 1-63; lane 3, P. aeruginosa 1-22; lane

    Journal:

    Article Title: Genetic Structure Associated with blaOXA-18, Encoding a Clavulanic Acid-Inhibited Extended-Spectrum Oxacillinase ▿

    doi: 10.1128/AAC.00403-08

    Figure Lengend Snippet: Molecular comparison of bla OXA-18 -producing P. aeruginosa isolates. (A and B) PFGE with SpeI-restricted DNA (A) and bla OXA-18 hybridization of the SpeI PFGE gel (B). Lane 1, P. aeruginosa MUS; lane 2, P. aeruginosa 1-63; lane 3, P. aeruginosa 1-22; lane

    Article Snippet: Then, 5 μg of total RNAs extracted from P. aeruginosa MUS (Qiagen RNeasy Maxi kit) was used to determine the bla OXA-18 initiation site of transcription.

    Techniques: Hybridization