rnease plus micro kit  (Qiagen)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    RNeasy Plus Micro Kit
    Description:
    For purification of up to 45 µg total RNA from cells tissues using gDNA Eliminator columns Kit contents Qiagen RNeasy Plus Micro Kit 50 preps 14L Elution Volume 5mg Tissue Amount Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes RNeasy MinElute Spin Columns gDNA Eliminator Spin Columns Collection Tubes Carrier RNA RNase free Water and Buffers Benefits Unique gDNA Eliminator columns avoid the need for DNase Efficient removal of genomic DNA Highly reproducible yields of RNA in minutes High performance RNA for sensitive application
    Catalog Number:
    74034
    Price:
    436
    Category:
    RNeasy Plus Micro Kit
    Buy from Supplier


    Structured Review

    Qiagen rnease plus micro kit
    RNeasy Plus Micro Kit
    For purification of up to 45 µg total RNA from cells tissues using gDNA Eliminator columns Kit contents Qiagen RNeasy Plus Micro Kit 50 preps 14L Elution Volume 5mg Tissue Amount Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes RNeasy MinElute Spin Columns gDNA Eliminator Spin Columns Collection Tubes Carrier RNA RNase free Water and Buffers Benefits Unique gDNA Eliminator columns avoid the need for DNase Efficient removal of genomic DNA Highly reproducible yields of RNA in minutes High performance RNA for sensitive application
    https://www.bioz.com/result/rnease plus micro kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnease plus micro kit - by Bioz Stars, 2021-01
    99/100 stars

    Related Products / Commonly Used Together

    rna lysis buffer

    Images

    Related Articles

    RNA Extraction:

    Article Title: Mov34 Protein from Mouse Brain Interacts with the 3? Noncoding Region of Japanese Encephalitis Virus
    Article Snippet: .. Total RNA from neonatal mouse brain tissue was isolated using a commercially available RNA extraction kit (RNeasy; Qiagen). cDNA to the Mov34 gene was made using avian myeloblastosis virus reverse transcriptase and a synthetic oligonucleotide, SV173 (CA AAGCTT A CTTTTTCTCCTTTTTCTC ), which contained the Hin dIII restriction site (italics) and an 18-base complementary sequence (underlined) derived from the 3′ end of the Mov34 coding sequence ( ). .. The Mov34 cDNA was PCR amplified using SV173 and SV172 (TGT GGATCC ATGCCGGAGCTGGCGGTG ), which contained Mov34-specific sequence from the 5′ end of the gene (underlined) and a Bam HI restriction site (italics).

    Article Title: Biosynthesis of Lipoic Acid in Arabidopsis: Cloning and Characterization of the cDNA for Lipoic Acid Synthase 1
    Article Snippet: .. Total RNAs used for cDNA synthesis and RT-PCR were extracted from leaves, roots, and flowers of Arabidopsis using an RNA-extraction kit (RNeasy plant kit, Qiagen, Chatsworth, CA). .. RT-PCR analysis of the LIP1 gene was performed with an RT-PCR kit (SuperScript OneStep System, Life Technologies) using the following two primers: 5′-TTGGGGATACATGTACACGC-3′ and 5′-CTGAATCCCATTTCCATGCC-3′.

    Sequencing:

    Article Title: Mov34 Protein from Mouse Brain Interacts with the 3? Noncoding Region of Japanese Encephalitis Virus
    Article Snippet: .. Total RNA from neonatal mouse brain tissue was isolated using a commercially available RNA extraction kit (RNeasy; Qiagen). cDNA to the Mov34 gene was made using avian myeloblastosis virus reverse transcriptase and a synthetic oligonucleotide, SV173 (CA AAGCTT A CTTTTTCTCCTTTTTCTC ), which contained the Hin dIII restriction site (italics) and an 18-base complementary sequence (underlined) derived from the 3′ end of the Mov34 coding sequence ( ). .. The Mov34 cDNA was PCR amplified using SV173 and SV172 (TGT GGATCC ATGCCGGAGCTGGCGGTG ), which contained Mov34-specific sequence from the 5′ end of the gene (underlined) and a Bam HI restriction site (italics).

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
    Article Snippet: .. High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples. .. Sequencing libraries were prepared using the ScriptSeq V2 RNA-seq Kit (Epicentre), again using the low input protocol.

    RNA Sequencing Assay:

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
    Article Snippet: .. High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples. .. Sequencing libraries were prepared using the ScriptSeq V2 RNA-seq Kit (Epicentre), again using the low input protocol.

    Isolation:

    Article Title: Mov34 Protein from Mouse Brain Interacts with the 3? Noncoding Region of Japanese Encephalitis Virus
    Article Snippet: .. Total RNA from neonatal mouse brain tissue was isolated using a commercially available RNA extraction kit (RNeasy; Qiagen). cDNA to the Mov34 gene was made using avian myeloblastosis virus reverse transcriptase and a synthetic oligonucleotide, SV173 (CA AAGCTT A CTTTTTCTCCTTTTTCTC ), which contained the Hin dIII restriction site (italics) and an 18-base complementary sequence (underlined) derived from the 3′ end of the Mov34 coding sequence ( ). .. The Mov34 cDNA was PCR amplified using SV173 and SV172 (TGT GGATCC ATGCCGGAGCTGGCGGTG ), which contained Mov34-specific sequence from the 5′ end of the gene (underlined) and a Bam HI restriction site (italics).

    Article Title: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability
    Article Snippet: .. Real time quantitative PCR Total RNA was extracted from FAC sorted GFP+ cells using RNA column-based isolation kits, RNeasy® Micro kit (Qiagen). .. Concentration and integrity of the RNA extracted from FAC sorted cells were determined with an Agilent 2100 Bioanalyzer and by use of the Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
    Article Snippet: .. The RNAqueous Micro Total RNA Isolation Kit from Ambion and the RNeasy Plus Micro Kit from Qiagen are specifically designed for purification of total RNA from a small amount of cells ( < 200,000 cells). .. In both cases, only longer RNA fragments ( > 200 nucleotides) are purified, although the workflow can be modified to isolate small RNAs such as microRNAs, 5,8S rRNA, 5S RNA, tRNA’s,… (Additional file : Figure S1).

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
    Article Snippet: .. Based on different quality parameters and comparison of various experimental set-ups, we recommend using the RNeasy Plus Micro Kit for RNA isolation of FACS sorted zebrafish cells. .. The RNA purification protocol is fast (only 20–30 min), easy and consistently delivers high-quality RNA samples.

    Article Title: Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-related Macular Degeneration
    Article Snippet: .. To confirm that no residual RPE donor cells contaminated the hiPSC cultures, total RNA was isolated (Cat. # 74034; Qiagen) from each hiPSC line, cDNA was reverse transcribed (Cat. # 4387406; Thermo Fisher) and qPCR performed using primers specific for the RPE gene RPE65 ( ). .. hiPSC lines were grown on irradiated mouse embryonic feeders (Cat. # GSE-6301G; GlobalStem) in serum-free medium supplemented with 4ng/ml FGF2 (Cat. # 233-FB; R & D systems).

    Purification:

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
    Article Snippet: .. The RNAqueous Micro Total RNA Isolation Kit from Ambion and the RNeasy Plus Micro Kit from Qiagen are specifically designed for purification of total RNA from a small amount of cells ( < 200,000 cells). .. In both cases, only longer RNA fragments ( > 200 nucleotides) are purified, although the workflow can be modified to isolate small RNAs such as microRNAs, 5,8S rRNA, 5S RNA, tRNA’s,… (Additional file : Figure S1).

    Real-time Polymerase Chain Reaction:

    Article Title: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability
    Article Snippet: .. Real time quantitative PCR Total RNA was extracted from FAC sorted GFP+ cells using RNA column-based isolation kits, RNeasy® Micro kit (Qiagen). .. Concentration and integrity of the RNA extracted from FAC sorted cells were determined with an Agilent 2100 Bioanalyzer and by use of the Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-related Macular Degeneration
    Article Snippet: .. To confirm that no residual RPE donor cells contaminated the hiPSC cultures, total RNA was isolated (Cat. # 74034; Qiagen) from each hiPSC line, cDNA was reverse transcribed (Cat. # 4387406; Thermo Fisher) and qPCR performed using primers specific for the RPE gene RPE65 ( ). .. hiPSC lines were grown on irradiated mouse embryonic feeders (Cat. # GSE-6301G; GlobalStem) in serum-free medium supplemented with 4ng/ml FGF2 (Cat. # 233-FB; R & D systems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Biosynthesis of Lipoic Acid in Arabidopsis: Cloning and Characterization of the cDNA for Lipoic Acid Synthase 1
    Article Snippet: .. Total RNAs used for cDNA synthesis and RT-PCR were extracted from leaves, roots, and flowers of Arabidopsis using an RNA-extraction kit (RNeasy plant kit, Qiagen, Chatsworth, CA). .. RT-PCR analysis of the LIP1 gene was performed with an RT-PCR kit (SuperScript OneStep System, Life Technologies) using the following two primers: 5′-TTGGGGATACATGTACACGC-3′ and 5′-CTGAATCCCATTTCCATGCC-3′.

    High Throughput Screening Assay:

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
    Article Snippet: .. High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples. .. Sequencing libraries were prepared using the ScriptSeq V2 RNA-seq Kit (Epicentre), again using the low input protocol.

    FACS:

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
    Article Snippet: .. Based on different quality parameters and comparison of various experimental set-ups, we recommend using the RNeasy Plus Micro Kit for RNA isolation of FACS sorted zebrafish cells. .. The RNA purification protocol is fast (only 20–30 min), easy and consistently delivers high-quality RNA samples.

    Derivative Assay:

    Article Title: Mov34 Protein from Mouse Brain Interacts with the 3? Noncoding Region of Japanese Encephalitis Virus
    Article Snippet: .. Total RNA from neonatal mouse brain tissue was isolated using a commercially available RNA extraction kit (RNeasy; Qiagen). cDNA to the Mov34 gene was made using avian myeloblastosis virus reverse transcriptase and a synthetic oligonucleotide, SV173 (CA AAGCTT A CTTTTTCTCCTTTTTCTC ), which contained the Hin dIII restriction site (italics) and an 18-base complementary sequence (underlined) derived from the 3′ end of the Mov34 coding sequence ( ). .. The Mov34 cDNA was PCR amplified using SV173 and SV172 (TGT GGATCC ATGCCGGAGCTGGCGGTG ), which contained Mov34-specific sequence from the 5′ end of the gene (underlined) and a Bam HI restriction site (italics).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen rna extraction kit
    Organ-specific expression of the LIP1 gene. The level of LIP1 mRNA for lipoic acid synthase was analyzed by <t>RT-PCR</t> with total RNAs extracted from leaves (lane 2), roots (lane 3), and flowers (lane 4). In lane 1, genomic DNA was used as the template for RT-PCR instead of <t>RNA.</t> The level of mRNA for the cytosolic form of cyclophilin was also analyzed as a control with the same total RNAs extracted from leaves (lane 5), roots (lane 6), and flowers (lane 7). The positions of the DNA size markers (in kb) are indicated on the left.
    Rna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna extraction kit/product/Qiagen
    Average 99 stars, based on 391 article reviews
    Price from $9.99 to $1999.99
    rna extraction kit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    95
    Qiagen aurum total rna mini kit
    Bioanalyzer Agilent electrophoresis runs. Examples of representative Bioanalyzer Agilent electrophoresis runs for the five different methods applied for <t>RNA</t> extractions from P . lividus embryos: <t>TRIzol,</t> GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich), RNAqueous® Micro Kit (Ambion from Life Technologies), RNeasy® Micro Kit (Qiagen) and Aurum™ Total RNA Mini Kit (Biorad). Four different numerical amounts of embryos were used for RNA extraction: 500, 1000, 2500 and 5000 embryos. The ladder (L) is reported in the first lane of each run. The green band at the bottom of each panel is the RNA 6000 Nano Marker (Agilent RNA 6000 Nano Kit, Agilent Technologies, Inc.). Red box in the 500 lane of TRIzol indicates that the Bioanalyzer software cannot calculate RIN values (reported as N/A in the Table 1 ) for this sample, because of very low concentration and high level of degradation of the RNA.
    Aurum Total Rna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aurum total rna mini kit/product/Qiagen
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    aurum total rna mini kit - by Bioz Stars, 2021-01
    95/100 stars
      Buy from Supplier

    Image Search Results


    Organ-specific expression of the LIP1 gene. The level of LIP1 mRNA for lipoic acid synthase was analyzed by RT-PCR with total RNAs extracted from leaves (lane 2), roots (lane 3), and flowers (lane 4). In lane 1, genomic DNA was used as the template for RT-PCR instead of RNA. The level of mRNA for the cytosolic form of cyclophilin was also analyzed as a control with the same total RNAs extracted from leaves (lane 5), roots (lane 6), and flowers (lane 7). The positions of the DNA size markers (in kb) are indicated on the left.

    Journal: Plant Physiology

    Article Title: Biosynthesis of Lipoic Acid in Arabidopsis: Cloning and Characterization of the cDNA for Lipoic Acid Synthase 1

    doi:

    Figure Lengend Snippet: Organ-specific expression of the LIP1 gene. The level of LIP1 mRNA for lipoic acid synthase was analyzed by RT-PCR with total RNAs extracted from leaves (lane 2), roots (lane 3), and flowers (lane 4). In lane 1, genomic DNA was used as the template for RT-PCR instead of RNA. The level of mRNA for the cytosolic form of cyclophilin was also analyzed as a control with the same total RNAs extracted from leaves (lane 5), roots (lane 6), and flowers (lane 7). The positions of the DNA size markers (in kb) are indicated on the left.

    Article Snippet: Total RNAs used for cDNA synthesis and RT-PCR were extracted from leaves, roots, and flowers of Arabidopsis using an RNA-extraction kit (RNeasy plant kit, Qiagen, Chatsworth, CA).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Islet1 knock-down affects sensory neuron differentiation. (A-C) In control embryos (A, B) , Rohon-Beard cells (RBs) express runx3 . Islet1 knock-down leads to fewer cells with robust expression of runx3 (C). The asterisk denotes a cell expressing the gene. (D-F’) Tg(-3.4neurog1:gfp)sb4 control (D-E’) and E3 morphant (F, F’) 24 hours post-fertilization (hpf) embryos were examined for expression of olig4 (red). (D-E’) In lateral views of the dorsal spinal cord, RNA in situ hybridization reveals expression of the interneuron marker olig4 (red). Green fluorescent protein (GFP) + neurons do not express olig4 and comprise RBs and dorsal lateral ascending interneurons (see Figure 2 ). (F-F’) Islet1 knock-down leads to an increase in the number of olig4 expressing cells within the dorsal spinal cord. However, similar to RBs of control embryos (D-E’) , RB-like neurons do not express detectable levels of olig4 (F) . (G-I) At 72 hpf, dorsal root ganglia (DRGs) are easily identified as GFP + neurons with large somata located near the spinal cord/notochord border. (G, H) In control embryos, DRG neurons project from their soma bipolar axons that extend dorsally and ventrally (asterisks). (I) Islet1 knock-down reduces the number of GFP + DRGs. Furthermore, for the few GFP + DRGs remaining, their axons show abnormal morphologies. Scale bars = 50 μm in A (for A-C ), D (for D-F ’) and G (for G-I ). CtlMO, 5-base mismatched; islet1 (Sp)E3MO; E3MO, E3 morpholino; Uninj, uninjected.

    Journal: Neural Development

    Article Title: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability

    doi: 10.1186/1749-8104-9-19

    Figure Lengend Snippet: Islet1 knock-down affects sensory neuron differentiation. (A-C) In control embryos (A, B) , Rohon-Beard cells (RBs) express runx3 . Islet1 knock-down leads to fewer cells with robust expression of runx3 (C). The asterisk denotes a cell expressing the gene. (D-F’) Tg(-3.4neurog1:gfp)sb4 control (D-E’) and E3 morphant (F, F’) 24 hours post-fertilization (hpf) embryos were examined for expression of olig4 (red). (D-E’) In lateral views of the dorsal spinal cord, RNA in situ hybridization reveals expression of the interneuron marker olig4 (red). Green fluorescent protein (GFP) + neurons do not express olig4 and comprise RBs and dorsal lateral ascending interneurons (see Figure 2 ). (F-F’) Islet1 knock-down leads to an increase in the number of olig4 expressing cells within the dorsal spinal cord. However, similar to RBs of control embryos (D-E’) , RB-like neurons do not express detectable levels of olig4 (F) . (G-I) At 72 hpf, dorsal root ganglia (DRGs) are easily identified as GFP + neurons with large somata located near the spinal cord/notochord border. (G, H) In control embryos, DRG neurons project from their soma bipolar axons that extend dorsally and ventrally (asterisks). (I) Islet1 knock-down reduces the number of GFP + DRGs. Furthermore, for the few GFP + DRGs remaining, their axons show abnormal morphologies. Scale bars = 50 μm in A (for A-C ), D (for D-F ’) and G (for G-I ). CtlMO, 5-base mismatched; islet1 (Sp)E3MO; E3MO, E3 morpholino; Uninj, uninjected.

    Article Snippet: Real time quantitative PCR Total RNA was extracted from FAC sorted GFP+ cells using RNA column-based isolation kits, RNeasy® Micro kit (Qiagen).

    Techniques: Expressing, RNA In Situ Hybridization, Marker

    Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Journal: Scientific Reports

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    doi: 10.1038/s41598-018-25921-8

    Figure Lengend Snippet: Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Article Snippet: High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples.

    Techniques: Expressing, Over Expression, RNA Sequencing Assay, Plasmid Preparation

    Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Journal: Scientific Reports

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    doi: 10.1038/s41598-018-25921-8

    Figure Lengend Snippet: Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Article Snippet: High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples.

    Techniques: Expressing, Over Expression, RNA Sequencing Assay

    Bioanalyzer Agilent electrophoresis runs. Examples of representative Bioanalyzer Agilent electrophoresis runs for the five different methods applied for RNA extractions from P . lividus embryos: TRIzol, GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich), RNAqueous® Micro Kit (Ambion from Life Technologies), RNeasy® Micro Kit (Qiagen) and Aurum™ Total RNA Mini Kit (Biorad). Four different numerical amounts of embryos were used for RNA extraction: 500, 1000, 2500 and 5000 embryos. The ladder (L) is reported in the first lane of each run. The green band at the bottom of each panel is the RNA 6000 Nano Marker (Agilent RNA 6000 Nano Kit, Agilent Technologies, Inc.). Red box in the 500 lane of TRIzol indicates that the Bioanalyzer software cannot calculate RIN values (reported as N/A in the Table 1 ) for this sample, because of very low concentration and high level of degradation of the RNA.

    Journal: PLoS ONE

    Article Title: High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos

    doi: 10.1371/journal.pone.0172171

    Figure Lengend Snippet: Bioanalyzer Agilent electrophoresis runs. Examples of representative Bioanalyzer Agilent electrophoresis runs for the five different methods applied for RNA extractions from P . lividus embryos: TRIzol, GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich), RNAqueous® Micro Kit (Ambion from Life Technologies), RNeasy® Micro Kit (Qiagen) and Aurum™ Total RNA Mini Kit (Biorad). Four different numerical amounts of embryos were used for RNA extraction: 500, 1000, 2500 and 5000 embryos. The ladder (L) is reported in the first lane of each run. The green band at the bottom of each panel is the RNA 6000 Nano Marker (Agilent RNA 6000 Nano Kit, Agilent Technologies, Inc.). Red box in the 500 lane of TRIzol indicates that the Bioanalyzer software cannot calculate RIN values (reported as N/A in the Table 1 ) for this sample, because of very low concentration and high level of degradation of the RNA.

    Article Snippet: Asai et al. [ ] compared three commonly-used methods: TRIzol® , Aurum Total RNA Mini Kit and Qiagen RNeasy Micro Kit, in combination with preservation reagents TRIzol® or RNAlate r® , to obtain high-quality and large quantities of RNA from the copepod Calanus helgolandicus .

    Techniques: Electrophoresis, RNA Extraction, Marker, Software, Concentration Assay

    Agilent Bioanlyzer electropherograms. Examples of representative Agilent Bioanlyzer electropherograms of P . lividus RNA: for TRIzol, GenElute and RNAqueous RNA extraction from 5000 embryos extraction; for RNeasy and Aurum RNA extraction from 2500 embryos (see also Table 1 ). Relative Fluorescent Unit (FU) and seconds of migration (s) of RNA samples isolated according to the five different extraction methods are reported. RIN values are also reported.

    Journal: PLoS ONE

    Article Title: High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos

    doi: 10.1371/journal.pone.0172171

    Figure Lengend Snippet: Agilent Bioanlyzer electropherograms. Examples of representative Agilent Bioanlyzer electropherograms of P . lividus RNA: for TRIzol, GenElute and RNAqueous RNA extraction from 5000 embryos extraction; for RNeasy and Aurum RNA extraction from 2500 embryos (see also Table 1 ). Relative Fluorescent Unit (FU) and seconds of migration (s) of RNA samples isolated according to the five different extraction methods are reported. RIN values are also reported.

    Article Snippet: Asai et al. [ ] compared three commonly-used methods: TRIzol® , Aurum Total RNA Mini Kit and Qiagen RNeasy Micro Kit, in combination with preservation reagents TRIzol® or RNAlate r® , to obtain high-quality and large quantities of RNA from the copepod Calanus helgolandicus .

    Techniques: RNA Extraction, Migration, Isolation