rnease minikit  (Qiagen)


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    Structured Review

    Qiagen rnease minikit
    Rnease Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnease minikit/product/Qiagen
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnease minikit - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

    dnase
    total rna

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    Isolation:

    Article Title: Hepatic and Aortic Arch Expression and Serum Levels of Syndecan-1 in ApoE-/- Mice
    Article Snippet: .. Before the additional purification with an RNease minikit (Qiagen) the isolated total RNA was treated with DNase (Fermentas) (Supplementary Material, Table ). ..

    Incubation:

    Article Title: Hepatic and Aortic Arch Expression and Serum Levels of Syndecan-1 in ApoE-/- Mice
    Article Snippet: To precipitate the RNA, an equal volume of isopropanol was added; the mixture was incubated for 10min at room temperature and then centrifuged at +4°C for 10min at 12000 g . .. Before the additional purification with an RNease minikit (Qiagen) the isolated total RNA was treated with DNase (Fermentas) (Supplementary Material, Table ).

    Purification:

    Article Title: Hepatic and Aortic Arch Expression and Serum Levels of Syndecan-1 in ApoE-/- Mice
    Article Snippet: .. Before the additional purification with an RNease minikit (Qiagen) the isolated total RNA was treated with DNase (Fermentas) (Supplementary Material, Table ). ..

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  • 99
    Qiagen rneasy plant minikit
    Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the <t>RNeasy</t> plant <t>minikit</t> and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.
    Rneasy Plant Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy plant minikit/product/Qiagen
    Average 99 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    rneasy plant minikit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen rneasy minikit
    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen <t>RNeasy</t> <t>minikit.</t>
    Rneasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy minikit/product/Qiagen
    Average 99 stars, based on 2156 article reviews
    Price from $9.99 to $1999.99
    rneasy minikit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the RNeasy plant minikit and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.

    Journal: Journal of Virology

    Article Title: Natural 2?,5?-Phosphodiester Bonds Found at the Ligation Sites of Peach Latent Mosaic Viroid

    doi: 10.1128/JVI.75.1.19-25.2001

    Figure Lengend Snippet: Autoradiogram of 5% PAGE analysis of self-cleavage experiments using either 2′,5′- or 3′,5′-C-PLMVd. Radioactive transcripts (C-PLMVd) were added to the ground leaf powder, and the RNA was extracted using the RNeasy plant minikit and incubated under self-cleavage conditions with or without prior heat denaturation and snap-cooling treatments. Lanes 1 and 6, untreated L-PLMVd (control); lanes 2 to 5 and 7 to 10, 3′,5′- and 2′,5′-C-PLMVd, respectively; lanes 2 and 7, C-PLMVd extracted and incubated under self-cleavage conditions without heat denaturation and snap-cooling steps; lanes 3 and 8, like lanes 2 and 7, except that the extraction was performed in the presence of additional 5 mM EDTA in all buffers; lanes 4 and 9, like lanes 2 and 7, except that the samples were heat denatured and snap-cooled prior to incubation under self-cleavage conditions; lanes 5 and 10, like lanes 4 and 9, except that the extraction was performed in the presence of additional 5 mM EDTA. Adjacent to the gel, the positions of the C-PLMVd and L-PLMVd transcripts are used as size references.

    Article Snippet: The first procedure used an RNeasy plant minikit (Qiagen) to prepare RNA from 50 to 100 mg of tissue as specified by the manufacturer.

    Techniques: Polyacrylamide Gel Electrophoresis, Incubation

    Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.

    Journal: Journal of Virology

    Article Title: Engineering Enhanced Vaccine Cell Lines To Eradicate Vaccine-Preventable Diseases: the Polio End Game

    doi: 10.1128/JVI.01464-15

    Figure Lengend Snippet: Validation of gene silencing in HEp-2C and Vero cells by quantitative PCR (qPCR). siRNAs targeting selected hit genes were transfected into HEp-2C or Vero cells. At 48 h posttransfection, cells were harvested for RNA isolation using a Qiagen RNeasy minikit.

    Article Snippet: Forty-eight hours after siRNA transfection, total RNA was isolated from mock-infected and transfected HEp-2C cells and Vero cells using an RNeasy minikit (Qiagen) according to the manufacturer's protocol.

    Techniques: Real-time Polymerase Chain Reaction, Transfection, Isolation

    The interaction of DDX6 with HCV core and viral RNA is dependent on the C-terminal domain of DDX6. (A) Schematic representation of EYFP-DDX6 and related mutants, ΔC (C-terminal deletion mutant lacking 183 amino acids), EQ (mutation in the DEAD-box helicase motif II involving the substitution of Glu-247 by Gln), and empty vector expressing EYFP alone. The ΔC mutation removes the second of two conserved RecA domains, identified by the shaded bars within the DDX6 sequence. (B) HJ3-5 HCV-infected FT3-7 cells were transfected with DNA vector expressing various forms of EYFP-DDX6 or just the EYFP. Cell lysates were prepared 2 days later and subjected to coimmunoprecipitation with anti-GFP (rabbit polyclonal; Clontech) antibody. Coimmunoprecipitation samples were immunoblotted with GFP (top panel) and core-specific (bottom panel) antibodies. Input represents 1/20 of the immunoprecipitation (IP) for anti-GFP blot and 1/80 of the IP for HCV core blot. Total RNA isolated from immunoprecipitates using an RNeasy minikit (Qiagen) was subjected to RT-PCR for detection of genomic HCV RNA (primers targeting the NS3 region) and GAPDH mRNA. (C) Huh-7-191/20 cells were induced to express HCV core protein by removing tetracycline from the medium for 3 days. Cells were then transfected with various DNAs and subjected to coimmunoprecipitation exactly as described for panel B.

    Journal: Journal of Virology

    Article Title: DDX6 (Rck/p54) Is Required for Efficient Hepatitis C Virus Replication but Not for Internal Ribosome Entry Site-Directed Translation ▿

    doi: 10.1128/JVI.00397-10

    Figure Lengend Snippet: The interaction of DDX6 with HCV core and viral RNA is dependent on the C-terminal domain of DDX6. (A) Schematic representation of EYFP-DDX6 and related mutants, ΔC (C-terminal deletion mutant lacking 183 amino acids), EQ (mutation in the DEAD-box helicase motif II involving the substitution of Glu-247 by Gln), and empty vector expressing EYFP alone. The ΔC mutation removes the second of two conserved RecA domains, identified by the shaded bars within the DDX6 sequence. (B) HJ3-5 HCV-infected FT3-7 cells were transfected with DNA vector expressing various forms of EYFP-DDX6 or just the EYFP. Cell lysates were prepared 2 days later and subjected to coimmunoprecipitation with anti-GFP (rabbit polyclonal; Clontech) antibody. Coimmunoprecipitation samples were immunoblotted with GFP (top panel) and core-specific (bottom panel) antibodies. Input represents 1/20 of the immunoprecipitation (IP) for anti-GFP blot and 1/80 of the IP for HCV core blot. Total RNA isolated from immunoprecipitates using an RNeasy minikit (Qiagen) was subjected to RT-PCR for detection of genomic HCV RNA (primers targeting the NS3 region) and GAPDH mRNA. (C) Huh-7-191/20 cells were induced to express HCV core protein by removing tetracycline from the medium for 3 days. Cells were then transfected with various DNAs and subjected to coimmunoprecipitation exactly as described for panel B.

    Article Snippet: Total RNA was isolated from cells by using the RNeasy minikit (Qiagen) and analyzed using reagents supplied with the NorthernMax kit (Applied Biosystems).

    Techniques: Mutagenesis, Plasmid Preparation, Expressing, Sequencing, Infection, Transfection, Immunoprecipitation, Isolation, Reverse Transcription Polymerase Chain Reaction