rneasy kit  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Qiagen rneasy kit
    Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 997 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy kit/product/Qiagen
    Average 99 stars, based on 997 article reviews
    Price from $9.99 to $1999.99
    rneasy kit - by Bioz Stars, 2020-01
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing
    Article Snippet: Feasibility Study by cDNA Mixing Two cell clones (A and B) expressing a human IgG with different light chain (LC) sequences were thawed from frozen banks and cultured in alpha-MEM (Gibco, Cat. 12561) containing 10% dialyzed fetal bovine serum (FBS, SAFC, Cat. 12015C) and 0.45% glucose (Sigma, Cat. G8769). .. RNA extraction was performed using the RNeasy kit (Qiagen, Cat. 74104), and RNA was eluted in 50 μ L RNase-free water.

    Centrifugation:

    Article Title: The lateral meningocele syndrome mutation causes marked osteopenia in mice
    Article Snippet: .. Total RNA was extracted from cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized tibiae or osteocyte-enriched femoral fragments, following the removal of the bone marrow by centrifugation, with the micro RNeasy kit (Qiagen), in accordance with the manufacturer's instructions, as reported previously ( , , , ). .. The integrity of the RNA from tibiae and osteocyte-rich fragments was assessed by microfluidic electrophoresis on an Experion instrument (Bio-Rad), and only RNA with a quality indicator number equal to or higher than 7.0 was used for subsequent analysis.

    Amplification:

    Article Title: The lateral meningocele syndrome mutation causes marked osteopenia in mice
    Article Snippet: Total RNA was extracted from cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized tibiae or osteocyte-enriched femoral fragments, following the removal of the bone marrow by centrifugation, with the micro RNeasy kit (Qiagen), in accordance with the manufacturer's instructions, as reported previously ( , , , ). .. Equal amounts of RNA were reverse transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (Integrated DNA Technologies) ( ) with iQ SYBR Green Supermix or SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 °C for 35 cycles.

    Article Title: MICE HARBORING A HAJDU CHENEY SYNDROME MUTATION ARE SENSITIZED TO OSTEOARTHRITIS
    Article Snippet: Total RNA was extracted from chondrocyte-enriched cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized femoral heads with the micro RNeasy kit (Qiagen), in accordance with manufacturer’s instructions. .. Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (all from IDT; ) with the iQ SYBR Green Supermix (Bio-Rad) at 60°C for 35 cycles [ , ].

    Synthesized:

    Article Title: Essential Role of Mitochondrial Ca2+ Uniporter in the Generation of Mitochondrial pH Gradient and Metabolism-Secretion Coupling in Insulin-releasing Cells *
    Article Snippet: Total RNA was isolated from cells 72 h after siRNA transfection using the RNeasy kit (Cat. 74134, Qiagen GmbH, Hilden, Germany). .. First strand cDNA was synthesized from 1 μg of total RNA with a reverse transcription kit (Applied Bioscience, Foster City, CA) using oligo-dT.

    Quantitative RT-PCR:

    Article Title: MICE HARBORING A HAJDU CHENEY SYNDROME MUTATION ARE SENSITIZED TO OSTEOARTHRITIS
    Article Snippet: Paragraph title: 2.6. RNA Integrity and Quantitative Reverse Transcription-PCR (qRT-PCR) ... Total RNA was extracted from chondrocyte-enriched cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized femoral heads with the micro RNeasy kit (Qiagen), in accordance with manufacturer’s instructions.

    Article Title: Sustained Notch2 signaling in osteoblasts, but not in osteoclasts, is linked to osteopenia in a mouse model of Hajdu-Cheney syndrome
    Article Snippet: Paragraph title: RNA integrity and qRT-PCR ... Total RNA was extracted from osteoblast-enriched cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized bones with the micro RNeasy kit (Qiagen), in accordance with manufacturer's instructions.

    Article Title: Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply
    Article Snippet: Paragraph title: Quantitative RT-PCR ... RNA was isolated from 5 dpf zebrafish embryos using an RNeasy kit (Qiagen, Cat. 74104) and cDNA was reverse transcribed using an EcoRry Premix (Takara-Clontech, Cat. 639543).

    Article Title: Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply
    Article Snippet: .. Quantitative RT-PCR RNA was isolated from 5 dpf zebrafish embryos using an RNeasy kit (Qiagen, Cat. 74104) and cDNA was reverse transcribed using an EcoRry Premix (Takara-Clontech, Cat. 639543). .. Quantitative PCR (Kapa SYBR FAST qPCR kit, Cat. KK4602) was performed using a Rotor Gene Q qPCR machine (Qiagen).

    Article Title: Angiogenesis Is Induced and Wound Size Is Reduced by Electrical Stimulation in an Acute Wound Healing Model in Human Skin
    Article Snippet: RNA isolation, cDNA synthesis and quantitative real time- polymerase chain reaction For in vivo biopsies obtained on different days of healing, RNA isolation, cDNA synthesis, and qRT-PCR were performed as explained previously [ ]. .. The mixture was then spun to get the upper aqueous layer and mixed with an equal volume of 70% ethanol, which was then further processed with RNeasy kit (cat. 74106; Qiagen) according to the manufacturer’s instructions to extract total RNA.

    SYBR Green Assay:

    Article Title: The lateral meningocele syndrome mutation causes marked osteopenia in mice
    Article Snippet: Total RNA was extracted from cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized tibiae or osteocyte-enriched femoral fragments, following the removal of the bone marrow by centrifugation, with the micro RNeasy kit (Qiagen), in accordance with the manufacturer's instructions, as reported previously ( , , , ). .. Equal amounts of RNA were reverse transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (Integrated DNA Technologies) ( ) with iQ SYBR Green Supermix or SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 °C for 35 cycles.

    Article Title: MICE HARBORING A HAJDU CHENEY SYNDROME MUTATION ARE SENSITIZED TO OSTEOARTHRITIS
    Article Snippet: Total RNA was extracted from chondrocyte-enriched cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized femoral heads with the micro RNeasy kit (Qiagen), in accordance with manufacturer’s instructions. .. Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (all from IDT; ) with the iQ SYBR Green Supermix (Bio-Rad) at 60°C for 35 cycles [ , ].

    Incubation:

    Article Title: Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing
    Article Snippet: RNA extraction was performed using the RNeasy kit (Qiagen, Cat. 74104), and RNA was eluted in 50 μ L RNase-free water. .. RT-PCR was run on the Applied Biosystems 2720 Thermal Cycler with incubation periods of 30 min at 50°C and 15 min at 95°C, 30 cycles of 30-second denaturing at 94°C, 30-second annealing at 62°C, and 2 min extension at 72°C, followed by final 10 min incubation at 72°C. cDNA was purified using the Qiaquick PCR Purification Kit (Qiagen, Cat. 28106) and eluted in 30 μ L EB buffer (10 mM Tris-Cl, pH 8.5). cDNA concentrations were measured on NanoDrop.

    Article Title: Choroid Sprouting Assay: An Ex Vivo Model of Microvascular Angiogenesis
    Article Snippet: .. Real-time PCR After 6 days of incubation, the sprouting cells from choroid and aortic ring explants were isolated as described for FACS analysis. mRNA of the sprouts was extracted by RNeasy® kit (Qiagen, Cat. 74104) following the manufacture's protocol. .. Total RNA (1–5 µg per sample) was diluted into a volume of 15 µL and incubated at 70°C for 10 minutes to dissociate the secondary structure.

    Expressing:

    Article Title: Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing
    Article Snippet: Feasibility Study by cDNA Mixing Two cell clones (A and B) expressing a human IgG with different light chain (LC) sequences were thawed from frozen banks and cultured in alpha-MEM (Gibco, Cat. 12561) containing 10% dialyzed fetal bovine serum (FBS, SAFC, Cat. 12015C) and 0.45% glucose (Sigma, Cat. G8769). .. RNA extraction was performed using the RNeasy kit (Qiagen, Cat. 74104), and RNA was eluted in 50 μ L RNase-free water.

    Article Title: Fingolimod effects in neuroinflammation: Regulation of astroglial glutamate transporters?
    Article Snippet: Real-time PCR Gene expression was analysed by real time PCR. .. Cultured astrocytes were lysed in RLT-buffer (RNeasy kit, QIAGEN, Hilden, Germany) and total RNA was isolated using the RNeasy kit following the manufacturer’s instructions (QIAGEN).

    Article Title: Choroid Sprouting Assay: An Ex Vivo Model of Microvascular Angiogenesis
    Article Snippet: Real-time PCR After 6 days of incubation, the sprouting cells from choroid and aortic ring explants were isolated as described for FACS analysis. mRNA of the sprouts was extracted by RNeasy® kit (Qiagen, Cat. 74104) following the manufacture's protocol. .. These cDNA samples were then mixed with 2× QuantiTect SYBER PCR Master Mix and primer pairs for the quantitative measurement of corresponding gene expression.

    Article Title: Angiogenesis Is Induced and Wound Size Is Reduced by Electrical Stimulation in an Acute Wound Healing Model in Human Skin
    Article Snippet: The mixture was then spun to get the upper aqueous layer and mixed with an equal volume of 70% ethanol, which was then further processed with RNeasy kit (cat. 74106; Qiagen) according to the manufacturer’s instructions to extract total RNA. .. RPL32 was used as the internal control and ΔΔCT method was used to calculate fold change of gene expression.

    Real-time Polymerase Chain Reaction:

    Article Title: Essential Role of Mitochondrial Ca2+ Uniporter in the Generation of Mitochondrial pH Gradient and Metabolism-Secretion Coupling in Insulin-releasing Cells *
    Article Snippet: Paragraph title: Quantitative Real-time PCR ... Total RNA was isolated from cells 72 h after siRNA transfection using the RNeasy kit (Cat. 74134, Qiagen GmbH, Hilden, Germany).

    Article Title: Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply
    Article Snippet: RNA was isolated from 5 dpf zebrafish embryos using an RNeasy kit (Qiagen, Cat. 74104) and cDNA was reverse transcribed using an EcoRry Premix (Takara-Clontech, Cat. 639543). .. Quantitative PCR (Kapa SYBR FAST qPCR kit, Cat. KK4602) was performed using a Rotor Gene Q qPCR machine (Qiagen).

    Article Title: Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply
    Article Snippet: Quantitative RT-PCR RNA was isolated from 5 dpf zebrafish embryos using an RNeasy kit (Qiagen, Cat. 74104) and cDNA was reverse transcribed using an EcoRry Premix (Takara-Clontech, Cat. 639543). .. Quantitative PCR (Kapa SYBR FAST qPCR kit, Cat. KK4602) was performed using a Rotor Gene Q qPCR machine (Qiagen).

    Article Title: Fingolimod effects in neuroinflammation: Regulation of astroglial glutamate transporters?
    Article Snippet: Paragraph title: Real-time PCR ... Cultured astrocytes were lysed in RLT-buffer (RNeasy kit, QIAGEN, Hilden, Germany) and total RNA was isolated using the RNeasy kit following the manufacturer’s instructions (QIAGEN).

    Article Title: Choroid Sprouting Assay: An Ex Vivo Model of Microvascular Angiogenesis
    Article Snippet: .. Real-time PCR After 6 days of incubation, the sprouting cells from choroid and aortic ring explants were isolated as described for FACS analysis. mRNA of the sprouts was extracted by RNeasy® kit (Qiagen, Cat. 74104) following the manufacture's protocol. .. Total RNA (1–5 µg per sample) was diluted into a volume of 15 µL and incubated at 70°C for 10 minutes to dissociate the secondary structure.

    Article Title: Angiogenesis Is Induced and Wound Size Is Reduced by Electrical Stimulation in an Acute Wound Healing Model in Human Skin
    Article Snippet: Paragraph title: RNA isolation, cDNA synthesis and quantitative real time- polymerase chain reaction ... The mixture was then spun to get the upper aqueous layer and mixed with an equal volume of 70% ethanol, which was then further processed with RNeasy kit (cat. 74106; Qiagen) according to the manufacturer’s instructions to extract total RNA.

    Transfection:

    Article Title: Essential Role of Mitochondrial Ca2+ Uniporter in the Generation of Mitochondrial pH Gradient and Metabolism-Secretion Coupling in Insulin-releasing Cells *
    Article Snippet: .. Total RNA was isolated from cells 72 h after siRNA transfection using the RNeasy kit (Cat. 74134, Qiagen GmbH, Hilden, Germany). .. First strand cDNA was synthesized from 1 μg of total RNA with a reverse transcription kit (Applied Bioscience, Foster City, CA) using oligo-dT.

    Sequencing:

    Article Title: Essential Role of Mitochondrial Ca2+ Uniporter in the Generation of Mitochondrial pH Gradient and Metabolism-Secretion Coupling in Insulin-releasing Cells *
    Article Snippet: Total RNA was isolated from cells 72 h after siRNA transfection using the RNeasy kit (Cat. 74134, Qiagen GmbH, Hilden, Germany). .. Quantitative PCR was performed using sequence-specific primers for rat MCU (forward: 5′-GAAGTAGGTGACCGGTTCCA-3′, reverse: 5′-AGGAAAGCGGAGAAGAGGAC-3′), and LETM1 (forward: 5′-GGCTGGACTTGCACCTGTAT-3′, reverse: 5′-CAAGGTGGACTTCAGCTGGT-3′).

    Serial Dilution:

    Article Title: The lateral meningocele syndrome mutation causes marked osteopenia in mice
    Article Snippet: Total RNA was extracted from cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized tibiae or osteocyte-enriched femoral fragments, following the removal of the bone marrow by centrifugation, with the micro RNeasy kit (Qiagen), in accordance with the manufacturer's instructions, as reported previously ( , , , ). .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Bglap (encoding for osteocalcin; from J. Lian, University of Vermont, Burlington, VT), Hey1 and Hey2 (both from T. Iso, Gunma University, Gunma, Japan), HeyL (from D. Srivastava, Gladstone Institute of Cardiovascular Disease, San Francisco, CA), Tnfsf11 (from Source BioScience, Nottingham, UK), or Tnfrsf11b (from American Type Tissue Culture Collection (ATCC), Manassas, VA) ( , ).

    Article Title: MICE HARBORING A HAJDU CHENEY SYNDROME MUTATION ARE SENSITIZED TO OSTEOARTHRITIS
    Article Snippet: Total RNA was extracted from chondrocyte-enriched cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized femoral heads with the micro RNeasy kit (Qiagen), in accordance with manufacturer’s instructions. .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Il6 (Thermo Scientific), Hes1 and Rpl38 (from American Type Culture Collection; ATCC, Manassas, VA), Hey1 and Hey2 (T. Iso, Los Angeles, CA) and HeyL (D. Srivastava, Dallas, TX) [ , ].

    Cell Culture:

    Article Title: Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing
    Article Snippet: Feasibility Study by cDNA Mixing Two cell clones (A and B) expressing a human IgG with different light chain (LC) sequences were thawed from frozen banks and cultured in alpha-MEM (Gibco, Cat. 12561) containing 10% dialyzed fetal bovine serum (FBS, SAFC, Cat. 12015C) and 0.45% glucose (Sigma, Cat. G8769). .. RNA extraction was performed using the RNeasy kit (Qiagen, Cat. 74104), and RNA was eluted in 50 μ L RNase-free water.

    Article Title: Fingolimod effects in neuroinflammation: Regulation of astroglial glutamate transporters?
    Article Snippet: .. Cultured astrocytes were lysed in RLT-buffer (RNeasy kit, QIAGEN, Hilden, Germany) and total RNA was isolated using the RNeasy kit following the manufacturer’s instructions (QIAGEN). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing
    Article Snippet: RNA extraction was performed using the RNeasy kit (Qiagen, Cat. 74104), and RNA was eluted in 50 μ L RNase-free water. .. RT-PCR of IgG light chains was set up with 200 ng RNA per sample using the OneStep RT-PCR kit (Qiagen, Cat. 210212) in 50 μ L reaction volume.

    Article Title: The lateral meningocele syndrome mutation causes marked osteopenia in mice
    Article Snippet: Paragraph title: Quantitative reverse transcription–polymerase chain reaction ... Total RNA was extracted from cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized tibiae or osteocyte-enriched femoral fragments, following the removal of the bone marrow by centrifugation, with the micro RNeasy kit (Qiagen), in accordance with the manufacturer's instructions, as reported previously ( , , , ).

    Article Title: MICE HARBORING A HAJDU CHENEY SYNDROME MUTATION ARE SENSITIZED TO OSTEOARTHRITIS
    Article Snippet: Total RNA was extracted from chondrocyte-enriched cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized femoral heads with the micro RNeasy kit (Qiagen), in accordance with manufacturer’s instructions. .. Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (all from IDT; ) with the iQ SYBR Green Supermix (Bio-Rad) at 60°C for 35 cycles [ , ].

    Article Title: Sustained Notch2 signaling in osteoblasts, but not in osteoclasts, is linked to osteopenia in a mouse model of Hajdu-Cheney syndrome
    Article Snippet: Total RNA was extracted from osteoblast-enriched cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized bones with the micro RNeasy kit (Qiagen), in accordance with manufacturer's instructions. .. Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (all primers from IDT; ) with the iQ SYBR Green Supermix (Bio-Rad) at 60 °C for 35 cycles.

    In Vivo:

    Article Title: Angiogenesis Is Induced and Wound Size Is Reduced by Electrical Stimulation in an Acute Wound Healing Model in Human Skin
    Article Snippet: RNA isolation, cDNA synthesis and quantitative real time- polymerase chain reaction For in vivo biopsies obtained on different days of healing, RNA isolation, cDNA synthesis, and qRT-PCR were performed as explained previously [ ]. .. The mixture was then spun to get the upper aqueous layer and mixed with an equal volume of 70% ethanol, which was then further processed with RNeasy kit (cat. 74106; Qiagen) according to the manufacturer’s instructions to extract total RNA.

    Isolation:

    Article Title: Essential Role of Mitochondrial Ca2+ Uniporter in the Generation of Mitochondrial pH Gradient and Metabolism-Secretion Coupling in Insulin-releasing Cells *
    Article Snippet: .. Total RNA was isolated from cells 72 h after siRNA transfection using the RNeasy kit (Cat. 74134, Qiagen GmbH, Hilden, Germany). .. First strand cDNA was synthesized from 1 μg of total RNA with a reverse transcription kit (Applied Bioscience, Foster City, CA) using oligo-dT.

    Article Title: Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply
    Article Snippet: .. RNA was isolated from 5 dpf zebrafish embryos using an RNeasy kit (Qiagen, Cat. 74104) and cDNA was reverse transcribed using an EcoRry Premix (Takara-Clontech, Cat. 639543). .. Quantitative PCR (Kapa SYBR FAST qPCR kit, Cat. KK4602) was performed using a Rotor Gene Q qPCR machine (Qiagen).

    Article Title: Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply
    Article Snippet: .. Quantitative RT-PCR RNA was isolated from 5 dpf zebrafish embryos using an RNeasy kit (Qiagen, Cat. 74104) and cDNA was reverse transcribed using an EcoRry Premix (Takara-Clontech, Cat. 639543). .. Quantitative PCR (Kapa SYBR FAST qPCR kit, Cat. KK4602) was performed using a Rotor Gene Q qPCR machine (Qiagen).

    Article Title: Fingolimod effects in neuroinflammation: Regulation of astroglial glutamate transporters?
    Article Snippet: .. Cultured astrocytes were lysed in RLT-buffer (RNeasy kit, QIAGEN, Hilden, Germany) and total RNA was isolated using the RNeasy kit following the manufacturer’s instructions (QIAGEN). ..

    Article Title: Choroid Sprouting Assay: An Ex Vivo Model of Microvascular Angiogenesis
    Article Snippet: .. Real-time PCR After 6 days of incubation, the sprouting cells from choroid and aortic ring explants were isolated as described for FACS analysis. mRNA of the sprouts was extracted by RNeasy® kit (Qiagen, Cat. 74104) following the manufacture's protocol. .. Total RNA (1–5 µg per sample) was diluted into a volume of 15 µL and incubated at 70°C for 10 minutes to dissociate the secondary structure.

    Article Title: Angiogenesis Is Induced and Wound Size Is Reduced by Electrical Stimulation in an Acute Wound Healing Model in Human Skin
    Article Snippet: Paragraph title: RNA isolation, cDNA synthesis and quantitative real time- polymerase chain reaction ... The mixture was then spun to get the upper aqueous layer and mixed with an equal volume of 70% ethanol, which was then further processed with RNeasy kit (cat. 74106; Qiagen) according to the manufacturer’s instructions to extract total RNA.

    Purification:

    Article Title: Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing
    Article Snippet: RNA extraction was performed using the RNeasy kit (Qiagen, Cat. 74104), and RNA was eluted in 50 μ L RNase-free water. .. RT-PCR was run on the Applied Biosystems 2720 Thermal Cycler with incubation periods of 30 min at 50°C and 15 min at 95°C, 30 cycles of 30-second denaturing at 94°C, 30-second annealing at 62°C, and 2 min extension at 72°C, followed by final 10 min incubation at 72°C. cDNA was purified using the Qiaquick PCR Purification Kit (Qiagen, Cat. 28106) and eluted in 30 μ L EB buffer (10 mM Tris-Cl, pH 8.5). cDNA concentrations were measured on NanoDrop.

    Polymerase Chain Reaction:

    Article Title: Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing
    Article Snippet: RNA extraction was performed using the RNeasy kit (Qiagen, Cat. 74104), and RNA was eluted in 50 μ L RNase-free water. .. RT-PCR was run on the Applied Biosystems 2720 Thermal Cycler with incubation periods of 30 min at 50°C and 15 min at 95°C, 30 cycles of 30-second denaturing at 94°C, 30-second annealing at 62°C, and 2 min extension at 72°C, followed by final 10 min incubation at 72°C. cDNA was purified using the Qiaquick PCR Purification Kit (Qiagen, Cat. 28106) and eluted in 30 μ L EB buffer (10 mM Tris-Cl, pH 8.5). cDNA concentrations were measured on NanoDrop.

    Article Title: Fingolimod effects in neuroinflammation: Regulation of astroglial glutamate transporters?
    Article Snippet: Cultured astrocytes were lysed in RLT-buffer (RNeasy kit, QIAGEN, Hilden, Germany) and total RNA was isolated using the RNeasy kit following the manufacturer’s instructions (QIAGEN). .. PCR reactions were performed at a 5 µl scale on a qTower real time PCR System (Analytic Jena) in triplicates.

    Article Title: Choroid Sprouting Assay: An Ex Vivo Model of Microvascular Angiogenesis
    Article Snippet: Real-time PCR After 6 days of incubation, the sprouting cells from choroid and aortic ring explants were isolated as described for FACS analysis. mRNA of the sprouts was extracted by RNeasy® kit (Qiagen, Cat. 74104) following the manufacture's protocol. .. These cDNA samples were then mixed with 2× QuantiTect SYBER PCR Master Mix and primer pairs for the quantitative measurement of corresponding gene expression.

    Electrophoresis:

    Article Title: The lateral meningocele syndrome mutation causes marked osteopenia in mice
    Article Snippet: Total RNA was extracted from cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized tibiae or osteocyte-enriched femoral fragments, following the removal of the bone marrow by centrifugation, with the micro RNeasy kit (Qiagen), in accordance with the manufacturer's instructions, as reported previously ( , , , ). .. The integrity of the RNA from tibiae and osteocyte-rich fragments was assessed by microfluidic electrophoresis on an Experion instrument (Bio-Rad), and only RNA with a quality indicator number equal to or higher than 7.0 was used for subsequent analysis.

    Article Title: MICE HARBORING A HAJDU CHENEY SYNDROME MUTATION ARE SENSITIZED TO OSTEOARTHRITIS
    Article Snippet: Total RNA was extracted from chondrocyte-enriched cells with the RNeasy kit (Qiagen, Valencia, CA) and from homogenized femoral heads with the micro RNeasy kit (Qiagen), in accordance with manufacturer’s instructions. .. The integrity of the RNA was assessed by microfluidic electrophoresis on an Experion system (Bio-Rad, Hercules, CA), and only RNA with a quality indicator number equal to or higher than 7.0 was used for subsequent analysis.

    RNA Extraction:

    Article Title: Mutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing
    Article Snippet: .. RNA extraction was performed using the RNeasy kit (Qiagen, Cat. 74104), and RNA was eluted in 50 μ L RNase-free water. ..

    Homogenization:

    Article Title: Angiogenesis Is Induced and Wound Size Is Reduced by Electrical Stimulation in an Acute Wound Healing Model in Human Skin
    Article Snippet: Briefly, after tissues were diced, 0·5–1 ml Trizol was added and the tubes were introduced to Qiagen tissue lyser (Qiagen, Crawley, UK) to homogenization. .. The mixture was then spun to get the upper aqueous layer and mixed with an equal volume of 70% ethanol, which was then further processed with RNeasy kit (cat. 74106; Qiagen) according to the manufacturer’s instructions to extract total RNA.

    FACS:

    Article Title: Choroid Sprouting Assay: An Ex Vivo Model of Microvascular Angiogenesis
    Article Snippet: .. Real-time PCR After 6 days of incubation, the sprouting cells from choroid and aortic ring explants were isolated as described for FACS analysis. mRNA of the sprouts was extracted by RNeasy® kit (Qiagen, Cat. 74104) following the manufacture's protocol. .. Total RNA (1–5 µg per sample) was diluted into a volume of 15 µL and incubated at 70°C for 10 minutes to dissociate the secondary structure.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Qiagen rneasy plus mini kit
    Rneasy Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plus mini kit/product/Qiagen
    Average 90 stars, based on 841 article reviews
    Price from $9.99 to $1999.99
    rneasy plus mini kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Qiagen rneasy micro kit
    Rneasy Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 2018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy micro kit/product/Qiagen
    Average 90 stars, based on 2018 article reviews
    Price from $9.99 to $1999.99
    rneasy micro kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Qiagen rneasy mini kit
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 8038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy mini kit/product/Qiagen
    Average 90 stars, based on 8038 article reviews
    Price from $9.99 to $1999.99
    rneasy mini kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results