rnaseout recombinant ribonuclease inhibitor  (Thermo Fisher)


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    Name:
    RNaseOUT Recombinant Ribonuclease Inhibitor
    Description:
    RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of 52 kDa RNaseOUT inhibits RNase A RNase B and RNase C ApplicationscDNA synthesis RT PCR and in vitro transcription and translationSourcePurified by affinity chromatography from E coli expressing a cloned porcine genePerformance and quality testingSDS PAGE purity endodeoxyribonuclease assay protein concentration specific activity performance evaluated by RT PCRUnit definitionOne unit inhibits 5 ng of RNase A by 50 using cytidine 2 3 cyclic monophosphate cCMP as the substrateUnit reaction conditions100 mM Tris acetate pH 6 5 1 mM EDTA 0 2 mM cCMP 2 µg RNase A in 1 mL from 0 to 10 min at 25°C
    Catalog Number:
    10777019
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher rnaseout recombinant ribonuclease inhibitor
    RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of 52 kDa RNaseOUT inhibits RNase A RNase B and RNase C ApplicationscDNA synthesis RT PCR and in vitro transcription and translationSourcePurified by affinity chromatography from E coli expressing a cloned porcine genePerformance and quality testingSDS PAGE purity endodeoxyribonuclease assay protein concentration specific activity performance evaluated by RT PCRUnit definitionOne unit inhibits 5 ng of RNase A by 50 using cytidine 2 3 cyclic monophosphate cCMP as the substrateUnit reaction conditions100 mM Tris acetate pH 6 5 1 mM EDTA 0 2 mM cCMP 2 µg RNase A in 1 mL from 0 to 10 min at 25°C
    https://www.bioz.com/result/rnaseout recombinant ribonuclease inhibitor/product/Thermo Fisher
    Average 99 stars, based on 363 article reviews
    Price from $9.99 to $1999.99
    rnaseout recombinant ribonuclease inhibitor - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    In Vivo:

    Article Title: The leishmanicidal activity of oleuropein is selectively regulated through inflammation- and oxidative stress-related genes
    Article Snippet: .. Messenger RNA was then reverse-transcribed using a SuperScript II kit (Invitrogen - Molecular Probes™) and oligo-dTs (Promega, WI, USA), and all reactions included the recombinant ribonuclease inhibitor, RNaseOUT™ (Invitrogen). cDNAs of all the in vivo experimental groups were evaluated with real time PCR, performed using an Exicycler 96 thermocycler (Bioneer, Daejeon, Korea), using the Kapa SYBR Fast Universal 2× qPCR Master Mix kit (Kapa Biosystems Ltd., London, UK). .. The specific primers for the interleukin-1α (IL-1α ), interleukin-1β (IL-1β ), interleukin-1 receptor antagonist (IL-1rn ), interleukin-10 (IL-10 ), subunit 2 of interleukin-12 (IL-12β ), interferon-γ (IFN- γ), tumor necrosis factor-α (TNF-α ), transforming growth factor beta-1 (TGF-β1 ), T-box transcription factor (Tbx21 ), trans-acting T-cell-specific transcription factor (GATA3 ), subunit 2 of nuclear factor kappa-B (NF-kB2) , and the glyceraldehyde dehydrogenase of the 3-phosphatase (GAPDH ) gene sequences were designed by Qiagen (QuantiTect Primer Assays; Qiagen, Venlo, Netherlands).

    In Vitro:

    Article Title: Design and application of cotranscriptional non-enzymatic RNA circuits and signal transducers
    Article Snippet: .. In vitro transcription In all, 100 pg to 1000 ng of double-stranded DNA transcription templates was transcribed using 100 U of T7 RNA polymerase (NEB) in 50 µl reactions containing 40 mM Tris–HCl, pH 7.9, 30 mM MgCl2 , 10 mM DTT, 2 mM spermidine, 4 mM ribonucleotide (rNTP) mix and 20 U of the recombinant ribonuclease inhibitor RNaseOUT (Life Technologies). .. Transcription reactions were incubated at 42°C for 1–2 h. After this, transcripts of the circuit components were either (i) used directly for assembly or (ii) subjected to purification before assembly.

    Protease Inhibitor:

    Article Title: miR-205 Expression Promotes Cell Proliferation and Migration of Human Cervical Cancer Cells
    Article Snippet: .. Cell pellet was collected and then re-suspended in an equal volume (w/v) of lysis buffer [FNN0021; Invitrogen; supplemented with 1 mM Phenylmethanesulfonyl fluoride (PMSF, P7626; Sigma-Aldrich), 1 mM Dithiothreitol ( DTT, 495714; Invitrogen), 1% Protease Inhibitor Cocktail (P8340; Sigma-Aldrich) and 200 U/ml RNaseOUT (10777-019; Invitrogen)], incubated for 10 minutes on ice, and lysed by vortexing. ..

    Real-time Polymerase Chain Reaction:

    Article Title: The leishmanicidal activity of oleuropein is selectively regulated through inflammation- and oxidative stress-related genes
    Article Snippet: .. Messenger RNA was then reverse-transcribed using a SuperScript II kit (Invitrogen - Molecular Probes™) and oligo-dTs (Promega, WI, USA), and all reactions included the recombinant ribonuclease inhibitor, RNaseOUT™ (Invitrogen). cDNAs of all the in vivo experimental groups were evaluated with real time PCR, performed using an Exicycler 96 thermocycler (Bioneer, Daejeon, Korea), using the Kapa SYBR Fast Universal 2× qPCR Master Mix kit (Kapa Biosystems Ltd., London, UK). .. The specific primers for the interleukin-1α (IL-1α ), interleukin-1β (IL-1β ), interleukin-1 receptor antagonist (IL-1rn ), interleukin-10 (IL-10 ), subunit 2 of interleukin-12 (IL-12β ), interferon-γ (IFN- γ), tumor necrosis factor-α (TNF-α ), transforming growth factor beta-1 (TGF-β1 ), T-box transcription factor (Tbx21 ), trans-acting T-cell-specific transcription factor (GATA3 ), subunit 2 of nuclear factor kappa-B (NF-kB2) , and the glyceraldehyde dehydrogenase of the 3-phosphatase (GAPDH ) gene sequences were designed by Qiagen (QuantiTect Primer Assays; Qiagen, Venlo, Netherlands).

    Incubation:

    Article Title: An RNA-seq based comparative approach reveals the transcriptome-wide interplay between 3′-to-5′ exoRNases and RNase Y
    Article Snippet: .. RNAs were annealed to the radiolabelled primer (Supplementary Table ) for 30 min at 65 °C and subsequently incubated on ice for 1 min and the RNAs were reverse transcribed using 1U of SuperScript III Reverse Transcriptase (Invitrogen) in the presence of 1X first strand buffer (Invitrogen), 5 mM dithiothreitol (DTT) and 40 U of RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), for 1 h at 55 °C. .. After inhibition of the SuperScript III enzyme by incubation at 70 °C for 15 min, the cDNAs were precipitated with ice-cold 100% ethanol at –20 °C for 1 h and centrifuged at 20,000 × g for 4 °C for 10 min.

    Article Title: miR-205 Expression Promotes Cell Proliferation and Migration of Human Cervical Cancer Cells
    Article Snippet: .. Cell pellet was collected and then re-suspended in an equal volume (w/v) of lysis buffer [FNN0021; Invitrogen; supplemented with 1 mM Phenylmethanesulfonyl fluoride (PMSF, P7626; Sigma-Aldrich), 1 mM Dithiothreitol ( DTT, 495714; Invitrogen), 1% Protease Inhibitor Cocktail (P8340; Sigma-Aldrich) and 200 U/ml RNaseOUT (10777-019; Invitrogen)], incubated for 10 minutes on ice, and lysed by vortexing. ..

    Lysis:

    Article Title: miR-205 Expression Promotes Cell Proliferation and Migration of Human Cervical Cancer Cells
    Article Snippet: .. Cell pellet was collected and then re-suspended in an equal volume (w/v) of lysis buffer [FNN0021; Invitrogen; supplemented with 1 mM Phenylmethanesulfonyl fluoride (PMSF, P7626; Sigma-Aldrich), 1 mM Dithiothreitol ( DTT, 495714; Invitrogen), 1% Protease Inhibitor Cocktail (P8340; Sigma-Aldrich) and 200 U/ml RNaseOUT (10777-019; Invitrogen)], incubated for 10 minutes on ice, and lysed by vortexing. ..

    Recombinant:

    Article Title: The leishmanicidal activity of oleuropein is selectively regulated through inflammation- and oxidative stress-related genes
    Article Snippet: .. Messenger RNA was then reverse-transcribed using a SuperScript II kit (Invitrogen - Molecular Probes™) and oligo-dTs (Promega, WI, USA), and all reactions included the recombinant ribonuclease inhibitor, RNaseOUT™ (Invitrogen). cDNAs of all the in vivo experimental groups were evaluated with real time PCR, performed using an Exicycler 96 thermocycler (Bioneer, Daejeon, Korea), using the Kapa SYBR Fast Universal 2× qPCR Master Mix kit (Kapa Biosystems Ltd., London, UK). .. The specific primers for the interleukin-1α (IL-1α ), interleukin-1β (IL-1β ), interleukin-1 receptor antagonist (IL-1rn ), interleukin-10 (IL-10 ), subunit 2 of interleukin-12 (IL-12β ), interferon-γ (IFN- γ), tumor necrosis factor-α (TNF-α ), transforming growth factor beta-1 (TGF-β1 ), T-box transcription factor (Tbx21 ), trans-acting T-cell-specific transcription factor (GATA3 ), subunit 2 of nuclear factor kappa-B (NF-kB2) , and the glyceraldehyde dehydrogenase of the 3-phosphatase (GAPDH ) gene sequences were designed by Qiagen (QuantiTect Primer Assays; Qiagen, Venlo, Netherlands).

    Article Title: An RNA-seq based comparative approach reveals the transcriptome-wide interplay between 3′-to-5′ exoRNases and RNase Y
    Article Snippet: .. RNAs were annealed to the radiolabelled primer (Supplementary Table ) for 30 min at 65 °C and subsequently incubated on ice for 1 min and the RNAs were reverse transcribed using 1U of SuperScript III Reverse Transcriptase (Invitrogen) in the presence of 1X first strand buffer (Invitrogen), 5 mM dithiothreitol (DTT) and 40 U of RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), for 1 h at 55 °C. .. After inhibition of the SuperScript III enzyme by incubation at 70 °C for 15 min, the cDNAs were precipitated with ice-cold 100% ethanol at –20 °C for 1 h and centrifuged at 20,000 × g for 4 °C for 10 min.

    Article Title: Design and application of cotranscriptional non-enzymatic RNA circuits and signal transducers
    Article Snippet: .. In vitro transcription In all, 100 pg to 1000 ng of double-stranded DNA transcription templates was transcribed using 100 U of T7 RNA polymerase (NEB) in 50 µl reactions containing 40 mM Tris–HCl, pH 7.9, 30 mM MgCl2 , 10 mM DTT, 2 mM spermidine, 4 mM ribonucleotide (rNTP) mix and 20 U of the recombinant ribonuclease inhibitor RNaseOUT (Life Technologies). .. Transcription reactions were incubated at 42°C for 1–2 h. After this, transcripts of the circuit components were either (i) used directly for assembly or (ii) subjected to purification before assembly.

    Article Title: Notch2 Controls Prolactin and Insulin-Like Growth Factor Binding Protein-1 Expression in Decidualizing Human Stromal Cells of Early Pregnancy
    Article Snippet: .. 2 µg RNA were reverse transcribed using 200U of RevertAid H Minus M.MuLV Reverse Transcriptase (Fermentas, St.Leon-Rot, D), 0.2 µg Hexanucleotide Mix (Roche Diagnostics GmbH, Vienna, Austria), 0.5 µl RNaseOUT (Recombinant Ribonuclease Inhibitor, Invitrogen) and 0.5 mM dNTP (Promega, Madison, WI) in a final volume of 20 µl. .. Quantitative real-time PCR was performed on the ABI 7500 Sequence Detection System (Applied Biosystems, Carlsbad, CA) using Taq Man Gene Expression Assays (TaqMan Universal PCR Master Mix, 20x Taq Man Gene Expression Assay Mix for Notch1 (Hs01062014_m1), Notch2 (Hs01050702_m1), Notch3 (Hs01128541_m1), Notch4 (Hs00965889_m1), Jagged1 (Hs01070032_m1), Jagged2 (Hs00171432_m1), DLL1 (Hs00194509_m1), DLL3 (Hs01085096_m1), DLL4 (Hs00184092_m1), HES1 (Hs00172878_m1), PRL (Hs00168730_m1), IGFBP1 (Hs00426285_m1) and TATA-box binding protein (TBP; TaqMan endogenous control)) according to the manufacturer's instructions.

    Article Title: Genome-wide gene expression analysis of anguillid herpesvirus 1
    Article Snippet: .. RNA was extracted according to manufacturer’s protocol, and dissolved in 200 μl RNase-free water (Sigma-Aldrich) with 2 μl RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). .. RNA concentration was checked by spectrophotometry using a Nanodrop ND-1000 (Thermo Fisher Scientific, MA, USA).

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  • 99
    Thermo Fisher rnaseout recombinant ribonuclease inhibitor
    Rnaseout Recombinant Ribonuclease Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnaseout recombinant ribonuclease inhibitor/product/Thermo Fisher
    Average 99 stars, based on 363 article reviews
    Price from $9.99 to $1999.99
    rnaseout recombinant ribonuclease inhibitor - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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